Pattern of proteinuria in IgA nephropathy

Summary: Proteinuria is one of the bad prognostic indices in IgA nephritis (IgAN). This study compares the pattern of protein excretion in 10 patients with IgAN (IA) with that 5 years later (IB), when they developed renal impairment or hypertension. The pattern of proteinuria was analysed by SDS-PAGE and isoelectric focusing (IEF) and assayed for orosomucoid, α-1-microglobulin, retinol-binding protein, lysozyme, beta-2-microglobulin and N-acetyl-β-D-glucosaminidase activity. The data suggest that the changing pattern of proteinuria from IgA₁ to IgA₂ may reflect hyperfiltration as well as tubular injury.     

Enhanced cell mediated immunity in mice after cisplatin treatment

Tumor regression in mice after cisplatin treatment and the role of cell mediated immunity were studied through Winn assay (in vivo) and capillary cell migration inhibition assay (in vitro). The immunization of mice with the platinum complex results in specific enhancement of host cellular immune system by stimulating splenocytes and peritoneal exudate cells against the specific tumor antigens.