吸烟者、慢性阻塞性肺疾病及肺心病患者PEFV曲线与MEFV曲线的对比探讨

目的　了解部分呼气流量 -容积 (PEFV)曲线的临床意义。方法　用 FJD- 80型及 L R- 80型肺功能仪测定了吸烟者、慢性阻塞性肺疾病 (COPD,慢阻肺 )和肺心病患者的 PEFV曲线和最大呼气流量 -容积(MEFV )曲线。结果　代表 PEFV曲线指标的 ·VF RC及 ·VFRC/ FVC,与 ·V75、·V50 、·V2 5的实 /预 (%)的变动 ,具有相同的倾向 ,都是被动吸烟组 >轻度吸烟组 >重度吸烟组 >慢支组 >肺气肿组 >肺心病组 ;且全部 ·VF RC、·VFRC/ FVC以及 ·V50 、·V2 5的实 /预 (%) ,在重度吸烟组都已降到 71%～ 76 %,而 ·V75在重度吸烟组和慢支组仍然大于 80 %。结论与 MEFV曲线相比 ,·VFRC、·VF RC/ FVC仍然是检查小气道功能的敏感指标 ,其下降的程度可反映疾病严重的程度。由于操作 PEFV曲线处于生理状态 ,易为患者接受 ,宜于临床推广应用。     

荧光定量聚合酶链反应检测乙肝病毒及其临床意义

OBJECTIVE: To study the relationship between the serum contents of HBV DNA copies and the HBV serum index of immunology and the degree of hepatocyte injury in patients with HBV hepatitis. METHODS: The contents of HBV DNA copies were detected by fluorescence quantitative PCR (FQ-PCR), and the HBV serum index of immunology by ELISA. RESULTS: The contents of HBV DNA copies had a significant difference between HBeAg (+)/anti-HBe (-) group (42 patients) and HBeAg (-)/anti-HBe (+) group (57 patients) or HBeAg (-)/anti-HBe (-) group(11 patients) (P < 0.05), while there was no significant difference between HBeAg (-)/anti-HBe (+) group and HBeAg (-)/anti-HBe (-) group(P > 0.05). The contents of HBV DNA copies had no correlation with the severe degree of hepatocyte injury(P > 0.05). CONCLUSION: Anti-HBe is not the terminal sign of HBV replication. The severe degree of hepatocyte injury has not a direct correlation with the contents of HBV DNA copies.     

经阴道超声诊断子宫内膜癌

目的 探讨应用经阴道超声（TVS）诊断绝经前后出血妇女子宫内膜癌的价值。方法 采用彩色多普勒超声诊断仪检测74例阴道异常出血的妇女，其中绝经前28例，绝经后46例，所有患者均取子宫内膜组织行病理对照。结果 TVS检测子宫内膜癌的敏感性、特异性、假阳性率、假阴性率、阳性预测值和阴性预测值，绝经前妇女依次为61．5％，50．0％，14．3％，23．8％，94．1％和54．5％，绝经后妇女依次为97．3％，55．6％，44．4％，2．7％，90．0％和83．0％。结论 TVS检测可作为子宫内膜癌首选的筛查方法，尤其对于绝经后妇女价值更大。     

复方广金钱草冲剂检验方法研究

应用薄层层析法，对制剂中广金钱草、黄芩、陈皮所含黄酮类成分，在同一色谱中得到的分离与鉴定，并以薄层一分光光度法对制剂中大黄所含成分大黄素进行了含量测定，方法重现性好，回收率为１００．１％，ＲＳＤ为１．０９％。     

Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: IV modulation of membrane fluidity of leukemic cell lines and tonsillar cell stimulated with McAbs

