Synthesis of type I and III collagen, expression of fibronectin and matrix metalloproteinases-1 and -13 in hernial sac of patients with inguinal hernia

Since many years the importance of a weakness of the soft tissue for the development of hernias is discussed controversially. The tensile strength of the tissue is supposed to depend largely on the varying proportion of type I collagen with its high tensile strength and the immature type III collagen. Their relation is regulated by several collagenases, mainly matrix metalloproteinases-1 and -13 (MMP-1 and MMP-13), whereas fibronectin plays a key role for the adherence of cells within the extracellular matrix. The aim of this study was to investigate whether an alteration in type I and type III collagen synthesis, amounts of MMP-1 and MMP-13 and the expression of fibronectin were associated with the development of inguinal hernia. We analysed the hernial sac of patients with indirect (n = 9) and direct (n = 7) inguinal hernias and peritoneum in controls (n = 7) by immunohistochemistry and Western blot analysis. The results showed that the ratio of relative amount of I/III collagen was markedly decreased in patients with either indirect or direct hernias as compared with controls (p < 0.001) with a concomitant increase in type III collagen synthesis. MMP-13 was expressed neither in the hernial sac nor in the peritoneum of the controls, but the positive reactions of MMP-1 were found in the surface of the subserosa of the hernial sac in patients with indirect or direct hernias without any difference compared to controls. Furthermore, the relative amount of fibronectin in patients with either indirect or direct hernias is not significantly different from controls (p > 0.05). In regard to the known alterations of the collagen metabolism in fascia and skin of hernia patients the changed collagen I/III ratio with its increase of type III collagen in hernial sacs support the presence of a systemic disturbance of collagen metabolism. The absence of changes of the expression of collagenases (MMP-1, MMP-13) and the constant levels of fibronectin underline the central role of collagen synthesis for the development of indirect or direct hernias.     

空勤与地勤人员硒代谢平衡的研究

目的：为了解空勤与地勤人员的硒摄入,吸收和排泄量,以及硒代谢的差异,进一步做好空勤与地勤人员的营养保障,方法：用双盘法在３ｄ自由进食条件下对３６名空勤人员进行了硒代谢平衡的研究,并以３６名地勤人员作为对照,结果：空勤组的硒摄入量（５９．８５μｇ．ｄ＾－１）尿硒排泄量（３０．８４μｇ．ｄ＾－１）,表现吸收量（３９．２７μｇ．ｄ＾－１）和全血硒（９８．９１ｎｇ．ｍｌ＾－１）均高于地勤组（４５．９６μｇ     

Beauveriolides, specific inhibitors of lipid droplet formation in mouse macrophages, produced by Beauveria sp. FO-6979

Beauveria sp. FO-6979, a soil isolate, was found to produce inhibitors of lipid droplet formation in mouse peritoneal macrophages. A new compound beauveriolide III was isolated along with a known compound beauveriolide I from the fermentation broth of the producing strain by solvent extraction, ODS column chromatography, silica gel column chromatography and preparative HPLC. Beauveriolides I and III caused a reduction in the number and size of cytosolic lipid droplets in macrophages at 10 microM without any cytotoxic effect on macrophages.     

手术方式对老年性胃癌根治术预后的影响

目的　重点讨论手术方式对老年性胃癌根治术预后的关系。 方法　 回顾分析 1995年 1月～2 0 0 0年 1月共收治的 83例 6 5岁以上老年性胃癌根治术随访 3年 ,分析手术方式和心肺合并症的关系及其对死亡的影响。 结果　 三年后U区癌心肺合并症死亡 2 6例 (2 6 / 83,31 1% ) ,M区癌心肺合并症死亡 2例 (2 / 83,2 4 % ) ,L区癌心肺合并症死亡 8例 (8/ 83,9 6 % ) ,M区和L区共计 10例 (10 / 83,12 % )。两组 (U区与M +L区 )死亡率相比有显著差异 ,P <0 0 1。 结论　U区开胸手术易引起心肺合并症 ,是老年性胃癌根治术后死亡率增高的主要原因。     

半红树药用植物杨叶肖槿的化学成分和药理作用研究进展

通过CA检索国内外相关文献，介绍半红树药用植物杨叶肖槿近20年来的化学成分和药理活性的研究进展。杨叶肖槿的主要化学成分为mansonones类化合物，黄酮和三萜，其中mansonones类化合物是杨叶肖槿特有的一类天然产物，药理实验表明其具有抗肿瘤活性，杨叶肖槿具有多种药理和生理活性，是一种很有开发潜力的药用植物。     

南昌链霉菌生物合成梅岭霉素种子制备优化

以参数相关理论为指导,优化南昌链霉菌生物合成梅岭霉素种子制备工艺。结果表明,培养20～35h后,pH7.30～7.50,总糖<0.35%,pmv>10%,溶磷<100μg/ml,菌丝分化启动有利于提高梅岭霉素的发酵效价。     

反相高效液相色谱法测定发酵液中梅岭霉素

目的:建立了发酵液中梅岭霉素的定量测定方法。方法:反相高效液相色谱法,Hypersil-C_(18)柱(4 mm×125mm,5μm),流动相为乙腈-水(78:22),流速1.2mL·min~(-1),检测波长238 nm,室温测定。结果:在1O.2～163.2μg·mL~(-1)范围内梅岭霉素浓度与峰面积呈良好线性关系,r=0.999 7,回归方程为Y=25.44X+2.72。样品的日间 RSD≤1.8％,高中低浓度样品的日内RSD≤2.5％(n=8)。平均回收率为96.9％(n=8,RSD<3.0％)。结论:本方法快速、准确、简便、稳定,为定量分析提供了可靠的依据。     

多媒体人参生产智能专家系统在人参栽培中的应用

介绍了多媒体人参生产智能系统在人参栽培中的应用。通过对人参栽培中的水、肥、土、病、虫、草害进行图像及文本的采集、数字转化、专家推理，达到计算机对作物形态特征自动提取和智能诊断的目的，对提高人参栽培水平起到专家的指导作用。