高效液相色谱法测定晕痛定胶囊中的川芎嗪含量

目的 建立晕痛定胶囊中川芎嗪的含量测定方法。方法 用甲醇提取,以Nova-Pak C18色谱柱(4.6 mm×250 mm,4μm)为固定相,甲醇-水(50∶50,v/v)为流动相,检测波长为282 nm。结果 川芎嗪在10～50μg·mL-1范围内呈良好的线性关系,r=0.9992;平均回收率为98.7％,RSD=1.5％;日内精密度是RSD=0.7％。结论 该方法能消除其他成分的干扰,准确可靠、重现性好,操作简便快速。     

苦参碱抑制Akt信号通路诱导结肠癌SW480细胞凋亡

目的:研究苦参碱对人结肠癌SW480细胞的促凋亡作用及其可能的分子机制。方法:噻唑蓝比色法(MTT)测定苦参碱对SW480细胞株增殖的影响;Hoechst33258染色检测苦参碱对SW480细胞凋亡的影响,流式细胞术检测细胞凋亡率;蛋白免疫印迹法(Western blot)检测苦参碱对SW480细胞中总蛋白激酶B(Akt),磷酸化蛋白激酶B(p-Akt),B细胞淋巴瘤-2(Bcl-2),Bcl-2相关X蛋白(Bax)表达的干预作用。结果:苦参碱(0.25,0.5,1.0,1.5,2.0 g·L~(-1))能浓度和时间依赖性地抑制SW480细胞增殖。作用24,48,72 h的半数抑制浓度(IC50)分别为1.72,1.08,0.67 g·L~(-1),Hochest33258染色和流式细胞术结果显示苦参碱能显著诱导细胞凋亡(P0.01),随着药物质量浓度的提高,细胞凋亡率逐渐上升。Western blot检测显示,SW480细胞加入苦参碱后,胞内总Akt的含量无显著变化,但p-Akt的生成被显著抑制,凋亡抑制性蛋白Bcl-2表达降低,促凋亡蛋白Bax表达增高(P0.05)。结论:苦参碱能够诱导SW480细胞凋亡,该药理作用可能与苦参碱对Akt信号通路的抑制有关。     

中药指纹图谱数据库的研究

目的:研制中药指纹图谱数据库(DTCMF).方法:DTCMF采用Visual FoxPro(VFP)6.0编程语言编制程序.数据库包括色谱、光谱、电泳和DNA指纹谱以及X射线衍射、热分析谱等.结果:DTCMF可独立正常运行在目前常见的所有操作系统中,数据信息量大,检索系统完善,维护与更新便捷.结论:DTCMF具有通用性、实用性及易用性,为中药品质评价提供帮助与参考.     

Sensory Ataxic Guillain-Barré Syndrome with Dysgeusia after mRNA COVID-19 Vaccination

Guillain-Barré syndrome (GBS) has occasionally occurred in people who have received coronavirus disease 2019 (COVID-19) vaccines. Dysgeusia is rare symptom of GBS. We herein report a rare case of sensory ataxic GBS with dysgeusia just after the second dose of the Pfizer-BioNTech COVID-19 vaccine. Although autoantibodies against glycolipids were not detected, immunotherapy with intravenous immunoglobulin and methylprednisolone pulse therapy effectively ameliorated the symptoms. Our report suggests that the COVID-19 vaccine may induce various clinical subtypes of GBS, including a rare variant with sensory ataxia and dysgeusia.     

苯甲酸复配中药抽提液的抑菌效果及刺激性

目的：观察苯甲酸复配中药抽提液、苯甲酸抑菌剂及单纯中药抽提液的抑菌效果及其刺激性。方法：采用琼脂扩散法和动物试验对3种抑菌剂进行抑菌效果测定和刺激性试验。结果：苯甲酸复配中药抽提液和苯甲酸抑菌剂对金黄色葡萄球菌、大肠埃希菌和白色假丝酵母的抑菌圈直径比单纯中药抽提液显著增加,苯甲酸复配中药抽提液对实验动物的刺激性低于苯甲酸抑菌剂。结论：苯甲酸复配中药提取液抑菌效果显著强于纯中药抑菌剂,其刺激性低于苯甲酸抑菌剂。     

