The Role of Catchment Vegetation in Reducing Atmospheric Inputs of Pollutant Aerosols in Ganga River

The role of woody perennials in the Ganga river basin in modifying the run-off quality as influenced by atmospheric deposition of pollutant aerosols was investigated. The concentration of seven nutrients and eight metals were measured in atmospheric deposits as well as in run-off water under the influence of five woody perennials. Nutrient retention was recorded maximum for Bougainvillea spectabilis ranged from 4.30 % to 33.70 %. Metal retention was recorded highest for Ficus benghalensis ranged from 5.15 % to 36.98 %. Although some species showed nutrient enrichment, all the species considered in the study invariably contribute to reduce nutrients and metal concentration in run-off water. Reduction in run off was recorded maximum for B. spectabilis (nutrient 6.48 %–40.66 %; metal 7.86 %–22.85 %) and minimum for Ficus religiosa (nutrient 1.68 %–27.19 %; metal 6.55 %–31.55 %). The study forms the first report on the use of woody perennials in reducing input of atmospheric pollutants to Ganga river and has relevance in formulating strategies for river basin management.     

Dietary intake of pollutant aerosols via vegetables influenced by atmospheric deposition and wastewater irrigation

Pot culture experiments were conducted to study dietary intake of heavy metals via vegetables, spinach (Spinacia oleracea L.), radish (Raphanus sativus L.) and tomato (Lycopersicon esculentum Mill) grown under the influence of atmospheric deposition and wastewater irrigation. The results indicated substantial accumulation of heavy metals in vegetables, which contribute significantly to dietary intake of total heavy metals ranging from 1.34 to 110.40 μg g?1 through leaves (spinach), 1.04 to 105.86 μg g?1 through root (radish) and 0.608 to 82.19 μg g?1 through fruits (tomato). Concentration of Cd, Ni and Pb in vegetables exceeded the safe limits of Prevention of Food Adulteration Act 1954. Health risk index for Cd and Pb exceeded the safe limits set by the United States Environmental Protection Agency. The study indicated that the atmospheric depositions as well as wastewater irrigation have significantly elevated the levels of heavy metals in dietary vegetables presenting a significant threat for the health of users.     

Appropriate and inappropriate imaging rates for prostate cancer go hand in hand by region, as if set by thermostat

Policy makers interested in containing health care costs are targeting regional variation in utilization, including the use of advanced imaging. However, bluntly decreasing utilization among the highest-utilization regions may have negative consequences. In a cross-sectional study of prostate cancer patients from 2004 to 2005, we found that regions with lower rates of inappropriate imaging also had lower rates of appropriate imaging. Similarly, regions with higher overall imaging rates tended to have not only higher rates of inappropriate imaging, but also higher rates of appropriate imaging. In fact, men with high-risk prostate cancer were more likely to receive appropriate imaging if they resided in areas with higher rates of inappropriate imaging. This "thermostat model" of regional health care utilization suggests that poorly designed policies aimed at reducing inappropriate imaging could limit access to appropriate imaging for high-risk patients. Health care organizations need clearly defined quality metrics and supportive systems to encourage appropriate treatment for patients and to ensure that cost containment does not occur at the expense of quality.     

Comparing three pedagogical approaches to psychomotor skills acquisition

BackgroundWe compared traditional pedagogical approaches such as time- and repetition-based methods with proficiency-based training.MethodsLaparoscopic novices were assigned randomly to 1 of 3 training conditions. In experiment 1, participants in the time condition practiced for 60 minutes, participants in the repetition condition performed 5 practice trials, and participants in the proficiency condition trained until reaching a predetermined proficiency goal. In experiment 2, practice time and number of trials were equated across conditions.ResultsIn experiment 1, participants in the proficiency-based training conditions outperformed participants in the other 2 conditions (P < .014); however, these participants trained longer (P < .001) and performed more repetitions (P < .001). In experiment 2, despite training for similar amounts of time and number of repetitions, participants in the proficiency condition outperformed their counterparts (P < .038). In both experiments, the standard deviations for the proficiency condition were smaller than the other conditions.ConclusionsProficiency-based training results in trainees who perform uniformly and at a higher level than traditional training methodologies.     

Rh phenotype,allele and haplotype frequencies among 51,857 blood donors in North India

Background

This study was performed to provide information on the frequencies of Rh antigens, alleles, phenotypes, and haplotypes from our region in India and to compare them with those from other races.

Materials and methods

This observational study was conducted on blood donors from March 2009 to August 2011 using a fully automated system for Rh typing of blood cells. The data were collected and calculations done to determine the antigen, phenotypes, allele and haplotype frequencies. The chi square test was used for comparisons between the results of our study and those of other studies.

Results

A total of 51,857 donors were included in this study. The most common Rh antigen found was “e”. DCCee was the most prevalent phenotype in our study with the phenotype distribution being significantly different between our study and other studies from different regions of the world.

Discussion

We have determined the prevalence of Rh antigens and Rh phenotypes in the North Indian blood donor population and derived the allele and haplotype frequencies in the same population. The Rh blood group distribution in this population was different from that in other populations.     

