Microactuator device for integrated measurement of epithelium mechanics

Mechanical forces are among important factors that drive cellular function and organization. We present a microfabricated device with on-chip actuation for mechanical testing of single cells. An integrated immersible electrostatic actuator system is demonstrated that applies calibrated forces to cells. We conduct stretching experiments by directly applying forces to epithelial cells adhered to device surfaces functionalized with collagen. We measure mechanical properties including stiffness, hysteresis and visco-elasticity of adherent cells.     

How are qualitative methods used in diabetes research? A 30-year systematic review

We aimed to describe how qualitative methods are used in global research on diabetes and identify opportunities whereby qualitative methods could further benefit our understanding of the human experience of diabetes and interventions to address it. We conducted a systematic review of National Library of Medicine, EMBASE, and Web of Science electronic databases to identify original research articles that used qualitative methods to study diabetes between 1980 and 2011. We identified 554 eligible articles and categorised these by geographic region, year of publication, study population, study design, research question, qualitative data collection methods, and journal type. Results show low use of qualitative methods in diabetes research over the past 30 years. The majority of articles (75%) reported using substantive qualitative research, while mixed-methods research has remained underutilised. Eighty-five per cent of articles reported studies conducted in North America or Europe, with few studies in developing countries. Most articles reported recruiting clinic-based populations (58%). Over half (54%) of research questions focused on patient experience and 24% on diabetes management. Qualitative methods can provide important insights about socio-cultural aspects of disease to improve disease management. However, they remain underutilised for understanding the diabetes experience, especially in Africa and Asia and amongst non-clinic populations.     

The Potential Role of Polymerase Chain Reaction in Diagnosis of Neonatal Herpes Simplex Virus Infection: Is Viral Culture Outdated?

Even in the era of effective antiviral therapy, neonatal herpes simplex viral infection causes significant morbidity and mortality in newborns. Prompt diagnosis is the cornerstone of treatment of these infants. Outside and inside the neonatal clinical practice, polymerase chain reaction (PCR) is replacing culture as a method of facilitating a speedy diagnosis of herpes simplex virus infection. New pediatric guidelines call for testing of high-risk asymptomatic infants, and thus, many more surface cultures and PCRs are being performed. This review aims to comprehensively describe the perinatal transmission of herpes simplex virus infection and the clinical presentation of neonatal herpes simplex disease, as well as to discuss whether PCR remains superior to culture methods.     

Polypectomy versus surgery in early colon cancer: size and location of colon cancer affect long-term survival

Background and Aims

The colon cancer survival rate is significantly affected by location, stage, and size of the cancer. Polypectomy was shown be as equally effective as surgery in early-stage colon cancer, but there have been no established clinical guidelines in the management of colon cancer based on the size of the polyp or the tumor location. The aim of our study was to assess the early-stage colon cancer-specific survival rate in patients who underwent endoscopic polypectomy versus surgery, based on size and location of tumor in early-stage colon cancer.

Methods

This is a population-based nationwide study in the USA.

Results

Of 13,157 patients, 15.5% underwent endoscopic treatment and 84.5% underwent surgical therapy. For early cancer tumors located in the left colon, polypectomy yielded comparable 5-year survivals to surgery irrespective of size of the tumors. Five-year early cancer-specific survivals were similar for tumors located in the right colon that were <?20 mm in size (94.5 vs 94.3%, p value?=?0.94). However, tumors >?20 mm in size that were located in the right colon had better survivals when treated surgically compared to those treated with polypectomy (20–39 mm: 91.8 vs 74.2%; ≥?40 mm: 92.4 vs 60%, both p values <?0.01). Similar results were obtained on propensity score analysis.

Conclusions

Polypectomy was as effective as surgical therapy for small tumors. For larger tumors, surgical therapy is better than polypectomy for right-sided tumors, but both are equally effective for left-sided tumors.

     

Imageability effects in normal Spanish–English bilingual adults and in aphasia: Evidence from naming to definition and semantic priming tasks

Background: Whereas the effect of imageability on lexical access has received attention in normal monolingual individuals and in individuals with aphasia, its effect on normal bilingual access and in bilingual aphasia has not been systematically addressed.Aim: The goal of the present experiment was to examine the effects of imageability in normal bilingual adults and in one patient with bilingual aphasia by addressing the following questions: (a) Is there a difference in language performance in early L2 bilinguals? (b) Is there a difference between concrete and abstract words across both languages? (c) Is there a difference between accuracy on a naming to definition task and semantic priming task across language and imageability?Methods & Procedures: A total of 15 normal Spanish–English bilingual adults and 1 bilingual aphasic individual performed two tasks – a naming to definition task and a semantic priming task in English and in Spanish. The targets in both tasks were either concrete or abstract nouns and the words were translation equivalents in the two languages. Naming accuracy in both languages and for both levels of imageability was measured during the naming to definition task. Mean reaction times and accuracy rates to judge relatedness of word pairs on the semantic priming task were also measured.Results: Results indicated that across tasks, performance was better in English than in Spanish, indicating an English dominance in the normal bilingual adults, although performance was the same across languages in the aphasic patient. Across tasks and languages, responses were faster and more accurate for concrete words than abstract words. Finally, retrieval of abstract words was significantly more difficult during naming to definition than during semantic priming, reflecting a processing difference between concrete and abstract words in retrieval of their respective phonological forms.Conclusions: These results highlight differences between concrete and abstract words in conceptual/semantic representations and phonological retrieval that are notably consistent across both languages in a bilingual individual. Data from the one bilingual aphasic individual suggest the possibility of a systematic deterioration of the normal bilingual language system.