双标记原位杂交法检测间期细胞BCR/ABL融合基因

目的 :应用双标记原位杂交方法检测 BCR/ ABL融合基因。方法 :BCR基因探针用地高辛标记 ,碱性磷酸酶显色 ;ABL基因用3 H- d ATP标记 ,核子乳胶放射自显影。结果 :检测 9例初治的慢性粒细胞白血病 ( CML)病人均为阳性 ,阳性细胞比例为 93% ;检测 CML来源的 K5 62细胞株阳性细胞占 98.8% ;正常人假阳性率为 0 .75 %。结论 :该方法简便、快速 ,适用于 CML微小残留病的检测     

非小细胞肺癌患者外周血CD4^＋CD25^＋T细胞和TGF—β1的相关性分析e

目的：探讨非小细胞肺癌患者外周血中CD4^＋CD25^＋T细胞和TGF—β1（血清转化生长因子β1）的相关性及意义。方法：分别采用流式细胞技术和ELISA法检测40例非小细胞肺癌患者外周血中CD4^＋CD25^＋T细胞数量及TGF—β1的表达水平。结果：非小细胞肺癌患者外周血中CD4^＋CD25^＋T细胞和TGF-β1的表达水平明显高于对照组（P〈0．05）。肺癌患者外周血中CD4^＋CD25^＋T细胞与TGF—β1的表达成正相关。结论：非小细胞肺癌患者外周血中CD4^＋CD25^＋T细胞数量的增多可能诱导TGF—β1表达水平的增高。     

Routine contralateral orchiopexy for children with a vanished testis.

AIM: The aim was to determine whether routine contralateral orchiopexy is justified in a child with a vanished testis from intrauterine torsion. METHODS: A retrospective study of thirty-one consecutive boys with a vanished testis who underwent contralateral orchiopexy with the sutureless technique over an eight-year (1995 - 2002 inclusive) period was carried out. Operative findings were analyzed. All patients have been followed to date and interviewed by telephone. RESULTS: Of the thirty-one patients, 22 had testicular abnormalities (71 %). Five patients (16 %) had abnormalities that could predispose them to metachronous torsion. These included bell clapper deformity (3), horizontal lie (1), and ectopic testis (1). Other abnormalities were abnormal epididymal-testicular fusion (2), hydrocele (2), and one testis did not show compensatory hypertrophy. CONCLUSIONS: Sixteen percent of patients had testicular abnormalities that could predispose them to metachronous torsion. There was no morbidity from the operation and no post-orchiopexy torsion on follow-up. Therefore, routine contralateral orchiopexy in a boy with a vanished testis appears to be safe and effective. A large multicenter trial should be done to investigate these preliminary findings. If confirmed, contralateral orchiopexy should be advocated.     

高度近视LASIK治疗中角膜瓣厚度的探讨

目的:探讨高度近视LASIK治疗中角膜瓣厚度问题。方法:142例(283眼)屈光度-9.00～-12.00 D的近视患者用日本NIDEK MK-2000全自动微型板层角膜切割刀切开角膜瓣,厚度71～184μm,激光切削方式一致。将角膜瓣厚度≤110μm分为Ⅰ组,72例(144眼);角膜瓣厚度>110μm分为Ⅱ组,70例(139眼)。对术前、术后屈光度、视力以 及剩余角膜基质床厚度进行比较。结果:术前平均角膜厚度:Ⅰ组为(531.76±30.28)μm(469～575μm),Ⅱ组为(550.66±24.45)μm(506～584μm),两者有明显差异(P<0.05)。术后剩余角膜基质床厚度:Ⅰ组为(333.01±3.30)μm(273～452μm),Ⅱ组为(309.71±31.41)μm(254～368μm),两者有明显差异(P<0.05)。术前屈光度两组分别为(-10.61±1.01)D及(-10.13±0.73)D,两组间无明显差异(P>0.05),术后7 d时分别为(+1.05±1.38)D及(+1.32±1.70)D,两组间也无明显差异(P>0.05),而术后3m Ⅰ组为(-0.13±0.94)D,Ⅱ组为(-1.06±0.96)D,两组有明显的差异(P<0.01)。术前、术后不论UCVA还是BCVA,两组无明显差异(P>0.05)。结论:治疗高度近视时70～110μm的角膜瓣的制作是合理的,并不影响术后视力,对防止术后屈光回退是有效的,对一个有经验的医生来说是可以很好完成的。     

肉鸡对不同形态锰源的生物利用率研究

目的 :　研究肉仔鸡对不同形态锰源的相对生物学利用率。方法 :　将 62 4只 1日龄肉公鸡随机分为 1 3组 ,分别饲不添加锰的基础饲粮 (对照组 )或以不同锰化合物添加 60、1 2 0、1 80 mg/ kg锰的试验饲粮 2 1 d。结果 :　肉鸡生长性能各指标和腿病发生率以及肉鸡的跖骨灰锰含量、心肌细胞线粒体中 Mn SOD活性均未受到添加锰源以及锰源与锰水平互作的显著影响 ,但受到添加锰水平的显著影响。肉鸡心肌含锰量和 Mn SOD m RNA水平均未受到添加锰源与锰水平互作的显著影响 ,但受到添加锰源及添加锰水平的显著影响。其中中等和强络合强度有机锰源的生物学利用率明显高于无机硫酸锰和弱络合强度有机锰源。结论 :　中等和强络合强度的复合氨基酸锰对肉仔鸡的生物学利用率明显优于无机硫酸锰和弱络合强度的蛋氨酸锰     

