体外循环过程中引起血红蛋白尿的原因分析

目的：探讨体外循环(CPB)过程中引起的血红蛋白尿的原因。方法：观察200例体外循环手术患术中灌注流量、转流时间、血液稀释后红细胞压积(HCT)、吸引器的流量及氧合器种类和气血比等指标,分析各项指标时产生血液蛋白尿的影响。结果：200例患中产生血红蛋白有42例,发生率2l％。其中以转机时间大于3小时、HCT大于30％、吸引器流量大于1000ml,使用鼓泡肺、高气血比时产生血红蛋白尿的影响最大。结论：防止体外循环过程产生血红蛋白尿的是一个综合结果,应尽量缩短CPB时间,稀释后HCT20％左右,吸引器使用应注意随时调整流量,选用优质膜肺,在应用鼓泡式氧合器时,注意调节气血比值。     

氯化镉对人胚肺成纤维细胞的恶性转化作用

为研究氯化镉致人胚肺成纤维细胞的恶性转化作用,用染色体分析,＾３Ｈ－ＴｄＲ掺入实验和流式细胞术检测了转化细胞染色畸变,各细胞周期时相细胞的比例和ＤＮＡ合成情况。结果显示,氯化镉在０．００４μｍｏｌ／Ｌ、０．０２μｍｏｌ／Ｌ、０．０４μｍｏｌ／Ｌ３个实验浓度都可使人胚肺成纤维细胞生长排列紊乱。     

鲤鱼精巢DNA对自由基的清除作用

目的：研究鲤鱼精巢DNA对自由基的清除作用。方法：采用 化学发光法测定鲤鱼精巢DNA对Cu2 -Vit C-H2O2发光体系产生的·OH自由 基的清 除作用,采用分光光度法检测鲤鱼精巢DNA对大鼠腹腔多形核白细胞(PMNs)呼吸爆发产生O ÷2自由基的清除作用,采用电子自旋共振(ESR)法测定鲤鱼精巢DNA对DPPH有机自由基的 清 除作用。结果：鲤鱼精巢DNA使Cu2 -Vit C-H2O2发光体系的发光计数率降低, 使 PMNs呼吸爆发产生O÷2自由基的数量减少,使DPPH的特征波峰逐渐降低直至消失。结论 ：鲤鱼精巢DNA对·OH自由基、O÷2自由基和有机自由基均有显著的消除作用。     

反相HPLC法测定妇康栓中没食子酸的含量

采用反相 HPL C法测定妇康栓中没食子酸的含量 :以对氨基苯甲酸为内标 ,检测波长为 2 73nm,以Hypersil BDS C18为固定相 ,甲醇 -四氢呋喃 -冰醋酸 -水 ( 0 .0 8∶ 0 .64∶ 2∶ 97.2 8,v/v)为流动相 ,平均回收率99.97% ,RSD 0 .73%。     

四种中药对血小板聚集性的影响

以 ADP、胶原为诱导剂研究清半夏、羌活、天冬、苦楝皮对血小板聚集性的影响。结果显示 :四种中药均具有抑制血小板聚集作用。以 IC50 为据分析 ,以 ADP为诱导剂抑制血小板聚集的强弱依次为 :羌活 >清半夏 >天冬 >苦楝皮 ;以胶原为诱导剂 :清半夏 >羌活 >天冬 >苦楝皮 ;而且 4种中药抑制血小板聚集作用与其药物的浓度的相关性良好。     

CT仿真胃内窥镜的临床应用研究

目的　研究CT仿真胃内窥镜 (computedtomographicvirtualgastroscopy ,CTVG)在胃部病变诊断中的应用价值、检查方法和镜下所见。方法　对 6 9例可疑胃部病变患者行胃不同气量充气后螺旋CT扫描 (准直器宽度 3mm ,螺距 1 2～ 2 5 ,扫描速度 0 8s/ 36 0°) ,以获得容积扫描数据 ,将原始数据进行重叠重建 (重建率 33%～ 6 7% ) ,再利用Navigator软件生成仿真胃内窥镜影像。结果　CTVG的准确性为 92 8% ,敏感性为 96 4% ,特异性为 78 6 %。CTVG对病变形态的显示与纤维胃镜 (fibrousgastroscopy,FG)检查及标本显示具有良好的对应性。CTVG在一定程度上具有显示慢性萎缩性胃炎、慢性糜烂性胃炎、慢性增殖性胃炎及急性出血性胃炎等细微病变结构的能力。当准直器宽度 3mm、螺距 1 2～ 1 5、重叠重建率 5 0 %～ 6 7%、呼吸控制良好、胃气量充足 ,以及采用适宜的扫描体位时可获得优质CTVG影像。结论　CTVG作为一种新兴的检查手段 ,有较高的临床应用价值     

