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981.
Sok Ying Liaw Lai Fun Wong Sally Wai-Chi Chan Jasmine Tze Yin Ho Siti Zubaidah Mordiffi Sophia Bee Leng Ang Poh Sun Goh Emily Neo Kim Ang 《Journal of medical Internet research》2015,17(1)
BackgroundWeb-based learning is becoming an increasingly important instructional tool in nursing education. Multimedia advancements offer the potential for creating authentic nursing activities for developing nursing competency in clinical practice.ObjectiveThis study aims to describe the design, development, and evaluation of an interactive multimedia Web-based simulation for developing nurses’ competencies in acute nursing care.MethodsAuthentic nursing activities were developed in a Web-based simulation using a variety of instructional strategies including animation video, multimedia instructional material, virtual patients, and online quizzes. A randomized controlled study was conducted on 67 registered nurses who were recruited from the general ward units of an acute care tertiary hospital. Following a baseline evaluation of all participants’ clinical performance in a simulated clinical setting, the experimental group received 3 hours of Web-based simulation and completed a survey to evaluate their perceptions of the program. All participants were re-tested for their clinical performances using a validated tool.ResultsThe clinical performance posttest scores of the experimental group improved significantly (P<.001) from the pretest scores after the Web-based simulation. In addition, compared to the control group, the experimental group had significantly higher clinical performance posttest scores (P<.001) after controlling the pretest scores. The participants from the experimental group were satisfied with their learning experience and gave positive ratings for the quality of the Web-based simulation. Themes emerging from the comments about the most valuable aspects of the Web-based simulation include relevance to practice, instructional strategies, and fostering problem solving.ConclusionsEngaging in authentic nursing activities using interactive multimedia Web-based simulation can enhance nurses’ competencies in acute care. Web-based simulations provide a promising educational tool in institutions where large groups of nurses need to be trained in acute nursing care and accessibility to repetitive training is essential for achieving long-term retention of clinical competency. 相似文献
982.
Keon Yeop Kim So Youn Jeon Man Joong Jeon Kwon Ho Lee Sok Goo Lee Dongjin Kim Eunjeong Kang Sang Geun Bae Jinhee Kim 《Yebang Ŭihakhoe chi》2012,45(4):267-275
Objectives
This study was conducted to assess the potential health impacts and improve the quality of the free immunization program in Jinju City by maximizing the predicted positive health gains and minimizing the negative health risks.Methods
A steering committee was established in September 2010 to carry out the health impact assessment (HIA) and began the screening and scoping stages. In the appraisal stage, analysis of secondary data, a literature review, case studies, geographic information systems analysis, a questionnaire, and expert consultations were used. The results of the data collection and analyses were discussed during a workshop, after which recommendations were finalized in a written report.Results
Increased access to immunization, comprehensive services provided by physicians, the strengthened role of the public health center in increasing immunization rates and services, and the ripple effect to other neighboring communities were identified as potential positive impacts. On the other hand, the program might be inaccessible to rural regions with no private clinics where there are more at-risk children, vaccine management and quality control at the clinics may be poor, and vaccines may be misused. Recommendations to maximize health gains and minimize risks were separately developed for the public health center and private clinics.Conclusions
The HIA provided an opportunity for stakeholders to comprehensively overview the potential positive and negative impacts of the program before it was implemented. An HIA is a powerful tool that should be used when developing and implementing diverse health-related policies and programs in the community. 相似文献983.
Analysis of antibodies against components of the autonomic nervous system in diabetes mellitus 总被引:1,自引:0,他引:1
Stroud CR; Heller SR; Ward JD; Hardisty CA; Weetman AP 《QJM : monthly journal of the Association of Physicians》1997,90(9):577-585
Antibodies to autonomic nervous system structures have previously been
detected using a complement fixation immunofluorescence test in the sera of
patients with insulin-dependent diabetes mellitus (IDDM) and non-insulin
dependent diabetes mellitus (NIDDM). These antibodies might play a role in
the aetiology of autonomic neuropathy. Sera from 45 IDDM, 40 NIDDM and 52
control subjects were tested by immunofluorescence for antibodies to human
sympathetic ganglia, human adrenal medulla and rabbit vagus nerve. The use
of human sympathetic ganglia was compared with rabbit tissue for the
detection of sympathetic ganglia antibodies; the results for these
autonomic nervous system antibodies were also compared with results using
an ELISA. There was no relationship between the presence of antibodies
detected by ELISA and those detected by immunofluorescence, but of 14 IDDM
patients with thyroid antibodies, 12 had autonomic nervous system
antibodies detected by either immunofluorescence or ELISA (p < 0.005
compared to patients without thyroid antibodies). To further characterize
the autoantigen(s), immunoblotting was performed. An adrenal antigen
corresponding to 74 kDa was detected in sera from three patients, only one
of whom had antibodies detectable by ELISA and immunofluorescence. One IDDM
serum showed specific binding to a vagus nerve antigen corresponding to 33
kDa. No specific binding to sympathetic ganglia antigen was demonstrated.
