全文获取类型
收费全文 | 5002篇 |
免费 | 264篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 18篇 |
儿科学 | 165篇 |
妇产科学 | 166篇 |
基础医学 | 815篇 |
口腔科学 | 77篇 |
临床医学 | 420篇 |
内科学 | 863篇 |
皮肤病学 | 96篇 |
神经病学 | 545篇 |
特种医学 | 155篇 |
外科学 | 393篇 |
综合类 | 60篇 |
预防医学 | 481篇 |
眼科学 | 285篇 |
药学 | 402篇 |
中国医学 | 8篇 |
肿瘤学 | 327篇 |
出版年
2023年 | 20篇 |
2022年 | 58篇 |
2021年 | 81篇 |
2020年 | 46篇 |
2019年 | 70篇 |
2018年 | 96篇 |
2017年 | 56篇 |
2016年 | 108篇 |
2015年 | 120篇 |
2014年 | 147篇 |
2013年 | 196篇 |
2012年 | 323篇 |
2011年 | 390篇 |
2010年 | 193篇 |
2009年 | 185篇 |
2008年 | 335篇 |
2007年 | 314篇 |
2006年 | 332篇 |
2005年 | 330篇 |
2004年 | 292篇 |
2003年 | 257篇 |
2002年 | 283篇 |
2001年 | 48篇 |
2000年 | 30篇 |
1999年 | 66篇 |
1998年 | 90篇 |
1997年 | 77篇 |
1996年 | 58篇 |
1995年 | 48篇 |
1994年 | 45篇 |
1993年 | 42篇 |
1992年 | 35篇 |
1991年 | 24篇 |
1990年 | 18篇 |
1989年 | 14篇 |
1988年 | 35篇 |
1987年 | 19篇 |
1986年 | 22篇 |
1985年 | 24篇 |
1984年 | 19篇 |
1983年 | 19篇 |
1982年 | 17篇 |
1981年 | 15篇 |
1980年 | 18篇 |
1979年 | 14篇 |
1978年 | 15篇 |
1977年 | 14篇 |
1974年 | 14篇 |
1964年 | 13篇 |
1962年 | 13篇 |
排序方式: 共有5276条查询结果,搜索用时 11 毫秒
101.
Yang Li Zhenzhen Shi Isabella Radauer-Preiml Ancuela Andosch Eudald Casals Ursula Luetz-Meindl 《Nanotoxicology》2017,11(9-10):1157-1175
Nanoparticles (NPs) are easily contaminated by bacterial endotoxin (lipopolysaccharide [LPS]). The presence of LPS can be responsible for many immune/inflammatory effects attributed to NPs. In this study, we examined the effects of LPS adsorption on the NP surface on the formation of a biocorona in biological fluids and on the subsequent inflammation-inducing activity of NPs. Different gold (Au) NPs with sizes ranging from 10 to 80?nm and with different surface functionalization (sodium citrate, lipoic acid, and branched polyethyleneimine (BPEI), or polyethylene glycol (PEG)) were exposed to E. coli LPS under different conditions. The binding capacity of LPS to the surface of AuNPs was dose- and time-dependent. LPS attached to sodium citrate and lipoic acid coatings, but did not adhere to BPEI- or PEG-coated NPs. By computational simulation, the binding of LPS to AuNPs seems to follow the Langmuir absorption isotherm. The presence of LPS on AuNP surface interfered and caused a decrease in the formation of the expected biomolecular corona upon incubation in human plasma. LPS-coated AuNPs, but not the LPS-free NPs, induced significant inflammatory responses in vitro. Notably, while free LPS did also induce an anti-inflammatory response, LPS bound to NPs appeared unable to do so. In conclusion, the unintentional adsorption of LPS onto the NP surface can affect the biocorona formation and the inflammatory properties of NPs. Thus, for an accurate interpretation of NP interactions with cells, it is extremely important to be able to distinguish the intrinsic NP biological effects from those caused by biologically active contaminants such as endotoxin. 相似文献
102.
Domínguez-González Cristina Fernández-Torrón Roberto Moore Ursula de Fuenmayor-Fernández de la Hoz Carlos Pablo Vélez-Gómez Beatriz Cabezas Juan Antonio Alonso-Pérez Jorge González-Mera Laura Olivé Montse García-García Jorge Moris Germán León Hernández Juan Carlos Muelas Nuria Servian-Morilla Emilia Martin Miguel A. Díaz-Manera Jordi Paradas Carmen 《Journal of neurology》2022,269(7):3550-3562
Journal of Neurology - TK2 deficiency (TK2d) is a rare mitochondrial disorder that manifests predominantly as a progressive myopathy with a broad spectrum of severity and age of onset. The rate of... 相似文献
103.
