环氧合酶-2和血管内皮生长因子共表达与肝细胞癌血管形成的关系

目的　探讨环氧合酶 (COX) 2与肝细胞癌血管形成的关系。方法　利用免疫组织化学、Westernblot方法检测 48例肝癌组织中COX 2和血管内皮生长因子 (VEGF)蛋白及逆转录 聚合酶链反应法 (RT PCR)检测COX 2和VEGFmRNA的共表达 ,对共表达COX 2和VEGF蛋白和mRNA的肝癌组织进行微血管记数。结果　免疫组织化学检测中 ,48例肝癌组织 3 6例共表达COX 2和VEGF蛋白。类似结果见于蛋白电泳分析。RT PCR显示 ,48例肝癌组织 3 6例共表达COX 2mRNA和VEGFmRNA。两者之间的表达明显相关 (γ =0 .845 )。共表达COX 2和VEGF蛋白和mRNA的肝癌组织中 ,平均微血管数 (5 6.8± 17.5 )个 ,明显高于阴性表达组。结论　COX 2可能与肝细胞癌的血管形成有关 ,且其作用之一可能是通过上调VEGF通道来发挥的     

深低温储皮后其抗原性改变的免疫学研究

以人皮和豚鼠皮新鲜及－１９６℃冷冻皮片匀浆做为抗原，研制出兔抗人和豚鼠的新鲜及－１９６℃冷冻皮片匀浆的可溶性蛋白高效价的免疫血清，并通过免疫电泳、火箭免疫电泳、免疫火箭扩散电泳及单相免疫扩散电泳等不同免疫学电泳的检测，分别对新鲜皮片及－１９６℃冷冻皮片抗原性改变的这一现象进行探讨与研究。结果表明：人皮、豚鼠皮其新鲜皮片的抗原成份、抗原决定簇及抗原量均显著高于相应的－１９６℃冷冻组皮片，因而证实了通过深低温冷冻的皮片其抗原性低于新鲜皮片的论断。     

雷公藤氯内酯醇对大鼠异位心脏移植免疫抑制作用的实验研究

本研究应用纯系大鼠（Ｌｏｕ→Ｆ３４４）心脏移植模型，比较分析了ＣｓＡ和不同剂量Ｔ４治疗组的移植心脏存活时间、病理损害计分以及移植受体大鼠脾单个核细胞ＩＬ－２生成活性和血清ｓＩＬ－２Ｒ水平，证实Ｔ４可明显延长移植心脏存活时间和减少移植排异反应程度。此研究结果表明Ｔ４具有较强的抗移植排异作用。     

Sympathetic skin response in patients with myasthenia gravis★ A comparative analysis

BACKGROUND: The examination of sympathetic skin response is an important index for assessing the autonomic nerve function, and patients with myasthenia gravis are always accompanied by dysautonomia. Therefore, it will be important to know whether sympathetic skin response can be used as the index for the clinical evaluation of myasthenia gravis. OBJECTIVE: To investigate the diagnostic value of sympathetic skin response in the damage of autonomic nerve function of patients with myasthenia gravis. DESIGN: A case-controlled comparative observation. SETTING: Department of Neurology and Room of Nerve Electromyogram, the Affiliated Hospital of North Sichuan Medical College. PARTICIPANTS: Thirty outpatients or inpatients with myasthenia gravis were selected from the Department of Neurology, the Affiliated Hospital of North Sichuan Medical College from May 2006 to May 2007, including 9 males and 21 females, aged 8–72 years with a mean age of (28±5) years old. They were all accorded with the diagnostic standards of myasthenia gravis, accompanied by different severity of autonomic nerve symptoms, including poor skin nutrition, sweating of hands and feet, pyknocardia, persistent hypotension, abdominal pain, constipation, etc. They all had not taken any drug affecting the autonomic nerve function before the examination. Informed consents were obtained from all the patients. Meanwhile, 30 healthy physical examinees were enrolled as the normal control group, including 10 males and 20 females, aged 10–75 years with a mean age of (31±5) years old. Approval was obtained from the hospital ethic committee. METHODS: After admission, the patients were examined with sympathetic skin response using DANTEC keypoint 2.0 electromyography evoked potential apparatus (Danmark). The changes of the latency and wave amplitude of sympathetic skin response were observed. The subjects in the normal control group were examined with the same methods at physical examination. Abnormality was judged by the disappearance of wave form, latency longer than that in the normal control group by Mean±2.5SD, or wave amplitude lower than the average value in the normal control group by 50%. MAIN OUTCOME MEASURES: The results of the latency and wave amplitude of sympathetic skin response were compared between the patients with myasthenia gravis and normal controls. RESULTS: All the 30 patients with myasthenia gravis and 30 healthy physical examinees were involved in the final analysis of results. There were no significant differences between the left and right upper and lower limbs in both the myasthenia gravis group and normal control group (P > 0.05). In the myasthenia gravis group, the abnormal rate of sympathetic skin response was 37% (11/30), the latency was prolonged and the wave amplitude was decreased as compared with those in the normal control group, and there were significant differences (P < 0.01). CONCLUSION: Sympathetic skin response can be used as an electrophysiological index for judging the damages of autonomic nerve function in patients with myasthenia gravis.     

