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目的:基于数据挖掘技术探讨中药复方治疗过敏性紫癜肾炎的用药规律。方法:以中国方剂数据库为检索库,检索治疗"葡萄疫""紫癜""血尿""尿浊""水肿"的中药方剂,建立中药复方治疗过敏性紫癜肾炎的方剂数据库,并采用Excel、SPSS 20. 0软件进行频数及频率分析、因子分析等,进而对中药复方用药规律进行定量、定性分析并探讨其可能相关机制。结果:中药复方治疗过敏性紫癜肾炎涉及复方449首中药294种高频中药81味,茯苓、甘草、当归、生地黄、黄芩和防风较为常用;在功效方面补气药、理气药、利水消肿药、发散风寒药、补血药选用次数较多。四气以寒、温、平为主;五味以甘、苦、辛为主;以脾经、肺经、心经、肾经、肝经、胃经等6条经脉选用次数较多。最终提取了29个公因子,累积贡献率达100%,其中以补中益气、滋阴补血、利水渗湿为主;通过聚类分析,提取了6个常用得药对或药组。结论:过敏性紫癜肾炎的临床诊疗中,首先应辨明急性活动期和慢性缓解期,急性期以祛风散邪、活血化瘀之法治疗,用发散风寒、利水渗湿、凉血止血功效的中药;慢性期以补益之法,主要用补中益气、滋阴补血功效的中药,并选取相应中药组方治疗。 相似文献
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Synthesis of two substrate mimics of thioesterase in the biosynthesis of cyclic depsipeptide WS9326A
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Zhongyi Zhang Hanxuan Wang Guiyang Wang Xueyang Ma Tan Liu Tongtong Geng Xiaoxu Sun Donghui Yang Suwei Dong Ming Ma 《中国药学》2019,28(9):605-614
WS9326A is a tachykinin receptor antagonist and quorum sensing inhibitor discovered from several Streptomyces strains. The structure of WS9326A features a (Z)-pentenylcinnamoyl moiety attached on a cyclic depsipeptide skeleton, which is biosynthesized by nonribosomal peptide synthetases (NRPS). The regioselective cyclization in the last step of NRPS catalysis, which is proposed to be catalyzed by a thioesterase (TE) domain in the last module, has not been experimentally characterized. We here reportthe synthesis of two substrate mimics (1 and 2) of the TE (WS9326A-TE) in WS9326A biosynthesis, by using Fmoc-based solid-phase peptide synthesis (SPPS) method. Compounds 1 and 2 are new compounds whose structures have been elucidated based on NMR and HRESIMS analyses. The N-terminal cinnamoyl moiety and C-terminal methylated l-Ser moiety in 2 were incorporated under the mild SPPS conditions. Given the isolation difficulties of substrate of WS9326A-TE from the Streptomyces producers of WS9326A, our synthesis of 1 and 2 set the stage for the reconstitution of WS9326A-TE’s catalytic reaction in vitro in the future. 相似文献
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用于分子生物学中DNA快速提取技术的研究 总被引:2,自引:0,他引:2
本文建立了应用碘化钾快速提取DNA的方法,提取55份外周血及14份骨髓标本,用紫外分光光度计检测其纯度和浓度,并用于一系列分子试验加以验证获得满意效果。 相似文献
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Douglas B. Villaret Tongtong Wang Davin Dillon Jiangchun Xu Dhileep Sivam Martin A. Cheever Steven G. Reed 《The Laryngoscope》2000,110(3):374-381
Objectives/Hypothesis To discover unique genes specific for squamous cell carcinoma of the head and neck for eventual development as tumor markers and vaccine candidates. Study Design Molecular biological analysis of fresh‐frozen head and neck squamous cell cancer (HNSCC). Methods A subtractive library was made from two HNSCC and six normal tissues using a polymerase chain reaction (PCR)–based approach. Genes from this library were PCR amplified and placed on a microarray glass slide. RNA was prepared or obtained from 16 fresh‐frozen HNSCC and 22 normal tissue sources. Fluorescent probes were made from the polyA+ RNA derived from the tumor and normal tissues. The probes were hybridized to the glass slides and excited by a tuneable laser. One hundred seven of the genes showing the highest differential fluorescence value between tumor and normal tissue were identified by sequence analysis. Results Thirteen independent genes were found to be overexpressed in tumor tissues. Of these, nine were previously known: keratins K6 and K16, laminin‐5, plakophilin‐1, matrix metalloproteinase‐2 (MMP), vascular endothelial growth factor, connexin 26, 14–3‐3 sigma, and CaN19. The level of polyA+ RNA of these genes in the tumors was significantly different from the levels in normal tissue (P < .05). Four previously unidentified genes were also discovered to have increased expression in tumor tissue. Comparing the total tumor group (n = 16) to the normal group (n = 22), only one of these genes showed significant overexpression. Conclusion We report the identification of nine known genes that are significantly overexpressed in HNSCC as compared to normal tissue using subtractive and microarray technology. In addition, we present four previously unidentified genes that are overexpressed in a subset of tumors. These genes will be developed as tumor markers and vaccine candidates. 相似文献
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目的探讨多专科模拟实景演练医护培训提高急性心肌梗死患者救治时效性的价值。方法以中国胸痛中心认证中缩短急性ST段抬高型心肌梗死(STEMI)急诊经皮冠脉介入治疗(PCI)患者就诊至首次球囊扩张(DTB)时间的处理与救治措施为标准,由多科室护士长组成模拟实景演练培训小组,负责理论授课与设计实景演练方案,对105名相关科室护士和14名医生进行模拟实景演练培训。比较培训前280例(对照组)、培训后316例(观察组)STEMI急诊PCI患者的DTB各环节时间和预后情况。结果观察组DTB各环节时间显著缩短,DTB平均用时由104.64min缩短至77.45 min(均P0.01),患者平均住院日和费用显著缩短和减少(均P0.01)。结论运用模拟实景演练方法培训多科室护士明显缩短患者DTB时间,并显著提高急性心肌梗死患者救治时效性。 相似文献
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瘦素是维持人体能量代谢平衡的蛋白质,在人体中主要由白色脂肪组织分泌,通过与瘦素受体结合发挥作用.近年来有许多与瘦素相关的研究证明高血压患者及代谢综合征患者的血清瘦素水平较健康人群明显升高.两种疾病均可出现心室肥厚,蛋白尿,动脉粥样硬化等表现,说明二者存在共同的靶器官.瘦素代谢异常可出现瘦素抵抗并通过影响肾素-血管紧张素-醛固酮系统(renin angiotensin aldosterone system, RAAS)及炎症细胞因子来损伤靶器官.本文旨在总结瘦素在高血压及代谢综合征中的作用机制,并探讨瘦素对高血压合并代谢综合征靶器官损伤作用的研究进展. 相似文献