Comparative analysis of transient receptor potential channel 5 opposite strand-induced gene expression patterns and protein-protein interactions in triple-negative breast cancer

In patients with triple-negative breast cancer (TNBC), high tumour mutation burden and aberrant oncogene expression profiles are some of the causes of poor prognosis. Therefore, it is necessary to identify aberrantly expressed oncogenes, since they have the potential to serve as therapeutic targets. Transient receptor potential channel 5 opposite strand (TRPC5OS) has been previously shown to function as a novel tumour inducer. However, the underlying mechanism of TRPC5OS function in TNBC remain to be elucidated. Therefore, in the present study TRPC5OS expression was first measured in tissue samples of patients with TNBC and a panel of breast cancer cell lines (ZR-75-1, MDA-MB-453, SK-BR-3, JIMT-1, BT474 and HCC1937) by using qRT-PCR and Western blotting. Subsequently, the possible effects of TRPC5OS on MDA-MB-231 cells proliferation were determined using Cell Counting Kit-8 and 5-Ethynyl-2′-deoxyuridine assays after Lentiviral transfection of MDA-MB-231. In addition, potential interaction partners of TRPC5OS were explored using liquid chromatography-mass spectrometry (LC-MS)/MS. Gene expression patterns following TRPC5OS overexpression were also detected in MDA-MB-231 cells by using High-throughput sequencing. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analysis were then used to systematically verify the potential interactions among the TRPC5OS-regulated genes. The potential relationship between TRPC5OS-interacting proteins and gene expression patterns were studied using Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) analysis. TRPC5OS expression was found to be significantly higher in TNBC tumour tissues and breast cancer cell lines compared with luminal tumour tissues and ZR-75-1. In addition, the overexpression of TRPC5OS significantly increased cell proliferation. High-throughput sequencing results revealed that 5,256 genes exhibited differential expression following TRPC5OS overexpression, including 3,269 upregulated genes and 1,987 downregulated genes. GO analysis results indicated that the functions of these differentially expressed genes were enriched in the categories of ‘cell division’ and ‘cell proliferation’ regulation. KEGG analysis showed that the TRPC5OS-regulated genes were associated with processes of ‘homologous recombination’ and ‘TNF signalling pathways’. Subsequently, 17 TRPC5OS-interacting proteins were found using LC-MS/MS and STRING analysis. The most important protein among interacting proteins was ENO1 which was associated with glycolysis and regulated proliferation of cancer. In summary, data from the present study suggest that TRPC5OS overexpression can increase TNBC cell proliferation and ENO1 may be a potential target protein mediated by TRPC5OS. Therefore, TRPC5OS may serve as a novel therapeutic target for TNBC.     

CSCO guidelines for colorectal cancer version 2022: Updates and discussions

<正>Colorectal cancer(CRC) is the third most common cancer worldwide and the second most common in tumor-related mortality by Global Cancer Statistics 2020(1). Recent data show that the incidence and mortality of CRC in China are increasing(2-4), with the number of new cases and deaths reaching 607,900 and 261,777 in 2019.     

优秀游泳运动员大运动量训练期间的超声心动图测定

<正> 自1967年Feignbaum首先应用超声心动图测算心搏量以来,发展迅速,目前已广泛用于心脏功能和结构的测定。Morganroth等1975年发表题为“有训练运动员运动性左室肥大”。我国白洁心等于1979年报道了运动员超声心动图的测量分析。近几年来,国内关于运动员的超声心动图已先后发表了二十多篇文章,都是属于一次性的测定,在作横向比较时,由于仪     

运动训练中服用“高效强力饮”对心血管功能的效应

本文研究了一种中药水剂——高效强力饮对44名中长跑和游泳运动员运动能力的影响。测试结果表明:服药组与对照组相比,心搏量、射血分数和△D%均有明显增高;而运动后血乳酸水平,平均动脉压和总外周阻力均明显下降。这些都表明服药组有氧代谢能力方面有改善。     

某院青壮年患者疾病谱构成分析

目的 探讨青壮年住院病例的特点.方法 对某院青壮年住院病案资料进行回顾性分析.结果 女性病人多于男性;前五位住院疾病为妊娠分娩产褥期病、消化系统病、肿瘤、泌尿生殖系统病和损伤中毒.前五位死因为肿瘤、循环系统病、损伤中毒、消化系统病和呼吸系统病.前后5年对比,循环系统病有增加趋势,损伤中毒有减少趋势.结论 把女性青壮年人群作为保健重点,并注重青壮年人群的恶性肿瘤、循环系统病和损伤中毒的防治,才能提高生命质量,降低病死率.     

