镉对大鼠肠粘膜中三价运铁蛋白形成的影响

通过经口投给100ppm氯化镉与不投给镉的两组Wistar雄性大鼠研究镉对大鼠肠粘膜中三价运铁蛋白形成的影响。结果表明,镉可降低脏器中贮藏铁的含量,可阻止铁进入肠全粘膜中,镉引起动物铁不足与其枯膜中铁蛋白及其它未知蛋白结合的关系应给以重新评价,而在体外试验中,即使给于高浓度氯化镉,对前运铁蛋白转为运铁蛋白也仅仅表现轻微的抑制作用。     

阻塞性肺病呼气末肺残气量的CT评价

目的:探讨呼气末高分辨率CT(HRCT)对阻塞性肺病(OLD)患者的肺残气量的评估价值.方法:对有肺功能检测结果的13名健康志愿者与14例OLD患者行吸气与呼气末HRCT扫描,测量吸气与呼气状态下肺CT值,并目测呼气末HRCT扫描时肺残气量的范围.结果:所有的OLD与8名健康志愿者都有肺内空气残余,但OLD的范围明显增大.OLD患者在吸气与呼气时肺CT值变化及残气量分数与健康者明显不同,且与肺功能密切相关(P＜0.01).结论:呼气末HRCT可用于评估OLD患者肺残气量并可诊断这类疾病.肺CT值的测量与肺残气量的评估是两种有价值的分析方法.     

LIS、HIS无缝连接及检验样本条码化应用体会——以海河医院为例

通过HIS系统升级增加医生工作站、LIS系统建设、LIS与HIS无缝连接、条码技术在LIS中的应用,实现病人基本信息及检验结果信息全医院联网.实践证明,该方案切实可行,可大大优化和规范医院工作流程,降低出错率,医患共同受益.     

体外厌氧条件下载铜蒙脱石杀菌效果的研究

载铜蒙脱石的杀菌作用通过脑心浸液肉汤 (BHI)二倍系列稀释法来判定其最小抑菌浓度 (MIC)。释放入肉汤和生理盐水中Cu2 的量通过原子吸收光谱仪测定。杀菌动力学研究采用改良的振荡瓶法。结果表明 :厌氧条件下 ,载铜蒙脱石具有很强的杀菌性能。载铜蒙脱石对放线共生放线杆菌的最小抑菌浓度为 2 .5 6mg/ml;对血液链球菌为 5 .12mg/ml。测得肉汤二倍稀释的载铜蒙脱石释放出的Cu2 量在 2 .39～ 38.6 5 ppm之间 ,在生理盐水中释放的Cu2 量为 1.85～ 15 .82ppm。本试验结果表明 ,蒙脱石无抗菌性能。     

HIV-2 DNA疫苗免疫小鼠的免疫反应观察

目的：用构建的HIV－2外膜蛋白gp105和核心蛋白gag基因的DNA疫苗免疫小鼠，评价其免疫效果。方法：将HIV－2型gp 105和gag基因克隆到表达载体pIRES1neo中，构建重组DNA疫苗质粒。间接免疫荧光试验证明，构建的DNA疫苗在真核细胞中表达了gp105或／和gag.构建的疫苗免疫小鼠后，用流式细胞仪测定CD4^ 、CD8^ T淋巴细胞亚类数，并用HIV－2抗体ELISA检测试剂盒检测免疫鼠血清中抗HIV－2抗体水平。结果：构建了3种HIV－2 DNA疫苗pIRES1gag、pIRSE1gp105和pIRES1gag-gp105，转染BHK细胞后均能表达抗原蛋白，免疫小鼠后可有效地刺激淋巴细胞增殖并诱导产生抗HIV－2特异性抗体，其中pIRES1gag-gp105免疫鼠中，淋巴细胞增殖最显著，而pIRES1gp105免疫鼠中HIV－2特异性抗体水平最高。结论：构建的DNA疫苗均能诱导机体产生免疫反应，其中pIRES1gp105诱导的体液免疫应答最显著，而pIRES1gag-gp105 诱导的细胞免疫响应最显著。     

A function of Fas-associated death domain protein in cell cycle progression localized to a single amino acid at its C-terminal region

FADD is an adaptor known to transmit apoptotic signals from members of the tumor necrosis factor receptor family. We show here that FADD has a domain implicated in cell proliferation. Mice bearing the Asp mutation in the serine 191 phosphorylation site are runted and anemic and display splenomegaly. Apoptosis is unimpaired in these mice, but they exhibit many immune developmental problems indicative of proliferative defects. Mutant FADD T cells are defective in cell cycle progression, suggesting that regulation of phosphorylation at serine 191 is essential for growth/proliferation. Remarkably, serine 191 is conserved among mammalian FADD proteins, but this C-terminal region is absent in lower organisms, suggesting that FADD acquired a domain during evolution, rendering it a "proliferation-apoptosis coupler" that balances cell proliferation and apoptosis.     

