THE ROLE OF SERUM FACTORS IN THE LYMPHOCYTE TRANSFORMATION TEST OF CHILDREN WITH ACUTE LEUKAEMIA

ABSTRACT. Révész, T., Szigeti, R. and Schuler, D. (Second Department of Paediatrics, Semmelweis University Medical School, Budapest, Hungary). The role of serum factors in the lymphocyte transformation test of children with acute leukaemia. Acta Paediatr Scand, 63: 715, 1974.—PHA-induced lymphocyte transformation tests were carried out in 14 children who were in full remission of acute lymphoblastic leukaemia and in 12 controls. Stimulation was assessed on the basis of tritiated thymidine uptake. For an adequate testing of lymphocyte reactivity, dose-response curves were established for each child. No significant difference could be observed in either the maximum response, or the shape of the curve, between the leukaemic and the control groups. Supplementation of the culture medium with autologous plasma instead of AB serum did not result in any significant inhibition or stimulation of lymphocyte reactivity to PHA. The lymphocyte response of both leukaemic and control children, however, was greatly reduced when sera from untreated leukaemic children was used. Allogeneic sera drawn from patients in remission exerted little or no inhibitory effect, while that obtained in relapse was again more inhibitory. The observed effect was not due to cytotoxic antibodies, nor due to natural iso-antibodies, but is thought to be a phenomenon closely connected with the active phase of malignant diseases. The exact nature of the agent is still far from being clarified at present.     

差示分光光度法测定牛黄类中成药中胆红素的含量

本文利用胆红素溶液在光照下易氧化变色及其吸收光谱发生显著变化的性质,在波长453 nm处,测其光照前后的吸收度差值(△A),△A与胆红素溶液浓度呈线性关系,对含牛黄的中成药(六应丸,六神丸和牛黄消炎丸)中胆红素进行了定量,并对其它组分进行了光照试验,排除了光照对其它组分的影响,从而确定了此法的可行性。     

压电频移中和萃取法测定微克量枸橼酸维静宁

用涂圈压电石英晶体频移一中和萃取法测定枸橼酸维静宁(咳必清),检出限0.02μg,在0.05～1.6μg范围内呈线性,相关系数0.999,变异系数为2.3%。与经典的中和萃取法相比,本法具有明显的优越性。     

依达拉奉对深低温保存大鼠断肢再植后缺血再灌注损伤的影响

目的:观察依达拉奉对深低温保存大鼠断肢再植后出现缺血再灌注损伤时的保护作用。方法:实验于2006-04/11在山东省立医院手足外科低温医学实验室完成。①选取健康成年Wistar大鼠36只,随机分为对照组、冷冻组和依达拉奉组3组,每组12只。②对照组只显露股动静脉而不结扎,其他2组大鼠截断右后肢,对断肢进行深低温冷冻保存处理(自结扎股动静脉至深低温保存约2h)。③1个月后,将冷冻保存的断肢复温、灌洗液洗脱,依达拉奉组所用灌洗液中含依达拉奉0.5mg/kg,行自体肢体回植(自液氮中取出肢体至恢复血供约2h),恢复肢体血供4h后取材,骨骼肌丙二醛含量,超氧化物歧化酶活性以及线粒体ATP酶活性,测定胫前肌含水量,光镜观察各组骨骼肌肌组织的结构改变。结果:36只全部进入结果分析。①骨骼肌丙二醛含量:依达拉奉组低于冷冻组[(10.37±1.25),(15.36±1.28)μmol/g,P<0.01]。②骨骼肌ATP酶和超氧化物歧化酶活性:依达拉奉组高于冷冻组[(206.2±45.2),(72.7±32.5)μkat/g;(83.9±5.2),(70.5±8.0)mkat/g;P均<0.01]。③胫前肌湿/干质量比值:依达拉奉组低于冷冻组(4.89±0.82,6.38±0.63,P<0.01)。④光镜结果显示依达拉奉组骨骼肌损伤程度明显轻于冷冻组。结论:依达拉奉可以降低肢体再灌注后肌肉组织中的氧自由基水平,减轻缺血再灌注对骨骼肌造成的损伤,对缺血再灌注骨骼肌具有保护作用。     

Regional odontodysplasia: Report of three cases

Regional odontodysplasia is a developmental anomaly of dental tissues with characteristic clinical, radiographic, and histologic appearances. It most commonly affects the maxillary anterior teeth of both the primary and permanent dentition, and occurs in females twice as often as in males. The pathogenesis is unknown. The clinical and histopathologic findings of regional odontodysplasia in three patients are discussed.