偏振光游标式调节检测仪的研制

介绍一种根据Scheiner氏盘原理研制的偏振光游标式调节检测仪 ,专用于人眼张力性调节、调节反应和远、近点的测量。通过临床检测 ,该仪器参数稳定 ,重复性好 ,反应灵敏 ,为与眼动参数有关的近视眼研究领域提供了可靠的实验方法和设备。     

The extent and pattern of infiltration along the bronchial wall in central type lung cancer

Gross and microscopic study was made in fifty specimens emphasizing on the bronchial wall of lung cancer. The extent and pattern of infiltration along the proximal bronchial wall in the central type lung cancer were observed. The cancerous tissue extended proximally from less than 1.2 cm in the majority of cases, to a maximum of 1.6 cm from the cancer base. The lesions can be divided into three gross types: infiltrating, polypoid and nodular. In the infiltrating type, the cancerous tissue spread along the bronchial wall far and may involve multiple layers in the wall. In the polypoid type, the infiltration did not reach far as compared with the other types. None in the polypoid type was over 0.4 cm. The nodular type comprising 54% (27/50), was more common than the others. In order to improve the operative result, infiltrating pattern and distance along the bronchial wall are important references for resection of central type lung cancer.     

人工晶状体植入术中角膜后弹力层脱离的防治

为观察后房人工晶状体植入术中角膜后弹力层脱离发生原因和处理措施，在显微镜下对621例（643例）后房人工晶状体植入术病人在术中和术后进行了观察，并将后弹力层脱离分为4级记录。其中42眼发生不同程度的后弹力层脱离，发生率为6．53％。经正确处理后未发生严重并发症。认为手术中正确的切口位置和减少器械反复进入眼内是防止后弹力层脱离的重要因素，对高龄病人尤为重要。     

A sensitive fluorometric method for measurement of vascular permeability in spinal cord injury.

A sensitive fluorometric method was modified for the evaluation of drug action upon vascular permeability in rat spinal cord injury. Fluorescein isothiocyanate-conjugated dextran (FITC-D MW 71,200), used as a macromolecular tracer, was injected iv 2 hours before sacrifice. The optimal pH for FITC-D fluorescence was 8.2. The recovery in spinal cord was 101.4 +/- 4.0% (mean +/- SD). The extent of FITC-D extravasation, expressed as the vascular injury index (VII), was increased in proportion to the trauma force. The peak of VII after trauma was at 2 hours. This fluorometric method is sensitive, simple, and reliable for evaluation of drug effects upon vascular permeability in CNS trauma.     

湿温"三禁"辨析

湿温病历有禁汗、禁下、禁润"三禁"之说,前人所论固然有一定道理,但它绝不是一成不变之法,临证之际又必须灵活处理.当具体情况具体分析,"知犯何逆"而"随证治之",才是符合临床实际的辨证论治精神.     

Cancer mortality during the 1968-1994 period in a mining area in France.

We performed a geographical analysis of cancer mortality in the communes surrounding an industrial mining complex (Salsigne, France) where suspicious levels of pollution due to arsenic were measured. Compared with that observed in a control area, we showed a significant excess of mortality due to all cancer types (ratio of standard mortality ratios (ratio of SMRs)=1.1), lung cancer (ratio of SMRs=1.8), pharynx cancer (ratio of SMRs=2.1) in the whole population, and due to digestive system cancer (ratio of SMRs=1.3) among women. The results were similar after controlling for the occupation distribution in the populations. Excluding mining complex workers deaths from the deaths in the studied populations did not modify the pattern of our results. We concluded that the excess of cancer deaths could not be exclusively due to potential professional exposures among the workers of the mining complex and are probably explained by environmental contamination.     

人重组pcDNA6.2/生长分化因子5质粒的构建及鉴定

背景：生长分化因子5是一种刺激肌腱、韧带塑型，增强愈合反应有效的生长因子，在活性组织工程肌腱构建中有重要作用。 目的：构建人重组pcDNA6.2/生长分化因子5质粒，为转染基质干细胞向肌腱诱导分化实验奠定基础。 设计、时间及地点：细胞基因工程体外实验，于2007-08/12在哈尔滨医科大学遗传学教研室和分子生物学教研室完成。 材料：根据Genbank（NM000557）中人生长分化因子5的序列化学合成一对引物,从人胎儿软骨组织提取总RNA。 方法：通过反转录-聚合酶链反应得到人生长分化因子5完整成熟肽基因。将所得基因片段插入克隆载体pcDNA6.2并转化入JM109菌株，提取重组质粒，PCR鉴定、酶切鉴定并测序。 主要观察指标：反转录-聚合酶链反应检测结果，PCR及SalⅠ和BamHⅠ双酶切鉴定结果,重组质粒测序与Genbank中序列比较结果。 结果：电泳显示，反转录-聚合酶链反应产物为一长约380 bp的带，阳性克隆质粒经PCR电泳及双酶切均出现约380 bp的片段。测序表明与Genbank中的序列完全相符。 结论：成功构建出人重组pcDNA6.2/生长分化因子5质粒，为组织工程化肌腱过程中种子细胞的制备提供转染质粒。     

