哒嗪酮酸类化合物的合成及其抗惊活性的研究

以ａ－酮戊二酸与水合肼与水合肼为原始原料合成了１５个Ｎ－（３’－磷酸二乙酯丙基）－１，６－二氢－６－氧－３－哒嗪酰胺类化合物，并对它们进行了最大电休克发作（ＭＥＳ）实验，结果表明其中４个化合物表现出中等强度的抗惊活性。     

强噪声对大鼠血液粘度血糖及几种酶的影响

本文检测强噪声刺激12小时大鼠血液流变学九项指标,胆碱酯酶和谷丙转氨酶活力及血糖浓度,与对照组比较,其结果显示:(1)低切粘度,红细胞电泳时间和全血还原粘度明显增高(P<0.05;P<0.05和P<0.01);(2)真性和假性胆碱酯酶活力明显增高(二者均P<0.01);(3)血清谷丙转氨酶活力和血清葡萄糖浓度显著增高(P<0.01和P<0.05)。揭示强噪声作用后机体听觉外系统发生明显变化,长期暴露后必将严重损伤全身性调节功能而引起脏器损害与疾病。     

脊神经节神经元周围突至肾及体壁的分支投射

实验将快蓝(FB)和核黄(NY)分别注入大鼠左肾纤维膜下及左体壁神经干内,结果在荧光镜下发现左T_(9-13),L1脊神经后根节(DRG)内存在双标记细胞,提示大鼠T_(9-13),L1段DRG内的部分神经元的周围突分支支配肾及体壁,为肾绞痛所致牵涉痛的解释,提供了神经解剖学基础。     

溃疡病的病机及临床治疗探讨

本文通过对364例溃疡病患者的住院系统治疗观察,认为溃疡病的主要病机是脾虚。活动期与肝郁及胃热有关;迁延难愈多因胃络瘀阻;胃酸增高为本病的普遍现象。故本病治疗应以健脾胃,佐以疏肝活血、清热、制酸之法。方药如用四君子汤加黄芪、白芍、郁金、三棱、蒲公英、乌贼骨等为基础方加减;对有胆汁返流者,则应配以疏肝清胆,降逆通腑之品。临床近期治愈率可达82.4％。     

APPLICATION OF ACUPOINT-INJECTION THERAPY TO PAIN SYNDROME: CLINICAL ANALYSIS OF 250 CASES

In this paper,250 cases of headache,shoulder pain,lumbago and leg pain weretreated with 20％Angelicae Sinensis mixed with Vitamine B_(12) point-injection therapy.The total effective rate was 95.2％and in which the cure rate was 53.6％.Authors have found a close correlation between the effectiveness and the duration of disease.     

银杏叶提取物中的芦丁含量测定

用紫外分光光度法测定银杏叶提取物中芦丁的含量，结果表明：以６０％丙酮或７０％乙醇提取银杏叶，所得提取物的芦丁含量为３３．２７％～４３．３８％。     

促甲状腺素释放激素拮抗β－内啡肽对心血管的抑制作用

本研究用兔侧脑室给药观察了促甲状腺素释放激素（ＴＲＨ）对β－内啡肽心血管抑制作用的影响。结果表示，β－内啡肽（１０μｇ，ｉｃｖ）能显著抑制兔心血管功能，表现为血压下降，心机收缩力指标如左室收缩压（ＬＶＳＰ），心室内压最大上升和下降速率（±ｄｐ／ｄｔｍａｘ），心肌纤维收缩速度（Ｖｃｅ４０，Ｖｐｍ）和心肌收缩向量环面积（Ｌｐ）明显降低。β－内啡肽的这一作用可被ＴＲＨ（５０μｇ，ｉｃｖ）取消，阿片受体拮抗剂纳洛酮（ＮＡＬ，５０μｇ，ｉｃｖ）也可拮抗β－内啡肽的心血管抑制作用，表明，ＴＲＨ与内源性阿片肽系统有密切关系，结合以前的实验结果提示ＴＲＨ拮抗β－内啡肽的心血管抑制作用有可能是作用在阿片受体上的。     

川芎嗪对环孢素引起的大鼠胰岛β细胞毒性防护作用的实验研究

给大鼠应用环孢素(CsA)50mg/kg·d灌胃,共10天,引起高血糖症及血浆和胰腺组织中胰岛素水平下降。同时联合应用川芎嗪(LIG)50mg/kg·d共10天,能有效地防止和(或)减轻CsA引起的上述变化。LIG还有效地减轻了CsA引起的尿血栓素B_2和尿血栓素B_2/6-酮-前列腺素F_(1α)值的升高。结果提示LIG对CsA引起的胰岛β细胞毒性的防护作用可能与调节前列胰素代谢有关。     

Additive effects of earthworm extract with HpD-laser on the enhancement of reactive oxygen production and the inhibition of[~3H]—thymidine.uptake in mouse ascitic tumor cells

The effects of combined use of earthworm extract(912)and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S_(180) tumor cells were studied throughchemiluminescence measurement and[~3H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2～4 folds.The[~3H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O_2~ and H_2O_2 in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action.     

经口补充谷氨酰胺对烧伤大鼠小肠功能的影响

大鼠２０％ＢＳＡⅢ°烧伤后１４天，门静脉、后腔静脉和腹主动脉血浆谷氨酰胺（Ｌ一Ｇｌｕｔａｍｉｎｅ，Ｇｌｎ）含量均明显降低，小肠粘膜的蛋白质、ＤＮＡ、ＡＴＰ含量及乳糖酶活性均明显低于正常。烧伤后在饲料中补充２％或５％Ｇｌｎ，大鼠血浆Ｇｌｎ水平，小肠粘膜的蛋白质、ＤＮＡ、ＡＴＰ含量，以及乳糖酶活性均明显高于烧伤对照和补充甘氨酸（Ｇｌｙ）组，其中ＡＴＰ含量和乳糖酶活性接近正常组。补充５％Ｇｌｎ的大鼠血浆Ｇｌｎ水平高于２％Ｇｌｎ组，其他指标则并不优于补充２％Ｇｌｎ的动物。上述结果表明，烧伤后经口补充Ｇｌｎ，有助于支持肠道的物质和能量代谢，防治或减轻肠道功能的损伤。