再生障碍性贫血患者血清sICAM—l和TGF—β1水平及其意义

目的　探讨再生障碍性贫血 (AA ,再障 )患者血清可溶性细胞间粘附分子 - 1(sICAM - 1)和转化生长因子β1(TGF - β1)水平及其意义 .方法　采用ELISA法检测再障 3 0例和骨髓增生异常综合征 (MDS) 15例 ,正常对照组 2 0例血清sICAM - 1和TGF - β1水平 .结果　慢性再障 (CAA)组sICAM - 1较正常组明显增高 (p <0 0 5 ) ,TGF - β1较正常组明显降低 (p <0 0 1) ;重型再障 (SAA)和MDS组sICAM - 1均较正常组显著增高 (p均 <0 0 1) ,TGF - β1均较正常组显著下降 (p均 <0 0 1) .SAA组sICAM - 1较CAA组显著增高 (p <0 0 5 ) ,MDS组与CAA组比较 ,sICAM - 1明显增高 (p <0 0 1) ,MDS组与SAA组比较 ,TGF - β1明显增高 (p <0 0 1) .动态观察 10例再障 ,随着病情的好转 ,sICAM - 1水平逐渐下降 ,TGF - β1水平趋于上升 ;当病情加重时 ,sICAM - 1上升 ,TGF - β1下降 .血清sICAM - 1和TGF - β1接近正常者疗效、预后好 .结论　高水平的sICAM - 1和低水平的TGF - β1可能参与再障造血干 /祖细胞衰竭的病理生理过程 ;动态检测sICAM - 1和TGF - β1水平 ,为再障患者监测病情、评价疗效、指导临床治疗及预后判断提供较为客观的指标     

骶后孔(八髎穴)的临床应用解剖学

目的 :为八穴的针灸推拿以及骶后神经和骶管麻醉提供解剖学依据。方法 :我们测量了 30例骶骨标本 ,将骶后中线定为Y轴 ,将通过两骶角的连线定为X轴 ,测定骶后孔中点至两轴的距离 ;并测量骶后孔的口径 ,骶后孔中点至相应骶前孔中点的间距以及每侧 1～ 2 ,3～ 4骶后孔中点间距。结果 :根据统计分析 ,我们确定了两种骶后孔定位方法 ,取得了 1～ 2 ,3～ 4骶后孔中点间距的数值 ;4对骶后孔口径的大小顺序是 :1孔 >2孔 >4孔 >3孔。结论 :两种定位方法可帮助医生对骶后孔进行更为准确的定位 ,避免一些给患者带来的损伤 ,可使一些医疗麻醉等措施得以成功实施 ,有助于提高临床疗效     

Lack of association of angiotensin I-converting enzyme gene polymorphism and premature myocardial infarction in Mauritian Indians

Eighty-five young Mauritian Indians, male survivors of premature myocardial infarction (MI) and thus belonging to a high risk group, were compared with 108 stringently selected controls for a possible association between premature MI and an insertion/deletion (I/D) polymorphism in the gene encoding angiotensin I-converting enzyme (ACE). The frequency of the D allele was 0.42 in the MI group and 0.43 in the control group, and thus no association between I/D polymorphism of ACE with susceptibility to early-onset MI was found in this population group. Other gene components of the renin-angiotensin system and lipid metabolism need to be explored to understand the genetic factors involved in causing MI at an early age.     

Developmental characteristics of topographic EEG in the newborn using an autoregressive model

Summary Autoregressive topographic EEG analysis was used to determine topographic EEGs of the total power in quiet and active sleep stages in 33 healthy premature infants of 34 to 40 weeks conceptional age. The developmental characteristics were also examined by simultaneously referring to the autoregressive pattern discrimination of topographic EEGs between different conceptional age groups in both sleep stages. Treating 10.24 seconds of EEG as one segment, the topographic EEG of 10 segments in each of the quiet and active sleep stages as well as their mean were obtained. In both sleep stages the results showed a small peak in total power in the frontal region and a large peak in the occipital region, but total power was greater in the quiet sleep. Total power decreased with increasing conceptional age. Topographic pattern discrimination between different conceptional age groups showed significant differences mainly in the frontal, temporal and occipital regions. It was concluded that regional differences in the development of EEG in premature infants could be clarified by means of topographic EEG analysis and the pattern discrimination method using the autoregressive model.This study was supported by a Scientific Research Grant (No. 02670450) from the Japanese Ministry of Education.