Prolongation of heart graft survival and spleen suppressor cell activity after donor specific blood transfusions in rats differing across the MHC

The aim of the study was the prolongation of heart allograft survival in rats after DST (donor specific blood transfusion), the characterization of T and B lymphocyte phenotypes in peripheral blood, spleen and lymph nodes and the evaluation of specific and nonspecific suppressor cell activity of spleen and blood lymphocytes of DST rats. Pretreatment of Wistar recipients with one, two and three doses of DST-s prolonged the heterotopic August graft survival to 11.0, 12.3 and 11.4 days, respectively (rats differed across the MHC). Spleen lymphocytes of transfused rats showed significant nonspecific suppressive activity in culture with syngeneic spleen lymphocytes of nontransfused rats stimulated with PHA, but not in culture with blood lymphocytes. Blood lymphocytes of transfused rats did not show any nonspecific suppressive activity. Spleen and blood lymphocytes of transfused rats did not demonstrate any specific suppressive activity in allogeneic MLC. The ratio of W3/25+/OX8+ (Th+/Tsup/cyt+) cells in peripheral blood was found increased in DST rats due to the decrease in the percentage of OX8+ cells whereas the opposite effect was observed with spleen cells (increase in the percentage of OX8+ cells after one DST). The number of OX6+ (Ia positive) cells and B cells in all transfused rats was found unchanged comparing with untreated animals.     

阑尾粘液囊肿的超声诊断

目的　探讨阑尾粘液囊肿 (AMC)的声像图表现 ,评价其临床意义 ;方法　回顾性分析了 7例AMC的声像图表现及超声与病理诊断的符合情况 ;结果　 6例诊断正确 ,1例误诊 ,符合率 85 7% ,本组病理声像图的主要表现有①圆形或卵圆形边界清楚的超声暗区 ;②AMC最小 37mm× 30mm ,最大 5 8mm× 6 5mm ;③位于右下腹相当于阑尾的解剖部位 ;结论　作者初步经验表明超声检查为发现AMC的一种敏感和可靠的方法 ,有助于对是否需行手术治疗作出正确的判断     

从人分离出两株甲型流感H9N2亚型毒株内部基因特性的研究

目的 了解2株从人分离出的H9N2亚型毒株内部基因特性，并弄清其来源。方法 用RT-PCR扩增目的基因，用P^CEM-T Vector(美国Promega公司)，4℃过夜连接，重组质粒转入dH5a细菌，筛选阳性菌落，酶切鉴定，送六合通公司自动测序。然后进行进化树分析。结果 2株测定毒株内部基因均为G9基因系，它们相互间除PA基因有差异外，其余5个基因均相同。结论 2株测定毒株的基因组均为G9基因系，它们是由携带不同基因特性H9N2毒株的禽群分别直接感染人，而不是来自同一禽的H9N2亚型流感病毒。     

冠心病家族史青少年载脂蛋白E、B的基因多态性

目的 探讨青少年载脂蛋白E(apolipoprotein E，apoE)、apoB基因多态性对冠心病的遗传易感性。方法 应用聚合酶链反应—限制性片段长度多态性技术，对244名健康汉族大学生(冠心病家族史阳性者109人，阴性者135人)的apoE、apoB XbaI、apoB 3’可变数目串联重复序列(variable number of tandem repeat ，VNTR)基因型进行分析。结果 阳性组的e4、x^ 、VNTR—B(hypervariable element，HVE＞38)等位基因频率显著高于阴性组(P＜0．05)，且与血总胆固醇、低密度脂蛋白—胆固醇、aPoBl00水平升高有显著相关(P＜0．05)。结论 apoE的e4、apoB Xba I的x^ 、apoB3’VNTR的VNTR—B可能为冠心病的重要遗传标记。