化合物XW630对成骨细胞的作用

为探讨新合成化合物ＸＷ６３０对成骨细胞的作用，采用噻唑蓝比色法（ＭＴＴ）法和碱性磷酸酶（ＡＬＰ）测定法观察了ＸＷ６３０对体外培养的成熟大鼠头盖骨成骨细胞的增殖和ＡＬＰ的表达，并用抗酒石酸磷酸酶染色法观察ＸＷ６３０对体外培养的破骨细胞形成的影响。结果显示：ＸＷ６３０有刺激成骨细胞增殖，提高ＡＬＰ活性及抑制甲状旁腺素促进破骨细胞形成的作用，从而表明ＸＷ６３０有可能成为治疗骨质疏松症的有效药物。     

美满霉素等9种药物对人全血吞噬细胞和非细胞体系的抗活性氧作用

观察和比较美满霉素、四环素、灭滴灵、青霉胺、氯喹、布洛芬、头孢拉定、异搏定和三氟拉嗪对中性粒细胞（ＰＭＮ）产生的ＲＯＳ和非细胞体系产生的Ｏ和·ＯＨ的影响。应用化学发光法（ＣＬ）测定人全血吞噬细胞在受调理的酵母多糖诱导下产生的活性氧（ＲＯＳ）、黄嘌呤－黄嘌呤氧化酶体系产生的Ｏ和ＶｉｔＣ－ＣｕＳＯ４－酵母多糖－Ｈ２Ｏ２体系产生的·ＯＨ。结果：所有９种药物都具有抑制或清除中性粒细胞产生的ＲＯＳ；美满霉素、四环素、三氟拉嗪具有清除Ｏ作用；青霉胺和美满霉素则有清除·ＯＨ的作用。结论：美满霉素是９种药物中较为安全、有效的清除皮肤疾患中ＲＯＳ的首选药物。     

电力电缆的预防性试验

主要叙述了电缆做预防性试验及其试验项目、方法和要求，以及需注意的问题。     

初次全膝关节置换术后并发症及翻修手术的原因分析

目的 探讨初次全膝关节置换术后30 d内相关并发症发生情况及翻修手术的相关因素.方法 收集2001年1月至2012年12月在北京协和医院骨科进行初次全膝关节置换术患者的临床资料,假体均为固定平台假体,采用骨水泥固定,排除翻修病例及血友病关节炎患者.共有1 920例患者(2 779例次全膝关节置换手术)纳入研究,男性323例,女性1 607例;年龄25～86岁,平均(66±9)岁.骨关节炎1 720例(89.58％),类风湿关节炎168例(8.75％),强直性脊柱炎12例(0.63％),继发骨关节炎20例(1.04％).随访患者术后30 d内发生的主要系统并发症、局部并发症及发生的翻修手术及相关因素.结果 随访截至2013年12月,共有1 854例患者(2 693个关节)获得随访,失访率为3.44％.术后平均随访67个月,死亡3例.41例(2.21％)患者出现系统并发症,其中最常见的为呼吸系统并发症(0.49％,9/1 854)及心血管并发症(0.38％,7/1 854).术后经超声证实的症状性深静脉血栓形成发生率为3.02％ (56/1 854),其中7例发生肺栓塞.术后发生局部并发症24例(1.29％),包括伤口愈合不良、伤口感染、神经损伤.59个关节接受翻修手术治疗,常见原因包括感染后松动(1.19％,32/2 693)和术后关节僵硬(0.37％,10/2693).结论 初次全膝关节置换术后30 d内最常见系统并发症为呼吸系统及心血管系统并发症.感染后松动是术后翻修最常见的原因.