全文获取类型
收费全文 | 2642篇 |
免费 | 210篇 |
国内免费 | 7篇 |
专业分类
耳鼻咽喉 | 48篇 |
儿科学 | 52篇 |
妇产科学 | 30篇 |
基础医学 | 424篇 |
口腔科学 | 28篇 |
临床医学 | 235篇 |
内科学 | 476篇 |
皮肤病学 | 86篇 |
神经病学 | 348篇 |
特种医学 | 117篇 |
外科学 | 210篇 |
综合类 | 32篇 |
一般理论 | 2篇 |
预防医学 | 206篇 |
眼科学 | 52篇 |
药学 | 256篇 |
中国医学 | 8篇 |
肿瘤学 | 249篇 |
出版年
2024年 | 2篇 |
2023年 | 22篇 |
2022年 | 44篇 |
2021年 | 86篇 |
2020年 | 53篇 |
2019年 | 68篇 |
2018年 | 76篇 |
2017年 | 63篇 |
2016年 | 80篇 |
2015年 | 99篇 |
2014年 | 106篇 |
2013年 | 165篇 |
2012年 | 213篇 |
2011年 | 198篇 |
2010年 | 113篇 |
2009年 | 115篇 |
2008年 | 226篇 |
2007年 | 173篇 |
2006年 | 176篇 |
2005年 | 172篇 |
2004年 | 152篇 |
2003年 | 118篇 |
2002年 | 114篇 |
2001年 | 22篇 |
2000年 | 23篇 |
1999年 | 16篇 |
1998年 | 19篇 |
1997年 | 19篇 |
1996年 | 10篇 |
1995年 | 7篇 |
1994年 | 9篇 |
1993年 | 8篇 |
1992年 | 7篇 |
1991年 | 1篇 |
1990年 | 7篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 9篇 |
1986年 | 8篇 |
1985年 | 11篇 |
1984年 | 13篇 |
1983年 | 8篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 4篇 |
1977年 | 1篇 |
排序方式: 共有2859条查询结果,搜索用时 843 毫秒
61.
Isabella Gruber Ursel Heudorf Guido Werner Yvonne Pfeifer Can Imirzalioglu Hanns Ackermann Christian Brandt Silke Besier Thomas A. Wichelhaus 《International journal of medical microbiology : IJMM》2013,303(8):405-409
Colonization/infection with multidrug-resistant bacteria (MDRB) such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), and extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae, is an increasing problem not only in hospitals but also in long-term care facilities. The aim of this study was to determine the prevalence as well as the risk factors of colonization/infection with MRSA, VRE, and ESBL producing Enterobacteriaceae in geriatric clinics, nursing homes, and ambulant care in Frankfurt am Main, Germany. 288 patients from 2 geriatric clinics (n = 46), 8 nursing homes (n = 178), and 2 ambulant care facilities (n = 64) as well as 64 staff members were screened for MDRB in the time period from October 2006 to May 2007. 58 patients (20.1%) and 4 staff members (6.2%) were colonized with MDRB. Among patients, 27 (9.4%) were colonized with MRSA, 11 (3.8%) were screened positive for VRE, and 25 (8.7%) were found to be colonized with ESBL producing Enterobacteriaceae. Prevalence of MDRB in geriatric clinics, nursing homes, and ambulant care facilities were 32.6%, 18.5%, and 15.6%, respectively. Significant risk factors for MDRB were immobility (OR: 2.7, 95% CI: 1.5–4.9; p = 0.002), urinary catheter (OR: 3.1, 95% CI: 1.7–5.9; p < 0.001), former hospitalization (OR: 2.1, 95% CI: 1.1–4.0; p = 0.033), and wounds/decubiti (OR: 2.3, 95% CI: 1.5–4.9; p = 0.03). Finally, the high level of MDRB in geriatric clinics, nursing homes, and ambulant care points to the importance of these institutions as a reservoir for dissemination. 相似文献
62.
63.
Silke Keller Anke Liedek Dalia Shendi Monika Bach Günter E. M. Tovar Petra J. Kluger Alexander Southan 《RSC advances》2020,10(58):35273
Azide-bearing cell-derived extracellular matrices (“clickECMs”) have emerged as a highly exciting new class of biomaterials. They conserve substantial characteristics of the natural extracellular matrix (ECM) and offer simultaneously small abiotic functional groups that enable bioorthogonal bioconjugation reactions. Despite their attractiveness, investigation of their biomolecular composition is very challenging due to the insoluble and highly complex nature of cell-derived matrices (CDMs). Yet, thorough qualitative and quantitative analysis of the overall material composition, organisation, localisation, and distribution of typical ECM-specific biomolecules is essential for consistent advancement of CDMs and the understanding of the prospective functions of the developed biomaterial. In this study, we evaluated frequently used methods for the analysis of complex CDMs. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and (immune)histochemical staining methods in combination with several microscopic techniques were found to be highly eligible. Commercially available colorimetric protein assays turned out to deliver inaccurate information on CDMs. In contrast, we determined the nitrogen content of CDMs by elementary analysis and converted it into total protein content using conversion factors which were calculated from matching amino acid compositions. The amount of insoluble collagens was assessed based on the hydroxyproline content. The Sircol™ assay was identified as a suitable method to quantify soluble collagens while the Blyscan™ assay was found to be well-suited for the quantification of sulphated glycosaminoglycans (sGAGs). Eventually, we propose a series of suitable methods to reliably characterise the biomolecular composition of fibroblast-derived clickECM.Common characterisation methods for cell-derived extracellular matrices (ECMs) are compared using both unmodified and azide-bearing fibroblast-derived ECM. 相似文献
64.
