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41.
Roudkenar MH Halabian R Oodi A Roushandeh AM Yaghmai P Najar MR Amirizadeh N Shokrgozar MA 《Archives of medical research》2008,39(4):402-407
BACKGROUND: One of the major consequences in beta- thalassemia is iron overload. Oxidative statuses have been reported in beta-thalassemia patients by several studies. It has been proven that iron plays a critical role in the formation of reactive oxygen species (ROS). More recently, we have found the induction of Lcn2/NGAL expression under oxidative stress condition. In this study, it was assumed that NGAL should be upregulated in beta-thalassemia patients because of oxidative stress condition. METHODS: Assessment of NGAL expressions in 25 adult beta-thalassemia and 9 pediatric patients was performed by semiquantitative RT-PCR, real-time RT-PCR and ELISA. RESULTS: Adult beta-thalassemia patients upregulated NGAL expression compared with the normal samples but no upregulation was observed in pediatric patients. CONCLUSIONS: Upregulation may play an important role in decreasing ROS or iron in beta-thalassemia patients. 相似文献
42.
Giandomenico Roviello Laura Zanotti Maria Rosa Cappelletti Angela Gobbi Martina Dester Giovanni Paganini Chiara Pacifico Daniele Generali Raheleh Roudi 《Clinical and experimental medicine》2018,18(1):15-20
EGFR tyrosine kinase inhibitors (TKIs) such as erlotinib, gefitinib, and afatinib changed dramatically the history of metastatic non-small cell lung cancer (NSCLC) harbouring EGFR mutations. However, not enough data are available on the efficacy of these targeted drugs in elderly patients. The aim of this study is to analyse the available clinical data evaluating the efficacy of anti-EGFR therapies in elderly patients with advanced NSCLC carrying EGFR mutations. A literature-based meta-analysis of the results of randomized clinical trials was undertaken. Relevant publications from PubMed, the Cochrane Library, and abstracts from American Society of Clinical Oncology meetings were searched. Progression-free survival (PFS), as a measure of the efficacy of treatment, was the primary outcome investigated. The pooled analysis revealed an overall significant improvement in PFS (HR = 0.44, 95% CI 0.28–0.69; p = 0.0004) with the use of EGFR TKIs in EGFR-mutated NSCLC. The data stratification per age subgroups showed that EGFR TKIs were more effective in prolonging PFS in elderly patients, with HR 0.39 (p = 0.008), in comparison with young patients (HR = 0.48; p = 0.04). The results of this study suggest that EGFR TKIs have a significant effect in slowing down diseases progression in elderly patients with advanced NSCLC, therefore representing a valid therapeutic option in this age group. 相似文献
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Ezzatollah Fathi Seyed Mahdi Nassiri Nahid Atyabi Seyed Hossein Ahmadi Mohammad Imani Raheleh Farahzadi Shahram Rabbani Shahram Akhlaghpour Mohammad Sahebjam Mohammad Taheri 《Journal of tissue engineering and regenerative medicine》2013,7(9):697-707
Hydrogels are currently used as interesting constructs for the delivery of proteins. In this study, a novel polyvinyl alcohol–dextran (PVA–Dex) blend hydrogel was used for controlled delivery of basic‐fibroblast growth factor (bFGF). These biocompatible constructs were sutured to the epicardium as patches on the heart surface to provide slow release of bFGF to the infarcted site in an ovine model of myocardial infarction (MI). Eighteen sheep were randomly divided into three groups (n = 6 each), including group I (control without any patch and bFGF), group II (patch without bFGF) and group III (patch incorporating 100 µg bFGF). They were subjected to coronary artery ligation after lateral thoracotomy, and then in groups II and III the patches were implanted 20–30 min after MI. Cardiac function was assessed by both echocardiography and magnetic resonance imaging (MRI) 2 months after implantation. Then the animals were sacrificed and the hearts subjected to histopathological examination, immunohistochemistry and electron microscopy. Heart lysates were subject to protein expression analysis through western blotting. The results showed that sustained release of bFGF using PVA–Dex blend hydrogel strongly stimulated angiogenesis and increased wall thickness index in the infarcted myocardium. The patch also significantly attenuated the increase in left ventricular end‐systolic diameter, but it did not improve cardiac function within 2 months of myocardial infarction. In conclusion, PVA–Dex gel incorporating bFGF can be used as a sustained release construct for therapeutic angiogenesis in ischaemic heart disease. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
