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11.
小鼠扩张囊胚颗粒法玻璃化冷冻保存技术研究 总被引:8,自引:2,他引:6
在25℃室温下,用EFS30玻璃化溶液,对小鼠扩张囊胚进行颗粒法玻璃化冷冻保存。结果,胚胎在EFS30溶液中平衡1min后滴入液氮中,解冻后的胚胎发育率达100%,囊胚孵化率达76%。而在10%乙二醇溶液中将胚胎预处理5min,再移入EFS30中平衡1min后冷冻保存的胚胎,解冻后的发育率和囊胚孵化率分别为98%和63%。上述2组的胚胎发育率与对照组无明显差异,而囊胚发育率均明显低于对照组(P<0.01)。上述2组胚胎解冻后植入假孕小鼠子宫后的受体妊娠率分别为90%和81%,产出率分别为71%和58%,与对照组(61%)相比均无显著性差异(P>0.05)。 相似文献
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Two girls (a 5 year old and a 21 month old) experiencing mononucleosis syndrome with coincidental human herpesvirus (HHV)-7 and Epstein-Barr virus (EBV) infections are described. One patient had primary HHV-7 infection and reactivated EBV infection. The other had primary HHV-7 and EBV infections. These cases indicated that HHV-7 is capable of inducing infectious mononucleosis-like illness. Multiple herpesvirus infection in one of the patients also suggests that interaction among herpesviruses can occur in vivo. The consequence of this interaction may have clinical implications. 相似文献
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We report a case of solitary fibrous tumor of the thyroid gland in a 51‐year‐old man with pre‐existing right recurrent laryngeal nerve paralysis from a congenital tracheoesophageal fistula repair as an infant. The left thyroid lobe was enlarged and soft. Fine needle aspiration biopsy via ultrasound guidance demonstrated a hypercellular aspirate composed of spindle cells with bland nuclear morphology. Given this histomorphology and immunohistochemical profile, the diagnosis of solitary fibrous tumor was considered and confirmed following left thyroid lobectomy. Laryngoscope, 2009 相似文献
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CY. Chieng A. Davies D. Lowe F. Bekiroglu O. Khattak A. Schache R. Shaw S.N. Rogers 《The British journal of oral & maxillofacial surgery》2021,59(3):303-311
The prevalence of oral squamous cell carcinoma (OSCC) in the elderly is expected to increase by nearly a third in the next decade. Its management in older patients is potentially more challenging due to their pre-existing medical comorbidities, frailty, reduced life expectancy, and social issues. The aim of this retrospective review was to report on treatment given to patients aged 75 years and over, case mix, and survival. All patients aged 75 years and over who were diagnosed with OSCC in Merseyside between 1 January 2007 and 31 December 2016, and treated with either curative or palliative intent, were included. Their hospital notes were reviewed. Fisher’s exact test and Kaplan-Meier analysis were used for data analysis. There were 236 patients (median (IQR) age 81 (78-86) years); 67% were treated curatively and 33% palliatively. Factors associated with palliation included older age, advanced tumour stage, cognitive impairment, and residence in a nursing or residential home. Of the 165 patients who were offered curative treatment, six (4%) declined due to personal or family reasons. Overall survival for palliative patients was 12% at one year and 7% at two years, whereas for patients treated curatively it was 74% at one year, 56% at two years, and 34% at five years. Patients over 85 years of age were less likely to have composite free flaps and postoperative radiotherapy. Perioperative mortality was 2.6%. Improvements in surgical techniques and perioperative management have enabled clinicians to offer treatment with curative intent to older frail patients, and with careful case selection outcomes can be very good. 相似文献
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