GP-084泡沫硅橡胶和SY-1硅橡胶之间粘结性能的测定

目的:评价GP-084泡沫硅橡胶和SY-1硅橡胶之间的粘结性能。方法:分别测试有无Adhesive-AGP-084泡沫硅橡胶和SY-1硅橡胶之间的粘结强度;并采用电镜观察2种材料之间的粘结界面。结果:使用Adhesive-AGP-084泡沫硅橡胶和SY-1硅橡胶之间的粘结强度为(1.31±0.09)MPa,无Adhesive-AGP-084泡沫硅橡胶和SY-1硅橡胶之间的粘结强度为(1.29±0.11)MPa,2组测试结果的差异无统计学意义;电镜观察2种材料之间没有明显界面,完全融合在一起。结论:GP-084泡沫硅橡胶和SY-1硅橡胶2种材料之间具有良好的粘结效果,可以完全融合在一起,无明显界面形成。     

模型转化法建立磨牙及其牙周组织的三维有限元模型

目的建立下颌第二恒磨牙及其牙周膜、牙槽骨的分析用模型。方法应用CAE软件3D MAX6.0及CATIA4.0生成磨牙及其牙周组织的三维有限元模型,利用HYPERMESH划分网格,生成可分析的三维有限元实体模型。结果建立了下颌第二恒磨牙及其牙周膜、牙槽骨的三维有限元模型,可以分别进行受力状态下应力、应变的分析。结论该模型可供口腔正畸矫治中磨牙的受力分析应用。     

童年期心理虐待和忽视、述情障碍对大学生无聊倾向的影响

目的探讨童年期心理虐待和忽视、述情障碍对大学生无聊倾向的影响及其关系。方法采用大学生无聊倾向问卷(boredom proneness scale for college students,BPS)、儿童心理虐待和忽视量表(child psychological abuse and neglect scale,CPANS)、多伦多述情障碍量表(the Toronto alexithymia scale,TAS-20)对1557名大学生进行调查,采用SPSS 22.0进行描述性统计、相关分析和回归分析。结果童年心理虐待[(26.540±8.985)分]、忽视[(32.665±10.651)分]、述情障碍的识别情感维度[(19.359±4.835)分]、描述情感维度[(14.173±3.001)分],外向思维维度[(20.383±3.429)分]与无聊倾向总分[(101.109±19.735)分]、外部刺激维度[(66.022±21.079)分]存在正相关(r=0.160~0.559,均P<0.01),而与内部刺激维度[(35.132±8.207)分]存在负相关(r=-0.302~-0.104,均P<0.01)。分层回归分析显示,在控制性别和年龄的影响后,心理虐待与忽视是无聊倾向、内部刺激和外部刺激的影响因素,分别可解释总变异的14.3%,5.1%和18.2%(△F=130.561、41.968、172.711,均P<0.01)。述情障碍的识别情绪、描述情绪和外向思维是无聊倾向、内部刺激和外部刺激的影响因素,分别可解释总变异的17.5%,9.9%和25.5%(△F=134.274、60.696、234.383,P<0.01)。结论童年心理虐待和忽视、述情障碍对大学生无聊倾向存在显著影响,主要体现在对无聊倾向的外部刺激的影响上,其中述情障碍影响最大、解释力最高。     

Proteomics Study on the Differentially Expressed Proteins in 16HBE Cells Exposed to Beryllium Sulfate

Beryllium (Be) is a non-radioactive element with carcinogenic properties, and presents serious occupational and environmental hazards from its resulting toxicants, which could significantly impact the health of the occupational population[1-3]. Beryllium and its compounds can cause diseases such as acute chemical pneumonia, lung cancer, and chronic beryllium disease, which predominantly occurs alongside either lung granuloma or pulmonary fibrosis[4,5]. Furthermore, the mechanism by which beryllium is toxic has not been fully elucidated; therefore, studies on its health hazards as well of its compounds are important. An in-depth study of the mechanism of beryllium toxicity is especially essential to prevent and control its associated health hazards. Tandem mass tag (TMT) technology is a reliable technology in quantitative proteomics, which can be implemented to perform relative quantification and identification analysis of proteins, peptides, nucleic acids, and other biological macromolecules.     

Mash1 efficiently reprograms rat astrocytes into neurons简

To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mashl and Brn2 have been previously shown to cooperate to reprogram fibroblasts into neurons. In this study, we examined astrocytes from 2-month-old Sprague-Dawley rats, and found that Brn2 was expressed, but Mashl was not detectable. Thus, we hypothesized that Mashl alone could be used to reprogram astrocytes into neurons. We transfected a recombinant MSCV-MASH1 plasmid into astrocytes for 72 hours, and saw that all cells expressed Mashl. One week later, we observed the changes in morphology of astrocytes, which showed typical neuro- nal characteristics. Moreover, β-tubulin expression levels were significantly higher in astrocytes expressing Mashl than in control cells. These results indicate that Mashl alone can reprogram astrocytes into neurons.     

