菊花总黄酮含药血清对干眼细胞模型AR、NF-κB磷酸化蛋白及TGF-β1表达的影响＊

目的 观察菊花总黄酮在体外条件下对泪腺上皮细胞中雄激素受体（Androgen Receptor，AR）的调控作用及对下游的核因子κB（Nuclear Factor kappa B，NF-κB）、转化生长因子-β1（transforming growth factor-beta1，TGF-β1）的表达影响。方法 以过氧化氢（H₂O₂）诱导泪腺上皮细胞凋亡，建立细胞模型，设立无雄激素培养空白对照组、含雄激素培养对照组、无雄激素培养黄酮治疗组。以MTT法摸索含药血清最佳干预加入量；免疫印迹实验（Western Blot）以及实时荧光定量PCR法（Quantitative Real-Time PCR，qPCR）检测泪腺上皮细胞中AR、NF-κB及TGF-β1的表达情况；并应用雄激素受体阻断剂氟他胺进行AR阻断后再次检测上述指标 。结果 MTT法检测后计算得出含药血清干预细胞的最终浓度为13.2%；无雄激素培养黄酮治疗组及含雄激素培养对照组中AR、NF-κB及TGF-β1的表达增强，与空白对照组对比有显著差异（P<0.01）；无雄激素培养黄酮治疗组中NF-κB表达量比含雄激素培养对照组表达量低，两者间的差异有统计学意义。氟他胺及含药血清干预后，无雄激素培养黄酮治疗组及含雄激素培养对照组中AR及NF-κB的表达未见提高，无统计学意义。结论 菊花总黄酮对泪腺上皮细胞可能存在拟雄激素效应，从而使AR、NF-κB表达增加，并通过上调TGF-β1的表达，可能起到在泪腺局部的抗炎作用。     

中国西部地区医生的职业倦怠与工作家庭冲突的相关研究

目的 探讨中国西部地区医生的职业倦怠及其与社会支持、工作家庭冲突的关系.方法 采用职业倦怠量表中国版(CMBI)、社会支持以及工作影响家庭和家庭影响工作问卷对611名西部地区的医生进行了调查研究.结果 中国西部地区的医生中,14.1％处于情感衰竭状态,49.4％处于人格解体状态,33.1％感到成就感降低；27.3％的医生轻度倦怠,43.7％中度倦怠,3.3％高度倦怠.工作5～10年和工作11 ～20年的医生情感衰竭较为严重；本科和硕士学历的医生人格解体最严重；高级和中级职称的医生人格解体水平较高；二级医院医生人格解体程度最高,三级医院医生的成就感最高.Logistic回归分析提示社会支持对职业倦怠有保护作用,家庭工作冲突则为危险因素.结论 中国西部地区医生职业倦怠现状较为严重,工作与家庭之间的平衡和良好的社会支持有助于缓解医生的职业倦怠.     

美国儿科药品审批政策概述

简要介绍美国儿科药品审批政策并进行深入分析,总结其成功经验,为建立适合我国的儿科药品审批政策提供参考.     

HIMF在低氧性肺动脉高压的研究进展

低氧诱导促有丝分裂因子(hypoxia-induced mitogenic factor,HIMF)是一种相对分子质量为9 400,富含半胱氨酸的分泌蛋白,最初在鼠的肺组织用卵清蛋白诱发哮喘模型时发现的.HIMF由111个氨基酸残基组成,其结构分为3个组分:①N末端信号序列；②中间可变区；③高度保守的C末端信号序列.已经证实用短发夹环RNA敲减低氧性肺动脉高压大鼠的HIMF基因,能够阻止平均肺动脉压升高、降低肺血管阻力、减轻右心室肥厚以及改善慢性缺氧引起的血管重塑作用.本文主要就HIMF在低氧性肺动脉高压的作用及机制研究作一综述.     

Isolation and characterization of 23 microsatellite loci in the Chinese sucker (Myxocyprinus asiaticus)

Chinese sucker (Myxocyprinus asiaticus) is a second class state-protected animal in China. In this study, we developed twenty-three polymorphic microsatellite loci in Chinese sucker. The observed and expected heterozygosity ranged from 0.292–0.958 to 0.423–0.900, respectively. The polymorphic information content ranged from 0.356 to 0.869, with a mean of 0.710. These microsatellite loci are expected to be useful for further studies of genetic diversity, population genetic structure, and assessments of the artificial propagation release effect of Chinese sucker.     