Antibodies against autonomic nervous system antigens are an inconsistent
feature of diabetes, and appear more associated with coincidental
autoimmunity against other organs such as the thyroid.
相似文献
984.
M Psichogiou ; A Katsoulidou ; E Vaindirli ; B Francis ; SR Lee ; A Hatzakis 《Transfusion》1997,37(8):858-862
BACKGROUND: The study of the sensitivity of screening assays is greatly facilitated by testing the sequential changes in seroconverting individuals. The aim of this study was to investigate the early immunologic response after hepatitis C virus (HCV) infection and to evaluate whether HCV envelope (E2) recombinant antigen would provide a significant increase in sensitivity for detection of anti-HCV. STUDY DESIGN AND METHODS: Twenty hemodialysis patients who were seroconverting to anti-HCV were included in this study. They were followed up for a mean period (+/− SD) of 10.5 +/− 3.3 months, in which 13 to 46 serum samples per case were collected. Each sample was tested for anti-HCV by second- and third-generation enzyme immunoassay (EIA-2 and EIA-3) and recombinant immunoblot assay (RIBA-3). E2 antibodies were tested by a prototype EIA in which E2 was expressed as a recombinant antigen in Chinese hamster ovary cells. RESULTS: Alanine aminotransferase elevation was observed in 18 of 20 cases. Reactivity against c100, c33c, c22, NS5, and E2 was detected in 15 (75%), 19 (95%), 15 (75%), 2 (10%), and 17 (85%) patients, respectively; c33c was the most immunogenic antigen, followed in descending order by E2, c22, c100, and NS5. E2 antibody reactivity resolved the two RIBA-3- indeterminate cases. However, there was no case in which E2 reactivity preceded all other HCV antigens. Anti-E2 was found to react in all patients of genotypes 1a, 1b, and 3a but in only 2 of 4 patients of genotype 4a. CONCLUSION: In this group of seroconverting individuals, E2 antigen was shown to be highly immunoreactive and did resolve some RIBA-3-indeterminate samples as being positive, on the basis of reactivity to multiple antigens, but it did not improve early detection of seroconversion. 相似文献
985.
Effect of enhanced homestead food production and aquaculture on dietary intakes of women and children in rural Cambodia: A cluster randomized controlled trial 下载免费PDF全文
Vashti Verbowski Zaman Talukder Kroeun Hou Ly Sok Hoing Kristina Michaux Victoria Anderson Rosalind Gibson Kathy H. Li Larry D. Lynd Judy McLean Tim J. Green Susan I. Barr 《Maternal & child nutrition》2018,14(3)
The Cambodian diet is low in nutrient‐dense animal‐source foods. Enhanced homestead food production (EHFP) and aquaculture, which increase availability of nutrient‐dense foods, are promising interventions to improve dietary intake. This study examined the effect of EHFP with or without aquaculture on dietary intake and prevalence of inadequate intake of select nutrients among women and children living in rural Cambodia, compared to controls. In a registered, cluster randomized controlled trial in Prey Veng, Cambodia, 10 households in each of 90 villages (n = 900) were randomized by village to receive EHFP, EHFP plus aquaculture, or control. After 22‐month intervention, 24‐hr dietary recalls (24HRs) were collected from mothers aged 18–50 years (n = 429) and their children aged 6 months–7 years (n = 421), reported by their mothers. Usual intake distributions (generated using 24HRs and repeat 24HRs on a subsample) were used to estimate prevalence of inadequate intake. Compared to controls, women in the EHFP group had significantly higher zinc (+1.0 mg/d) and Vitamin A (+139 retinol activity equivalents/d) intakes, and women in the EHFP plus aquaculture group had significantly higher iron (+2.7 mg/d), Vitamin A (+191 retinol activity equivalents/d), and riboflavin (+0.17 mg/d) intakes. Women in the EHFP plus aquaculture group also had significantly lower prevalence of inadequate iron (?7%, at 10% bioavailability), Vitamin A (?19%), and riboflavin (?17%) intakes, compared to controls. No significant differences in intakes or nutrient adequacy were observed among children or between EHFP and EHFP plus aquaculture groups. The biological importance of the small differences in nutrient intakes among women remains to be established. 相似文献
986.
