Assessment of Carbendazim Residues and Safety in Celery Under Different Cultivation Conditions

Although the carbendazim is widely used to manage spot blight in celery cultivation, information on residues identified is of interest. In this study, we examined the dissipation and residual amounts of carbendazim in celery and soil under different cultivation methods when using the suggested dose and ten times of that and the bioconcentration factor of carbendazim for celery. The results showed that when celery leaves were sprayed with the suggested dose, the half-lives in a celery field and greenhouse were 2.75 days and 3.29 days, respectively. When the soil matrix was sprayed with the recommended dose before cultivation, the half-lives of carbendazim residues were 16.86 days and 11.97 days. We also conducted a long-term dietary risk assessment using the corresponding criteria. The results showed that, in China, the use of carbendazim at a dose of 0.022 g/m² is safer and more reasonable when the harvest interval is 28 days.

     

4型腺病毒载体的构建和β－半乳糖苷酶基因的表达

以４型腺病毒疫苗株感染Ａ５４９细胞，提取病毒ＤＮＡ，将ＥｃｏＲＩ消化的Ｄ片段（７０．５－８３．０基因图谱单位）克隆入ｐＵＣ１８质粒，得ｐＡｄ４（７０．５－８３）质粒，质粒ｐＡｄ４（７０．５－８３）和ｐＡｄ４Ｃ１－２５经酶切、系列亚克隆、加接头等方法得到大部分和部分缺失Ｅ３区的重组质粒。聚合酶链反应（ＰＣＲ）法获得ｐｏｌｙ（Ａ），构建约８００ｂＰ腺病毒晚期表达盒，在所构建的腺病毒重组质粒Ｅ３缺失区或Ｅ４与ＩＴＲ间插入表达盒，得到可同时表达２个或２个以上外源基因和保留了Ｅ３区编码分子量为１９３００糖蛋白基因的３种Ａｄ４载体。将ｌａｃＺ基因插入载体表达区，与Ｂｅｌｌ消化的Ａｄ４ＤＮＡＡ片段共转染Ａ５４９细胞，ＯＮＰＧ法检测证实所构建的载体和表达盒功能良好。本项工作对于在国内研究口服活疫苗及开展基因治疗均具有重要意义。     

大学生自我表露及其与主观幸福感的关系

目的:探讨大学生自我表露、主观幸福感现况及二者的关系.方法:根据浙江大学164名学生的问卷调查结果对Jourard SM自我表露问卷进行修订,用修订的自我表露问卷和幸福感指数量表对浙江大学的356名学生进行问卷调查.结果:不同信息类别的自我表露评分差异显著(F=50.24,P＜0.001),其中品味和兴趣(1.85±0.41)、学习(工作)(1.87±0.43)的自我表露评分均高于态度和看法(1.70±0.32)(t=7.870、8.256)、金钱(1.60±0.41,t=10.517、11.429)、个性(1.70±0.39)、身体(1.71±0.42)(P均＜0.001).目标人的主效应显著(有男/女朋友者、无男/女朋友者的自我表露问卷评分差异均有显著性,F=21.14、13.88,P＜0.001),信息类别和目标人的交互作用显著(F=76.15,P＜0.001).在金钱、身体、学习或工作方面大学生向父母表露多于向男、女性朋友(如金钱上:母亲1.81±0.59、父亲1.73±0.58,男朋友1.42±0.44,女朋友1.40±0.44;F=116.84,P=0.000).女性的表露程度高于男性(1.79±0.31/1.71±0.32,t=2.03,P=0.044).自我表露与主观幸福感呈正相关(r=0.16,P=0.003).分层回归显示自我表露对预测主观幸福感作出了新的贡献(△R2=0.021,P=0.006).结论:信息类别、目标人、被试性别会影响自我表露的程度,提升表露水平有助于改善主观幸福感.     

Endogenous estrogen status, but not genistein supplementation, modulates 7,12-dimethylbenz[a]anthracene-induced mutation in the liver cII gene of transgenic big blue rats