Sebastian Reuther Silke Szymczak Annette Raabe Kerstin Borgmann Andreas Ziegler Cordula Petersen Ekkehard Dikomey Ulrike Hoeller 《Strahlentherapie und Onkologie》2015,191(1):59-66
Background and purpose
The aim of this study was to determine the impact of functional single nucleotide polymorphism (SNP) pathways involved in the ROS pathway, DNA repair, or TGFB1 signaling on acute or late normal toxicity as well as individual radiosensitivity.Materials and methods
Patients receiving breast-conserving surgery and radiotherapy were examined either for erythema (n?=?83), fibrosis (n?=?123), or individual radiosensitivity (n?=?123). The 17 SNPs analyzed are involved in the ROS pathway (GSTP1, SOD2, NQO1, NOS3, XDH), DNA repair (XRCC1, XRCC3, XRCC6, ERCC2, LIG4, ATM) or TGFB signaling (SKIL, EP300, APC, AXIN1, TGFB1). Associations with biological and clinical endpoints were studied for single SNPs but especially for combinations of SNPs assuming that a SNP is either beneficial or deleterious and needs to be weighted.Results
With one exception, no significant association was seen between a single SNP and the three endpoints studied. No significant associations were also observed when applying a multi-SNP model assuming that each SNP was deleterious. In contrast, significant associations were obtained when SNPs were suggested to be either beneficial or deleterious. These associations increased, when each SNP was weighted individually. Detailed analysis revealed that both erythema and individual radiosensitivity especially depend on SNPs affecting DNA repair and TGFB1 signaling, while SNPs in ROS pathway were of minor importance.Conclusion
Functional pathways of SNPs may be used to form a risk score allowing to predict acute and late radiation-induced toxicity but also to unravel the underlying biological mechanisms.65.
Antibacterial photodynamic treatment of periodontopathogenic bacteria with indocyanine green and near‐infrared laser light enhanced by TroloxTM
下载免费PDF全文
![点击此处可从《Lasers in surgery and medicine》网站下载免费的PDF全文](/ch/ext_images/free.gif)
66.
67.
Relationship Between Minor Myocardial Damage and Inflammatory Acute-Phase Reaction in Acute Coronary Syndromes 总被引:10,自引:0,他引:10
Hoffmeister HM Ehlers R Büttcher E Steinmetz A Kazmaier S Helber U Szabo S Beyer ME Seipel L 《Journal of thrombosis and thrombolysis》2003,15(1):33-39
Background: In severe acute coronary syndromes (ACS) elevation of markers of inflammation and acute phase reaction (APR) like C-reactive protein (CRP) as well as a release of troponin have been reported.Using a high sensitivity troponin T (TnT) test we investigated whether an APR occurs in ACS only in the presence of ischemic myocardial damage.
Methods: In 85 patients with ACS C-reactive protein (CRP), serum amyloid A (SAA), fibrinogen, thrombin antithrombin III complexes (TAT) and kallikrein were determined vs. high sensitive TnT (0.02 ng/ml) initially and 2 d later vs. 45 patients with stable angina pectoris and 42 controls.
Results: In stable angina pectoris, markers of inflammation and coagulation were slightly elevated (p < 0.05). Initially in ACS elevations of CRP to 1.2 ± 0.3 mg/dl, SAA to 4.8 ± 2.6 mg/dl and fibrinogen to 448 ± 21 mg/dl (all p < 0.01 vs. controls) were found followed by a significant APR (p < 0.01).In the subgroup of TnT positive ACS patients, an APR with increased CRP (4.1 ± 1.3 mg/dl), SAA (20.4 ± 8.3 mg/dl), and fibrinogen (641 ± 45 mg/dl) was detectable (all p < 0.05 vs. TnT negative patients). In contrast, patients without TnT release showed APR markers comparable to patients with stable angina pectoris.
Conclusion: Our findings demonstrate an association between myocardial injury in ACS and acute phase reaction as evidenced by several molecular markers. A highly sensitive TnT-test identified myocardial inury in about all patients with APR while a standard TnT cut-off (0.1 ng/ml) missed 32% of these patients. Thus, the APR in patients with ACS is strongly associated with at least minor ischemic myocardial damage and prior findings of an APR independent from myocardial injury are probably based on less sensitive troponin tests. 相似文献
68.
Alloantibody against new platelet alloantigen (Lapa) on glycoprotein IIb is responsible for a case of fetal and neonatal alloimmune thrombocytopenia
下载免费PDF全文
![点击此处可从《Transfusion》网站下载免费的PDF全文](/ch/ext_images/free.gif)
69.
70.
Kaemmerer H Fratz S Braun SL Koelling K Eicken A Brodherr-Heberlein S Pietrzik K Hess J 《The American journal of cardiology》2004,94(6):825-828
A high percentage of cyanotic adults (37%) with cyanotic congenital cardiac disease (CCD) presented with depleted iron stores (13 of 52) or latent iron deficiency (6 of 52), even in a CCD center in which cyanotic patient phlebotomy is mostly avoided. In many of these patients, hypochromia and microcytosis was frequent, whereas hyperchromia and macrocytosis were relatively common.Furthermore, 50% of patients presented with hyperhomocysteinemia, possibly related to folate or B vitamin deficiencies, which may increase red blood cell size and color, explaining the lack of microcytosis and hypochromia in many cyanotic patients with iron deficiency. 相似文献