全文获取类型
收费全文 | 5055篇 |
免费 | 468篇 |
国内免费 | 295篇 |
专业分类
耳鼻咽喉 | 137篇 |
儿科学 | 60篇 |
妇产科学 | 85篇 |
基础医学 | 578篇 |
口腔科学 | 62篇 |
临床医学 | 679篇 |
内科学 | 651篇 |
皮肤病学 | 78篇 |
神经病学 | 217篇 |
特种医学 | 190篇 |
外国民族医学 | 2篇 |
外科学 | 484篇 |
综合类 | 905篇 |
一般理论 | 1篇 |
预防医学 | 374篇 |
眼科学 | 116篇 |
药学 | 532篇 |
10篇 | |
中国医学 | 328篇 |
肿瘤学 | 329篇 |
出版年
2024年 | 10篇 |
2023年 | 68篇 |
2022年 | 236篇 |
2021年 | 311篇 |
2020年 | 247篇 |
2019年 | 158篇 |
2018年 | 166篇 |
2017年 | 177篇 |
2016年 | 142篇 |
2015年 | 261篇 |
2014年 | 281篇 |
2013年 | 300篇 |
2012年 | 409篇 |
2011年 | 451篇 |
2010年 | 326篇 |
2009年 | 233篇 |
2008年 | 241篇 |
2007年 | 212篇 |
2006年 | 200篇 |
2005年 | 211篇 |
2004年 | 167篇 |
2003年 | 141篇 |
2002年 | 140篇 |
2001年 | 85篇 |
2000年 | 82篇 |
1999年 | 103篇 |
1998年 | 49篇 |
1997年 | 58篇 |
1996年 | 35篇 |
1995年 | 35篇 |
1994年 | 33篇 |
1993年 | 17篇 |
1992年 | 27篇 |
1991年 | 27篇 |
1990年 | 23篇 |
1989年 | 24篇 |
1988年 | 16篇 |
1987年 | 14篇 |
1986年 | 16篇 |
1985年 | 8篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1980年 | 4篇 |
1979年 | 5篇 |
1976年 | 4篇 |
1975年 | 7篇 |
1973年 | 4篇 |
1972年 | 7篇 |
1971年 | 7篇 |
1967年 | 3篇 |
排序方式: 共有5818条查询结果,搜索用时 0 毫秒
21.
Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays 总被引:12,自引:0,他引:12
Sha J Zhou Z Li J Yin L Yang H Hu G Luo M Chan HC Zhou K;Spermatogenesis study group 《Molecular human reproduction》2002,8(6):511-517
We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1-4 weeks. Among these genes, 101 were identified as critically related to testis development and possibly to spermatogenesis since they were found in both human and mouse testes, and expressed differentially at different stages of testis development. Of the 101 development-related genes, 59 full-length cDNAs have been sequenced previously, while the full-length cDNAs of the other 42 genes have not been published. We have obtained 11 full-length sequences of the 42 genes and deposited them in the GenBank. The conserved testis development-related genes found in both human and mouse testes may include genes that are likely to be involved in testicular functions, especially spermatogenesis, thus providing a basis for further functional characterization of the genes in mouse models. 相似文献
22.
In this study, a human adult testis cDNA microarray was constructed and hybridized with (33)P-labeled human adult testis, embryo testis and sperm cDNA probes, respectively. A novel alternative splice variant of BRDT gene, named BRDT-NY, presumably involved in testicular function was cloned. It was expressed 3.96-fold more in human adult than embryo testis and also expressed in human spermatozoa. Similarly, RT-PCR revealed a differential expression pattern of this gene in human adult testes and fetal testes. The full length of BRDT-NY was 3438 bp and contained a 2883 bp open reading frame, encoding a 960-amino-acid protein. Sequence analysis showed that it has two bromodomains in N-terminal of the protein. Multiple tissue RT-PCR results showed that BRDT-NY was exclusively expressed in testis. mRNA expression of BRDT-NY gene was deleted in some azoospermic patients' testes. These experiments suggested that BRDT-NY gene may have an important role in the process of spermatogenesis and may be correlated with male infertility. 相似文献
23.
中国狂犬病毒疫苗株(5aG)糖蛋白基因在痘苗病毒中的表达及免疫效果观察 总被引:1,自引:0,他引:1
将克隆到的中国狂犬病毒疫苗株(5aG)的糖蛋白基因重组到痘苗病毒TK区,并在痘苗病毒P11启动子的控制下,构建了狂犬-痘苗重组病毒(VVaG)。经间接免疫荧光和Western免疫印染证明,重组病毒VVaG能良好地表达狂犬病毒糖蛋白,其分子量约为6600。用VVaG免疫小鼠,7d便可诱生较高的狂犬病毒中和抗体,21d达4169,并能100%保护狂犬病毒本毒株和国际标准攻击毒(CVS)的致死量攻击。 相似文献
24.
目的 探讨喉癌组织Bc1 2和Bax基因蛋白表达与喉癌发生、发展的关系。方法 采用免疫组化SP法测定 5例正常人声带组织和 73例喉癌组织标本中Bc1 2和Bax基因蛋白表达。结果 喉癌组织中Bc1 2基因蛋白表达阳性检出率 45 .2 % ,喉癌组织中Bax基因蛋白达阳性检出率 6 0 .3% ,与正常人相比 ,均无显著性差异 (P >0 .0 5 )。喉癌组织中Bc1 2及Bax基因蛋白的表达呈明显的负相关关系 ,r值 - 0 .71,P <0 .0 5。结论 喉癌组织中Bc1 2和Bax基因蛋白表达的失调可能与喉癌的发生、发展有关。 相似文献
25.
