全文获取类型
收费全文 | 458篇 |
免费 | 49篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 3篇 |
妇产科学 | 13篇 |
基础医学 | 50篇 |
临床医学 | 24篇 |
内科学 | 89篇 |
皮肤病学 | 4篇 |
神经病学 | 37篇 |
特种医学 | 4篇 |
外科学 | 150篇 |
综合类 | 7篇 |
预防医学 | 77篇 |
眼科学 | 3篇 |
药学 | 33篇 |
中国医学 | 1篇 |
肿瘤学 | 8篇 |
出版年
2022年 | 4篇 |
2021年 | 8篇 |
2020年 | 9篇 |
2019年 | 10篇 |
2018年 | 11篇 |
2017年 | 9篇 |
2016年 | 8篇 |
2015年 | 5篇 |
2014年 | 14篇 |
2013年 | 15篇 |
2012年 | 25篇 |
2011年 | 29篇 |
2010年 | 16篇 |
2009年 | 14篇 |
2008年 | 15篇 |
2007年 | 23篇 |
2006年 | 12篇 |
2005年 | 17篇 |
2004年 | 15篇 |
2003年 | 8篇 |
2002年 | 6篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 6篇 |
1990年 | 5篇 |
1989年 | 8篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1986年 | 12篇 |
1985年 | 8篇 |
1984年 | 8篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1981年 | 8篇 |
1980年 | 7篇 |
1979年 | 9篇 |
1978年 | 8篇 |
1977年 | 8篇 |
1976年 | 9篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1973年 | 11篇 |
1972年 | 8篇 |
1971年 | 10篇 |
1970年 | 10篇 |
1969年 | 9篇 |
1968年 | 11篇 |
1967年 | 10篇 |
1966年 | 9篇 |
1965年 | 3篇 |
排序方式: 共有507条查询结果,搜索用时 15 毫秒
501.
502.
Karine Delgado Souza Ellenn Pollyanna Alexandre Fernandes Amanda Gubert Alves dos Santos Lainy Leiny de Lima Wagner Francisco Kennerly Marcondes Gonzaga Patricia Xander Gessilda de Alcantara Nogueira‐Melo Dbora de Mello Gonales Sant'Ana 《Parasite immunology》2019,41(9)
Evaluating the histopathological and morphometric changes caused by Leishmania (Leishmania) infantum chagasi infection either in the presence or absence of B‐1 cells. Wild‐type Balb/c and XID mice were used. Half of XID mice received B‐1 cells adoptive transfer (XID + B1). Five animals from each group were infected (Balb/c I, XID I and XID + B1 I), totalizing six groups (n = 5). After 45 days of infection, the ileum was collected for histological processing and analysis. After infection, the XID animals showed an increase in the thickness of the intestinal layers, in the depth and width of the crypt and in the villi width. However, the Balb/c I group showed a reduction in almost all these parameters, whereas the villi width was increased. The villi height decreased in the infected XID animals; however, it was increased in the XID + B1 I group. Leishmania (L) infantum chagasiinfection caused a decrease in the number of Paneth cells; however, their area was increased. Finally, goblet cells and enterocytes presented different change profiles among groups. This study showed that the parasite infection causes structural and histopathological alterations in the intestine. These changes might be influenced by the absence of B‐1 cells. 相似文献
503.
Momm F Jooss D Xander CJ Adebahr S Duncker-Rohr V Heinemann F Kirste S Messmer MB Grosu AL Becker G 《Strahlentherapie und Onkologie》2011,187(11):750-756
Introduction and Background
In the context of quality assurance, increasing demands are placed on the whole radiotherapy treatment process. The patients directly concerned generally do not realize most aspects of the quality assurance program (e.g., additional safety checks) during their daily therapy. It was the aim of this study to systematically ask patients about potential improvements during the course of radiotherapy treatment from their own perspective.Patients and Methods
In the defined time span (1 month), 624 radiotherapy patients (600 questionnaires were returned, 96.2%) were interviewed using a questionnaire newly developed to inquire about several aspects of their treatment. Furthermore, they were asked for their specific needs and suggestions for improvements that could be made during the course of radiotherapy treatment.Results
Overall, the patients were satisfied with the course of their radiotherapy treatment and with patient care. As an example, about 90% agreed with the statement: ??My first contact with the radiation oncology unit proceeded with kindness and competence so that I was given the impression that I will be well cared for in this clinic.?? Considering the organization of the course of radiotherapy, a large majority of patients attached great value to set appointments for the therapy fractions. A main point of criticism was waiting times or delays caused by servicing or machine failures. Small, low cost improvements as music in the therapy room were considered as important as expensive measures (e.g., daylight in the therapy room). The patients emphasized the importance of staff friendliness.Conclusion
The situation of radiotherapy patients was, in general, satisfactory. Future improvements can be mainly expected from smooth organisation of both planning and treatment which can be achieved by electronic scheduling systems. Many results of the survey could be easily implemented in daily practice. In matters of organization radiation oncology with its complex procedures can be used as a model for other clinical departments. 相似文献504.