HLAⅡ类抗原与病毒性心肌炎相关性的研究

目的：探讨人白细胞Ⅱ类抗原（ＨＬＡⅡ）与病毒性心肌炎（ＶＭＣ）的相关性。方法：用ＨＬＡ单克隆抗性体以微量淋巴细胞毒性试验调查３２例福建地区ＶＭＣ患者ＨＬＡ－ＤＲ和ＤＱ位点共２０个特异性抗原频率,并与４３例本地区政党人加以对照。结果：ＶＭＣ病人与ＨＬＡ－ＤＱ７（ＲＲ＝７．６００,ＰＣ＝０．００２）和ＤＱ２（ＲＲ＝０．１６１,ＰＣ＝０．００２）抗原分别为显著正、负相关结论：ＶＭＣ与ＨＬＡⅡ类抗原异常表     

缺血再灌注肺脏超微结构变化的电镜观察

目的　观察研究新西兰兔肺缺血 再灌注 (ischemic-reperfusion ,I R)过程肺毛细血管及肺泡上皮细胞超微结构变化。方法　取用新西兰兔I R模型的肺组织 (正常组、缺血 1h、再灌注 0 5h和再灌注 2h ,共 4组 )制作常规电镜标本 ,在透射电镜下进行观察。结果　缺血 1h肺毛细血管和肺泡上皮开始发生病理学变化 ,缺血后再灌注 0 5h时 ,结构明显损害 ,再灌注 2h后 ,毛细血管与肺泡结构均开始表现出损伤的修复趋势。结论　肺I R状态下 ,毛细血管内皮细胞和肺泡上皮细胞形态结构上的异常是导致肺功能障碍的病理学依据。     

磁性分离酶标技术在生殖内泌激素检测中的应用

目的　磁性分离酶标技术在生殖内泌激素检测中应用。方法　利用Serozyme -Ⅱ型磁性分离酶标仪及其配套试剂检测了六种生殖内分泌激素 (E2 、LH、FSH、HCG、T、PRL) ,并对其方法进行了评价。　结果　此法批内变异系数小于 4.1% ,批间变异系数小于 7.2 % ;标准曲线一次定标后至少可使用一个月。线性范围宽 (E2 在 0～ 11nmol/L、LH在 0～ 2 0 0miu/ml、FSH在 0～ 15 0miu/ml、HCG在 0～5 0 0miu/ml、T在 0 .5～ 5 5nmol/L、PRL在 0～ 2 5 0ng/ml) ;在 4h内显色稳定 ;回收率分别为 97.5 %、10 3%、97.4%、97.1%、96 .7%、97.2 % ,溶血对检测值有较大影响。仪器采用三波长同时检测 ,可消除试管因素的误差 ,可增大仪器量程 5倍。　结论　本法操作简单快速 ,且无放射性污染 ,对实验室要求低 ,有推广应用价值。     

Association of Periaortic Fat and Abdominal Visceral Fat with Coronary Artery Atherosclerosis in Chinese Middle Aged and Elderly Patients Undergoing Computed Tomography Coronary Angiography

Background and Aims:Coronary artery disease (CAD) is usually caused by atherosclerosis, which is associated with general obesity and stronger associations with localized ectopic fat depots have been reported. We measured body ectopic fat distribution in Chinese patients to determine the association with coronary artery atherosclerosis (CA).Methods:Patients undergoing coronary computed tomography angiography (CCTA) who agreed to participate in the study (n = 750, 50.4% men, mean age 64.8 years) had cardiovascular disease and risk assessment. Body ectopic fat depots were measured from CT and their association with CA, determined from CCTA, was evaluated by univariate and multivariate logistic regression models.Results:CAD with CA (CAD-CA) was present in 57.2% of participants with CAD of moderate/severe CA (CAD-msCA) present in 23.5% and both were significantly more frequent in men than in women. Overall, men had greater body mass index (BMI) but there was no difference in waist circumference (WC) between genders. However, significantly higher visceral adipose tissue (VAT) and periaortic fat volume (PAFV) were observed in men, whereas women had significantly higher abdominal subcutaneous adipose tissue (SAT). With increasing age, there was a significant decline in BMI, WC and SAT in men, but a significant increase of WC and VAT, PAFV and epicardial fat volume (EFV) in women. A high proportion of non-calcified plaques was observed in CAD-CA, 55.3% in CAD of minimal/mild CA (CAD-mmCA) with 38.7% exclusively non-calcified plaques, and 59.7% in CAD-msCA with multiple type plaques containing non-calcified ones. Multivariate logistic regression showed a significant association of PAFV with CAD-CA and CAD-msCA that was independent of general obesity and clinical risk factors, and independent of abdominal obesity in the highest PAFV quartile patients. VATA was associated with an increased prevalence of CAD-msCA in the patients in the upper 2 VATA quartiles that was independent of clinical risk factors and both general and abdominal obesity.Conclusions:We found age and gender differences of body ectopic fat distribution in Chinese patients with higher VAT and PAFV in men and higher SAT in women. With increased age, there was a decline of WC and SAT in men but not in women and an increase in WC, VAT and PAFV in women but not in men. PAFV was significantly associated with overall CAD-CA and CAD-msCA, while VAT was associated with CAD-msCA.