女性尿瘘的治疗体会(附13例报告)

目的　探讨女性尿瘘的诊治,对巨大复杂的膀胱尿道阴道瘘的手术修补法进行探讨。方法　经阴道修补膀胱阴道瘘4例, 尿道阴道瘘3例,膀胱尿道阴道瘘2例,经腹阴道联合途径修补复杂性女性尿瘘4例,其中输尿管阴道瘘1例。结果　一次性修 复痊愈率(甲级)达84.6%、有效率(乙级)达92.3%。结论　术前仔细检查及评估手术方案是缩短手术时间、减少术中创伤的重 要环节,精细的外科手术技巧是确保手术成功的关键,经腹阴道联合手术途径修补术是治疗巨大复杂女性尿瘘的有效方法。     

新基因Tctex5在雄鼠生殖细胞中的表达

目的探讨雄鼠生殖细胞中是否有Tctex5基因的表达。方法从三株体外培养的雄鼠生殖细胞CRL-1715、CRL-2053和CRL-2196细胞株中提取总RNA和蛋白，采用RT-PCR和Western blot分别从mRNA和蛋白质水平分析基因产物的表达。结果Tctex5基因在CRL-1715、CRL-2053、CRL-2196三株细胞中都有高表达。结论Tctex5基因作为一个未知功能的基因，在雄鼠生殖细胞中有丰富的表达，可能参与了精子发生的功能调节，作为男性正常生育的。个新的标志物，它可能在精子发生中扮演了一个重要的角色，该基因在雄性生殖细胞中的发现可能会对男性不育的诊断与治疗提供新的途径。     

短发卡RNA对人胃癌细胞STAT3基因的沉默作用

目的:探讨STAT3基因小发夹RNA（shRNA）表达质粒对胃癌MKN-45细胞STAT3基因的干扰作用。方法:根据STAT3 mRNA 编码序列，设计RNA干扰靶点，构建STAT3基因的特异性小RNA干扰质粒（psiRNA-H1/STAT3），使用脂质体转染人胃癌细胞系（MKN-45细胞）。实验分为对照（A）组，psiRNA-H1转染（B）组和psiRNA-H1/STAT3转染（C）组。通过RT-PCR和Western Blot检测STAT3特异性小RNA干扰基因对胃癌细胞STAT3基因mRNA和蛋白表达的影响。结果:psiRNA-H1/STAT3经限制性酶切及部分序列分析证明基因插入正确，并经测序证实。将其成功转染MKN-45细胞后，该细胞的STAT3 mRNA和蛋白表达均明显下降(P<0.05)。 结论:将成功构建的针对STAT3基因的shRNA表达载体转染MKN-45细胞，能有效抑制该细胞的STAT3 mRNA和蛋白表达，为STAT3基因靶向治疗提供一定的实验依据。     

徐元庚老中医治疗肛肠疾病学术经验简介

徐元庚老中医是我院肛肠科奠基人之一,徐老年逾花甲,幼年攻读中医典籍,博采众长,徐老积40余年丰富临床经验对肛肠疾病治疗确有独到之处,发表论文40余篇,并编著《痔瘘疾病临床指南》一书。现就笔者往昔随师学习的点滴体会简述如下:1 熟读经旨,著书立说徐老出身于中医世家,幼承庭训,勤奋研读经典,深究其理,对《内经》、《伤寒》、《金匮》至今仍能背诵如流。在治学方面,主张志于医者,在熟读经典,而后逐步参悟经旨,阅读诸家医集,抱着实事求是的客观态度,掌握取长舍短,去粗存精的学习方法,以防食古不化,或断章取     

酒精胎骨混悬液注射移植促进骨愈合实验研究

用酒精兔胎骨混悬液为植骨材料，以兔双侧桡骨中段３ｍｍ宽骨缺损横断骨折及肱三头肌为动物模型，通过注射将骨植入实验侧骨折端及肌肉内，对侧注入等量生理盐水作自身对照。术后进行免疫学、放射学、组织学及生物力学检查，结果表明：植骨不引起明显免疫排斥反应；植骨侧新骨形成多，骨折愈合快，抗弯应力强度大；肌内诱导成骨明显。酒精胎骨混悬液注射移植是一种简而有效的植骨材料和方法。     

ATP和SNP控制性降压对血流动力学的影响

对比研究三磷酸腺苷（ＡＴＰ）和硝普钠（ＳＮＰ）控制性降压对氯胺酮麻醉犬血流动力学的影响。选择犬１４只，随机分为ＡＴＰ组和ＳＮＰ组，由股静脉持续输入ＡＴＰ和ＳＮＰ使ＭＡＰ降低３０％￣４０％，维持３０ｍｉｎ，分别于降压前、降压后１５ｍｉｎ、３０ｍｉｎ及停止降压后１５ｍｉｎ用Ｓｗａｎ－Ｇａｎｚ漂浮导管测定血流动力学指标。结果表明，ＡＴＰ、ＳＮＰ降压期间ＳＶＲ、ＬＶＳＷ显著降低，ＣＯ、ＰＣＷＰ、ＳＶ、ＲＶ