血浆miR-499在脓毒症合并心肌损伤的诊断价值

【目的】探讨血浆mi R-499水平在脓毒症合并心肌损伤的诊断价值。【方法】选择我院收治的脓毒症患者112例,根据患者有无合并心肌损伤分为脓毒症心肌损伤组(A组,n=45)、脓毒症未合并心肌损伤组(B组,n=67)。采用实时荧光定量技术检测患者血浆mi R-499水平。同期20例健康志愿者为对照(NC组)。【结果】脓毒症心肌损伤组mi R-499水平明显高于脓毒症未合并心肌损伤组及正常对照组(P<0.01);脓毒症合并重度心肌损伤患者(LVEF<30%)血浆mi R-499表达水平高于脓毒症合并轻度心肌损伤患者(30%≤LVEF<50%)(P<0.05);用ROC曲线分析mi R-499在脓毒症合并心肌损伤中的诊断价值,得到ROC曲线下面积为0.889(95%CI 0.821~0.957)。【结论】脓毒症心肌损伤患者外周血mi R-499表达水平显著升高,且和心肌损伤程度正相关,提示mi R-499的检测可作为脓毒症心肌损伤的判断指标。     

荔枝核皂苷和荔枝核黄酮对HepG2细胞胰岛素抵抗作用研究

目的 探讨荔枝核皂苷、荔枝核黄酮对胰岛素抵抗HepG2细胞糖代谢的影响。 方法 采用高浓度胰岛素培养基诱导HepG2细胞形成胰岛素抵抗模型,葡萄糖氧化酶法测定对照组、荔枝核皂苷组、荔枝核黄酮组、罗格列酮组细胞培养上清液中的葡萄糖含量,观察荔枝核皂苷、荔枝核黄酮对HepG2细胞胰岛素抵抗的作用。 结果 HepG2细胞在10^-6mol/L的胰岛素中作用24 h,细胞对胰岛素的抵抗作用最为明显。以此条件构建胰岛素抵抗细胞模型,对该模型给予荔枝核皂苷、荔枝核黄酮干预后,细胞培养上清液中葡葡糖含量未能显著降低。 结论 本研究提示荔枝核皂苷、荔枝核黄酮不能有效改善胰岛素抵抗。     

重楼活性单体PP-22对人结肠癌SW620细胞增殖和凋亡的影响

目的 探讨重楼活性单体PP-22对人结肠癌SW620细胞增殖和凋亡的影响及其联合5-氟尿嘧啶(5-FU)和洛铂的抗肿瘤效果。方法 采用噻唑蓝(MTT)法和克隆形成抑制实验观察不同浓度PP-22对人结肠癌SW620细胞增殖的抑制作用;分别观察PP-22联合5-FU和洛铂对人结肠癌SW620细胞增殖的抑制作用;碘化丙锭单染流式细胞术检测细胞周期变化及细胞凋亡水平;Western blot法检测PP-22作用后细胞凋亡相关蛋白的表达。结果 重楼单体PP-22能显著抑制SW620细胞的生长,作用呈剂量-效应关系;随着PP-22浓度的增加,细胞克隆形成逐渐减少,与细胞对照组和DMSO组相比有显著差异;PP-22联合不同浓度的5-FU和洛铂作用于SW620细胞48 h,可提高SW620细胞对5-FU和洛铂的敏感性;不同浓度PP-22作用于SW620细胞后,细胞周期阻滞于S期;PP-22作用后存在Caspase-3和Caspase-9蛋白的活化和降解,抗凋亡蛋白Bcl-2、Bcl-xL减少,促凋亡蛋白Bax的表达增加。结论 PP-22能显著抑制人结肠癌SW620细胞增殖,诱导细胞凋亡,提高SW620细胞对5-FU和洛铂的敏感性。     

PPARγ在子宫内膜异位症中的作用研究进展

现有的研究已阐明激活PPARγ具有抑制炎症反应、抗血管生成等作用,而炎症反应和血管生成均参与了子宫内膜异位症的发生发展,并且PPARγ在正常子宫内膜组织及异位子宫内膜组织均有表达。PPARγ在子宫内膜异位症中的作用受到越来越多的关注,该文就PPARγ在子宫内膜异位症中的意义作一综述。     

