Association of AGTR1 gene methylation and its genetic variant in Chinese farmer with hypertension: A case-control study

The objective was to determine the potential associations of the angiotensin II receptor type 1 (AGTR1) gene polymorphism, methylation, and lipid metabolism in Chinese farmers with hypertension.A case-control study was conducted in Wuzhi county of Henan province in China in 2013 to 2014. A total of 1034 local residents (35–74 years, 386 hypertensive cases, and 648 normotensive subjects) were enrolled in this study. Triglyceride (TG), total cholesterol (TC), high-density lipoprotein, and low-density lipoprotein were measured using automatic chemistry analyzer. The AGTR1 gene promoter methylation level was measured using quantitative methylation-specific polymerase chain reaction method. The single nucleotide polymorphism rs275653 was genotyped with TaqMan probe assay at an applied biosystems platform.The gender, body mass index (BMI), TG, TC, and family history of hypertension in the hypertension group were significantly higher than those in control group (P < .05). No significant difference was observed in the distribution of AGTR1 rs275653 polymorphism in the hypertension and controls (P > .05). The AGTR1 gene methylation in subjects carrying different genotypes was not significantly observed (P > .05). The logistic regression analysis found the AGTR1 gene methylation level was negative correlation with hypertension in the present study (odds ratio, 0.946, 95% confidence interval, 0.896–0.999) through adjusting for age, gender, BMI, education, smoking, alcohol drinking, fruit and vegetable intake, pickles intake, and family history of hypertension.The association of AGTR1 gene hypomethylation and essential hypertension was observed in Chinese farmers; no significant difference was observed in the distribution of AGTR1 rs275653 polymorphism.     

肾上腺良性无功能瘤39例临床分析

总结肾上腺良性无功能瘤３９例,其中３３例因健康查体或其他疾病就诊时发现,仅６例因腰腹不适或腹部包块而就诊。肾上腺内分泌激素检查均正常。肿瘤位于一侧肾上腺的３８例,双侧的１便。３０例病灶直径〉２．２ｃｍ的患者经治疗,病理结果：肾上腺皮质腺瘤２２例,蛹地腺髓性脂肪瘤４例,肾上腺节能细胞神经瘤２例,肾上腺囊肿１例,肾上腺神经鞘瘤１例。对肾上腺无功能瘤的诊断和治疗进行了讨论。     

Safety and efficacy of anti-EGFR monoclonal antibody (SCT200) as second-line therapy in advanced esophageal squamous cell carcinoma

Objective: The mainstay treatment of esophageal squamous cell carcinoma(ESCC) involves chemotherapy and immunotherapy. However, alternative therapies are required for patients who are refractory or intolerant to existing therapies.Methods: In this single-arm, multicenter, open-label phase Ib study, 30 patients received an intravenous infusion of SCT200, an antiepidermal growth factor receptor(EGFR) monoclonal antibody, 6.0 mg/kg once a week for 6 weeks, followed by 8.0 mg/kg once every 2 weeks u...     

Apaf－1在乳腺癌治疗中的作用及意义

乳腺癌的新辅助化疗不但可以降低临床分期、提升保乳率,还可以判断化疗药物的敏感性,并指导术后化疗用药,已逐渐成为乳腺癌综合治疗的首选。但仍有少数乳腺癌患者不能从新辅助化疗中受益,预测乳腺癌新辅助治疗的获益人群,是临床中亟待解决的问题。乳腺癌新辅助化疗后的细胞凋亡指数（apop-totic index,AI）是评估乳腺癌新辅助化疗疗效的关键因素,而Apaf－1作为凋亡途径的重要一员,在细胞凋亡中起到重要的作用。本文通过乳腺癌新辅助化疗后AI值的变化,并结合Apaf－1自身的结构、分布、在凋亡途径中的作用及在各肿瘤中的表达和化疗耐药性等角度,对Apaf－1在乳腺癌治疗中的作用及意义作一综述。     

