In vitro blocking activity and biological effect of IgG (7S-antibodies) isolated from spleen cells and serum of mice developing Rauscher's leukemia

Immunoglobulins covering the surface of leukemic cells in the initial stage of Rauscher's leukemia in vivo and detectable in eluates from spleen cells of C57BL/6 and BALB/c mice, and also eluates of serum 7S-antibodies, when tested in vitro, block the cytotoxic effect of isologous and homologous 19S-antibodies against the group-specific antigen of mouse leukemic cells. The blocking antibodies (7S-IgG) isolated from both sources possess similar biological activity by stimulating the development of leukemia in experiments in vivo.Department for the Control of Virus Preparations, L. A. Tarasevich Government Institute for the Standardization and Control of Medical and Biological Preparations. Laboratory of Oncogenic Viruses, Moscow Research Institute of Virus Preparations. (Presented by Academician of the Academy of Medical Sciences of the USSR V. M. Zhdanov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 78, No. 12, pp. 55–58, December, 1974.     

黏液瘤病毒对大鼠体内胶质瘤作用的研究

目的 探讨黏液瘤病毒对大鼠动物模型体内胶质瘤细胞的作用.方法 采用立体定向法向SD大鼠颅内注射C6细胞建立大鼠额叶胶质瘤模型,明确成瘤后,随机分组,以立体定向方法往瘤腔内注射黏液瘤病毒(MV),5-FU,MV+ 5-FU及灭活黏液瘤病毒(DV),观察不同组别间大鼠体重、肿瘤大小、GFAP表达、Akt表达情况.结果 SD大鼠注射C6细胞后,额叶可见胶质瘤生长.成瘤后瘤腔内注射MV、5-FU及MV+ 5-FU,肿瘤的生长较注射DV减慢,并有缩小趋势,GFAP表达较少.MV组及MV+ 5-FU组PI3k、Akt及mTOR表达较DV组及5-FU组下降.结论 立体定向法注射C6细胞可以建立稳定的胶质瘤模型.MV可以通过调节PI3K-Akt-mTOR通路相关基因的表达,从而增加化疗药物对动物模型体内肿瘤细胞的生物学活性.     

缺氧诱导因子1α通过上调Snail促进HepG2肝癌细胞上皮-间充质化

目的上皮-间充质化（epithelial-mesenchymal transition,EMT）是实体瘤原发灶癌细胞获得转移能力的基础。缺氧诱导前列腺癌、肾癌、卵巢癌的EMT过程已得到证实,缺氧诱导因子1α（hypoxia inducible factor 1 alpha,HIF-1α）在这些过程中发挥重要作用。但是HIF-1α和肝癌细胞EMT之间的关系目前并不清楚。本文探讨HIF-1α在肝癌EMT中的作用。方法利用可调控HIF-1α表达的肝癌HepG2Tet-on-HIF-1α细胞系,在排除缺氧其他反应干扰的情况下研究HIF-1α在肝癌细胞EMT过程中的作用和机制。结果过表达HIF-1α促进HepG2肝癌细胞EMT,下调HIF-1α表达可以抑制HepG2肝癌细胞EMT。HIF-1α促进EMT相关转录因子Snail的表达。结论 HIF-1α通过上调Snail来促进HepG2肝癌细胞EMT。     

Role of serotoninergic structures in coordination of electric activity in the gastroduodenal complex

Myoelectric activity in various portions of the stomach and duodenum in normal and after vagotomy and intravenous injection of serotonin adipinate was studied in acute experiments on cats. Vagotomy disturbed coordination of myoelectric activity in the stomach and duodenum. Intravenous injection of serotonin adipinate restored coordination and increased myoelectric activity in the stomach, particularly, in the pylorus. Under these conditions the pacemaker of myoelectric activity in the gastric corpus and autonomic regulation of the duodenum were preserved. Intravenous injection of serotonin adipinate most significantly changed myoelectric activity in the pylorus. Myoelectric activity in the cardia increased after vagotomy against the background of serotonin adipinate. Our findings suggest that serotoninergic structures maintain functional heterogeneity of digestive organs and coordinate their interrelationships.     

Temperature topography of the brain cortex: Thermoencephaloscopy

Summary Thermoencephaloscopy (TES) - a new method of functional imaging of the cerebral cortex by its infrared radiation was advanced and developed since 1984. Improved thermovision and image processing techniques allow 2D, contact-free, dynamic and non-invasive recording of background and evoked cortical activity through an unopened skull. Activated (heated) and deactivated (cooled) zones of the cerebral cortex are revealed. The temporal resolution of TES is 40 msec (25 maps/sec), the spatial resolution is up to 70 × 70 microns/pixel. The diameter of the smallest recordable active region of the cortex is 200–300 microns. The minimal time needed for a session used for averaging of 4–9 responses varied from 40 sec up to 18 min. TES allows to detect the position, size and sequence of operation of precisely located specific cortical zones, and to measure their dynamics before, during and after sensory and direct cortical stimulation, motor acts and conditioning (associative learning). TES-effects were recorded in rats, rabbits, cats, monkeys and humans. Waves were found spreading over the cortex with a speed of up to 30 mm/sec along trajectories specific for the sensory modality and the site of stimulation. Some pathological processes in the brain are detectable by TES: experimental tumours and epileptic foci. There are many sources for local heating: neural activity, local metabolism of units, local cerebral blood flow and thermoconductivity in the activated zones of the cortex. Thermoencephaloscopy is a dynamic, non-invasive, contact-free method with a relatively high temporal and spatial resolution and sensitivity. It can be a useful tool in basic neuroscience and medicine.