全文获取类型
收费全文 | 16410篇 |
免费 | 1260篇 |
国内免费 | 33篇 |
专业分类
耳鼻咽喉 | 162篇 |
儿科学 | 336篇 |
妇产科学 | 393篇 |
基础医学 | 2147篇 |
口腔科学 | 404篇 |
临床医学 | 2052篇 |
内科学 | 3345篇 |
皮肤病学 | 166篇 |
神经病学 | 1325篇 |
特种医学 | 629篇 |
外科学 | 2535篇 |
综合类 | 234篇 |
一般理论 | 33篇 |
预防医学 | 1447篇 |
眼科学 | 213篇 |
药学 | 1329篇 |
中国医学 | 16篇 |
肿瘤学 | 937篇 |
出版年
2023年 | 133篇 |
2022年 | 209篇 |
2021年 | 503篇 |
2020年 | 288篇 |
2019年 | 497篇 |
2018年 | 556篇 |
2017年 | 393篇 |
2016年 | 439篇 |
2015年 | 424篇 |
2014年 | 637篇 |
2013年 | 746篇 |
2012年 | 1180篇 |
2011年 | 1272篇 |
2010年 | 693篇 |
2009年 | 603篇 |
2008年 | 982篇 |
2007年 | 993篇 |
2006年 | 955篇 |
2005年 | 912篇 |
2004年 | 872篇 |
2003年 | 694篇 |
2002年 | 673篇 |
2001年 | 234篇 |
2000年 | 235篇 |
1999年 | 188篇 |
1998年 | 149篇 |
1997年 | 126篇 |
1996年 | 87篇 |
1995年 | 87篇 |
1994年 | 100篇 |
1993年 | 74篇 |
1992年 | 120篇 |
1991年 | 130篇 |
1990年 | 119篇 |
1989年 | 90篇 |
1988年 | 59篇 |
1987年 | 90篇 |
1986年 | 98篇 |
1985年 | 84篇 |
1984年 | 75篇 |
1983年 | 66篇 |
1982年 | 47篇 |
1981年 | 36篇 |
1979年 | 66篇 |
1978年 | 51篇 |
1977年 | 44篇 |
1975年 | 43篇 |
1974年 | 48篇 |
1972年 | 46篇 |
1969年 | 41篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
Soos JM Polsky RM Keegan SP Bugelski P Herzyk DJ 《Clinical immunology (Orlando, Fla.)》2003,109(2):188-196
Natural antibodies to cytokines can be found in the sera of normal healthy individuals in the absence of specific immunostimulation. However, the function, impact, and purpose of natural antibody development have yet to be fully elucidated. Interleukin (IL)-18 is a cytokine that exerts proinflammatory activities and induces natural killer (NK) cell activity. Recombinant human IL-18 (rHuIL-18) is currently in development as a cancer immunotherapy. In this study, the presence of natural antibodies to IL-18 in the sera of normal humans and three nonhuman primate species was evaluated by electrochemiluminescence immunoassay (ECLIA). Of the human sera tested, 6 of 47 samples were positive for natural antibodies to IL-18. Of the nonhuman primate sera tested, 22 of 80 cynomolgus monkey samples, 4 of 31 rhesus monkey samples, and 2 of 20 chimpanzee samples were positive for natural antibodies to IL-18. Natural anti-IL-18 antibodies were neutralizing in 5 of 22 cynomolgus and 2 of 4 rhesus sera. None of the chimpanzee or human sera were able to neutralize IL-18 induction of interferon (IFN)-gamma in vitro. In vivo activity of rHuIL-18 was compared in IL-18 natural antibody-positive and -negative cynomolgus monkeys. The presence of natural antibodies to IL-18 did not alter rHuIL-18 systemic exposure levels, induction of neopterin, or induction of treatment-induced antibodies following intravenous administration of rHuIL-18. In conclusion, our data indicate that, as has been found with other cytokines, natural anti-IL-18 antibodies are relatively common. Moreover, natural anti-IL-18 antibodies do not appear to influence rHuIL-18 activity in vivo and are not predictive of a heightened immune response, suggesting that natural anti-IL-18 antibodies do not impact IL-18 therapy. Finally, our data suggest that the ability to detect natural anti-cytokine antibodies may be a useful measure of the adequacy of an assay for deployment in clinical trials. 相似文献
92.
93.
94.
Page D 《JEMS : a journal of emergency medical services》1994,19(8):83-5, 87
95.
