铍病免疫的实验研究之一——家兔染尘试验

本实验采用雄性健康家兔18只,分成两个实验组和1个对照组,每组6只。实验组家兔分别每月气管注入氧化铍8mg/kg、16mg/kg,连续两次;对照组注入生理盐水。结果两个实验组分别在染毒后4个月、3个月均形成了肺部肉芽肿病变。实验发现,免疫指标与X线表现及病理改变之间存在着一定的关系。免疫指标阳性反应在某种程度上能反映出病理组织学改变。给临床诊断铍病提供了部分实验依据。     

门诊老年冠心病患者用药知识需求的调查

冠心病是老年人的常见病、多发病,致残或死亡率已居常见病之首[1]。目前,药物治疗仍是冠心病二级预防的主要措施。近年来研究发现,83·3%的冠心病患者无法正确使用药物,用药依从性差[2]。分析原因,87%的门诊老年慢性病患者包括冠心病患者在内,主观不遵医用药是由于在用药过程中出现诸多用药问题时得不到相关知识信息支持所导致[3]。另外,通过调查证实冠心病患者对用药知识的需求亦居前列,尤其是出院后用药知识是首要需求[2,4-6]。由此表明,对门诊老年冠心病患者进行用药知识教育十分必要。依据健康教育学方法,分析患者的教育需求是制定教育…     

呼出气氢测定试验对飞行人员乳糖酶缺乏症的研究

对66名汉族飞行人员进行乳糖呼出气氢测定试验,乳糖吸收不良的发生率为83.3％,其中乳糖不耐受者占34.6％;与一般汉族人群无明显差别。对10名确定为中度以上乳糖吸收不良的飞行人员进行250ml鲜牛奶的试验结果,有50％呼出气氢含量在正常范围,并无一例出现胃肠道症状。提示较长期食用牛奶未能使乳糖酶缺乏状态发生改变,但每日食用适量牛奶属合理营养。     

Divergent expression and localization of aquaporin 5, an exocrine-type water channel,in the submandibular gland of Sprague-Dawley rats

By Western blot analysis, the expression level of aquaporin (AQP) 5 in the submandibular gland (SMG) was found to be different among individual rats of the Sprague-Dawley (SD) strain. Such differences were observed for AQP5 but not for AQP1 and consequently the SD strain was divided into two groups, one expressing a high level of AQP5 and the other a low one. The difference in average intensity of expression between the two groups was more than twofold. Immunohistochemical analysis of the SMG demonstrated that the AQP5 protein was localized in the basal and apical/lateral plasma membrane of acinar cells in rats expressing the high level of AQP5. In the rat expressing the low level, however, this channel protein was localized strongly in the apical/lateral plasma membrane, but only very weakly in the basal membrane of the acinar cells. Such a diverse localization of AQP5 was confirmed by Western blotting as well. Breeding between brother and sister was repeated for two times within high expressers and low expressers to obtain the third generation progenies (F2); the AQP5 level of the SMG in the third generation (F2 rats) from high expressers was significantly higher than the F2 from low expressers. Our present study suggests the existence of genetic variation in the expression of a water channel protein, AQP5, in rats.     

Radiology information systems,picture archiving and communication systems,teleradiology— Overview and design criteria

Information technology (IT), long taken for granted in commercial settings, is now being utilized for healthcare applications. Medical imaging has lagged comparatively due to the extremely vast data content of each frame; thus, the requirement for expensive high-end components. Further, IT in radiology has evolved from two distinctly separate camps—information systems, known as RIS (radiology information systems) and PACS (picture archiving and communications systems). Both RIS and PACS applications have migrated to the PC environment, enabling cost-effective implementation, but from two backgrounds: RIS from vendors using conventional information systems platforms and products, and PACS from radiographic film and modality vendors. The radiology department at Texas Tech University has assembled a seamlessly integrated, enterprise-wide RIS/PACS/teleradiology intranet. The design criteria include user-friendliness, flexibility to respond to changing needs, and open modular architecture to assure interoperability, cost-effectiveness, and future-proofing of investment. Since no single venor could provide an integrated system meeting our specifications, we decided to assume the burden of constructing our own system. As the system integrator, we embrace open architecture, thus enabling the incorporation of industry-standard-compliant, COTS (commercially off the shelf) products as modules. Microsoft Windows NT operating system, Visual C++ programming language, TCP/IP (transmission control protocol/internetworking protocol), relational SQL (structured query language) database, ODBC (open database connectivity), HL-7 (health level seven) and DICOM (digital imaging and communications in medicine) interfaces are utilized. The usage of COTS components reduces the cost to very affordable levels. With this approach, any module in our system can be replaced when outmoded, without affecting other modules in our system, making it truly future-proof. Construction and evolution of our system (TECHRAD) is reviewed.     