Kai Dong Mengjun Shen Guanqun Ju Sha Han Zhijun Wang Kaining Lu Dongliang Xu 《European urology》2021,79(2):283-289
BackgroundPartial nephrectomy or angioembolisation is commonly used for sporadic renal angiomyolipomas (RAMLs) with high RENAL scores, but there is a risk of reduced renal function, postoperative complications, and recurrence.ObjectiveTo describe a new technique for off-clamp laparoscopic evacuation of sporadic RAMLs with high RENAL scores that promotes maximal renal function maintenance and low postoperative complication and lesion recurrence rates.Design, setting, and participantsA retrospective cohort of patients undergoing off-clamp laparoscopic evacuation for sporadic RAMLs with RENAL scores ≥9 from January 2013 to June 2018 was included.Surgical procedureWe highlighted the curettage, suction, packing, and binding (CSPB) technique, a new off-clamp retroperitoneoscopic evacuation technique for sporadic RAMLs.MeasurementsDemographics, preoperative, intraoperative, and postoperative outcomes were assessed.Results and limitationsA total of 141 cases were included. The median (interquartile range [IQR]) tumour size was 7 (6.2–8.2) cm. The median (IQR) RENAL score was 10 (9–11). The median (IQR) operative time was 80 (65–125) min, with a median (IQR) estimated blood loss of 130 (90–362.5) ml. Conversion to neither open surgery nor standard laparoscopy occurred. The warm ischaemia time was zero for all cases. Postoperatively, 13 minor complications (Clavien grade 1) were recorded. No blood transfusions were reported. The glomerular filtration rate did not change significantly from preoperative period to 12-mo follow-up. Recurrence did not occur at the median follow-up period of 48 (36–60) mo. The retrospective design and lack of a control group are limitations of this study.ConclusionsOff-clamp retroperitoneoscopic tumour evacuation using the CSPB technique is feasible, safe, and effective for treating complex sporadic RAMLs.Patient summaryWe report a curettage, suction, packing, and binding technique for off-clamp retroperitoneoscopic evacuation of sporadic renal angiomyolipomas that leads to complete lesion clearance, excellent renal function preservation, and minimal perioperative complications. 相似文献
26.
Wang Ben Deng Yu-xuan Yan Sha Xie Hong-fu Li Ji Jian Dan 《Lasers in medical science》2021,36(3):649-655
Lasers in Medical Science - Acne scarring is one of the most common facial skin disorders. The appropriate treatments for acne scars in patients with rosacea have not been studied. This study was... 相似文献
27.
Zhang Jing Zhang Yunqing Qin Jinglin Lu Sha Cai Wenying Li Jiahao Huang Huaiqiu Yang Sulian Xi Liyan 《Lasers in medical science》2021,36(1):147-152
Lasers in Medical Science - Onychomycosis is a fungal infection of the nail. The aim of this randomized controlled clinical trial was to compare the efficacy of 2940-nm Er:YAG laser treatment... 相似文献
28.
Sha Sha James A. Pearson Jian Peng Youjia Hu Juan Huang Yanpeng Xing Luyao Zhang Ying Zhu Hongyu Zhao F. Susan Wong Li Chen Li Wen 《Diabetes》2021,70(2):504
Toll-like receptor 9 (TLR9) is highly expressed in B cells, and B cells are important in the pathogenesis of type 1 diabetes (T1D) development. However, the intrinsic effect of TLR9 in B cells on β-cell autoimmunity is not known. To fill this knowledge gap, we generated NOD mice with a B-cell–specific deficiency of TLR9 (TLR9fl/fl/CD19-Cre+ NOD). The B-cell–specific deletion of TLR9 resulted in near-complete protection from T1D development. Diabetes protection was accompanied by an increased proportion of interleukin-10 (IL-10)–producing B cells. We also found that TLR9-deficient B cells were hyporesponsive to both innate and adaptive immune stimuli. This suggested that TLR9 in B cells modulates T1D susceptibility in NOD mice by changing the frequency and function of IL-10–producing B cells. Molecular analysis revealed a network of TLR9 with matrix metalloproteinases, tissue inhibitor of metalloproteinase-1, and CD40, all of which are interconnected with IL-10. Our study has highlighted an important connection of an innate immune molecule in B cells to the immunopathogenesis of T1D. Thus, targeting the TLR9 pathway, specifically in B cells, may provide a novel therapeutic strategy for T1D treatment. 相似文献
29.
30.
目的:构建一种太赫兹(THz)超材料传感方法用于microRNA-21(miRNA-21)的信号放大检测。方法:首先构建THz超材料传感方法,并用聚丙烯酰胺凝胶电泳及zeta电位验证方法的可行性;对传感器检测条件进行优化之后,对不同浓度的miRNA-21以及其他不同的miRNAs进行检测,并与其他microRNA检测方法进行比较;最后,对该传感器的回收率进行了评价。结果:在最优实验条件下,通过双链特异性核酸酶(DSN)循环识别与滚环扩增(RCA)的双重信号放大策略,该THz超材料传感器对靶标miRNA-21的响应范围为10 fmol/L至10 nmol/L,检测限为8.49 fmol/L。并且该传感器具有较好的特异性,具备了从多种miRNAs中识别靶标miRNA-21的能力,并且在商业化人血清样本中的回收率可达94.33%到115.33%。结论:该THz传感器可以实现靶标miRNA-21的高灵敏、高特异性检测,具备了在miRNA相关疾病无标记诊断与早期预警的潜力。 相似文献