Infectious Complications in Children With Acute Myeloid Leukemia and Down Syndrome: Analysis of the Prospective Multicenter Trial AML‐BFM 2004 下载免费PDF全文
104.
Anna-Lena Forst Vlad Sorin Olteanu Géraldine Mollet Tanja Wlodkowski Franz Schaefer Alexander Dietrich Jochen Reiser Thomas Gudermann Michael Mederos y Schnitzler Ursula Storch 《Journal of the American Society of Nephrology : JASN》2016,27(3):848-862
Podocytes are specialized, highly differentiated epithelial cells in the kidney glomerulus that are exposed to glomerular capillary pressure and possible increases in mechanical load. The proteins sensing mechanical forces in podocytes are unconfirmed, but the classic transient receptor potential channel 6 (TRPC6) interacting with the MEC-2 homolog podocin may form a mechanosensitive ion channel complex in podocytes. Here, we observed that podocytes respond to mechanical stimulation with increased intracellular calcium concentrations and increased inward cation currents. However, TRPC6-deficient podocytes responded in a manner similar to that of control podocytes, and mechanically induced currents were unaffected by genetic inactivation of TRPC1/3/6 or administration of the broad-range TRPC blocker SKF-96365. Instead, mechanically induced currents were significantly decreased by the specific P2X purinoceptor 4 (P2X4) blocker 5-BDBD. Moreover, mechanical P2X4 channel activation depended on cholesterol and podocin and was inhibited by stabilization of the actin cytoskeleton. Because P2X4 channels are not intrinsically mechanosensitive, we investigated whether podocytes release ATP upon mechanical stimulation using a fluorometric approach. Indeed, mechanically induced ATP release from podocytes was observed. Furthermore, 5-BDBD attenuated mechanically induced reorganization of the actin cytoskeleton. Altogether, our findings reveal a TRPC channel-independent role of P2X4 channels as mechanotransducers in podocytes. 相似文献
105.
Roselena Silvestri Schuh Juliana Bidone Edina Poletto Camila Vieira Pinheiro Gabriela Pasqualim Talita Giacomet de Carvalho Mirian Farinon Dirnete da Silva Diel Ricardo Machado Xavier Guilherme Baldo Ursula Matte Helder Ferreira Teixeira 《Pharmaceutical research》2018,35(11):221
Purpose
This study demonstrates the nasal administration (NA) of nanoemulsions complexed with the plasmid encoding for IDUA protein (pIDUA) as an attempt to reach the brain aiming at MPS I gene therapy.Methods
Formulations composed of DOPE, DOTAP, MCT (NE), and DSPE-PEG (NE-PEG) were prepared by high-pressure homogenization, and assessed in vitro on human fibroblasts from MPS I patients and in vivo on MPS I mice for IDUA production and gene expression.Results
The physicochemical results showed that the presence of DSPE-PEG in the formulations led to smaller and more stable droplets even when submitted to dilution in simulated nasal medium (SNM). In vitro assays showed that pIDUA/NE-PEG complexes were internalized by cells, and led to a 5% significant increase in IDUA activity, besides promoting a two-fold increase in IDUA expression. The NA of pIDUA/NE-PEG complexes to MPS I mice demonstrated the ability to reach the brain, promoting increased IDUA activity and expression in this tissue, as well as in kidney and spleen tissues after treatment. An increase in serum IL-6 was observed after treatment, although with no signs of tissue inflammatory infiltrate according to histopathology and CD68 assessments.Conclusions
These findings demonstrated that pIDUA/NE-PEG complexes could efficiently increase IDUA activity in vitro and in vivo after NA, and represent a potential treatment for the neurological impairment present in MPS I patients.106.
Prognostic value of natriuretic peptides in severe sepsis 总被引:1,自引:0,他引:1
107.
108.
Denk Gabriele Frede Ursula Gratzer Irene Kittinger-Sereinig Katharina Kolmorgen Katja Kriz Jakob 《Zeitschrift für Psychodrama und Soziometrie》2022,21(2):305-321
Zeitschrift für Psychodrama und Soziometrie - In diesem Artikel der Zeitschrift für Psychodrama und Soziometrie geben fünf Psychodramatiker*innen Einblick in ihren Praxisalltag und... 相似文献
109.
110.