1354 freshmen's dental investigation]

1354 freshmen have been investigated to discover the relationship between dental diseases and environments,living habits and personal sanitary conditions,because urban young men like sweet foods and snacks,the incidence of caries is greater in the cities than in the country,more people with tetracycline discolored tooth live in urban areas than in the rustic ones,which is due to dense living conditions, vulnerable to respiratory organs disease,medical conditions and convenience of taking tetracycline when ill;on the other hand,gingivitis is more common within the country students than within the city students,for those students from the country are used to brush their tooth after adolescence and usually have more dental calculus which is harmful to the gingiva.More than half of the investigated freshmen are ignorant of correct method of brushing and oral health and that is important factors of high rate of dental disease,according to the above reasons,the effective measures should educated to prevent dental disease.     

Latency of the jaw-opening reflex in the premature, preweaning and adolescent pig (hanford strain miniature pig, Sus scrofa)

The jaw-opening (digastric) reflex was elicited by electrical stimulation of oral mucosa in miniature pigs (Sus scrofa) varying in age from 5 days premature to 101 days post-term. The latency of reflex electromyographic activity varied between 12–14 ms in the most immature animals and 9–11 ms in the oldest animals. The very long-latency digastric responses found in the immature young of nesting mammals were not seen in the relatively precocious young of this species.     

Lactate dehydrogenase isoenzymes in human radicular cysts

Lactate dehydrogenase (LDH) isoenzymes of 15 clinically dormant radicular cysts were examined. A fragment from each cyst was homogenized, centrifuged and the LDH isoenzymes in the supernatant were separated by cellulose acetate paper electrophoresis. The strongest bands of the cysts were LDH-5 and LDH-4.     

Activation of vanilloid receptor 1 (VR1) by eugenol

The structural similarity of eugenol with capsaicin suggests that these two agents may share molecular mechanisms to produce their effects. We investigated the effects of eugenol in comparison with those of capsaicin using whole-cell patch clamp and Fura-2-based calcium-imaging techniques in a heterologous expression system and with sensory neurons. In vanilloid receptor 1 (VR1)-expressing human embryonic kidney (HEK) 293 cells and trigeminal ganglion (TG) neurons, eugenol activated inward currents, whereas capsazepine, a competitive VR antagonist, and ruthenium red (RR), a functional VR antagonist, completely blocked eugenol-induced inward currents. Moreover, eugenol caused elevation of [Ca(2+)](i), and this was completely abolished by both capsazepine and ruthenium red in VR1-expressing HEK 293 cells and TG neurons. Our results provide strong evidence that eugenol produces its effects, at least in part, via VR1 expressed by the sensory nerve endings in the teeth.     

Compositional analysis of human acquired enamel pellicle by mass spectrometry

Relatively little is known about the formation of the acquired enamel pellicle other than that it involves the selective adsorption of specific proteins from oral fluids. Previous studies on the identification of pellicle components have relied largely on immunological or enzymatic detection and have been hampered by the fact that only minute quantities of pellicle can be removed from tooth surfaces. The present work describes an improved method of harvesting pellicle that combines mechanical and chemical removal; this approach was used to investigate systematically the desorption of in vitro pellicle components with different solutions. Eleven major in vitro pellicle proteins were identified by using a combination of electrophoretic separation and matrix-assisted laser desorption/ionization-reflectron time-of-flight mass spectrometry. A similar analysis of in vivo-formed pellicle revealed the presence of intact statherin, lysozyme, albumin and amylase. Further analysis of in vivo pellicle by liquid chromatography-electrospray ionization mass spectrometry suggested the presence of numerous low molecular-weight fragments of precursor proteins. The protein composition of in vitro whole-salivary pellicle adsorbed to hydroxyapatite and that of in vivo enamel pellicle differed for proline, the result of a reduction in the content of acidic proline-rich proteins in the in vivo samples. Unique features of the oral environment such as enzymatic activities or mineral surface properties may account for these differences between in vivo and in vitro pellicle formation.     

Bone regeneration around an osseointegrated implant. A simultaneous approach in a fenestrated defect: a case report.

The use of a barrier membrane, with or without osseous allograft, has been shown to establish regeneration of osseous tissue around dental implants. Following three episodes of persistent symptomatic failed apicoectomy and subsequent tooth extraction, an osseointegrated implant was placed in a wide fenestrated defect. Demineralized freeze-dried bone allograft was covered by an occlusive expanded polytetrafluorethylene membrane. The reentry procedure revealed complete bone fill that followed the texture of the augmentation material beyond the previous buccal bony envelope.     

免疫化疗与TIUL得率及其杀瘤活性测定

Tumor-infiltrating lymphocyte (TIL) in the primary site of oral SCC treated with S-PVP of PVP therapy was collected respectively and its yield,that is calculating the amount of TIL per tumor tissue.The cytotoxicity of TIL against the target,Tca8113 was detected.The results showed that the yield of TIL in S-PVP group was three times over that in PVP group.At the ratio of 5 to 1 (effector to target) the cytotoxicity was 38% in S-PVP group and 32% in PVP group.There was no significant difference in its cytotoxicity between two groups.The results revealed that immunochemotherapy of S-PVP regimen for treating oral SCC had neither direct damage to tumor cell from the agents nor antitumor immune reactivity with BRM stimulating an increasing in numbers of TIL.     

MRI在口腔颌面部肿块诊断中的应用

作者对38例发生在口腔颌面部肿块患者采用MRI检诊。结合缅针穿吸缅胞学检查，并与CT扫描对比研究。结果提示：对口腔颌面部肿块。管状瘤或临床怀疑肿瘤有颅底、眶尖、翼腭窝、咽侧、颈部大血管神经累及者。MRI扫描成像清晰。软组织分辨率高。能早期发现骨质侵蚀。作者还对MRI在监测颌面部癌瘤切除术后局部是否复发进行初步观察。     

Conservative periodontal treatment combined with orthodontics for "hopeless" anterior teeth. A case report

This article describes the treatment of a patient with periodontally "hopeless" teeth by conservative periodontal therapy combined with subgingival chlorhexidine irrigation and orthodontic tooth movement. The 3-year follow-up post-treatment shows the relative success of the treatment, without the use of surgical modality or the extraction of any teeth.