超声、PET/CT及两者联合检测对恶性黑色素瘤淋巴结转移的临床价值分析

目的：探讨超声、正电子发射断层成像/计算机断层成像（positron emission tomography/computed tomography,PET/CT）及两者联合检测对恶性黑色素瘤（malignant melanoma,MM）淋巴结转移评估的准确性,指导临床MM患者淋巴结活检及清扫的术前评估。方法：收集41例MM住院患者临床资料,分析术前淋巴结相关影像资料、术后淋巴结病理结果及病理结果跟影像结果的相关性。结果：超声、PET/CT以及两者联合检测的敏感度分别是47.36%、73.68%、78.95%,特异度分别是59.09%,86.36%、50.00%,准确度分别是53.66%、80.49%、63.41%,阳性预测值分别是50.00%、82.35%、57.69%,阴性预测值分别是56.52%,79.17%、73.33%,正确指数分别是6.45%、60.04%、28.95%。结论：PET/CT在评估MM术前淋巴结是否转移方面具有重要价值,其准确度、特异度、阳性预测值、阴性预测值均高于超声及两者联合检测,当PET/CT检查淋巴结转移为阴性时,应结合超声两者联合综合判断,避免淋巴结...     

针灸治疗溃疡性结肠炎的临床观察

目的 观察针灸治疗溃疡性结肠炎的疗效。方法与结果 采用温针灸：下合穴,募穴为主,配合辨证取穴,内服中成药,治疗溃疡性结肠炎62例,总有效率91．94％,T细胞亚群治疗后也接近正常。结论 显示本法有促进人体免疫系统内环境趋于平衡的作用。     

Prevention of surgical adhesions with gelatine sealing sheet in a canine adhesion model

Open in a separate window OBJECTIVESAlthough reoperation has been increasingly performed in cardiovascular surgery in recent years, preventing surgical adhesions remains an unsolved complication. Therefore, this study aimed to investigate whether gelatine sealing sheets are more effective than fibrin sealing sheets in preventing surgical adhesions.METHODSBilateral femoral arteries of 20 beagle dogs under general anaesthesia were pricked with syringe needles, and gelatine and fibrin sealing sheets were applied on the bleeding points to make canine adhesion models. The femoral artery was harvested after 4 and 12 weeks to evaluate adhesion formations. The adhesive grade was quantified by scoring the area and strength of adhesion tissues. Histological staining was performed to examine the structural features of surgical adhesions.RESULTSSignificantly fewer macroscopic adhesions were observed with gelatine sealing sheets than those with fibrin sealing sheets at 4 and 12 weeks postoperatively. Microscopically, CD3+ T lymphocytes at 4 and 12 weeks postoperatively in gelatine sealing sheets were significantly lower than those in fibrin sealing sheets. Microvessel density determined by CD34 at 4 and 12 weeks postoperatively in gelatine sealing sheets was also significantly lower than those in fibrin sealing sheets.CONCLUSIONSThe gelatine sealing sheets are more effective than the fibrin sealing sheets in preventing surgical adhesions. These findings suggest that the gelatine sealing sheet may help prevent adhesions and thus be a therapeutically effective biomaterial in vascular surgery.     

晚期非小细胞肺癌患者个体化治疗的新突破

在世界范围内,肺癌位居所有癌症致死的首位,且其中大部分为非小细胞肺癌.尽管大量有关含铂化疔或联合其他药物的临床研究不断涌现,非小细胞肺癌患者的预后仍然差强人意.晚期非小细胞肺癌患者的5年生存率约为15%,在过去几年中未有明显提高.目前,肺癌治疗领域的主要成就在于靶向治疗的出现,例如针对表皮细胞生长因子受体(EGFR)靶点的吉非替尼和厄罗替尼,以及针对抗血管内皮细胞生长因子(VEGF)的贝伐单抗,均广泛运用于临床.同时,关于棘皮动物微管相关蛋白样/间变淋巴瘤激酶(EML4-ALK)融合基因的研究使得肺癌的治疗又有了新的进展.该文主要对晚期非小细胞肺癌患者个体化治疗中的药物如EGFR抑制剂、EML4-ALK融合基因抑制剂进行综述.     

新鲜羊膜移植治疗翼状胬肉及严重睑球粘连56例临床观察

目的:观察新鲜羊膜移植对翼状胬肉及严重睑球粘连临床疗效的影响。方法:采用新鲜羊膜移植治疗翼状胬肉及严重睑球粘连56例73只眼,其中翼状胬肉37例49只眼,各种原因导致的大面积睑球粘连19例24只眼。翼状胬肉患者中复发性翼状胬肉为11例,初发性翼状胬肉为38只眼。手术均在显微镜下完成。结果:术后随访6~18个月,平均为12个月,93.2%(5/73)羊膜移植手术均一次成功,临床上均未见新鲜羊膜植片急性排斥反应。翼状胬肉患者除3只眼2月后复发外,其余全部均一次性治愈;严重睑球粘连24眼中,22只眼恢复了眼球的运动功能,2只眼术后3个月再次发生睑球粘连,但粘连面积较术前明显减小。结论:新鲜羊膜移植是治疗翼状胬肉及睑球粘连的有效方法。