A growing number of studies suggest that isoflavones found in soybeans have estrogenic activity and may safely alleviate the symptoms of menopause. One of these isoflavones, genistein, is commonly used by postmenopausal women as an alternative to hormone replacement therapy. Although sex hormones have been implicated as an important risk factor for the development of hepatocellular carcinoma, there are limited data on the potential effects of the estrogens, including phytoestrogens, on chemical mutagenesis in liver. Because of the association between mutation induction and the carcinogenesis process, we investigated whether endogenous estrogen and supplemental genistein affect 7,12-dimethylbenz[a]anthracene (DMBA)-induced mutagenesis in rat liver. Intact and ovariectomized female Big Blue rats were treated with 80 mg DMBA/kg body weight. Some of the rats also received a supplement of 1,000 ppm genistein. Sixteen weeks after the carcinogen treatment, the rats were sacrificed, their livers were removed, and mutant frequencies (MFs) and types of mutations were determined in the liver cII gene. DMBA significantly increased the MFs in liver for both the intact and ovariectomized rats. While there was no significant difference in MF between the ovariectomized and intact control animals, the mutation induction by DMBA in the ovariectomized groups was significantly higher than that in the intact groups. Dietary genistein did not alter these responses. Molecular analysis of the mutants showed that DMBA induced chemical-specific types of mutations in the liver cII gene. These results suggest that endogenous ovarian hormones have an inhibitory effect on liver mutagenesis by DMBA, whereas dietary genistein does not modulate spontaneous or DMBA-induced mutagenesis in either intact or ovariectomized rats.     

Homer 1b/c通过内质网功能调节由谷氨酸诱发的HT22细胞自噬

目的　研究Homer1b/c蛋白在谷氨酸诱发的细胞自噬中的作用及机制。方法　选用小鼠海马细胞系HT22细胞,通过500 ?mol/L谷氨酸处理建立细胞损伤模型。用siRNA慢病毒转染方式下调Homer1b/c表达和10 ?mol/LBAPTA-AM（1,2-双(2-氨基苯氧基)乙烷-N,N,N`,N`-四乙酸四乙酸甲酯,钙离子螯合剂）、10 mmol/L4-PBA（4-苯基丁酸,内质网应激抑制剂）分别抑制细胞内钙离子释放和内质网应激后,使用蛋白质印迹法检测Homer1b/c,自噬蛋白Beclin-1、微管相关蛋白轻链3（LC3）,以及内质网应激标志蛋白CHOP（人内质网应激相关蛋白）、GRP-78(葡萄糖调节蛋白78)的表达水平。每组实验均进行3次,采用独立样本t检验和单因素方差分析进行统计学分析。结果　谷氨酸处理HT22细胞12 h后,Beclin-1表达和LC3-Ⅱ/LC3-Ⅰ比值均升高（P<0.05）,下调Homer1b/c表达可降低Beclin-1表达和LC3-Ⅱ/LC3-Ⅰ比值的升高程度（P<0.05）。抑制细胞内钙离子释放和抑制内质网应激均能降低Beclin-1表达和LC3-Ⅱ/LC3-Ⅰ比值的升高程度（P<0.05）。然而在下调Homer1b/c表达后,抑制细胞内钙离子释放和抑制内质网应激未能进一步降低Beclin-1表达和LC3-Ⅱ/LC3-Ⅰ比值的升高程度（P<0.05）。结论　Homer1b/c能够调节谷氨酸诱导的自噬,其调节作用可能与内质网功能有关。     

Preliminary study on immunotoxin for the prevention of Schistosomiasis japonica

To investigate the effects of immunotoxin (bio-missile) on the prevention of schistosomiasis. The monoclonal antibody NP11-4, located in out-layer membrane of adult worm, membrane of cercariae, membrane of schistosomula, shell of egg and membrane of miracidium in egg, was combined with artesunate (produced by Guilin Pharmaceutical Factory of China) by chemical method to fabricate immunotoxin.     

普罗布考对体外培养的主动脉平滑肌细胞NF—kB活性的影响

目的：观察普罗布考（probucol)对血管平滑肌细胞（VSMCs)核因子kB(NF-kB)活性的调控作用,以探讨probucol在动脉粥样硬化（AS）和经皮腔内冠状动脉成形术后再狭窄（RS）防治中的某些可能的作用机制。方法：采用电泳迁移位移测定法观察probucol对VSMCsNF-kB活性的影响。结果：H2O2或胎牛血清（NCS）处理VSMCs72h后,NF-kB活性均明显增强;而加入100umol/Lprobucol后,NF-kB活性受到部分抑制,抑制率分别为37.1%和14.8%。结论：Probucol抑制NCS、H2O2诱导的VSMCsNF-kB的活性,可能是其临床有效防治RS及AS的作用机制之一。     

阴道炎患者HPV6/11-DNA定量分析

目的：了解女性阴道炎患者人乳头瘤病毒6／11型（HPV6／11）的感染情况。方法：荧光定量聚合酶链反应（FQ－PCR）技术检测258例门诊患者阴道分泌物中HPV6／11的病毒拷贝数。结果：共检出HPV6／11阳性者94例,阳性率36．43％,拷贝数在10^5以上者78例,占阳性者中82．98％,40岁以上年龄组阳性率高且病毒复制量均在10^5以上。结论：（1）中老年阴道炎患者就诊晚,感染重。（2）FQ－PCR检测HPV敏感,快速,准确,特别是其定量特点对临床很有意义。