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211.
Hasan Parvej Shahnaz Begum Ramkrishna Dalui Swarnali Paul Barun Mondal Subrata Sardar Nayim Sepay Gourhari Maiti Umesh Chandra Halder 《RSC advances》2022,12(27):17020
The binding of a small molecule to a protein through non-covalent interactions mainly depends on its size and electronic environment. Such binding can change the stability of the three dimensional protein structure which sometimes may destabilize it to accelerate or to inhibit protein aggregation. Coumarin is a widely used fluorescent dye with several biological applications. Different substituents (electron-donating and electron-withdrawing) at different positions of the coumarin moiety can influence its molecular volume, physical and chemical properties. Here we investigate the effect of such substituents of coumarin on the aggregation of a model protein, beta-lactoglobulin (β-lg) through a multi spectroscopic approach. It was observed that coumarin methyl ester with an 8-hydroxyl group can inhibit the β-lg aggregation. This compound can bind the hydrophobic site of beta-lactoglobulin and stabilize a particular protein conformation through the formation of hydrogen bond and hydrophobic interactions. Thus a properly designed compound can inhibit protein–protein interactions through protein–small molecule interactions. Other coumarinoid compounds also are effective in the prevention of thermal aggregation of β-lg.Aggregation of β-lactoglobulin (β-lg) was inhibited through the stabilization of the native structure by various non-covalent interactions of coumarin derivatives. The 8-hydroxy compound was most effective against the self-assembly of β-lg. 相似文献
212.
Microtiter ganglioside enzyme-linked immunosorbent assay for vibrio and Escherichia coli heat-labile enterotoxins and antitoxin. 总被引:15,自引:12,他引:15
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We have developed a microtiter enzyme-linked immunosorbent assay method for detecting the heat-labile enterotoxins of Vibrio cholerae and Escherichia coli using GM1 ganglioside as the base coat. This method compares favorably with a similar assay using anticholera toxin as the base coat, and with the Y1 adrenal cell assay. The assay should be useful in detecting enterotoxin production in E. coli and vibrios (including non-agglutinating Vibrio), in quantitating the toxin, and in determining binding properties of enterotoxins to ganglioside. The assay can also be used to quantitate antibodies which block the attachment of the toxin to the ganglioside. 相似文献
213.
214.
Development of Preliminary Remission Criteria for Gout Using Delphi and 1000Minds Consensus Exercises
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Hugh de Lautour William J. Taylor Ade Adebajo Rieke Alten Ruben Burgos‐Vargas Peter Chapman Marco A. Cimmino Geraldo da Rocha Castelar Pinheiro Ric Day Leslie R. Harrold Philip Helliwell Matthijs Janssen Gail Kerr Arthur Kavanaugh Dinesh Khanna Puja P. Khanna Chingtsai Lin Worawit Louthrenoo Geraldine McCarthy Janitzia Vazquez‐Mellado Ted R. Mikuls Tuhina Neogi Alexis Ogdie Fernando Perez‐Ruiz Naomi Schlesinger H. Ralph Schumacher Carlo A. Scirè Jasvinder A. Singh Francisca Sivera Ole Slot Lisa K. Stamp Anne‐Kathrin Tausche Robert Terkeltaub Till Uhlig Mart van de Laar Douglas White Hisashi Yamanaka Xuejun Zeng Nicola Dalbeth 《Arthritis care & research》2016,68(5):667-672
215.
Effect of Knee Extensor Strength on Incident Radiographic and Symptomatic Knee Osteoarthritis in Individuals With Meniscal Pathology: Data From the Multicenter Osteoarthritis Study
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218.
Sameer Naranje Sudeep Jain A. R. Nataraj Devdatt Neogi Baldeep Singh 《European journal of orthopaedic surgery & traumatology : orthopedie traumatologie》2009,19(2):113-115
Unicondylar fractures of the lower end of the femur are uncommon injuries. We report a case of fracture of the anterior aspect
of the lateral femoral condyle in coronal plane, which we termed as “Anterior Hoffa fracture”. With a different mechanism
of injury, this type of fracture needs to be differentiated from osteochondral fractures of the distal end of the femur for
proper management. After early debridement of the compound wound, we treated this case with open reduction and internal fixation.
During the total follow-up period of 8 months, the patient had near normal joint range of motion with no joint instability.
Open reduction and internal fixation with restoration of articular congruity is necessary for good functional outcome in this
rare type of injury. 相似文献
219.
220.
Zhang Y Terkeltaub R Nevitt M Xu L Neogi T Aliabadi P Niu J Felson DT 《Arthritis and rheumatism》2006,54(11):3508-3512
OBJECTIVE: Chondrocalcinosis, which can promote joint inflammation and cartilage degeneration, is highly prevalent in elderly white subjects. Data on its prevalence are scarce in other ethnic populations. This study was undertaken to compare the prevalence of chondrocalcinosis in Chinese subjects with that in white subjects. METHODS: We recruited a random sample of Beijing residents ages >or=60 years. Participants underwent standard weight-bearing anteroposterior knee radiography and posteroanterior hand radiography using the protocols developed in the Framingham Osteoarthritis Study. Radiographic chondrocalcinosis was defined as present in a knee or wrist when there was evidence of definite linear cartilage calcification. We compared the prevalence of chondrocalcinosis in Chinese subjects with that in white subjects using age-standardized prevalence ratios. We used identical methods to collect samples of tap water from 2 cities and measured their levels of calcium, magnesium, and phosphate in the same laboratory. RESULTS: Chinese subjects had a much lower prevalence of knee chondrocalcinosis (1.8% in men, 2.7% in women) than did white subjects (6.2% in men, 7.7% in women), with the age-standardized prevalence ratio being 0.34 (95% confidence interval [95% CI] 0.20-0.54) and 0.43 (95% CI 0.31-0.59) in men and women, respectively. Wrist chondrocalcinosis was rare in elderly Chinese subjects (prevalence 0.3% in men and 1.0% in women), with the age-standardized prevalence ratio being 0.06 (95% CI 0.01-0.18) in Chinese men and 0.18 (95% CI 0.10-0.30) in Chinese women. Calcium levels in the tap water in Beijing were 15-fold higher than in Framingham, whereas no difference was found in magnesium and phosphate levels. CONCLUSION: Knee chondrocalcinosis and wrist chondrocalcinosis are far less common in Chinese subjects in Beijing than in US white subjects in Framingham, Massachusetts. Given the current lack of understanding of the etiology of chondrocalcinosis, further epidemiologic studies of the impact of genetic and environmental factors on occurrence of chondrocalcinosis are indicated. 相似文献