Phenotypic and genotypic characteristics of Candida albicans isolates from bloodstream and mucosal infections

The interaction of Candida albicans with the host is of a complex nature involving fungal factors and host's response. In this study, we concentrated on the phenotypic expression of virulence attributes and genotypic characteristics of C. albicans isolates from two distinct clinical entities of candidiasis—blood stream and vaginal infections, and the possible role of these factors. Hence, we conducted a comparative in vitro assessment of virulence characteristics, including adhesion to epithelial cells and HaCat cell line, biofilm formation, aspartic proteinases and phospholipase activity of 20 C. albicans isolates from patients with C. albicans bloodstream infection and 22 isolates from patients with C. albicans vaginitis. Further, we studied the epigenetic phenotypic switching of the strains and their ploidy, by flow cytometry and CHEF techniques. These studies indicated that although no overall differentiation between the isolates of the two groups (bloodstream infection and vaginitis) could be demonstrated, several characteristics were more specific to one of the groups than the other. While the strains from vaginal infection had higher capacity to adhere, the strains from patients with bloodstream infection had higher activity of phospholipase. Differences were also noted in phenotypic switching, with the strains from bloodstream infection revealing primarily the “white” type colonies, known to be more virulent, and had higher DNA content. This study is unique considering the concurrent comparison of isolates from different clinical entities, at the phenotypic and genotypic level.     

Damon-Ⅲ矫治器治疗安氏Ⅱ类1分类错畸形患者硬组织变化的研究

目的探讨Damon-Ⅲ自锁托槽矫治技术治疗安氏Ⅱ类1分类错畸形患者治疗前后的硬组织变化。方法选择采用Damon-Ⅲ自锁托槽矫治技术和MBT矫治技术治疗的安氏Ⅱ类1分类错畸形患者各20例,均采用减数4颗第一前磨牙的治疗方案,未额外使用增强支抗的装置,治疗前后拍摄患者头颅侧位X线片并进行测量。结果2组患者治疗后,上下颌骨矢状向变化不明显,垂直向有增大的趋势,差异有统计学意义;2组患者牙性指标均有变化,但略有不同。结论Damon-Ⅲ自锁托槽矫治技术和MBT矫治技术均可快速高效地治疗安氏Ⅱ类1分类错畸形,后牙支抗消耗较少,但是Damon-Ⅲ自锁托槽矫治技术使患者的前牙转距丧失较多。     

糖基化恢复冷藏血小板的存活率

血小板冷藏会使血管假性血友病因子受体复合物(von Willebrand factor receptor complex)聚集成簇。巨噬细胞αMβ2 整合素结合在成簇复合物的GPIbα亚基,导致输注的冷藏血小板被快速清除。因此输注用血小板不能冷藏,但现在的室温保存方式也存在很大缺点。我们已证明αMβ2是一种凝集素,它能识别GPIbα的N-连接葡聚糖上暴露的β-N-乙酰葡萄胺。冷藏血小板的酶促半乳糖苷化阻止了αMβ2的这种识别,延长了有     

人本主义对艾滋病防控策略的影响

艾滋病是全球面临的一个重大公共卫生问题和社会问题。对艾滋病感染者和艾滋病病人的歧视,必然导致这部分人不敢公开到正规机构接受咨询检测和治疗,从而成为潜在的传染源,造成艾滋病的扩散蔓延。在艾滋病的防控策略中引入人本主义精神,能够营造全社会关注、支持与参与艾滋病防控的良好氛围,最终实现控制艾滋病的目标。     

Benzofuran Derivatives as Potent,Orally Active S1P1 Receptor Agonists: A Preclinical Lead Molecule for MS

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