全文获取类型
收费全文 | 1588篇 |
免费 | 80篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 33篇 |
妇产科学 | 18篇 |
基础医学 | 235篇 |
口腔科学 | 52篇 |
临床医学 | 93篇 |
内科学 | 312篇 |
皮肤病学 | 36篇 |
神经病学 | 110篇 |
特种医学 | 127篇 |
外科学 | 312篇 |
综合类 | 6篇 |
预防医学 | 24篇 |
眼科学 | 35篇 |
药学 | 89篇 |
中国医学 | 4篇 |
肿瘤学 | 175篇 |
出版年
2023年 | 9篇 |
2022年 | 19篇 |
2021年 | 52篇 |
2020年 | 21篇 |
2019年 | 28篇 |
2018年 | 34篇 |
2017年 | 38篇 |
2016年 | 48篇 |
2015年 | 31篇 |
2014年 | 42篇 |
2013年 | 72篇 |
2012年 | 104篇 |
2011年 | 112篇 |
2010年 | 62篇 |
2009年 | 54篇 |
2008年 | 103篇 |
2007年 | 110篇 |
2006年 | 115篇 |
2005年 | 110篇 |
2004年 | 113篇 |
2003年 | 98篇 |
2002年 | 107篇 |
2001年 | 13篇 |
2000年 | 14篇 |
1999年 | 23篇 |
1998年 | 20篇 |
1997年 | 12篇 |
1996年 | 17篇 |
1995年 | 13篇 |
1994年 | 10篇 |
1993年 | 8篇 |
1992年 | 10篇 |
1991年 | 14篇 |
1990年 | 7篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1975年 | 1篇 |
1965年 | 1篇 |
1960年 | 1篇 |
排序方式: 共有1674条查询结果,搜索用时 453 毫秒
31.
Toshinori Suzuki Hiroyuki Morishita Kosumo Fukuhara 《Journal of Clinical Biochemistry and Nutrition》2021,68(3):215
Kynurenic acid, a tryptophan metabolite, acts as antagonist or agonist of several receptors. Hypobromous acid (HOBr) and hypochlorous acid (HOCl) are generated by eosinophils and neutrophils. At inflammation sites, kynurenic acid may encounter HOBr and HOCl to generate products. When kynurenic acid was incubated with HOBr under neutral conditions, kynurenic acid generated a single product almost exclusively. This was identified as 3-bromokynurenic acid. Kynurenic acid reacted with HOCl, generating two products. The major product was identified as 3-chlorokynurenic acid with its oxidative decarboxylation product, 3-chloro-4-hydroxy-2(1H)-quinolinone as a by-product. Free amino acids suppressed the reactions of kynurenic acid with HOBr and HOCl. Taurine suppressed the HOCl reaction but not the HOBr reaction. An eosinophil peroxidase system containing H2O2, NaCl, and NaBr reacted with kynurenic acid, generating 3-bromokynurenic acid under mildly acidic conditions. Although a myeloperoxidase system containing H2O2 and NaCl reacted with kynurenic acid to generate 3-chlorokynurenic acid under mildly acidic conditions, the product was altered to 3-bromokynurenic acid by addition of NaBr to the system. These results suggest that 3-bromokynurenic acid and 3-chlorokynurenic acid may be generated from kynurenic acid at inflammation sites in humans, although their formation will be suppressed by coexistent amino acids. 相似文献
32.
33.
Minamiyama Shuhei Iwai Toshinori Sugiyama Satomi Hayashi Yuichiro Hirota Makoto Mitsudo Kenji 《Oral Radiology》2021,37(1):125-129
Oral Radiology - We report a rare case of schwannoma arising from the sublingual glandular branch of the lingual nerve radiologically masquerading as sublingual gland tumor. A 42-year-old female... 相似文献
34.
Satoshi Kutsuna Yusuke Asai Kei Yamamoto Michinori Shirano Keiji Konishi Tomohiro Asaoka Masaya Yamato Yukiko Katsuragi Yudai Yamamoto Toshinori Sahara Aya Tamiya Fukumi Nakamura-Uchiyama Naoya Sakamoto Atsushi Kosaka Takuya Washino Ryota Hase Haruki Mito Takashi Kurita Norio Ohmagari 《Journal of infection and chemotherapy》2021,27(4):632-638
IntroductionThe epidemiology of infectious diseases in Japan remains undefined despite the increasing tourism. GeoSentinel, an epidemiological surveillance system for reporting imported infectious diseases, has only two participating facilities in Japan. Although the number of infectious diseases is reported by the National Institute of Infectious Diseases, there is no detailed clinical information about these cases. Therefore, we established J-RIDA (Japan Registry for Infectious Diseases from Abroad) to clarify the status of imported infectious diseases in Japan and provide detailed information.MethodsJ-RIDA was started as a registry of imported infectious diseases. Case registration began in October 2017. Between October 2017 and September 2019, 15 medical institutions participated in this clinical study. The registry collected information about the patient's age, sex, nationality, chief complaint, consultation date, date of onset, whether visit was made to a travel clinic before travel, blood test results (if samples were collected), travel history, and final diagnosis.ResultsOf the 3046 cases included in this study, 46.7% to Southeast Asia, 13.0% to Africa, 13.7% to East Asia, 11.5% to South Asia, 7.5% to Europe, 3.8% to Central and South America, 4.6% to North America, 3.9% to Oceania, and 2.8% to Central and west Asia. More than 85% of chief complaints were fever and general symptoms, gastrointestinal symptoms, respiratory symptoms, or dermatologic problems. The most common diseases were travelers’ diarrhea, animal bite, upper respiratory infection, influenza, and dengue fever.ConclusionsWe summarized two-year cases registered in Japan's imported infectious disease registry. These results will significantly contribute to the epidemiology in Japan. 相似文献
35.