Recombinant LH supplementation to recombinant FSH during induced ovarian stimulation in the GnRH-antagonist protocol: a meta-analysis

This study aims to compare the efficacy of recombinant LH (rLH) supplementation for ovarian stimulation in gonadotrophin-releasing hormone-antagonist protocol for IVF/intracytoplasmic sperm injection cycles. Search strategies included online surveys of databases. The fixed effects model was used for odds ratio (OR) and effect size (weighted mean difference, WMD). Five trials fulfilled the inclusion criteria. When the meta-analysis was carried out, advantages were observed for the LH supplementation protocol with respect to higher serum oestradiol concentrations on the day of human chorionic gonadotrophin administration P < 0.0001; WMD: 514, 95% CI 368, 660) and higher number of mature oocytes (P = 0.0098; WMD: 0.88, 95% CI 0.21, 1.54). However, these differences were not observed in the total amount of recombinant FSH (rFSH) administered, days of stimulation, number of oocyets retrieved, the clinical pregnancy rate per oocyte retrieval, the implantation rate and miscarriage rate. This result demonstrates that the association of rLH with rFSH may prevent any decrease in oestradiol after antagonist administration and that a significantly higher number of mature oocytes was available for laboratory work. Nevertheless, it failed to show any statistically significant difference in clinically significant end-points in IVF (implantation and pregnancy rates). Additional randomized controlled trials are needed to confirm these results further.     

Novel Amdoparvovirus Infecting Farmed Raccoon Dogs and Arctic Foxes

A new amdoparvovirus, named raccoon dog and fox amdoparvovirus (RFAV), was identified in farmed sick raccoon dogs and arctic foxes. Phylogenetic analyses showed that RFAV belongs to a new species within the genus Amdoparvovirus of the family Parvoviridae. An RFAV strain was isolated in Crandell feline kidney cell culture.     

人部分正常与肿瘤细胞株中neuritin表达的检测

目的:筛选neuritin高表达及低表达细胞株,为进一步探讨neuritin在不同组织细胞生长发育过程中的作用奠定基础.方法和结果:分别利用RT-PCR与免疫组化技术在mRNA水平及蛋白水平,检测了11种人类细胞株中neuritin的表达情况,发现不仅在多种正常细胞中有表达,而且在部分肿瘤细胞中也有表达.结论:成功筛选出了neuritin高表达及低表达细胞株.     

金昌队列人群非酒精性脂肪肝发病的影响因素分析

目的了解金昌队列人群非酒精性脂肪肝(NAFLD)发病的影响因素,为NAFLD的预防和控制提供科学依据。方法以金昌队列基线未患脂肪肝且符合纳入标准的20051人为研究对象,通过前瞻性队列研究及Cox回归分析探讨NAFLD发病影响因素,并用限制性立方样条法研究相关生化指标与NAFLD发病风险的剂量反应关系。结果NAFLD发病密度为42.37/1000人年,多因素Cox回归分析显示,职业为工人与技术人员(工人:HR=0.84,95%CI:0.70~0.99;技术人员:HR=0.73,95%CI:0.56~0.95)、饮茶(正在饮:HR=0.86,95%CI:0.78~0.94;过去饮:HR=0.52,95%CI:0.31~0.86)、体育锻炼(偶尔:HR=0.79,95%CI:0.68~0.91;经常:HR=0.60,95%CI:0.52~0.69)、体重较轻(HR=0.10,95%CI:0.05~0.22)、奶类及奶制品摄入>300 ml/d(HR=0.78,95%CI:0.71~0.87)、HBV感染(HR=0.77,95%CI:0.60~0.99)是NAFLD的保护因素;职业为内勤服务人员(HR=1.84,95%CI:1.46~2.31)、家庭人均月收入≥2000元(2000~元:HR=1.32,95%CI:1.04~1.66;≥5000元:HR=1.72,95%CI:1.11~2.66)、文化程度为本科及以上(HR=1.35,95%CI:1.03~1.76)、超重(HR=2.31,95%CI:2.08~2.55)、肥胖(HR=3.95,95%CI:3.42~4.56)、空腹血糖受损(HR=1.31,95%CI:1.17~1.47)、糖尿病(HR=1.53,95%CI:1.30~1.80)、TC升高(HR=1.37,95%CI:1.24~1.52)、TG升高(HR=1.79,95%CI:1.62~1.98)、HDL-C降低(HR=1.29,95%CI:1.14~1.45)、ALT升高(HR=1.13,95%CI:1.01~1.26)和高脂饮食(HR=1.24,95%CI:1.11~1.40)是NAFLD的危险因素,TC、TG、HDL-C、ALT、FPG与NAFLD发病呈良好的剂量反应关系。结论职业、文化程度、家庭人均月收入、饮茶、体育锻炼、BMI、FPG、血脂、ALT、HBV、饮食与NAFLD发病有关。     