Summary In this study the technique of labelling the cell membrane with DPH fluorescence polarization was used to observe the membrane fluidity of B lymphocytic cell lines and tonsillar cells from healthy persons; the modulation effect on membrane-fluidity induced by McAbs against isotypic and idiotypic determinants of IgM from patients with leukemia was studied as well. The expression of the corresponding isotypic and idiotypic determinants of IgM on the cell membrane was determined. The results show that the membrane fluidity of leukemic cell lines is remarkably higher than that of tonsillar cells from healthy persons, and McAbs against isotypic determinants of leukemic IgM can enhance the membrane fluidity of all kinds of cells mentioned above. However, the anti-idiotypic monoclonal antibody increased only the membrane fluidity of leukemic cell lines. These results indicated that there was a close relationship between the effect of McAbs on cell membrane fluidity and the expression of corresponding isotypic and idiotypic determinants of IgM on the cell membrane.     

参芪胶囊的鉴别与含量测定方法研究

对参区胶囊中黄芪、人参、当归、知母、黄连等味药进行了薄层鉴别。并对黄连中小檗碱进行了薄层光密度法测定，方法灵敏，重现性好，回收率１００％，相对标准偏差ＲＳＤ％＝２．０（ｎ＝６）。     

Coexistence of autonomic and somatic mechanisms in the pressor areas of medulla in cats.

The effects of electrical stimulation and microinjection of sodium glutamate (0.5 M) in the sympathetic pressor areas of the dorsal medulla (DM), ventrolateral medulla (VLM), and parvocellular nucleus (PVC) on the knee jerk, crossed extension, and evoked potential of the L5 ventral root produced by intermittent electrical stimulation were studied in 98 adult cats anesthetized with chloralose and urethane. During electrical and glutamate stimulation of these pressor areas, in addition to the rise of systemic arterial blood pressure marked inhibition of the spinal reflex was produced, indicating presence of neuronal perikarya responsible for these actions. Mild to moderate augmentation of spinal reflexes was also observed during brain stimulation but only in a few cases. The magnitude of the somatic effects among the pressor areas of the VLM, DM, and PVC subsequent to glutamate activation was about the same. Induced spinal reflex inhibition, independent from the baroreceptor and vagal influence, remained essentially unaltered after acute midcollicular decerebration. The inhibition was also observed in cats decerebellated 8-10 days in advance. The inhibition was not affected after bilateral electrolytic- or kainic-acid-induced lesions in the paramedian reticular nucleus (PRN). On the contrary, PRN-induced spinal reflex inhibition was attenuated after bilateral lesions in the DM or VLM. Data suggest that there coexists neuronal subpopulations in the VLM, DM, and PVC that can affect both the sympathetic pressor systems and spinal reflexes.     

老年主动脉瓣闭锁不全的彩色多普勒超声心动图研究

报告接受彩色多普勒超声心动图检查的老年患者162例，其中75例有不同程度的主要动脉瓣关闭不全（Ar)。老年（Ar)的病因构成与中，老年前期者明显不同，主动脉，瓣钙化及原因不明者的比例占多数（67％）。主动脉瓣钙化者Ar程度多较重。主动脉瓣钙化及原因不明Ar患者的其他三个瓣膜返流检出率及和程度均明显高于无Ar的老年患者。表明多瓣膜退行性病变是此类Ar患者的原因之一。     

Long-term results of Grice subtalar arthrodesis in children

Talipes valgus, mostly resulted from poliomyelitis, of 44 feet in 43 children, aged 4 to 12 years, were treated by Grice extra-articular arthrodesis of the subtalar joint, and on 36 of them tendon transfer was done as well at the same time. The result in all of them, after a period of 15.3 years (ranging 5 to 23 yrs.), was found satisfactory in 32 feet (72.7%), and unsatisfactory in 12 (27.3%). Eight of the latter were found to be due to secondary varus deformity, a consequence of imbalance of muscle power following medial transfer of peroneal tendon (s). It is emphasized that in correcting foot deformity, slight valgus is receptible while over correction (varus) must be avoided. It is also found that after subtalar stabilization, traumatic osteoarthritis of midtarsal and ankle joints developed more frequently in patients who had better limb function than in those whose limbs had functioned much less.