Common missense variant in the glucokinase regulatory protein gene is associated with increased plasma triglyceride and C-reactive protein but lower fasting glucose concentrations

OBJECTIVE—Using the genome-wide association approach, we recently identified the glucokinase regulatory protein gene (GCKR, rs780094) region as a novel quantitative trait locus for plasma triglyceride concentration in Europeans. Here, we sought to study the association of GCKR variants with metabolic phenotypes, including measures of glucose homeostasis, to evaluate the GCKR locus in samples of non-European ancestry and to fine- map across the associated genomic interval.RESEARCH DESIGN AND METHODS—We performed association studies in 12 independent cohorts comprising >45,000 individuals representing several ancestral groups (whites from Northern and Southern Europe, whites from the U.S., African Americans from the U.S., Hispanics of Caribbean origin, and Chinese, Malays, and Asian Indians from Singapore). We conducted genetic fine-mapping across the ∼417-kb region of linkage disequilibrium spanning GCKR and 16 other genes on chromosome 2p23 by imputing untyped HapMap single nucleotide polymorphisms (SNPs) and genotyping 104 SNPs across the associated genomic interval.RESULTS—We provide comprehensive evidence that GCKR rs780094 is associated with opposite effects on fasting plasma triglyceride (P_meta = 3 × 10⁻⁵⁶) and glucose (P_meta = 1 × 10⁻¹³) concentrations. In addition, we confirmed recent reports that the same SNP is associated with C-reactive protein (CRP) level (P = 5 × 10⁻⁵). Both fine-mapping approaches revealed a common missense GCKR variant (rs1260326, Pro446Leu, 34% frequency, r² = 0.93 with rs780094) as the strongest association signal in the region.CONCLUSIONS—These findings point to a molecular mechanism in humans by which higher triglycerides and CRP can be coupled with lower plasma glucose concentrations and position GCKR in central pathways regulating both hepatic triglyceride and glucose metabolism.Recently, in the genome-wide association Diabetes Genetics Initiative (DGI) Study for 19 traits, including plasma lipids, we provided evidence that the glucokinase (GCK) regulatory protein gene (GCKR) region was a novel quantitative trait locus associated with plasma triglyceride concentration (1). Of all single nucleotide polymorphisms (SNPs) tested, an intronic SNP at GCKR (rs780094) explained the greatest proportion of interindividual variability in plasma triglycerides (1).GCKR regulates GCK, which functions as a glucose sensor responsible for glucose phosphorylation in the first step of glycolysis. The discoveries that inactivating mutations in GCK cause maturity onset diabetes of the young type 2 (2) and activating GCK mutations lead to permanent hyperinsulinemic hypoglycemia (3) emphasize that GCK plays a major role in glucose metabolism. GCKR-deficient mice have reduced GCK expression but maintain nearly normal GCK activity and show impaired glucose clearance (4). Furthermore, adenoviral-mediated overexpression of GCKR in mouse liver increased GCK activity and lowered fasting blood glucose (5) and overexpression of GCK in liver led to lowered blood glucose and increased triglyceride concentrations (6,7). Thus, experimental evidence suggests that perturbation of the GCKR pathway has opposing effects of triglyceride and glucose metabolism.In our original report, SNP rs780094 in GCKR was associated with fasting triglyceride levels in two independent samples, each of Northern European ancestry (P = 3.7 × 10⁻⁸ and 8.7 × 10⁻⁸, respectively) (1). After initial identification and replication of a chromosomal region associated with a trait, key next steps include extension of the association finding to related phenotypes, validation of the association finding in different ethnicities, and fine- mapping to identify the putative causal variant. Recently, our initial finding was replicated in a Danish study in which a strong association was found between the rs780094 T allele and elevated fasting triglyceride levels but also lower insulin levels, better insulin sensitivity, and a moderately decreased risk of type 2 diabetes (8). In addition, recent genome-wide association studies identified an association between the same GCKR intronic SNP and C-reactive protein (CRP) levels (9,10).Hereby, we sought to examine the effect of SNP rs780094 on triglycerides and related metabolic traits, including fasting glucose concentrations, in 12 samples representing a range of ancestral groups and including a large prospective study with a mean follow-up time of 23 years. In addition, we performed fine-mapping in one of these samples to identify the strongest association signal in the region.     

Perinatal network consensus guidelines on the resuscitation of extremely preterm infants born at <27?weeks’ gestation

In spite of recent advances in perinatal care and an increase in survival of extremely preterm infants over the last few years, there remains a lack of consensus about practical aspects of resuscitation of extremely preterm infants born before 27?weeks' gestation. With this in the background, the working group of one of the Perinatal Networks in London, UK, set out to conduct a survey to explore the opinions of the doctors and nurses on resuscitation practices of infants born before 27?weeks' gestation, with the aim of developing consensus guidelines. The working group emailed a questionnaire to all neonatal units within the Perinatal Network to seek the views of paediatric medical and nursing staff on resuscitation of infants born at <27?weeks' gestation. The questionnaire was returned anonymously by post. The responses highlighted the difference of opinion that currently exists amongst the clinicians and nurses across the world around the resuscitation practices of extremely preterm infants; yet at the same time, there seemed to be some consensus on certain issues. Based on the survey (questionnaire) results and already existing literature, the working group of the North West London Perinatal Network (NWLPN) produced and implemented specific consensus guidelines on practical aspects of resuscitation for infants born before 27?weeks' gestation for the network. The network plans to audit these guidelines in future and also produce a parent information leaflet explaining the relevance of these guidelines.