Prognostic value of expression of p53, proliferating cell nuclear antigen, and c-erbB-2 in laryngeal carcinoma.

BACKGROUND: The molecular mechanisms in malignant transformation of laryngeal mucosa are unknown; many clinical and pathological factors affect prognosis. We investigated a possible correlation between overexpression of p53, proliferating cell nuclear antigen (PCNA), and c-erbB-2, and the clinicopathologic features of laryngeal squamous cell carcinoma (SCC) and clarify their prognostic value. MATERIAL/METHODS: Thirty-three men with laryngeal SCC participated; all underwent primary surgery or surgery plus radiotherapy between 1999 and 2004 at our department. Paraffin sections of laryngeal SCC were immunohistochemically stained for p53, PCNA, and c-erbB-2. RESULTS: Overall, p53 overexpression was found in 16 patients. There was no relation between p53 immunohistochemical staining and tumor region. PCNA immunostaining was significantly stronger in supraglottic tumors compared with glottic tumors. Immunostaining of c-erbB-2 was not different in either location. There was no relation between the T stage of the tumor and p53 and c-erbB-2 immunostaining. However, there was a statistically significant positive correlation between the PCNA staining and T stage (stronger staining was present in T3 and T4 stages than in T1 and T2 stages). CONCLUSIONS: We could not find a statistically significant correlation between p53, PCNA, and c-erbB-2 and lymph node status, histologic differentiation, and survival rate. We demonstrated only a statistically significant positive correlation between PCNA staining and T stage. These data suggest that overexpression of p53, PCNA, and c-erbB-2 is not prognostic in laryngeal SCC.     

C-erbB-2、MMP2在乳腺癌中的表达及相互关系探讨

目的 :探讨癌蛋白 (C erbB 2 )和基质金属蛋白酶 (MMP2 )在乳腺浸润性导管癌中的表达及相互关系。方法 :应用免疫组织化学S -P法对 5 6例乳腺浸润性导管癌的C erbB 2和MMP2的阳性表达进行检测分析。结果 :5 6例中有 37例 (6 6 % )C erbB 2过表达 ,39例 (6 9.6 % )MMP2过表达 ,C erbB 2和MMP2同时阳性表达者 32例(5 7 1% )。两者阳性率均随组织学分级升高而上升 ,但各级间未见明显差异 (P >0 .0 5 )。在转移组中 ,88.9% (2 4 /2 7)的病例C erbB 2过表达 ,85 .2 % (2 3/ 2 7)MMP2过表达 ,两者的过表达与转移呈正相关关系 (PC erbB 2 =0 .0 0 1,PMMP2 =0 .0 15 )。C erbB 2和MMP2阳性配对分析表明 ,两者间阳性表达互为正相关 (P <0 .0 0 5 ,r=0 .5 1)。结论 :在乳腺浸润性导管癌中 ,C erbB 2和MMP2的过表达提示转移增加 ,可以作为乳腺癌的预后指标。