Thiolated poly(aspartic acid)-functionalized two-dimensional MoS2, chitosan and bismuth film as a sensor platform for cadmium ion detection

In this work, a sensitive electrochemical platform for determination of cadmium ions (Cd²⁺) is obtained using thiolated poly(aspartic acid) (TPA)-functionalized MoS₂ as a sensor platform by differential pulse anodic stripping voltammetry (DPASV). The performance of the TPA–MoS₂-modified sensor is systemically studied. It demonstrates that the TPA–MoS₂ nanocomposite modified sensor exhibits superior analytical performance for Cd²⁺ over a linear range from 0.5 μg L⁻¹ to 50 μg L⁻¹, with a detection limit of 0.17 μg L⁻¹. Chitosan is able to form a continuous coating film on the surface of the GC electrode. The good sensing performance of the TPA–MoS₂-modified sensor may be attributed to the following factors: the large surface area of MoS₂ (603 m² g⁻¹), and the abundant thiol groups of TPA. Thus, the TPA–MoS₂-modified sensor proves to be a reliable and environmentally friendly tool for the effective monitoring of Cd²⁺ existing in aquacultural environments.

In this work, a sensitive electrochemical platform for determination of cadmium ions (Cd²⁺) is obtained using thiolated poly(aspartic acid) (TPA)-functionalized MoS₂ as a sensor platform by differential pulse anodic stripping voltammetry (DPASV).     

Synthesis of stable-isotope-labeled N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide and N-(3-dimethylaminopropyl)-N′-ethylurea

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) is a carbodiimide coupling reagent commonly used for the preparation of amides from carboxylic acids and amines. Because of initial concerns regarding the genotoxicity of EDC and its use in GMP syntheses at Bristol Myers Squibb, the quantitation of residual EDC and its by-product N-(3-dimethylaminopropyl)-N′-ethylurea (EDU) by liquid chromatography–mass spectrometry (LCMS) impurity analysis was required. These analyses required the use of stable-isotope-labeled EDC and EDU to serve as internal standards. To meet this need, stable-isotope-labeled EDC 9 and EDU 10 were prepared from [1,2-¹³C₂] ethylene glycol and [¹³C,¹⁵N] potassium cyanide in overall yields of 6% and 8%, respectively.     

不同剂量右美托咪定对老年糖尿病足截趾患者全身麻醉苏醒期的影响

目的对比分析不同剂量右美托咪定对老年糖尿病足截趾患者全身麻醉苏醒期的影响。方法选取2017年4月至2018年8月北京市丰台区南苑医院收治的102例拟行截趾术治疗的老年糖尿病足患者作为研究对象,并按照随机数表法将其随机分为低剂量组(51例)和高剂量组(51例),其中低剂量组患者于全身麻醉时静脉缓慢泵注0. 4μg/kg的盐酸右美托咪定注射液,高剂量组患者于全身麻醉时静脉缓慢泵注0. 8μg/kg的盐酸右美托咪定注射液,对比观察两组患者诱导麻醉前、拔管时、拔管后5 min、拔管后10 min的平均动脉压(MAP)、心率(HR)、血氧饱和度(SpO_2),以及苏醒时间及拔管时间。结果诱导麻醉前、拔管时、拔管后5 min、拔管后10 min,两组患者MAP、HR及SpO_2对比,P均0.05,差异无统计学意义;低剂量组患者的苏醒时间为(14. 02±5. 87) min、拔管时间为(16. 55±5. 26) min,明显低于高剂量组患者的苏醒时间(18. 16±6.29) min、拔管时间(22. 73±6.84) min,两组对比,P均0.05,差异具有统计学意义。结论老年糖尿病足患者在截趾术中应用低剂量右美托咪定辅助麻醉,可有效缩短患者苏醒时间及拔管时间,维持患者苏醒期的血流动力学稳定性,安全性较高,具有临床推广价值。     

中国成年双生子高脂血症的分布特征

目的描述中国双生子登记系统(CNTR)成年双生子高脂血症的分布特征, 初步探索遗传和环境因素对高脂血症的影响。方法研究对象来自CNTR在全国11个项目地区募集的双生子, 纳入成年且具有高脂血症信息的69 130名(34 565对)双生子进行分析。采用随机效应模型描述高脂血症的人群、地区分布特征。分别计算不同卵型双生子的高脂血症同病率, 估算遗传度。结果研究对象年龄为(34.2±12.4)岁。双生子人群高脂血症患病率为1.3%(895/69 130)。男性、年长、城镇、已婚、大专及以上文化程度、超重、肥胖、体力活动不足、当前吸烟和曾经吸烟、当前饮酒和曾经饮酒人群中高脂血症患病率较高。双生子对内分析发现, 同卵双生子高脂血症同病率为29.1%(118/405), 高于异卵双生子的18.1%(57/315), 差异有统计学意义(P<0.05)。在不同年龄、地区及性别分层中, 同卵双生子同病率仍呈现高于异卵双生子的趋势。进一步同性别双生子对内分析发现, 在北方组和女性组中, 高脂血症遗传度分别为13.04%(95%CI:2.61%～23.47%)、18.59%(95%CI:4.43%～3...     

接种天花疫苗对猴痘病毒感染的保护作用研究进展

2022年,猴痘疫情从区域的地方性流行扩大至全球暴发,并被WHO列为国际关注的突发公共卫生事件。由于正痘病毒之间的基因序列具有高度相似性且正痘病毒可诱发交叉反应型抗体,接种天花疫苗可能会影响猴痘病毒感染诱导的免疫应答水平及感染者的健康结局。分析天花疫苗接种对猴痘病毒感染的保护作用将有助于确定疫情防控策略和措施。因此,本文通过了解既往猴痘疫情中天花疫苗的接种情况及其健康结局,综述天花疫苗接种、免疫应答情况和临床数据之间的关联性,为猴痘疫情的预防控制策略提供科学证据。