基于MSP430F149单片机的心电信号数据采集系统的设计与研究

目的设计一种低成本的便携式心电信号数据采集系统，实现长时间对心电信号进行采集与存储。方法对采集的心电信号进行放大、滤波、电平提升和模数转换后存储于Flash存储器，再通过液晶显示器显示实时心电波形。结果本系统可实现对心电信号进行长时间的数据采集和记录。结论本系统对于临床和患者应用都具有一定的实用价值。     

先天性心脏病经特殊途径封堵并发下腔静脉异位引流

先天性心脏病并发下腔静脉异位引流发病率低,是罕见的先天性心脏血管畸形,需要选择特殊路径建立轨道,增加了介入封堵术的难度及风险。我院2000年开展先天性心脏病介入封堵术,总计发现3例并发下腔静脉异位引流的患者,其中2例成功行介入封堵术,1例放弃介入手术。我们总结经验及体会,探讨经特殊途径行介入封堵术的可行性及安全性。     

“120”出诊中急性左心衰的救治体会

目的探讨“120”出诊中急性左心衰竭的救治措施。方法回顾分析34例急性左心衰竭的出诊病例，均经过了病情快速判断、心理安抚、吸氧、辅助端坐体位、心电监护、急救药物治疗、正确转运等处理，少部分患者进行了电除颤、心肺复苏治疗。结果34例患者经过急救处理后病情均不同程度的得到了缓解，安全到达医院者31例，急救有效率91．18％。结论急性左心衰竭是常见的危急重症。提高急救意识、熟练抢救方法、医护担架工积极配合、完善的抢救设备是提高抢救有效率的保证。另外，与患者及家属的积极有效沟通也至关重要，可有效减少医患纠纷。     

The Therapeutic Role of VEGF‐Expressing Muscle‐Derived Stem Cells in Acute Penile Cavernosal Injury

IntroductionTraumatic penile injury is one of the urological emergencies. Surgery and conservative management are major treatment methods but are always accompanied by many complications.AimTo investigate the feasibility of repairing cavernous tissues in acute rabbit penile cavernosal injury model with vascular endothelial growth factor (VEGF)‐expressing muscle‐derived stem cells (MDSCs).MethodsMDSCs were isolated and transfected with hVEGF₁₆₅ lentiviral gene vector in vitro. The expression of VEGF was confirmed using enzyme‐linked immunosorbent assay (ELISA), Western blot, and real‐time polymerase chain reaction (PCR) analyses. After animal models were constructed, animals were randomly divided into four groups, which were administrated with MDSCs/VEGF, MDSCs/vector, MDSCs, and normal saline, respectively. A month later, magnetic resonance imaging (MRI) and intracavernosal pressures (ICP) were performed on the animals. Then penile tissues were harvested and assayed with Western blot and immunohistochemistry.Main Outcome MeasuresReal‐time PCR, Western blot, ELISA, immunohistochemistry, MRI, and ICP were performed in our experiments.ResultsThe expression of VEGF significantly increased in the VEGF‐expressing MDSCs group compared with those in the MDSCs/vector and MDSCs groups. VEGF protein expression in the injury sites of cavernous tissues were significantly higher in the MDSCs/VEGF group compared with those in other three groups. Immunohistochemical staining showed that α‐smooth muscle actin‐positive cells, von Willebrand factor‐positive cells and capillary density markedly increased in the MDSCs/VEGF group. Animals receiving MDSCs/VEGF showed a significant improvement in cavernosal contractile function and structural repair.ConclusionsThe transplantation of VEGF‐expressing MDSCs could repair the actuely injured cavernous tissue. We believed that it could be a novel therapeutic strategy for acute rabbit penile cavernosal injury. An G, Ji C, Wei Z, Chen H, and Zhang J. The therapeutic role of VEGF‐expressing muscle‐derived stem cells in acute penile cavernosal injury. J Sex Med 2012;9:1988–2000.