高效液相色谱法同时测定盐酸维拉帕米及其主要代谢产物 总被引:6,自引:0,他引:6
建立了反相高效液相色谱法同时测定人血浆中维拉帕米及其主要代谢产物去甲维拉帕米血药浓度.以甲醇—水—三乙胺(67∶33∶0.4,pH6.7)为流动相,乙吗噻嗪(ethmosine)为内标,样品用正己烷—正丁醇混合液提取浓缩后进样,紫外检测器检测(279nm)。此法操作简便,精密度好,日内、日间误差:维拉帕米<8.6%,去甲维拉帕米<7.6%;方法回收率高,维拉帕米、去甲维拉帕米回收率均>92%。两者血药浓度在25~1000ng·ml-1范围内呈线性关系,最小检测浓度维拉帕米:2.5ng·ml-1,去甲维拉帕米:5.0ng·ml-1。应用该法测定了6名志愿者口服盐酸维拉帕米片剂后的血药浓度。 相似文献
987.
Senthilmani Rajendran Jian Han Lim Kohgulakuhan Yogalingam Thomas George Kallarakkal Rosnah Binti Zain Ruwan Duminda Jayasinghe Jyotsna Rimal Alexander Ross Kerr Rahmi Amtha Karthikeya Patil Roshan Alex Welikala Ying Zhi Lim Paolo Remagnino John Gibson Wanninayake Mudiyanselage Tilakaratne Chee Sun Liew Yi-Hsin Yang Sarah Ann Barman Chee Seng Chan Sok Ching Cheong 《Oral diseases》2023,29(5):2230-2238
988.
989.
Pagna Sok Aniko Sabo Lynn M. Almli Mary M. Jenkins Wendy N. Nembhard A. J. Agopian Michael J. Bamshad Elizabeth E. Blue Lawrence C. Brody Austin L. Brown Marilyn L. Browne Mark A. Canfield Suzan L. Carmichael Jessica X. Chong Shannon Dugan-Perez Marcia L. Feldkamp Richard H. Finnell Richard A. Gibbs Denise M. Kay Yunping Lei Qingchang Meng Cynthia A. Moore James C. Mullikin Donna Muzny Andrew F. Olshan Faith Pangilinan Jennita Reefhuis Paul A. Romitti Jeremy M. Schraw Gary M. Shaw Martha M. Werler Sanjiv Harpavat Philip J. Lupo University of Washington Center for Mendelian Genomics NISC Comparative Sequencing Program the National Birth Defects Prevention Study 《American journal of medical genetics. Part A》2023,191(6):1546-1556
The etiology of biliary atresia (BA) is unknown, but recent studies suggest a role for rare protein-altering variants (PAVs). Exome sequencing data from the National Birth Defects Prevention Study on 54 child–parent trios, one child–mother duo, and 1513 parents of children with other birth defects were analyzed. Most (91%) cases were isolated BA. We performed (1) a trio-based analysis to identify rare de novo, homozygous, and compound heterozygous PAVs and (2) a case–control analysis using a sequence kernel-based association test to identify genes enriched with rare PAVs. While we replicated previous findings on PKD1L1, our results do not suggest that recurrent de novo PAVs play important roles in BA susceptibility. In fact, our finding in NOTCH2, a disease gene associated with Alagille syndrome, highlights the difficulty in BA diagnosis. Notably, IFRD2 has been implicated in other gastrointestinal conditions and warrants additional study. Overall, our findings strengthen the hypothesis that the etiology of BA is complex. 相似文献
990.