Nobuyuki Ashizawa Takahiro Takazono Kaname Ohyama Yoji Nagasaki Masaki Okamoto Tatsuro Hirayama Kensuke Takahashi Hirotomo Yamanashi Masato Tashiro Naoki Hosogaya Takeshi Tanaka Kazuko Yamamoto Yuichi Fukuda Yoshifumi Imamura Toshinori Kawanami Taiga Miyazaki Toyomitsu Sawai Kiyoyasu Fukushima Hiroshi Mukae 《Journal of infection and chemotherapy》2021,27(7):1033-1038
IntroductionNumerous severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serological tests exists commercially; however, their performance using clinical samples is limited. Although insufficient to detect SARS-CoV-2 in the early phase of infection, antibody assays can be of great use for surveillance studies or for some coronavirus disease 2019 (COVID-19) patients presenting late to the hospital.MethodsThis study evaluated the sensitivity and specificity of four commercial SARS-CoV-2 lateral flow antibody tests using 213 serum specimens from 90 PCR-positive confirmed COVID-19 patients. Of 59 negative control sera, 50 were obtained from patients with other respiratory infectious diseases before COVID-19 pandemic began while nine were from patients infected with other respiratory viruses, including two seasonal coronaviruses.ResultsThe varied sensitivities for the four commercial kits were 70.9%, 65.3%, 45.1%, and 65.7% for BioMedomics, Autobio Diagnostics, Genbody, and KURABO, respectively, between sick days 1 and 155 in COVID-19 patients. The sensitivities of the four tests gradually increased over time after infection before sick day 5 (15.0%, 12.5%, 15.0%, and 20.0%); from sick day 11–15 (95.7%, 87.2%, 53.2%, and 89.4%); and after sick day 20 (100%, 100%, 68.6%, and 96.1%), respectively. For severe illness, the sensitivities were quite high in the late phase after sick day 15. The specificities were over 96% for all four tests. No cross-reaction due to other pathogens, including seasonal coronaviruses, was observed.ConclusionsOur results demonstrated the large differences in the antibody test performances. This ought to be considered when performing surveillance analysis. 相似文献
36.
Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria 总被引:20,自引:0,他引:20
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Masashi Narita Shigeomi Shimizu Toshinori Ito Thomas Chittenden Robert J. Lutz Hikaru Matsuda Yoshihide Tsujimoto 《Proceedings of the National Academy of Sciences of the United States of America》1998,95(25):14681-14686
Cytochrome c release and the mitochondrial permeability transition (PT), including loss of the transmembrane potential (Δψ), play an important role in apoptosis. Using isolated mitochondria, we found that recombinant Bax and Bak, proapoptotic members of the Bcl-2 family, induced mitochondrial Δψ loss, swelling, and cytochrome c release. All of these changes were dependent on Ca2+ and were prevented by cyclosporin A (CsA) and bongkrekic acid, both of which close the PT pores (megachannels), indicating that Bax- and Bak-induced mitochondrial changes were mediated through the opening of these pores. Bax-induced mitochondrial changes were inhibited by recombinant Bcl-xL and transgene-derived Bcl-2, antiapoptotic members of the Bcl-2 family, as well as by oligomycin, suggesting a possible regulatory effect of F0F1-ATPase on Bax-induced mitochondrial changes. Proapoptotic Bax- and Bak-BH3 (Bcl-2 homology) peptides, but not a mutant BH3 peptide nor a mutant Bak lacking BH3, induced the mitochondrial changes, indicating an essential role of the BH3 region. A coimmunoprecipitation study revealed that Bax and Bak interacted with the voltage-dependent anion channel, which is a component of PT pores. Taken together, these findings suggest that proapoptotic Bcl-2 family proteins, including Bax and Bak, induce the mitochondrial PT and cytochrome c release by interacting with the PT pores. 相似文献
37.
38.
39.
40.
Chiaki Iwamura Kenta Shinoda Yusuke Endo Yukiko Watanabe Damon John Tumes Shinichiro Motohashi Kazuyoshi Kawahara Yuki Kinjo Toshinori Nakayama 《Proceedings of the National Academy of Sciences of the United States of America》2012,109(42):16992-16997
To develop more effective vaccines and strategies to regulate chronic inflammatory diseases, it is important to understand the mechanisms of immunological memory. Factors regulating memory CD4+ T helper (Th)-cell pool size and function remain unclear, however. We show that activation of type I invariant natural killer T (iNKT) cells with glycolipid ligands and activation of type II natural killer T (NKT) cells with the endogenous ligand sulfatide induced dramatic proliferation and expansion of memory, but not naïve, CD4 T cells. NKT cell-induced proliferation of memory Th1 and Th2 cells was dependent largely on the production of IL-2, with Th2-cell proliferation also affected by loss of IL-4. Type II NKT cells were also required for efficient maintenance of memory CD4 T cells in vivo. Activation of iNKT cells resulted in up-regulation of IFN-γ expression by memory Th2 cells. These IFN-γ–producing memory Th2 cells showed a decreased capability to induce Th2 cytokines and eosinophilic airway inflammation. Thus, activated NKT cells directly regulate memory CD4 T-cell pool size and function via the production of cytokines in vivo. 相似文献