全文获取类型
收费全文 | 2876篇 |
免费 | 167篇 |
国内免费 | 68篇 |
专业分类
耳鼻咽喉 | 26篇 |
儿科学 | 72篇 |
妇产科学 | 105篇 |
基础医学 | 283篇 |
口腔科学 | 51篇 |
临床医学 | 334篇 |
内科学 | 560篇 |
皮肤病学 | 46篇 |
神经病学 | 186篇 |
特种医学 | 246篇 |
外科学 | 442篇 |
综合类 | 60篇 |
一般理论 | 7篇 |
预防医学 | 262篇 |
眼科学 | 48篇 |
药学 | 227篇 |
肿瘤学 | 156篇 |
出版年
2022年 | 19篇 |
2021年 | 61篇 |
2020年 | 33篇 |
2019年 | 44篇 |
2018年 | 61篇 |
2017年 | 37篇 |
2016年 | 44篇 |
2015年 | 46篇 |
2014年 | 70篇 |
2013年 | 116篇 |
2012年 | 128篇 |
2011年 | 102篇 |
2010年 | 91篇 |
2009年 | 80篇 |
2008年 | 124篇 |
2007年 | 188篇 |
2006年 | 134篇 |
2005年 | 126篇 |
2004年 | 111篇 |
2003年 | 118篇 |
2002年 | 112篇 |
2001年 | 92篇 |
2000年 | 86篇 |
1999年 | 75篇 |
1998年 | 45篇 |
1997年 | 50篇 |
1996年 | 42篇 |
1995年 | 36篇 |
1994年 | 36篇 |
1993年 | 29篇 |
1992年 | 43篇 |
1991年 | 43篇 |
1990年 | 41篇 |
1989年 | 40篇 |
1988年 | 53篇 |
1987年 | 50篇 |
1986年 | 31篇 |
1985年 | 43篇 |
1984年 | 25篇 |
1983年 | 23篇 |
1982年 | 25篇 |
1981年 | 20篇 |
1980年 | 21篇 |
1979年 | 28篇 |
1978年 | 18篇 |
1977年 | 28篇 |
1976年 | 18篇 |
1973年 | 24篇 |
1972年 | 21篇 |
1970年 | 22篇 |
排序方式: 共有3111条查询结果,搜索用时 47 毫秒
21.
Humoral immune response to chlamydial genital infection of mice with the agent of mouse pneumonitis. 总被引:5,自引:11,他引:5
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The objective of this study was to characterize the humoral immune response to chlamydial genital infection of mice with the mouse pneumonitis agent (MoPn). With an enzyme-linked immunoabsorbent assay, immunoglobulin G antibodies to MoPn were first detected in plasma by day 14. Peak plasma antibody concentrations were reached by day 49, and this response did not decline significantly throughout the 300-day monitoring period. Immunoglobulin A against MoPn could first be detected in pooled vaginal washes by day 21 after infection and had reached peak concentrations by day 28, but anti-MoPn immunoglobulin G was not consistently present in secretions. The antibody response in secretions had declined slightly by day 300. Immunoblot analysis revealed that the early phase of the plasma antibody response to MoPn as a result of genital infection was against lipopolysaccharide, the major outer membrane protein, and a 62-kilodalton (kDa) protein. In secretions, early-phase immunoglobulin A antibodies were directed to the major outer membrane protein and lipopolysaccharide. Late reactions to 15-, 22-, and 83-kDa proteins in plasma were noted. Late reactions to the 62-kDa protein in secretions were also noted. The cause of these late responses remains unexplained. When mice were challenged intravaginally with MoPn at 50-day intervals after the primary infection, it was found that mice inoculated on day 100 or after were susceptible to reinfection. Susceptibility could not be related to a decline in the antibody concentration in plasma or secretions or in the antibody response to specific components of MoPn as measured by immunoblot analysis. 相似文献
22.
23.
Judith Ramsey Clare Austin Susan Wray 《Pflügers Archiv : European journal of physiology》1994,428(5-6):674-676
Changes in extracellular pH (pHo) induce changes in the intracellular pH (pHi) of cardiac myocytes that are slow and attenuated. Little however is known about the effects of changing pHo on the pHi of the coronary smooth muscle cells. We have therefore directly compared the effects of altering pHo on pHi of both coronary and cardiac myocytes. Carboxy-SNARF was used in single cells to measure pHi. Alteration of pHo caused corresponding changes in pHi that were large (70–80 % of pHo) and rapid in coronary myocytes compared to cardiac myocytes. In contrast, changes of pHi produced by weak acids or bases produced similar pHi responses in both types of cells. It is suggested that the differential effects of pHo on coronary and cardiac cells may be functionally significant, as it will allow rapid alteration of coronary perfusion to meet tissue needs, while maintaining cardiac output.Supported by the BHF and MRC 相似文献
24.