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Mahmood Jeddi‐Tehrani Raheleh Torabi Afsaneh Mohammadzadeh Soheila Arefi Mohammad Keramatipour Hojjat Zeraati Amir Hasan Zarnani Mohammad Mehdi Akhondi Jafar Mahmoudian Ahmad Reza Mahmoudi Saeed Zarei 《American journal of reproductive immunology (New York, N.Y. : 1989)》2010,64(3):212-217
Citation Jeddi‐Tehrani M, Torabi R, Mohammadzadeh A, Arefi S, Keramatipour M, Zeraati H, Zarnani AH, Akhondi MM, Mahmoudian J, Mahmoudi AR, Zarei S. Investigating association of three polymorphisms of coagulation factor XIII and recurrent pregnancy loss. Am J Reprod Immunol 2010; 64: 212–217 Problem Among important suspected causes of thrombophilia in women with recurrent pregnancy loss (RPL) are the polymorphisms of coagulation factor XIII (FXIII) gene. We performed a case–control study on the association between three polymorphisms of factor XIII (FXIII G103T, FXIII A614T and FXIII C1694T) and RPL in Iranian women. Method of study DNA samples from peripheral blood of 100 female patients with at least two recurrent abortions, as case group, and 100 healthy women with history of at least two successful deliveries were subjected to PCR‐RFLP, and the frequencies of the polymorphisms were calculated and compared between the two groups. Results The prevalence of FXIII G103T polymorphism was 29% in the case group and 17% in the control group (P = 0.158). The frequencies of FXIII A614T and FXIII C1694T were 84% and 66% in the case group and 48% and 31% in the control group (P < 0.001 and P < 0.001), respectively. The two latter polymorphisms are associated with RPL in Iranian women and increase the risk of RPL. A correlation was also found between FXIII A614T and FXIII C1694T polymorphisms (P < 0.001). Conclusion We suggest the evaluation of FXIII A614T and FXIII C1694T polymorphisms in women with RPL. 相似文献
47.
Zahra Orouji Amir Bajouri Mahshid Ghasemi Parvaneh Mohammadi Nasrin Fallah Atefeh Shahbazi Mohammad Rezvani Fatemeh Vaezirad Zahra Khalajasadi Ahad Alizadeh Ehsan Taghiabadi Sara Dashtbozorgi Raheleh Aghdami Seyedeh Esmat Hosseini Hossein Bahahrvand Saeed Shafieyan Nasser Aghdami 《Journal of dermatological science》2018,89(1):52-59
Background
Recently, we introduced intralesional injection of autologous epidermal cells as a safe and feasible approach for transplantation in patients with stable vitiligo. This approach resulted in less pain during and after the procedure, no scarring or cobblestone formation at the recipient site, and was more feasible to perform on curved surfaces such as joints, lips, eyelids, ears, and face.Objective
In this study, we aimed to investigate the long-term efficacy and safety of this transplantation technique.Methods
In this open-label and single-arm clinical trial, we enrolled 300 patients with stable vitiligo. We obtained a partial thickness normo-pigmented skin specimen from the patients’ thigh-buttock junction with an area of one tenth to one third of the recipient site area. The epidermal cell suspension was prepared by processing the autologous skin specimen. We injected the cell suspension into 1060 vitiligo patches in 300 patients. Patients did not use any adjuvant phototherapy during the study. An experienced dermatologist and patients respectively defined the repigmentation score and self-assessment score at regular follow-up visits for up to 30 months after treatment. The scores represented the repigmentation percentage as follows: 0 (0), I (1%–24%), II (25%–49%), III (50%–74%), and IV (75%–100%).Results
The mean repigmentation score at 3 months post-transplantation was 1.12 ± 0.73. A significant upward trend existed in the mean repigmentation score until 9 months after cell transplantation, when the mean repigmentation score reached to 1.98 ± 1.20. At 9 months after treatment, repigmentation of >50% was obtained in 32.2% of treated patches. Acquired repigmentation remained stable in 79.3% of treated patches during the follow-up period. The number of received cells per cm2 positively influenced the repigmentation score. Patches located on face, neck and trunk showed significantly higher response to the treatment.Conclusion
The results of our study demonstrated efficacy and safety of autologus epidermal cell transplantation on repigmentation of vitiligo patches. The achieved repigmentation was stable in the majority of treated patches during the follow-up period. 相似文献48.