NO调节在小鼠生发泡期卵母细胞体外成熟中的作用

目的观察不同浓度硝普钠(SNP)、左旋硝基精氨酸甲基酯(L-NAME)对小鼠生发泡(GV)期卵母细胞体外成熟的影响。方法给小鼠腹腔注射PMSG 8IU,取其双侧卵巢,用卵母细胞体外培养法,分为L-NAME组、SNP+dbc AMP+L-NAME组、P+dbc AMP+SNP+L-NAME组,在倒置生物显微镜下观察卵母细胞生发泡破裂(GVBD)率及第一极体(PB1)排出率。结果 SNP+dbc AMP组的GVBD率与PB1率分别为(52.5±0.4与32.5±0.1)显著高于dbc AMP组的(28.0±0.2与11.1±0.7)(P0.05)。P+dbc AMP+SNP+L-NAME组的GVBD率与PB1率分别为(62.8±0.3与20±1.4)显著低于P+dbc AMP+SNP组的(90.1±1.3与66.6±0.9),GVBD率与PB1率显著性降低(P0.05)。结论 L-NAME与二丁酰环腺苷酸(dbc AMP)抑制小鼠卵母细胞成熟时,小剂量NO呈现促进作用,这种促进作用在孕酮(P)存在时进一步加强。     

黄芪对缺氧缺血性脑损伤新生鼠脑组织Nogo-A蛋白表达的作用及意义

目的探讨Nogo-A蛋白在缺氧缺血性脑损伤新生鼠脑组织中的表达特点及黄芪对其表达的影响方法 7日龄Wistar新生大鼠72只,清洁级,雌雄不限,平均体重10-16g,随机分为假手术组,HIBD组,黄芪治疗组,每组24只。分离左侧颈总动脉并结扎,吸入92%N2+8%O2氮氧混合气体行缺氧处理2h,制成HIBD模型。假手术组只作分离左侧颈总动脉而不结扎,不作缺氧处理,不给与药物处理。黄芪治疗组于建模成功后立即及3h腹腔注射0.5ml/只,假手术组及HIBD组大鼠腹腔注射等量生理盐水,上述各组均于术后6h、12h、24h、72h随机抽取6只,于麻醉下处死取脑组织,HE染色观察脑组织病例变化,免疫组化法检测Nogo-A蛋白表达情况。结果与假手术组相比,HIBD组术后脑组织Nogo-A蛋白表达增高,但Nogo-A蛋白随各时间点有先上升后下降的趋势,差异有统计学意义(P0.05),与HIBD组相比,黄芪治疗组各时间点Nogo-A蛋白,差异有统计学意义(P0.05)。结论提示黄芪可下调缺氧缺血性损伤大鼠脑组织Nogo-A蛋白的表达,对神经元和神经纤维具有保护作用。     

四川省色达县居民棘球蚴病防治知信行调查及影响因素分析

目的了解四川省色达县居民棘球蚴病防治知识、态度和行为现状及其影响因素,为当地进一步实施健康教育干预措施提供科学依据。方法 2018年9月,采用多阶段分层随机抽样方法,共抽取10个乡(镇),每个乡(镇)随机抽取2~3个行政村,每个村随机抽取20~40户,每户调查1~2名村民。采用入户问卷调查了解调查居民棘球蚴病防治知识、态度和行为情况,采用χ~2检验及logistic回归分析对影响居民防治知识合格率的因素进行分析。结果本次调查共发放问卷760份,回收有效问卷748份,问卷回收率为98.42%。色达县居民棘球蚴病防治知识合格率为56.42%(422/748),其中"人是怎样感染棘球蚴的?"知晓率仅为48.40%;调查对象棘球蚴病防治积极态度持有率在64.71%~94.79%,防治行为正确率在10.40%~82.45%。单因素分析表明,居民棘球蚴病防治知识合格率影响因素包括性别、职业、受教育程度、是否曾经接受棘球蚴病筛查服务及健康教育(P均<0.05);二分类logistic回归分析结果显示,女性、主要职业为牧民、小学及以下受教育程度、未接受过棘球蚴病筛查服务及健康教育是导致防治知识知晓率不合格的影响因素(P均<0.05)。结论四川省色达县居民棘球蚴病防治知识知晓率偏低、行为正确率不高,需因地制宜地采取多种形式加强疾病防治知识宣传,并进行适当行为干预。     

