Luminal non-specific cationic channels in cultured strial marginal cells of guinea pig and gerbil as determined by patch clamp technique.

Using primary cultures of marginal cells of stria vascularis from guinea pig and gerbil, ionic channels located on the luminal membrane were investigated by means of patch clamp technique. Recordings were performed in cell-attached and inside-out configurations. In cell-attached configuration, single channel activity was identified with a conductance of about 25 pS. I-V curve was linear. The probability of opening was increased upon depolarization. Up to 7 channels could be present in the same patch, indicating a rather high density. In inside-out configuration, the reversal potential was 0 mV, suggesting a non-specific cationic channel. These luminal non-specific cationic channels would allow the passive K+ efflux and Na+ influx across the apical membrane of marginal cells. This finding is consistent with the "one-pump" model of strial activity. The present study suggests that culture of strial marginal cells may be a suitable model for in-depth investigation of endolymph physiology.     

P300 in freely moving cats with intracranial electrodes.

Nine chronically implanted cats were used in order to study the event related potentials (ERPs) of the brain with the "omitted stimulus" paradigm. The cats were not restrained and not conditioned, could behave freely in a soundfree room. Due to a selective averaging method a P300 like of response component could be detected in the hippocampus, auditory cortex and reticular formation. The most robust and ample component was the N200-P300 wave complex of the hippocampus. The results are interpreted as follows: the P300 component in the cat brain has probably distributed generators with various frequency selectivities. The hippocampal ERP has a strong theta response component, whereas the auditory cortex and reticular formation have more marked 10 Hz responses.     

The application of dysarthria profile tests in ALS patients for the detection of speech disturbances

Dysarthria is an invalidating disability in ALS patients with motor neuron degeneration in the bulbar region. The methods to assess dysarthric disorders in ALS are seldom described in publications. This study was performed in 43 patients who had definite (n = 23) or probable (n = 20) ALS (of the bulbar group n = 15, of the limb group n = 28, mean age = 57.07 (range: 36-69 yr.)) according to WFN criteria. The method based on quantitative tests of dysarthria profile (by Robertson, 1986) was used and the results were compared with 37 age, sex-matched, healthy control subjects. Our study showed the existence of disturbances in all dysarthria profile tests which were of the statistic significance and more frequent as compared to the control subjects (p < 0.01). The analysis showed that quantitative assessment of some dysarthria profile tests (5 out of 8) might be useful in clinical practice to detect dysarthria in ALS patients. Using the dysarthria profile tests we also demonstrated that preclinical dysarthric processes occur among the limb ALS group.     

Retroperitoneal benign schwannoma. Report of a new case

The schwannoma is a tumor resulting from the Schwann cells of neural shwath, being its retroperitoneal localization quite unusual. This tumor is clinically unspecified and in most of the cases it originates symptoms coming from the compression of the close structures when its localization is retroperitoneal. Its diagnosis is quite often fortuitous being confirmed by anatomopathological study afterwards. The treatment is surgical radical exeresis with subsequent followup. We report a new case of this uncommon retroperitoneal pathology in a female patient showing a nonspecific clinic. Two years after the surgery she remains asymptomatic without any radiological evidence of recidive.     

Massive lymphatic involvement secondary to prostatic adenocarcinoma

Prostatic carcinoma may be diagnosed by the clinic manifestations or by the symptoms for locoregional disemination and distance metastasis. The lymphatic system is the first metastatic station, which is affected in a high percentage of cases. Event it may simulate lymphoproliferatives process and it si uncommon the lymphatic macroaffectation at first. In theses cases, the histologic and immunohistochemical study by the determination of prostatic specific antigen in lymph nodes can provice the diagnosis. Treatment of these tumors is palliative with hormonotherapy. Prognosis is bad with a low survival at five years.     

加强团队建设 培育团队精神

团队精神是现代医院精神的重要组成部分，是促进医院凝聚力、增强医院竞争力的精神力量。医院是人的集合，众多的人扮演不同的角色，从事各自不同的医疗活动，但又必须互相配合，去完成共同的目标，因而管理的效能往往并不是1+1=2，而是1+1>2或1+1<2。如果医院部门之间或者职工之间出现扯皮、摩擦，人为地造成大量的内耗，肯定1+1<2；真正科学的管理，正在于通过不断协调和整合，使医院形成一个紧密衔接、步调和谐的团队，创造出1+1>2的效能，这是团队精神最本质的要求。如何加强团队建设，培育团队精神，结合工作实际，谈几点看法。     

SARS病例的流行病学生存分析及对卫生检疫的影响

〔目的〕研究SARS病例生存时间的影响因素,并探讨对口岸卫生检疫的影响。〔方法〕对119例SARS病例进行描述性流行病学调查分析,运用统计学单因素方差分析、卡方检验、Kaplan-Meier和CoxRegession生存分析方法进行研究。〔结果〕119例SARS病例以发热(97.48%)和咳嗽(42.02%)为最常见始发症状,入院平均体温为38.28±0.86℃;平均住院时间是47.75d(95%的可信区间为43.50￣52.00d);男性与女性间平均住院时间的差异无统计学意义(P=0.1918);65岁(含65岁)以上年龄组及65岁以下年龄组死亡率分别为38.50%和4.72%,两者差异有极显著性(P<0.001)。〔结论〕SARS具有人群普遍易感性,以年龄对死亡的影响最为显著。对重点人群应加强口岸卫生检疫,完善口岸体温监测系统;检疫人员应做好个体防护。