Di Chen Afsun Sahin Wendy R. Kam Yang Liu Raheleh Rahimi Darabad David A. Sullivan 《The ocular surface》2018,16(3):382-389
Purpose
Lipopolysaccharide (LPS), a bacterial endotoxin, is known to stimulate leuokotriene B4 (LTB4) secretion by human corneal (HCECs), conjunctival (HConjECs) and meibomian gland (HMGECs) epithelial cells. We hypothesize that this LTB4 effect represents an overall induction of proinflammatory gene expression in these cells. Our objective was to test this hypothesis.Methods
Immortalized HCECs, HConjECs and HMGECs were cultured in the presence or absence of LPS (15?μg/ml) and ligand binding protein (LBP; 150?ng/ml). Cells were then processed for RNA isolation and the analysis of gene expression by using Illumina BeadChips, background subtraction, cubic spline normalization and GeneSifter software.Results
Our findings show that LPS induces a striking increase in proinflammatory gene expression in HCECs and HConjECs. These cellular reactions are associated with a significant up-regulation of genes associated with inflammatory and immune responses (e.g. IL-1β, IL-8, and tumor necrosis factor), including those related to chemokine and Toll-like receptor signaling pathways, cytokine-cytokine receptor interactions, and chemotaxis. In contrast, with the exception of Toll-like signaling and associated innate immunity pathways, almost no proinflammatory ontologies were upregulated by LPS in HMGECs.Conclusions
Our results support our hypothesis that LPS stimulates proinflammatory gene expression in HCECs and HConjECs. However, our findings also show that LPS does not elicit such proinflammatory responses in HMGECs. 相似文献49.
The aim of this study was to evaluate the cytotoxicity and gelation of thermosensitive chitosan-beta-glycerol phosphate (GP) solutions, which undergo sol-gel transition around body temperature. Chitosan 0.5-2% (w/v) mixed with GP 5-20% (w/v) solutions all gel at 37 degrees C and possess pH around the physiological range. High GP and chitosan concentrations result in faster gelation time. Extracts of all chitosan concentrations mixed with or without 5% (w/v) GP and 2% (w/v) chitosan combined with 10% (w/v) GP demonstrated up to 34% increase in proliferation rate of goat bone marrow derived mesenchymal stem cells when compared with control medium. Extracts from all other chitosan-GP combinations resulted in reduced cell proliferation relative to control medium. Increasing GP content in the gel resulted in a linear increase in the osmolality of the extracts in contact with the gels. The results of this study indicate that chitosan-GP is a biocompatible hydrogel, extracts of which can stimulate mesenchymal stem cell proliferation at certain concentrations. This material is therefore a promising vehicle for cell encapsulation and injectable tissue-engineering applications. 相似文献
50.
Hojjat Rahmani Raheleh Maleki Mahboubeh Khaton Ghanbari Masoud Behzadifar 《Ethiopian journal of health sciences》2022,32(2):453
BackgroundPrimary healthcare (PHC) plays an important role in achieving universal health coverage (UHC). The SERVQUAL instrument is the tool for evaluating the quality of services in the health sector. The main purpose of this study is to evaluate the quality of services provided in PHC in Iran using the SERVQUAL instrument.Materials and MethodWe searched eight databases from January 2000 to September 2021. We analyzed the mean of various SERVQUAL instrument items using the DerSimonian-Laird approach via a random model with 95% confidence interval. Also, we used I2 to evaluate the heterogeneity of the studies.ResultsFinally, 17 studies were chosen for analysis in the present study. There were 8,767 study participants, out of which 8,237 were female and 530 were male. The mean dimensions of perception were as follows: Tangibles = 3.71, reliability = 4, responsiveness = 3.79, assurance = 3.83, and empathy = 3.86. For the expectation, the mean dimension were: Tangibles = 4.46, reliability = 4.46, responsiveness = 4.36, assurance = 4.36, and empathy = 4.36 respectively. The total gap quality between perception and expectation was -0.53.ConclusionAll dimensions of quality based on SERVQUAL were negative, and the quality of service in PHC is not satisfactory. Therefore, policymakers must adopt serious and effective programs to improve services in this area. We also recommend that quality management of services in PHC in Iran should move toward comprehensive optimization in all areas, and quality in this area should be a priority. 相似文献