荧光定量PCR检测外周血中JAR细胞的探讨

目的 建立灵敏可靠的外周血中检出滋养细胞肿瘤细胞的方法,为早期诊断滋养细胞肿瘤的转移奠定实验基础。方法 在已知数量的ＪＡＲ细胞掺入１０ｍｌ外周血中建立肿瘤细胞血行转移实验模型,荧光定量逆转录－聚合酶链反应（ＦＱ－ＲＴ－ＰＣＲ）定量检测ＪＡＲ细胞的β－ｈＣＧ－ｍＲＮＡ。结果 ＦＱ－ＲＴ－ＰＣＲ可检测到相当于１个ＪＡＲ细胞表达的β－ｈＣＧ－ｍＲＮＡ量,但当ＪＡＲ细胞被掺入１０ｍｌ外周血中时,细胞数〉１     

Efficacy and safety of pemetrexed maintenance chemotherapy for advanced non-small cell lung cancer in a real-world setting

BackgroundPemetrexed maintenance therapy offers a survival benefit in patients with nonprogressive advanced nonsquamous non-small cell lung cancer (NSCLC) with good tolerability. This study was designed to analyze the efficacy and safety of pemetrexed maintenance chemotherapy in advanced nonsquamous NSCLC patients in a real-world setting.MethodsThe response rate (RR) and adverse events in 71 nonsquamous NSCLC patients treated with pemetrexed-based chemotherapy were observed until disease progression or unacceptable toxicities. Measures of survival were analyzed during follow-up.ResultsOf 69 efficacy-evaluable patients, the objective response rate (ORR) was 46.4% and the disease control rate (DCR) was 98.6%. ORR showed no significant difference between patients who received pemetrexed as first-line therapy and those who received pemetrexed as second-line or higher treatment. The median treatment cycle for all patients was 8. The median progression-free survival (PFS) was 9.5 months (m) and median overall survival (OS) was 30.5 m. The univariate and multivariate analyses showed that the number of chemotherapy cycles was an independent factor for PFS. The most common adverse reactions were grade 1 to 2 hematologic toxicities, gastrointestinal reactions, and liver enzyme abnormalities. Only 1 patient experienced a grade 3 gastrointestinal event.ConclusionsPemetrexed maintenance chemotherapy can improve PFS in patients with advanced nonsquamous NSCLC with good tolerability.     

The Autophagy Cargo Receptor SQSTM1 Inhibits Infectious Bursal Disease Virus Infection through Selective Autophagic Degradation of Double-Stranded Viral RNA

Selective autophagy mediates the degradation of cytoplasmic cargos, such as damaged organelles, invading pathogens, and protein aggregates. However, whether it targets double-stranded RNA (dsRNA) of intracellular pathogens is still largely unknown. Here, we show that selective autophagy regulates the degradation of the infectious bursal disease virus (IBDV) dsRNA genome. The amount of dsRNA decreased greatly in cells that overexpressed the autophagy-required protein VPS34 or autophagy cargo receptor SQSTM1, while it increased significantly in SQSTM1 or VPS34 knockout cells or by treating wild-type cells with the autophagy inhibitor chloroquine or wortmannin. Confocal microscopy and structured illumination microscopy showed SQSTM1 colocalized with dsRNA during IBDV infection. A pull-down assay further confirmed the direct binding of SQSTM1 to dsRNA through amino acid sites R139 and K141. Overexpression of SQSTM1 inhibited the replication of IBDV, while knockout of SQSTM1 promoted IBDV replication. Therefore, our findings reveal the role of SQSTM1 in clearing viral dsRNA through selective autophagy, highlighting the antiviral role of autophagy in the removal of the viral genome.     

补肾利湿法对急性痛风性关节炎大鼠血清IL-17表达水平的影响

目的：研究并观察补肾利湿法对痛风性关节炎大鼠白介素17（IL-17）表达水平的影响,探讨补肾利湿法防治急性痛风性关节炎（Gouty Arthritis,GA）的作用机制。方法：将84只Wistar大鼠随机分为7组：空白组（BG）、模型组（MG）、中药对照组(CMCG)、西药对照组(WMCG)及中药复方低、中、高浓度组(CMLG、CMMG、CMHG),每组12只。采用MSU联合草酰钾诱导大鼠急性痛风性关节炎模型,应用ELISA法,分别检测各组大鼠血清IL-17的含量。结果：与空白组比较,造模各组大鼠血清IL-17的水平明显升高,差异有显著统计学意义(P<0.01);与模型组比较,西药对照组、中药对照组、中药复方低、中、高浓度各组IL-17水平明显降低,差异有显著统计学意义（P<0.01）;西药对照组与中药复方中浓度组比较,IL-17含量差异不大（P>0.01）。结论：补肾利湿法处方可明显抑制急性痛风性关节炎大鼠IL-17的表达,对防治痛风性关节炎具有一定作用。     

女贞子调节糖脂代谢紊乱化学成分及作用机制研究进展

女贞子为木犀科植物女贞Ligustrum lucidum Ait.的干燥成熟果实.女贞子中的多糖、三萜、环烯醚萜以及黄酮类化合物能够通过增强胰岛素敏感性、改善胰岛素抵抗、保护胰岛β细胞;调控激活腺苷酸活化蛋白激酶(AMPK)、蛋白激酶B(AKT)、蛋白酪氨酸磷酸酶1B(PTP1B)信号通路;调节免疫、抑制炎症因子的释放...     

