Highly Drug-Resistant Salmonella enterica Serovar Indiana Clinical Isolates Recovered from Broilers and Poultry Workers with Diarrhea in China

Highly drug-resistant Salmonella enterica serovar Indiana became the most common serovar in broilers with diarrhea in China over the course of this study (15% in 2010 to 70% in 2014). While most S. Indiana isolates (87%, 384/440) were resistant to 13 to 16 of the 16 antibiotics tested, 89% of non-S. Indiana isolates (528/595) were resistant to 0 to 6 antibiotics. Class 1 integrons and IncHI2-type plasmids were detected in all S. Indiana isolates, but only in 39% and 1% of non-S. Indiana isolates.     

Octreotide-modified and pH-triggering polymeric micelles loaded with doxorubicin for tumor targeting delivery

A multifunctional mixed micelle was assembled for drug targeting delivery by combining two newly synthesized amphiphilic polymers, which were octreotide-polyethylene glycol-monostearate (OPMS) and N-octyl-N-succinyl-O-carboxymethyl chitosan (OSCC), respectively. The mixed micelle was designed to be characterized with long circulation, somatostatin receptors (SSTR)-mediated endocytosis and pH sensitivity. A series of assembling proportions of OPMS and OSCC was tested to reveal the effect of compositions on the functions. The particle size, zeta potential, drug loading and critical micelle concentration were examined. The dialysis test indicated a pH-triggering release behavior of the doxorubicin-loaded mixed micelle (DLMM), and faster release in acidic media (pH 4.0-6.0) in response to the protonation of carboxyl group. In addition, the PEG segments could efficiently protect the mixed micelle from plasma protein adsorption in vitro, and the DLMM composed of 20% OPMS and 80% OSCC provided the longest residence time after intravenous injection in rats in vivo. Due to SSTR mediated endocytosis, the significantly higher uptake of DLMM was observed in the tumor cells (SMMC-7721), compared with that in the normal cells (CHO) without SSTR expression. All the results suggested that the mixed micelle with multifunctional characteristics could be used as an effective approach for tumor treatment.     

脑梗塞合并心衰、酸碱平衡紊乱的治疗体会

目的:探讨脑梗塞合并心力衰竭、酸碱平衡紊乱的临床治疗体会。方法:对36例脑梗塞合并心力衰竭、酸碱平衡紊乱的病例治疗经过及结果进行回顾分析,总结脑梗塞病人在治疗过程中出现脑水肿与酸碱平衡紊乱、心力衰竭相互加重病人病情及治疗措施。结果:36例经适当治疗后,存活32例,神经功能恢复状况较为理想。结论:脑梗塞合并心力衰竭、酸碱平衡紊乱的病人,在治疗原发病的过程中,必须兼顾各种合并症,以及合并症可能造成的危害,才能达到治疗的目的。     

组织透明化技术在神经退行性疾病中的应用研究进展

组织透明化技术是一种快速发展的新兴组织学技术,将大块组织、单个器官、啮齿动物全身以及人类大型标本,通过一系列物理化学原理与方法进行处理,在保持其完整性的同时快速实现高度光学透明,为高通量地获取生物组织三维结构信息提供了重要的工具.结合现代光学成像技术和荧光标记技术,可显著提高成像深度和图像对比度,已成为经典组织学技术有...     

样本量估计与检验效能分析（六）

所谓单因素多水平设计,是指试验中仅涉及一个具有尼个水平（k≥3）的试验因素,未对其他任何重要非试验因素进行有计划的安排。本文向读者介绍单因素多水平设计一元定量资料与结果变量为二值变量的单因素多水平设计一元定性资料的样本含量与检验效能估计。     

42岁男性主动脉弓离断1例报告

主动脉弓离断是一种罕见的、婴幼儿期死亡率高的严重复杂畸形,能长大成人更为罕见。一位42岁男性高血压患者,经主动脉CT血管造影检查证实,降主动脉起始部血管与主动脉弓中断,邻近脊柱旁、肋间及胸背部皮下软组织内见多个扭曲紊乱血管团,并于降主动脉起始水平见数支血管汇入降主动脉CT血管造影。经降压治疗后,症状好转出院。本研究报道给我们的启示:对年轻高血压患者要仔细听诊,一旦前胸背部听到杂音,及时行CTA检查,避免漏诊和误诊。     

Optimal body fat percentage cut-offs for obesity in Chinese adults

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Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina

Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22 kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody.