中医药治疗恶性肿瘤的研究进展

阐述了中医对恶性肿瘤病因病机的认识及治则治法，对目前中医药治疗恶性肿瘤的几种新思路进行了探讨．在指出中医药治疗恶性肿瘤中存在的问题的同时，提出了笔者的几点建议。     

榛子叶化学成分研究(Π)

目的　对榛子叶中的鞣质成分进行了研究 ,开发利用黑龙江省榛子叶资源。方法　利用 1 H- NMR,1 3C- NMR,FAB- MS,CD谱及 1 H- 1 HCOSY二维谱与化学方法鉴定。结果　从中得到 4个化学成分 :榛叶素 B(heterophylliin B, ) ,tellimagrandin ( ) ,casuarictin ( ) ,casuarinin ( )。结论　榛叶素 B是一个新的鞣花二聚体成分     

c-raf-1反义寡核苷酸对人卵巢癌细胞放射敏感性的影响

目的　探讨癌基因 c- raf- 1的反义寡核苷酸对人卵巢癌细胞放射敏感性的影响。方法　设立 c- raf- 1正义寡核苷酸组和非处理组 (仅加入等量的脂质体 )作对照 ,用 RT- PCR检测卵巢癌细胞处理前后 c- raf- 1表达水平的变化。用平板克隆形成试验检测人卵巢癌细胞株 SKOV3在脂质体 - c- raf- 1反义寡核苷酸作用前后受 6 0 Coγ射线不同剂量照射后的集落形成率。结果　 c- raf- 1反义寡核苷酸能抑制 c- raf- 1癌基因的表达 ,经脂质体介导的 c- raf- 1反义寡核苷酸作用的卵巢癌细胞经γ射线照射后其集落形成率较转染正义寡核苷酸组及单纯照射组明显下降 (P<0 .0 1) ,而转染 c- raf- 1正义寡核苷酸组的集落形成率与单纯照射组相比无明显差异 (P>0 .0 5 )。结论　 c- raf- 1反义寡核苷酸通过抑制 c- raf- 1的表达降低人卵巢癌细胞株 SKOV 3放射抗拒性。该作用可能与 c- raf- 1反义寡核苷酸抑制了引起细胞辐射抗拒的细胞信号转导途径有关     

MIC-1 serum level and genotype: associations with progress and prognosis of colorectal carcinoma.

PURPOSE: Macrophage inhibitory cytokine-1 (MIC-1) is a divergent member of the tumor growth factor beta (TGF-beta) superfamily. Several observations suggest that it plays a role in colorectal carcinoma (CRC). In particular, MIC-1 is markedly up-regulated in colorectal cancers as well as in premalignant adenomas. This study examines the relationship of serum MIC-1 levels and genotypes to clinical and pathologic features of colonic neoplasia. Experimental Design: We confirmed the presence of MIC-1 in CRC tissue and the cell line CaCo-2. The normal range for serum MIC-1 levels was defined in 260 healthy blood donors, and the differences between normal subjects and 193 patients having adenomatous polyps or CRC were then determined. In a separate cohort of 224 patients, we evaluated the relationship of MIC-1 serum level and genotype to standard tumor parameters and outcome measures. RESULTS: MIC-1 was expressed in CRC tissue and the cancer cell line CaCo-2. There was a progressive increase in serum MIC-1 levels between normal individuals [mean (M) = 495 pg/ml, SD = 210), those with adenomatous polyps (M = 681 pg/ml, SD = 410), and those with CRC (M = 783 pg/ml, SD = 491)]. Serum MIC-1 level was correlated with the extent of disease so that the levels were higher in patients with higher Tumor-Node-Metastasis stage. There were significant differences in time to relapse and overall survival between subjects with different MIC-1 levels and genotypes. CONCLUSIONS: This study identifies a strong association between MIC-1 serum levels and neoplastic progression within the large bowel. We suggest that the measurement of serum MIC-1 levels and determination of MIC-1 genotype may have clinical use in the management of patients with CRC.     

石蜡包埋组织DNA检测在判断畸形儿病因中的应用

目的：对１９８５～１９９５年在我院尸体检查证实为畸形死亡的３９例围产儿石蜡包埋组织标本，进行有关病原体的检测，以明确围产儿致畸原因。方法：用改良的水浴脱蜡、直接裂解及酶解法，进行石蜡包埋的死亡围产儿肝、脑组织的弓形体、巨细胞病毒及Ⅱ型单纯疱疹病毒的聚合酶链反应（ＰＣＲ）检测。同时对送检胎盘者，取胎盘石蜡包埋组织，分别作上述病原体的检测。结果：３９例中２５例检出病原体，检出率为６４％。其中泌尿生殖系统畸形１３例中９例检出病原体，神经管缺陷１２例中８例检出病原体。此两种畸形中病原体的检出率高达６８％。结论：本组资料证实宫内感染是围产儿致畸的重要原因之一，并对胎盘感染常见的形态学变化及畸形儿胎盘常规送检的重要性进行了讨论。     

输卵管妊娠保留输卵管手术后综合疗法的宫内妊娠探讨

对输卵管妊娠52例有生育要求者，其中28例行切开输卵管壶腹部取胚胎术，10例行输卵管峡部破裂口修补术，7例行输卵管伞部成形术，5例行输卵管峡部端端吻合术，2例行输卵管子宫角部植入术。术后给MTX与中药等综合治疗2～5个月，对其中45例行输卵管通畅检查，双侧输卵管通畅4O例，通畅率为88.9％；有41例宫内妊娠，妊娠率为78.9％，另有1例再次异位妊娠。提示：对有生育要求的输卵管妊娠尽量根据输卵管的不同情况采用相应的手术方法，并应用有效的术后综合措施，对保留患者的生育功能和提高妊娠率有重要意义。     

GLOMUS TUMORS──A REVIEW OF 8 CASES WITH INTRODUCTION OF A MODIFIED COMBINED INTRA-AND EXTRACRANIAL APPROACH

Eight cases of glomus tumors hospitalized in our department from 1982 through 1995 were reviewed. It comprised of five glomus tympanic tumors and three glomus jugular tumors. Discussion was centered on it’s contemporary diagnosis, classification and surgical treatment with introduction of a modified combined intra-and extracranial surgical approach to resect the extensive glomus tumors.     

THE HUMORAL ANTITUMOR RESPONSES INDUCED BY IL-4 GENE-MODIFIED TUMOR VACCINE

ＴＨＥＨＵＭＯＲＡＬＡＮＴＩＴＵＭＯＲＲＥＳＰＯＮＳＥＳＩＮＤＵＣＥＤＢＹＩＬ４ＧＥＮＥＭＯＤＩＦＩＥＤＴＵＭＯＲＶＡＣＣＩＮＥ１ＹｕＹｉｚｈｉ于益芝ＣａｏＸｕｅｔａｏ２曹雪涛ＺｈａｎｇＭｉｎｇｈｕｉ张明徽ＬｅｉＨｏｎｇ雷虹ＴａｏＱｕｎ陶群Ｄｅｐ...