生理模型同时预报普鲁卡因胺及其代谢物乙酰普鲁卡因胺在大鼠体内处置动力学

用生理模型同时对iv普鲁卡因胺(PA)后,PA及其代谢产物乙酰普鲁卡因胺(NAPA)在大鼠体内处置动力学进行预报。测定了所需的有关PA参数.结果PA在大鼠血液、肝和肾脏清除率分别为47.28,13.56和33.71 ml·kg^-1·min^-1。估算的组织/血液药物浓度比表明,心、肝、肾、肌肉和小畅对PA的亲和力大于血液成分。对大鼠iv PA后,PA及其NAPA在组织中浓度进行预报,并与实验结果比较。结果大多数组织中浓度预报值与观察值基本—致。同时对PA及其NAPA在人血浆中浓度进行了预报。     

Pregnancy in patients on maintenance hemodialysis: seven cases report and 169 reported cases review

Objective To analyze and summarize the cases of pregnancy on maintenance hemodialysis (MHD), and review the literature. Methods Seven cases of pregnancy and childbirth in patients on MHD in the First Affiliated Hospital of Zhejiang University from Jan 2009 to Dec 2017 were analyzed, and the literature about pregnancy in patients on MHD reported in Pubmed and Web of Science database was retrieved. Both maternal and fetal outcome were studied. Results There are seven pregnant MHD patients in this center, among whom six patients went through a smooth pregnancy and one patient had intra-uterine fetal death at 14 weeks of pregnancy. The six patients had preterm labor. Among six fetuses, four grew in good health and developed well, one had physical development retardation and one had heart malformation at born. In the literature, 169 cases reported pregnant patients and 182 fetuses were evaluated, of which 145 live infants were delivered, 79.67% overall fetal survival rate, with gestational age of (32.94±3.34) weeks. In 164 delivered fetuses, 147 were preterm labor (89.63%). The weight of live births was (1824±609) g. There were no maternal deaths. Fetal survival rate was zero in＜20 weeks of gestational age, 20-24 weeks was 14.29%, 25-27 weeks was 45.45%, ≥28 weeks was 94.44%. The fetal survival rate was higher in pregnant woman receiving hemodialysis≥28 hours weekly compared to women receiving ＜16 hours weekly (92.31% vs 52.94%, P=0.02). Conclusions There are still a very high maternal and fetal complication rate in hemodialysis patients, especially in fetus. Gestational age≥28 weeks has a high fetal survival rate. Intensive dialysis during pregnancy may benefit higher fetal survival rate.     

Quantitative amino acid analysis using a Beckman system gold HPLC 126AA analyzer

BACKGROUND: The Beckman 6300/7300 analyzer, which was widely used for amino acid (AA) analysis, is no longer commercially available. METHODS: To set up an affordable AA analysis program, a Beckman system gold HPLC 126AA analyzer and Pickering Laboratories reagents were used. Two quantitative AA analysis programs were developed. One was an 18-min short program quantitating seven AAs from plasma and dried blood spots (DBS) specimens using Lithium eluents Li-365 and Li-375 at 70 degrees C column temperature. The short program could be used for diagnosis and follow-up dietary management for phenylketonuria (PKU), maple syrup urine disease (MSUD), tyrosinemia and homocystinuria patients. The second program was a 118-min long AA screening panel quantitating 40 AAs using Lithium eluents Li-275, Li-365 and Li-375 at 32, 48 and 72 degrees C column temperatures from plasma and urine specimens. RESULTS: The values obtained from DBS specimens were in good agreement with certified results from the Centers for Disease Control and Prevention. The values obtained from plasma and urine samples were in good correlation with those obtained from Beckman 6300 analyzer (0.9076 < or = r < or = 0.999). CONCLUSIONS: Amino acid quantitation from physiological samples using a Beckman 126AA Analyzer and Pickering Laboratories reagents was useful for clinical diagnosis and monitoring of aminoacidopathies.