心脏术后急性肾功能衰竭的替代治疗

目的　评估肾脏替代治疗对心脏术后急性肾功能衰竭的效果。方法　 1995年 1月至 2 0 0 3年 7月 ,5 4例心脏术后因急性肾功能衰竭接受了肾脏替代治疗 ,其中腹膜透析 2 0例 ,血液透析 15例 ,连续性肾脏替代治疗 19例。结果　 14例患者肾功能恢复出院 ,6例病情好转后自动出院 ,34例死亡。结论　肾脏替代治疗是心脏术后急性肾功能衰竭的一种有效治疗手段 ,应尽早实施。     

Chemopreventive effects of rofecoxib and folic acid on gastric carcinogenesis induced by N‐methyl‐N′‐nitro‐N‐nitrosoguanidine in rats

OBJECTIVES: Epidemiological and experimental studies indicate that non‐steroidal anti‐inflammatory drugs (NSAIDs) are chemopreventive agents of gastrointestinal cancers, but few studies on gastric cancer have been carried out. A decrease in folic acid supplement and subsequent DNA hypomethylation are related to gastrointestinal cancers, and it has been shown that high‐dose folic acid may interfere with gastric carcinogenesis in dogs. The objective of this study was to investigate the effects of rofecoxib, a selective cyclooxygenase‐2 (COX‐2) inhibitor, and folic acid on the chemoprevention of gastric cancer induced by N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG) in Wistar rats, and to evaluate the cell proliferation of gastric mucosa in different experimental groups. METHODS: Eighty male Wistar rats were randomly divided into five groups (16 rats in each group). In the control group, the rats were given pure water and basal diet. In the MNNG group, the rats received MNNG in drinking water (100 mg/L) and basal diet. In the MNNG + low‐dose rofecoxib group, the rats were given MNNG and rofecoxib 5 mg/kg per day with basal diet. In the MNNG + high‐dose rofecoxib group, the rats were given MNNG and rofecoxib 15 mg/kg per day with basal diet. In the MNNG + folic acid group, the rats were given MNNG and folic acid 5 mg/kg per day with basal diet. The experiment was terminated at 50 weeks, and all rats were killed. Blood samples of 3 mL were obtained for measurement of serum folic acid concentrations in the control group, the MNNG group and the MNNG + folic acid group by using chemiluminescent method. The stomach was removed from all rats for histopathological examination and immunohistochemical study. Proliferating cell nuclear antigen (PCNA) expression in gastric epithelial cells was also determined. RESULTS: In the MNNG group, five of 11 rats (45.5%) developed gastric cancer, while in all other four groups no gastric cancer was found (P < 0.05). The positivity rate of PCNA expression in the cancerous tissues was significantly higher than that in the non‐cancerous tissues (80.0%vs 14.1%, P < 0.05). The positivity rate of PCNA expression in the gastric mucosal cells of the MNNG group was significantly higher than that in the other four groups. The mean serum folic acid concentration of rats was significantly higher in the MNNG + folic acid group (193.70 ± 60.73 ng/mL) than those in the control group (84.21 ± 25.26 ng/mL) and the MNNG group (72.27 ± 16.70 ng/mL, P < 0.05). It was shown that both low‐ and high‐dose rofecoxib as well as folic acid interfered with the development of gastric cancer induced by MNNG in Wistar rats. CONCLUSIONS: The results indicate that rofecoxib as well as folic acid interferes with gastric carcinogenesis induced by MNNG in Wistar rats, and the suppression of gastric cell proliferation may play a crucial role in the chemoprevention of gastric cancer by rofecoxib and folic acid. The higher serum folic acid concentration of rats may play an important role in the prevention of gastric cancer.