SKP2在大肠癌组织中的表达及预后意义

目的:探讨SKP2在大肠癌中的表达和预后作用．方法:采用SP免疫组化法检测68例大肠癌手术切除组织标本中SKP2和P27的表达,用Kaplan-Meier和Cox回归分析法进行生存分析．结果:在68例大肠癌组织中,SKP2和P27的阳性表达率分别为41．2％(n=28)和52．9％(n= 36)．SKP2的表达与组织分级显著相关(X2= 14．073,P=0．001)．SKP2表达与年龄、性别和AJCC分期无关(P>0．05)．SKP2和P27负相关(r=-0．528,P=0．0001)．SKP2高表达组的总生存期较SKP2低表达组短(31．5±4．0 mo vs 54．5±2．1 mo,P<0．01)．多因素Cox回归分析表明,SKP2表达是大肠癌的独立预后因素(RR= 6．227．P=0．033)．结论:SKP2表达可以作为大肠癌患者预后的指标．     

GPR43 Suppresses Intestinal Tumor Growth by Modification of the Mammalian Target of Rapamycin Complex 1 Activity in ApcMin/+ Mice

ObjectiveG protein-coupled receptor 43 (GPR43), a receptor for short-chain fatty acids, plays a role in suppressing tumor growth; however, the detailed underlying mechanism needs to be comprehensively elucidated. In this study, we investigated the role of GPR43 in inhibiting tumor growth using Apc^Min/+, a murine model of intestinal tumors.Materials and MethodsUsing GPR43^−/− Apc^Min/+ and GPR43^+/− Apc^Min/+ mice, the number of tumors was analyzed at the end of the experimental period. Immunohistochemistry, quantitative polymerase chain reaction, and Western blotting were performed to analyze cellular proliferation and proliferation-associated signal pathways.ResultsOur results revealed that GPR43 deficiency resulted in increased tumor numbers in Apc^Min/+ mice. Ki67 was highly expressed in GPR43^−/− mice (p > 0.05). Increased expression levels of proinflammatory cytokines, including interleukin-6 and tumor necrosis factor-α, and amino acid transporters were not observed in GPR43-deficient mice compared to GPR43-sufficient mice. Furthermore, GPR43-deficient tumor tissues showed enhanced mammalian target of rapamycin-mediated phosphorylated ribosomal protein S6 kinase beta-1 (p > 0.05) and phosphorylated eukaryotic translation initiation factor 4E-binding protein 1 (p > 0.05), but not Akt (protein kinase B) phosphorylation (p = 0.7088).ConclusionCollectively, GPR43 affords protection against tumor growth at least partly through inhibition of the mammalian target of rapamycin complex 1 pathway.     

Synergistically assembled graphene/ZnO composite to enhance anticorrosion performance of waterborne epoxy coatings

In this work, waterborne epoxy resin and graphene/ZnO (Gr/ZnO) were employed as the matrix and nanofiller to construct composite coatings with enhanced anticorrosive performance. The corrosion protection properties of the coatings were significantly improved by the dispersed Gr sheets, as well as the parallelly assembled ZnO nanoparticles. The most remarkable improvement was achieved by adding 0.04 wt% of Gr and 0.4 wt% of ZnO in the Waterborne Epoxy (WEP) coatings, where the highest impedance was 200 530 Ω cm² on Gr_0.04–ZnO_0.4, far more than pure epoxy with 6186 Ω cm² after 7 days of immersion in electrolytes. Furthermore, the Gr_0.04–ZnO_0.4 coatings and corresponding corrosion products immersed in a 3.5% NaCl solution for 30 days were also characterized, which could further reveal anticorrosion mechanisms of the graphene modified WEP coatings and the passivated effect of ZnO. Through the mechanism analysis, we also found that ZnO could be employed as the barrier reinforcement to improve the dispersibility of graphene in WEP coatings, and the parallel assembly of graphene occurs spontaneously, leading to remarkable improvement of anticorrosion properties.

This is the first example of synergistically assembled Gr/ZnO sheets to improve the corrosion protection properties of WEP coatings, and Gr_0.04–ZnO_0.4 exhibited the highest impedance of 200 530 Ω cm² compared to pure WEP of 6186 Ω cm².     

