Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence

Virulence and double-stranded RNA (dsRNA) profiles of 44 isolates of Fusarium virguliforme were compared. When grouped according to dsRNA profiles, isolates with large dsRNAs were significantly (P≤0.05) less virulent than isolates without dsRNAs. High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. The new viruses were most closely related to a group of unclassified viruses that included viruses of F. graminearum and Phlebiopsis gigantea and are related to members of the family Totiviridae.     

ApoE基因多态性与慢性心血管疾病及患者血脂水平的相关性研究

目的分析载脂蛋白E(ApoE)基因多态性与慢性心血管疾病及患者血脂水平的相关性。方法采用基因芯片分析系统及生化分析仪,检测1 414例慢性心血管疾病患者(实验组)和374例健康者(对照组)ApoE基因多态性及血脂水平。结果实验组和对照组ApoE基因型分布构成比比较差异有统计学意义(P0.05),对照组E2/3基因型构成比高于实验组。实验组总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)水平明显高于对照组,对照组高密度脂蛋白胆固醇(HDL-C)水平明显高于实验组,差异有统计学意义(P0.05)。E2/3基因型实验组患者HDL-C水平明显高于E3/4基因型患者,而E3/4基因型患者TC、LDL-C水平明显高于E2/3基因型患者,差异有统计学意义(P0.05)。不同ApoE基因型在脑梗死、脑出血、高血压、冠心病、2型糖尿病、脂肪肝等患者中的构成比有所差异,E3/4基因型在脑梗死、脑出血、高血压、冠心病、2型糖尿病、脂肪肝患者中所占比例高于E2/3基因型,差异有统计学意义(P0.05)。结论 ApoE基因多态性可能是导致个体血脂水平差异的重要原因之一,也是导致慢性心血管疾病发生、发展的危险因素。     

血栓弹力图对脑梗死患者抗血小板治疗的指导价值及阿司匹林抑制率对脑微出血的影响

目的 探讨血栓弹力图对脑梗死患者抗血小板治疗的指导价值及阿司匹林抑制率对脑微出血的影响。方法 回顾性分析2019年4月至2021年7月于阳江市人民医院治疗的88例脑梗死患者的临床资料。所有患者均接受阿司匹林抗血小板治疗,7 d后,利用血栓弹力图对血小板抑制率及有关参数进行检测,按照检测结果将阿司匹林反应低下的患者设置为A组(23例),无阿司匹林反应低下的患者设置为B组(65例)。改用氯吡格雷对A组患者持续抗血小板治疗7 d后,再次检测血小板抑制率。然后依据阿司匹林血小板抑制率分为良好组(血小板抑制率超过75%)、一般组(血小板抑制率为50%～75%)及无效组(血小板抑制率不足50%),分析阿司匹林抑制率对脑微出血的影响。结果 88例患者经阿司匹林干预后,42例(47.73%)恢复良好,23例(26.14%)恢复一般,23例(26.14%)反应低下,有效率为73.86%(65/88)。23例阿司匹林反应低下者改服氯吡格雷后,19例(82.61%)恢复良好,3例(13.04%)恢复一般,1例(4.35%)反应低下,有效率为95.65%(22/23)。A组治疗7 d后花生四烯酸(AA)诱导途...     

人GST-PTEN融合蛋白的原核表达和纯化

目的构建人第10号染色体上磷酸酶和张力蛋白同源缺失的基因(phosphatase and tensin homology deleted on chromosome ten,PTEN)原核表达质粒,使其在原核细胞中高效表达并进行纯化。方法将全长片段插入原核表达载体pGEX-4T-1,构建重组子pGEX-4T-1-PTEN,转化BL-21感受态细胞。经异丙基硫代-β-D-半乳糖苷(IPTG)诱导表达PTEN融合蛋白的可诱导性表达,通过SDS-PAGE电泳、Western印迹分析证实蛋白表达的特异性。并用谷胱甘肽-S-转移酶(glutathione-Stransferase,GST)亲和层析对融合蛋白进行纯化。结果成功构建了原核表达载体pGEX-4T-1-PTEN,并将PTEN融合蛋白的成功表达,通过SDS-PAGE电泳、Western印迹分析,证实了蛋白表达的特异性。并对蛋白进行了纯化,获得了GST-PTEN融合蛋白的纯品。结论成功表达、纯化了GST-PTEN融合蛋白,为进一步研究PTEN蛋白的功能及其与其它功能性蛋白的相互作用研究奠定了基础。     