Development of a Valid Simplified Chinese Version of the Oxford Hip Score in Patients With Hip Osteoarthritis

Background

Although the Oxford Hip Score has been translated and validated in several languages, there is currently no Chinese version of the outcomes measurement. Our study aims to crossculturally adapt and validate the Oxford Hip Score into a simplified Chinese version.

Questions/purposes

We tested the (1) reliability; (2) validity; and (3) responsiveness of the Chinese version of the Oxford Hip Score.

Methods

First we translated the Oxford Hip Score into simplified Chinese, then back into English, then held a consensus meeting to achieve the final simplified Chinese version. Then we evaluated the psychometric properties of Chinese version of the Oxford Hip Score in patients undergoing total hip arthroplasty (THA). All patients undergoing THA between July and December 2012 were invited to participate in this study; a total of 108 (79% of 136 invited) did so. To assess the test-retest validity, all participants completed the Chinese version of the Oxford Hip Score again with a 2-week interval. Pearson correlation coefficient was used to evaluate the construct validity between the Chinese version of the Oxford Hip Score and visual analog scale (VAS), Harris hip score, and eight individual domains of the SF-36. Responsiveness was demonstrated by comparing the pre- and postoperative scores of the Chinese version of the Oxford Hip Score.

Results

The test-retest reliability with intraclass correlation coefficient (0.937) and internal consistency with Cronbach’s alpha (0.91) were excellent. The Chinese version of the Oxford Hip Score correlated with the Harris hip score (0.89, p < 0.01), VAS (−0.79, p < 0.01), and Physical Functioning (0.79, p < 0.01) and Bodily Pain (0.70, p < 0.01) domains of SF-36, which suggested construct validity. No floor or ceiling effects were found. The effect size and standardized response mean values were 3.52 and 3.31, respectively, indicating good responsiveness.

Conclusions

The Chinese version of the Oxford Hip Score showed good reliability, validity, and responsiveness in evaluating standard Chinese-speaking patients with hip osteoarthritis undergoing THA. It can be used by clinical surgeons as a complement to the traditional outcome measures.     

BMP signaling mediates astrocyte differentiation of oligodendrocyte progenitor cells

Oligodendrocyte precursor cells (OPCs) can differentiate into oligodendrocytes or astrocytes, depending on cellular microenvironments. OPCs, cultured in medium supplemented with 10% (v/v) fetal bovine serum (FBS), give rise to type II astrocytes that express glial fibrillary acidic protein and a cell surface ganglioside that is recognized by A2B5 monoclonal antibody. However, the factors in FBS that direct the astrocyte differentiation are not determined. Moreover, bone morphogenetic proteins (BMPs) have been reported to be involved in astrocyte differentiation of neural progenitor cells. We therefore examined whether BMPs are responsible for the serum-mediated astrocyte differentiation from OPCs. OPCs were isolated from the spinal cords of Wistar rat embryos (at day 14) using the A2B5 antibody. We measured the concentrations of BMP-2 and BMP-4 in FBS and rat and human sera and the expression of mRNAs for three types of BMP receptors (BMPRIa, Ib and II) in OPCs by RT-PCR. The serum samples of the three species contained BMP-2 and BMP-4, as judged by ELISA with each monoclonal antibody, and the BMP receptor mRNAs are expressed in OPCs. When OPCs were cultured in the medium containing 10% FBS, cells (more than 95%) differentiated into type II astrocytes. However, when OPCs were pretreated with noggin, a soluble antagonist of BMP action, the degree of astrocyte differentiation was markedly decreased from 95.39 to 38.36%. Taken together, these results suggest that BMP signaling may be responsible for the serum-mediated astrocyte differentiation of OPCs. Our findings provide new insights into the molecular basis of differentiation of OPCs.     

颈外动脉超选择栓塞在窦旁肿瘤手术中的应用

目的 探讨颈外动脉超选择栓塞在上矢状窦旁肿瘤手术中的应用价值.方法 回顾性分析45例上矢状窦旁肿瘤患者的临床资料,根据肿瘤大小及术前是否栓塞进行分组,对各组间术中出血量、手术时间、手术切除程度进行比较.结果 ＜4 cm的窦旁肿瘤,栓塞组与未栓塞组相比,平均失血量、手术全切率及手术时间差异均无统计学意义（P＞0.05）,但对于＞4 cm的肿瘤,栓塞组较未栓塞组手术术中失血明显减少（P＜0.01）,全切率高,手术时间短.结论 术前颈外动脉超选择栓塞能明显减少窦旁肿瘤术中出血,尤其是对较大肿瘤,术前颈外动脉超选择栓塞不但能明显减少术中出血,还能有效缩短手术时间,提高手术安全性及全切率.