Pluronic P-85 (poly(55)(oxypropylene)dipoly(s)(oxyethylene)) was used to form daunorubicin containing micelles. This new carrier was tested in vitro on sensitive and resistant ovarian cancer cell lines. Drug incorporation was measured by cytofluorometry and the cytotoxicity was measured by the tetrazolium formazan XTT assay. ID50 of 0.16 and 25 mu g/ml were obtained for conjugated and free daunorubicin respectively. The results obtained suggest that this approach may be used in combination with a chemotherapeutic agent to overcome multidrug resistance. 相似文献
96.
Klein C Page CE LeWitt P Gordon MF de Leon D Awaad Y Breakefield XO Brin MF Ozelius LJ 《Neurology》1999,52(3):649-651
Some patients with an 18p- syndrome show dystonia, and a focal dystonia gene has been mapped to chromosome 18p. The authors evaluated the extent of the deletion in three patients with an 18p- syndrome and dystonia using 14 DNA markers on 18p. A common deleted area, covering the DYT7 locus, places the putative dystonia gene between the telomere of 18p and D18S1104 (49.6 cM). Dystonia in these patients may be caused by haploinsufficiency of the DYT7 gene, a new dystonia gene on 18p, or may result from developmental brain anomalies. 相似文献
97.
98.
Laparoscopic Splenectomy 总被引:2,自引:0,他引:2
Splenectomy is a powerful tool for treatment of hematologic disease, with 70% to 90% of patients achieving long-term improvement. In recent years laparoscopic splenectomy has gained acceptance as a viable alternative to open splenectomy. This review summarizes the indications for laparoscopic splenectomy, the operative techniques, and the most recent results. Laparoscopic splenectomy is evolving and may become the standard operative method for the treatment of the problem spleen. 相似文献
99.
Serotonergic mediation of the effects of fluoxetine, but not desipramine, in the rat forced swimming test 总被引:3,自引:3,他引:0
Rationale: The forced swimming test (FST) is a behavioral test in rodents that predicts the clinical efficacy of many types of antidepressant
treatments. Recently, a behavior sampling technique was developed that scores individual response categories, including swimming,
climbing and immobility. Although all antidepressant drugs reduce immobility in the FST, at least two distinct active behavioral
patterns are produced by pharmacologically selective antidepressant drugs. Serotonin-selective reuptake inhibitors increase
swimming behavior, while drugs acting primarily to increase extracellular levels of norepinephrine or dopamine increase climbing
behavior. Distinct patterns of active behaviors in the FST may be mediated by distinct neurotransmitters, but this has not
been shown directly. Objectives: The present study examined the role of serotonin in mediating active behaviors in the forced swimming test after treatment
with two antidepressant drugs, the selective serotonin reuptake inhibitor, fluoxetine and the selective norepinephrine reuptake
inhibitor, desipramine. Methods: Endogenous serotonin was depleted by administering para-cholorophenylalanine (PCPA, 150 mg/kg, IP.) to rats 72 h and 48 h
prior to the swim test. Fluoxetine (10 mg/kg, SC) or desipramine (10 mg/kg, SC) was given three times over a 24-h period prior
to the FST. Behavioral responses, including immobility, swimming and climbing, were counted during the 5-min test. Results: Pretreatment with PCPA blocked fluoxetine-induced reduction in immobility and increase in swimming behavior during the FST.
In contrast, PCPA pretreatment did not interfere with the ability of desipramine to reduce immobility and increase climbing
behavior. Conclusions: Depletion of serotonin prevented the behavioral effects of the selective serotonin reuptake inhibitor fluoxetine in the rat
FST. Furthermore, depletion of serotonin had no impact on the behavioral effects induced by the selective norepinephrine reuptake
inhibitor, desipramine. The effects of antidepressant drugs on FST-induced immobility may be exerted by distinguishable contributions
from different neurotransmitter systems.
Received: 4 February 1999 / Final version: 2 June 1999 相似文献
100.
Page CP 《Journal of clinical pharmacology》1999,39(3):237-240
The use of theophylline for the treatment of asthma for 45 of the past 50 years has been for its ability to dilate bronchi. The problem has been that at the most effective bronchodilating dose, toxicity was too close for comfort. In the past 5 years, there has been resurgence in theophylline use at lower doses because of some well-documented anti-inflammatory and steroid sparing effects. 相似文献