血小板输注无效患者体内血小板反应性抗体特异性调查研究

目的探讨血小板输注无效患者体内血小板反应性抗体的发生频率及其特异性。方法对48名血小板输注无效患者,利用MACE法筛查其血清中的血小板反应性抗体,进一步用PAK12诊断试剂MAIPA法鉴定血小板HPA抗体特异性。结果血小板输注无效患者中血小板反应性抗体的总检出率为50%(24/48),其中同种HLA抗体发生率为39.6%(19/48),占所有抗体检出的79.2%,同种HPA抗体的检出率为29.2%(14/48),而其中有64.3%(9/14)是与HLA抗体同时存在。检出的HPA抗体特异性有抗-HPA-3a(n=2),-5a(n=1),-5b(n=1),-2b(n=1),-4b(n=1);同时HPA抗体中有78.6%(11/14)是针对GPⅡb/Ⅲa,Ⅰa/Ⅱa和/或Ⅰb/Ⅸ的多反应性抗体。女性患者中检出抗体的比例55.2%(16/29)高于男性患者42.1%(8/19),但差异没有显著性统计学意义。结论血小板输注无效患者中血小板同种抗体以HLA抗体多见,但HPA抗体并不像之前报道的罕见。与高加索人群PTR患者血小板同种反应性抗体以抗-HPA-5b,-1b为主不同,研究发现中国人群PTR患者HPA同种抗体以HPA-3,-5系统抗体多见,同时检出了HPA-4b,-2b抗体。     

复方聚乙二醇电解质散在妇科手术病人术前肠道准备中的应用

[目的]观察复方聚乙二醇电解质散在妇科手术病人术前肠道准备中的应用效果,探讨其护理措施。[方法]将150例妇科择期手术病人随机分为两组,观察组78例应用复方聚乙二醇电解质散进行术前肠道清洁准备,对照组72例口服硫酸镁及常规灌肠。比较两组病人肠道清洁度、肠道清洁过程中不良反应发生情况。[结果]观察组病人肠道清洁度优于对照组(P<0.05);观察组病人肠道清洁过程中发生不良反应为8.9 7%,对照组为19.44%,观察组明显少于对照组(P<0.05)。[结论]复方聚乙二醇电解质散用于妇科手术病人术前肠道清洁效果优于口服硫酸镁及常规灌肠,且不良反应少。     

留置膀胱引流管家庭护理现状调查分析与护理对策

目的 探讨留置膀胱引流患者家庭护理现状,为开展留置膀胱引流患者家庭护理健康教育提供依据.方法 对留置膀胱引流管的患者进行家庭护理状况问卷调查.结果 被调查对象53.7%对膀胱引流护理知识的不了解,92.5%认为留置引流管对其生活造成极大的不便,66.6%会尽量避免甚至拒绝参加一般的社交活动;对引流管护理知识的需求率为100%.结论 开展引流管家庭护理知识宣教很有必要,宣教的方式应采取多样的形式,既有操作的示范,还应有书面的指导.     

精神科约束带制作及应用方法的改良

目的 探讨精神科改良的新型约束带及应用方法在约束患者中的应用效果,为安全、有效约束提供参考.方法 在约束带的制作和应用方法上进行改进,应用于408例患者,观察、记录约束不良反应的发生和约束时间,并与实施前(418例)进行比较.结果 实施后约束引起的不良反应及约束时间较实施前显著减少(均P＜0.01).结论 我院约束带制作及应用的改良,加强了护理人员的工作责任心,减少了约束不良反应,缩短了约束时间,减少了患者的不舒适感,提供了更安全、有效、人性化的护理.     

Proteomic analysis of overweight/obesity and related abnormal glucose and lipid metabolism caused by phlegm-dampness retention

ObjectiveTo investigate the proteomic characteristics of overweight/obesity and related abnormal glucose and lipid metabolism caused by phlegm-dampness retention to identify related biomarkers.MethodsSeventy-one subjects were enrolled in the study. We assessed blood glucose, blood lipids, body mass index (BMI), and phlegm-dampness pattern, which was confirmed by a traditional Chinese medicine clinician. Of the participants, we included healthy participants with normal weight (NW, n = 23), overweight/obese participants with normal metabolism (ONM, n = 19), overweight/obese participants with pre-diabetes (OPD, n = 12), and overweight/obese participants with marginally-elevated blood lipids (OML, n = 17). Among them, the ONM, OPD, and OML groups were diagnosed with phlegm-dampness pattern. The data-independent acquisition (DIA) method was first used to analyze the plasma protein expression of each group, and the relevant differential proteins of each group were screened. The co-expressed proteins were evaluated by Venn analysis. The pathway analyses of the differential proteins were analyzed using Ingenuity Pathway Analysis (IPA) software. Parallel reaction monitoring (PRM) was used to verify the differential and common proteins in each group.ResultsAfter comparing ONM, OPD, and OML groups with NW group, we identified the differentially expressed proteins (DEPs). Next, we determined the DEPs among OPD, OML, and ONM groups. Using Venn analysis of the DEPs in each group, 24 co-expressed proteins were screened. Two co-expressed proteins were verified by PRM. IPA analysis showed that pathways including LXR/RXR activation, acute phase response signaling, and FXR/RXR activation were common to all three groups of phlegm-damp overweight/obesity participants. However, the activation or inhibition of these pathways was different among the three groups.ConclusionParticipants with overweight/obesity have similar proteomic characteristics, though each type shows specific proteomic characteristics. Two co-expressed proteins, VTN and ORM1, are potential biomarkers for glucose and lipid metabolism diseases with overweight/obesity caused by phlegm-dampness retention.