Ryanodine receptor (RyR2) dysfunction, which may result from a variety of mechanisms, has been implicated in the pathogenesis of cardiac arrhythmias and heart failure. In this review, we discuss the important role of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in the regulation of RyR2-mediated Ca(2+) release. In particular, we examine how pathological activation of CaMKII can lead to an increased risk of sudden arrhythmic death. Finally, we discuss how reduction of CaMKII-mediated RyR2 hyperactivity might reduce the risk of arrhythmias and may serve as a rationale for future pharmacotherapeutic approaches. 相似文献
505.
Kranenburg LW Zuidema WC Weimar W Hilhorst MT Ijzermans JN Passchier J Busschbach JJ 《Transplantation》2007,84(8):965-971
BACKGROUND: This is the first large-scale interview study carried out in patients and potential donors who seem unwilling or unable to pursue living kidney donation. By investigating these groups, we explored whether further expansion of the living kidney donation program is feasible. METHODS: We interviewed 91 patients on the waiting list for a kidney transplant who did not pursue living kidney donation and their potential donors (n=53). We also included a comparison group. All respondents underwent an in-depth interview by a psychologist about topics that could influence their willingness to pursue living kidney donation. RESULTS: A total of 78% of the patients on the waiting list were willing to accept the offer of a living donor. The main reason for not pursuing living kidney donation was reluctance to discuss the issue with the potential donors. This was also found in the comparison group. Both groups indicated that if there was no donor offer, they tended to interpret this as a refusal to donate. This interpretation not always holds: more than one third (19 of 53) of the potential donors were open to consider themselves as a potential donor. On the other hand, a comparably sized group of potential donors (21 of 53) was reluctant about donation. The main reason for donor reluctance was fear for their health after donation. CONCLUSION: The majority of patients on the waiting list are willing to accept a living kidney donor, but adopt an awaiting attitude towards their potential donors. Offering those patients professional assistance should be considered. 相似文献
506.
siRNA injection induces sequence-independent protection in Penaeus monodon against white spot syndrome virus 总被引:9,自引:0,他引:9
White spot syndrome virus (WSSV) is a major disease in crustaceans, particularly shrimp, due to the current intensity of aquaculture practices. Novel strategies including vaccination to control this virus would be highly desirable. However, invertebrates lack a true adaptive immune response system and seem to rely on various innate immune responses. An alternative and more specific approach to counteract WSSV infections in shrimp could be by the exploitation of RNA interference. As long dsRNA molecules induce a general, sequence-independent anti-viral immunity in shrimp [Robalino, J., Browdy, C.L., Prior, S., Metz, A., Parnell, P., Gross, P., Warr, G., 2004. J. Virol. 78, 10442-10448], it was investigated whether shorter 21 nt siRNAs with homology to the WSSV vp15 and vp28 genes would give a sequence-specific interference response in the shrimp Penaeus monodon. Vp28 siRNAs as well as nonspecific control gfp siRNAs were able to specifically and efficiently silence their homologous genes in a heterologous baculovirus insect cell expression system. However, in shrimps no such a specific effect was observed. Shrimp injected with vp15 or vp28 siRNAs before WSSV challenge gave a significantly lower mortality rate, but not significantly different when shrimps were injected with gfp siRNA. Thus, large dsRNA molecules as well as siRNAs induce a sequence-independent anti-viral immunity when injected in shrimp. 相似文献
507.
Hydrogels are an important class of biomaterials that have the potential to be used as three-dimensional tissue engineering scaffolds for regenerative medicine. This is especially true in the central nervous system, where neurons do not have the ability to regenerate due to the prohibitory local environment following injury. Hydrogels can fill an injury site, replacing the growth-prohibiting environment with a more growth-permissive one. In this study, dextran and chitosan were incorporated into a methylcellulose and agarose hydrogel blend. This created several thermally sensitive polysaccharide hydrogel blends that had tunable mechanical and surface charge properties. Cortical neurons were cultured on the hydrogels to determine the blend that had the greatest neuron compatibility. Our results show that softer, more positively charged polysaccharide hydrogel blends allow for greater neuron attachment and neurite extension, showing their promise as CNS regeneration scaffolds. 相似文献