Inducible Nitric Oxide Synthase Does Not Affect Resolution of Murine Chlamydial Genital Tract Infections or Eradication of Chlamydiae in Primary Murine Cell Culture 总被引:1,自引:4,他引:1
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Kyle H. Ramsey Gurwattan S. Miranpuri Christoffer E. Poulsen Nancy B. Marthakis Laima M. Braune Gerald I. Byrne 《Infection and immunity》1998,66(2):835-838
Mice lacking inducible nitric oxide synthase (iNOS) or treated with iNOS inhibitors resolved chlamydial genital tract infections. Additionally, treatment of primary murine cell cultures with gamma interferon restricted chlamydial growth in the absence of nitric oxide. From these results, iNOS activity is unnecessary for the resolution of chlamydial genital tract infections in mice and inhibition of chlamydial growth in culture. 相似文献
25.
Kung FT; Chen WJ; Chou HH; Ko SF; Chang SY 《Human reproduction (Oxford, England)》1997,12(8):1649-1653
We report a rare case of early-stage endometrial adenocarcinoma in a 22
year old nullipara with polycystic ovaries undergoing conservative
treatment. Pretreatment evaluation including tumour grade, depth of
myometrial invasion, tumour size, hormone receptor status and flow
cytometric analysis indicated a favourable prognosis. The patient underwent
repeat endometrial curettage and a 6 month period of therapy with megestrol
acetate and tamoxifen. A combination contraceptive pill was then prescribed
to ensure withdrawal of the menstrual cycle thereafter. Now, 1 year after
the last curettage, there is no evidence of disease. During the treatment
period, hysteroscopy allowed for a more precise approach in panoramically
examining the tumour nest in the endometrial cavity, and the subsequent
endometrial response to hormone therapy. Laparoscopy using bulldog clamps
applied to the isthmic portion of the Fallopian tubes prevented i.p. spread
of endometrial tissue from retrograde regurgitation during hysteroscopy.
Laparoscopic ovarian electrocautery resulted in the reduction of abnormal
hypervascularization on the surface of polycystic ovaries postoperatively
but caused a peri-ovarian adhesion complication. It is interesting that
this case posed a unique opportunity to demonstrate the tumour regression
under the assistance of laparoscopy and hysteroscopy.
相似文献
26.
An inexpensive assay for von Willebrand factor using microtitration plates, formalin-fixed platelets, and ristocetin is described. With this technic, the assay for von Willebrand factor is simple enough to be considered for use in any diagnostic laboratory for patients with prolonged bleeding times. 相似文献
27.
Lactate production in isolated perfused rat lung 总被引:1,自引:0,他引:1
28.
Lim DJ Rubenstein AE Evans DG Jacks T Seizinger BG Baser ME Beebe D Brackmann DE Chiocca EA Fehon RG Giovannini M Glazer R Gusella JF Gutmann DH Korf B Lieberman F Martuza R McClatchey AI Parry DM Pulst SM Ramesh V Ramsey WJ Ratner N Rutkowski JL Ruttledge M Weinstein DE 《Journal of neurogenetics》2000,14(2):63-106
29.
Richard Meehan Ulric Duncan Laureen Neale Gerald Taylor Harold Muchmore Nan Scott Keith Ramsey Eric Smith Paul Rock Randall Goldblum Charles Houston 《Journal of clinical immunology》1988,8(5):397-406
We investigated the effects on immune function after progressive hypobaric hypoxia simulating an ascent to 25,000 ft (7620 m) over 4 weeks. Multiple simultaneousin vitro andin vivo immunologic variables were obtained from subjects at sea level, 7500 ft (2286 m), and 25,000 ft during a decompression chamber exposure. Phytohemag-glutinin-stimulated thymidine uptake and protein synthesis in mononuclear cells were reduced at extreme altitudes. Mononuclear-cell subset analysis by flow cytometry disclosed an increase in monocytes without changes in B cells or T-cell subsets. Plasma IgM and IgA but not IgG levels were increased at altitudes, whereas pokeweed mitogen-stimulatedin vitro IgG, IgA, and IgM secretion was unchanged. During exposure to 25,000 ft,in vitro phytohemagglutinin-stimulated interferon production and natural killer-cell cytotoxicity did not change statistically, but larger intersubject differences occurred. IgA and lysozyme levels (nasal wash) and serum antibodies to nuclear antigens were not influenced by altitude exposure. These results suggest that T-cell activation is blunted during exposure to severe hypoxemia, whereas B-cell function and mucosal immunity are not. Although the mechanism of alteredin vitro immune responsiveness after exposure to various environmental stressors has not been elucidated in humans, hypoxia may induce alterations in immune regulation as suggested byin vitro immune assays of effector-cell function.Some of this study's results were presented as an abstract at the FASEB meeting in St. Louis, Missouri, 1986. 相似文献
30.
Identification of the multiple endocrine neoplasia type 1 (MEN1) gene. The European Consortium on MEN1 总被引:10,自引:0,他引:10
Lemmens I; Van de Ven WJ; Kas K; Zhang CX; Giraud S; Wautot V; Buisson N; De Witte K; Salandre J; Lenoir G; Pugeat M; Calender A; Parente F; Quincey D; Gaudray P; De Wit MJ; Lips CJ; Hoppener JW; Khodaei S; Grant AL; Weber G; Kytola S; Teh BT; Farnebo F; Thakker RV 《Human molecular genetics》1997,6(7):1177-1183