猕猴输精管内注射高分子聚合物HFMC后对睾丸组织结构的影响

本实验选用具有生育力成年雄性猕猴７只，在直视下行双侧ＨＦＭＣ输精管内注射，每侧剂量分别为３０ｍｇ１只，６０ｍｇ和１００ｍｇ各３只；于注射后２．５年和３．５年分别处死动物，取睾丸组织进行光镜和电镜观察．结果发现：猕猴注射ＨＦＭＣ２．５年后，睾丸光镜大部分曲细精管生精上皮结构完整，排列整齐。仅见局部少数管腔生精上皮层数减少，上皮细胞轻度水样变性等病理改变。电镜下曲细精管内除支持细胞内脂褐素增多，轻度基底膜增厚和精母细胞内质网扩张外，各级生精细胞，支持细胞及细胞间连接复合体等超微结构未见明显异常。注射ＨＦＭＣ３．５年后猕猴的光镜、电镜结果与注射后２．５年结果相似，但局部改变较２．５年组轻。上述结果表明：猕猴输精管内注射一定剂量ＨＦＭＣ节育不会引起睾丸组织的严重病理改变。但是，由于注射ＨＦＭＣ后，ＨＦＭＣ释放Ｈ＋及其对输精管的暂时阻塞，改变了精子生存的内环境，使睾丸出现局部轻度病理改变，随着ＨＦＭＣ逐渐溶解排出，睾丸功能相继恢复正常，配对产仔。为ＨＦＭＣ应用提供了安全性依据。     

老年学习记忆减退大鼠齿状回突触的定量研究

据老年大鼠在Ｍｏｒｒｉｓ水迷宫中的行为表现，将其分为老年学习记忆减退和学习记忆正常两部分，采用透射电镜观察、拍片，对齿状回中分子层触的数量和大小进行体视学定量分析。     

Osteogenic Cells Derived From Embryonic Stem Cells Produced Bone Nodules in Three-Dimensional Scaffolds

An approach for 3D bone tissue generation fromembryonic stem (ES) cells was investigated. The ES cells wereinduced to differentiate into osteogenic precursors, capable ofproliferating and subsequently differentiating into bone-formingcells. The differentiated cells and the seeded scaffolds werecharacterized using von Kossa and Alizarin Red staining, electronmicroscopy, and RT-PCR analysis. The results demonstrated thatES-derived bone-forming cells attached to and colonized thebiocompatible and biodegradable scaffolds. Furthermore, thesecells produced bone nodules when grown for 3–4 weeks inmineralization medium containing ascorbic acid andbeta-glycerophosphate both in tissue culture plates and inscaffolds. The differentiated cells also expressed osteospecificmarkers when grown both in the culture plates and in 3Dscaffolds. Osteogenic cells expressed alkaline phosphatase,osteocalcin, and osteopontin, but not an ES cell-specific marker,oct-4. These findings suggest that ES cell can be usedfor in vitro tissue engineering and cultivation of graftable skeletal structures.     

Modification of collagen matrices for enhancing angiogenesis

The vascularization of engineered tissues in many cases does not keep up with the ingrowth of cells. Nutrient and oxygen supply are not sufficient, which ultimately leads to the death of the invading cells. The enhancement of the angiogenic capabilities of engineered tissues therefore represents a major challenge in the field of tissue engineering. The immobilization of angiogenic growth factors may be useful for enhancing angiogenesis. The most potent angiogenic growth factor specific to endothelial cells, vascular endothelial growth factor (VEGF), occurs in several splice variants. The variant with 165 amino acids both has a high angiogenic activity and a high affinity for heparin. We therefore incorporated heparin molecules into collagen matrices by covalently cross-linking them to amino functions on the collagen. Physical binding of VEGF to the heparin may then prevent a rapid clearance from the implant, while the release rate may become coupled to the degradation of the collagen matrix. The modified matrices were characterized by determination of the extent of the heparin immobilization, the in vitro degradation rate by collagenase. For testing the angiogenic properties, non-modified and heparinized collagen specimens were--either loaded with VEGF or non-loaded--subcutaneously implanted on the back of rats. Specimens were explanted after varying periods of implantation, the dry weights and the hemoglobin contents, as well as immunostained histological sections were evaluated: heparinized collagen matrices loaded with VEGF are vascularized to a substantially higher extent as compared to non-modified matrices.     

^60Coγ射线全身照射后大鼠空肠亮脑啡肽的变化

应用免疫细胞化学(ICC)和放射免疫分析(RIA)方法观察了大鼠4、25Gy~(60)Coγ射线照射后24,48和,72h空肠亮脑啡肽(L-ENK)样免疫反应性神经的分布和L-ENK含量的变化。结果:4Gy照射后24h以及25Gy照射后24,48和72h光镜下见空肠L-ENK样免疫反应性神经的分布密度稍有增加,4Gy照射后24h以及25Gy照射后24,48和72h空肠L-ENK含量分别为29.81±0.84pg/mg,34.96±4.38pg/mg,40.71±3.62pg/mg和38.93±2.31pg/mg,与正常对照组(18.26±1.95pg/mg)相比明显升高(P<9.01)。