2015年江苏省疟疾疫情流行病学分析

目的 掌握江苏省2015年疟疾疫情及流行特征,为如期实现本省的消除疟疾目标提供依据。方法 对2015年全省网络报告疟疾疫情和专报系统的流行病学调查资料进行统计分析。结果 2015年江苏省共报告疟疾405例,较2014年（355例）上升14.08%。均为境外输入病例,5例（1.23%）来自东南亚,400例（98.77%）来自25个非洲国家或地区。全省13个市均有输入性疟疾病例报告,其中泰州市、连云港、南通市、淮安市和扬州市报告病例数占全省总数的68.64%（278/405）。省疟疾诊断参比实验室对405份样本进行复核,发现恶性疟299例、间日疟13例、三日疟18例、卵形疟71例、混合感染4例。结论 江苏省已连续4年无本地感染疟疾病例,但境外输入性疟疾病例仍呈上升趋势,且输入性间日疟病例较2014年明显增加。加强境外输入性疟疾监测,提高各级医疗机构相关人员对疟疾的诊断和治疗能力、提升疾控机构专业人员病原复核、疫点调查和处置能力是本省疟疾防控工作的重点。     

内镜抗反流黏膜切除术治疗咽喉反流性疾病二例

本文对2例明确诊断咽喉反流性疾病患者行内镜抗反流黏膜切除术,术前、术后进行咽喉反流症状指数评分、反流体征评分、咽部pH监测、胃镜检查。对比术前、术后结果,上述评价指标均得到明显改善。     

胆汁酸参与结肠炎症和结肠癌发生的研究进展

结直肠癌的发生和发展与多种因素相关,其中胆汁酸因素逐渐引起学者们的重视.人体胆汁酸在相关因子的调解下,形成了具有生理功能并保持无毒作用的胆汁酸池.虽然胆汁酸相关代谢与肠道炎症存在复杂联系,但胆汁酸在结肠癌发生和发展中的确定机制尚未明确,本文主要对胆汁酸与结肠炎症和恶性肿瘤发生的相关性进行综述.     

心理护理在无痛人工流产术中的应用

目的观察心理护理综合措施用于无痛人工流产术的临床效果。方法将100例早孕妇女随机分为两组,每组50例。所有患者按常规过程进行术前准备和手术,试验组在进人手术室前根据患者所处状态和所提出的疑问有针对性地进行综合心理护理。结果进入手术室时试验组患者的焦虑程度明显小于对照组,且术后的主观感觉症状也明显优于对照组（P〈0．05）。同时,试验组患者较少发生不良反应。结论术前综合心理护理有利于无痛人工流产术者安全渡过围术期。     

A rapid and highly effective approach for SARS‐CoV‐2 nucleic acid daily testing in more than four thousand single‐tube samples

BackgroundPresently, the global spread of COVID‐19 is still going on, with more than 0.6 million new cases confirmed per day (as of November 20, 2021). However, since China entered a post‐epidemic phase in mid‐March 2020, the daily number of new domestic infections in the Chinese mainland has been maintained at almost zero or single digits, which was attributed to a series of effective measures for COVID‐19 prevention and control adopted by the Chinese government. Among these measures, SARS‐CoV‐2 nucleic acid testing holds key role for the timely confirmation and isolation of the infections to prevent further transmission.MethodsReferring to the national policy requirements, since April 30, 2020, The Affiliated Hospital of Qingdao University has conducted SARS‐CoV‐2 nucleic acid testing in its PCR laboratory for patients and social workers, as well as for environmental monitoring and employee screening. As of mid‐November 2020, the daily amount of single‐tube samples for nucleic acid testing rose above 4,000.ResultsIn this article, a rapid and highly effective approach for SARS‐CoV‐2 nucleic acid daily testing is presented, allowing five technicians to complete nucleic acid testing in 6,500 single‐tube samples in one day with a high level of quality. Using this approach, since the samples entered the PCR laboratory, all testing results were reported in 2.5–3 h with satisfactory quality control and precise reporting criterion as prerequisites.ConclusionThis testing approach provides a referable workflow for other testing institutions and is expected to play an important role in COVID‐19 prevention and control.     

The roles of the LpSTS and DLPFC in self‐prioritization: A transcranial magnetic stimulation study

The Self‐Attention Network (SAN) has been proposed to describe the underlying neural mechanism of the self‐prioritization effect, yet the roles of the key nodes in the SAN—the left posterior superior temporal sulcus (LpSTS) and the dorsolateral prefrontal cortex (DLPFC)—still need to be clarified. One hundred and nine participants were randomly assigned into the LpSTS group, the DLPFC group, or the sham group. We used the transcranial magnetic stimulation (TMS) technique to selectively disrupt the functions of the corresponding targeted region, and observed its impacts on self‐prioritization effect based on the difference between the performance of the self‐matching task before and after the targeted stimulation. We analyzed both model‐free performance measures and HDDM‐based performance measures for the self‐matching task. The results showed that the inhibition of LpSTS could lead to reduced performance in processing self‐related stimuli, which establishes a causal role for the LpSTS in self‐related processing and provide direct evidence to support the SAN framework. However, the results of the DLPFC group from HDDM analysis were distinct from the results based on response efficiency. Our investigation further the understanding of the differentiated roles of key nodes in the SAN in supporting the self‐salience in information processing.