Study on the changes of Structural Covariance Network in post-traumatic stress disorder

Brain Imaging and Behavior - The topological properties of functional brain networks in post-traumatic stress disorder (PTSD) have been thoroughly examined, whereas the topology of structural...     

全身骨密度与肌肉、脂肪的相关性分析

目的 旨在进一步分析脂肪、肌肉与骨密度的相关性。方法 200例受检者,男性62例,女性138例,年龄20~84 (48. 9 ± 14. 2) y,体质量 42. 5 ~ 112. 0(61. 29 ± 9. 89) kg,BMI 15. 62~33.58( 23. 03 ± 2. 93) kg/ m2,应用 DXA (dual-energy X-ray absorptiometry,DXA)测量全身骨密度、肌肉、脂肪,并分析全身骨密度与肌肉、脂肪的相关性。结果 200例受检者,全身骨密度与体重、BMI的相关性有统计学意义(分别为r = 0. 530,P<0. 01;r = 0. 221,P<0.01),进一步分析发现,全身骨密度与肌肉呈正相关(r =0. 559,P < 0. 01),与脂肪的相关性无统计学意义(r =0. 018,P = 0. 803)。按性别分析,62例男性,全身骨密度与肌肉、脂肪均呈正相关(r =0. 535,P <0. 01;r =0.274,P = 0. 031)。138例女性,全身骨密度与肌肉呈正相关(r =0. 506,P < 0.01),而与脂肪的相关性无统计学意义(r =0. 088,P =0. 305)。结论（1)全身骨密度与体重、体质量指数呈正相关,其中,起主要作用的是肌肉组织,体现了“骨肉相连”的规律。（2)全身骨密度与脂肪的关系,在男性有相关性,而在女性无相关性, 体现了“男女有别”的规律。     

DXA测量人体质量与自测体重的差异分析

目的 体重在双能X线吸收法(dual-energy X-ray absorptiometry,DXA)诊断骨质疏松症方面有重要作用,由于季节不同穿着衣物重量的差异,可能对体重的结果有影响,本研究回顾性分析应用DXA测量人体质量与自测体重之间的差异。方法 观察200例样本,以体重与体质量的差值(difference of body weight and body mass,DBWM)为主要观察指标,体质量通过DXA 测量人体全身获得,体重通过体重秤直接测量,分析季节、性别因素对DBWM的影响。结果 测量的体重明显大于体质量（t =8. 432, P = 6. 846 x 10-15);体重与体质量在夏季基本一致（t = - 0. 376,P= 0. 712); DBWM在不同季节有不同分布（F= 3.662,P =0.013),夏季与其余季节比较均有明显差异(与春季、秋季、冬季比较分别为：P =0.003、P=0. 013、P = 0.002); DBWM的分布与性别无关(t =0. 554,P =0. 581);剔除15例奇异值样本之后,体重与体质量的差异仍存在（t = 14. 282,P = 4 951 x10-151)。结论 ①直接测量的体重明显大于DXA测量的体质量,不同季节对体重与体质量的差异有影响,由于夏季穿着衣物较少,对体重的影响也较小,建议在测量体重时穿着较少的衣物,或者扣除衣物的重量。②对于无法直接测量体重的样本,建议用DXA测量全身从而获得更准确的体质量。     

Improving the Autogenous Self-Sealing of Mortar: Influence of Curing Condition

With the construction of projects under severe environments, new and higher requirements are put forward for the properties of concrete, especially the autogenous self-sealing property, which is greatly affected by the curing environment and the state of the water. Herein, six types of curing conditions, including in air with a relative humidity of 30%, 60%, and 95%; flowing water; wet-dry cycles; and static water, are designed to investigate the autogenous self-sealing of mortar under different curing conditions. The results showed that the self-sealing ratios are higher than 60% and the cracks are closed for the mortar undergoing the wet-dry cycles and the static water. However, the self-sealing ratios of mortar are lower than 10% and the cracks are almost unchanged when the mortar is cured in the air with a relative humidity (RH) of 30% and 60%. The static liquid water is more conducive to the continued hydration of cement and the formation of CaCO₃ than the flowing water. The research provides guidance for the design of concrete and the improvement of autogenous self-sealing when the concrete serves in different environments.     

Some factors that affect the comparison between isotropic and orthotropic inhomogeneous finite element material models of femur

The objective of this study was to investigate whether there were significant differences between isotropic and orthotropic inhomogeneous material models of femur by taking into account the effects of some factors, such as comparative parameters, loading conditions and mesh refinement. Three femoral meshes of increasing refinement levels were assigned isotropic and orthotropic material properties. Then six different loading conditions were separately applied to each material model. Based on the analysis results of Von Mises stress and nodal displacement, eight regions of interest in femur were selected to compare the differences between isotropic and orthotropic material models. The results showed that marked differences for Von Mises stress (maximum 13.25%) and nodal displacement (maximum 15.04%) appeared in the regions where the maximum absolute Von Mises stress and the maximum absolute nodal displacement did not occur. It was observed that the comparison results were significantly different under different loading cases. The mesh refinement had a great influence on the comparison results, especially for the Von Mises stresses in the regions of the femoral neck. Therefore, it can be concluded that the differences between two material property assignments are significant, at least in some local regions.