细胞凋亡及增殖细胞核抗原在肝癌组织中的表达及意义

目的探讨经肝动脉化疗栓塞(TACE)后肝癌细胞中增殖细胞核抗原(PCNA)广凋亡细胞比值与肝细胞肝癌分化程度的关系。方法应用原位末端标记法(TUNEL)及免疫组化双染色技术，对60例肝细胞肝癌石蜡包埋组织进行检测。结果不同分化程度癌组织中细胞凋亡和PCNA的阳性率不同。分化程度高，细胞凋亡阳性率高、PCNA阳性率偏低；分化程度低，细胞凋亡阳性率偏低、PCNA阳性率高。随分化程度的降低，PCNA广凋亡细胞的比值显著增加，两者有相关性。结论PCNA/凋亡细胞的比值可作为评估肝细胞肝癌分化程度的指标。     

肿瘤坏死因子在妊高征发病中的作用

目的：探讨肿瘤坏死因子（ＴＮＦ）在妊高征发病中的作用及其对胎儿生长的影响。方法：应用放射免疫法对正常晚期妊娠妇女１６例（对照组）及妊高征患者４６例（妊高征组）的血浆、羊水和新生儿脐血中ＴＮＦ进行检测。结果：分娩前妊高征组血浆ＴＮＦ水平较对照组高，以中、重度妊高征者增高显著（Ｐ＜０．０５）；产后７２小时妊高征组血浆ＴＮＦ水平下降，与对照组差异无显著性。对照组及轻度妊高征者新生儿脐血ＴＮＦ水平与母血接近，羊水ＴＮＦ水平明显低于母血（Ｐ＜０．０５）；中、重度妊高征者脐血和羊水ＴＮＦ水平均较母血低（Ｐ＜０．０５；Ｐ＜０．０１）。新生儿脐血、羊水中ＴＮＦ水平在两组间差异无显著性。在中、重度妊高征者中，合并胎儿生长迟缓者其羊水和新生儿脐血ＴＮＦ水平明显高于未合并胎儿生长迟缓者。结论：ＴＮＦ可能作为母体对胎儿抗原的异常免疫反应的重要介质，在妊高征的发病中起一定作用。     

大鼠脑神经干细胞神经巢蛋白表达的流式细胞术检测

检测胎鼠及成年大鼠脑内神经干细胞特异性抗原神经巢蛋白的表达,判明啮齿类哺乳动物脑内神经干细胞存在的可能性及相应的部位。方法：显微手术下采取胎鼠及成年大鼠大脑与皮质下脑组织,间接荧光法标记ｎｅｓｔｉｎ,以流式细胞术检测。结果胎鼠脑内普遍存在ｎｅｓｔｉｎ阳性细胞,成年大鼠脑室旁组织中也存在ｎｅｓｔｉｎ阳性细胞。     

电视录相脑电监测在小儿癫痫诊断中的应用

目的评价电视录相脑电图（Ｖｉｄｅｏ－ＥＥＧ）监测在小儿癫痫诊断中的应用价值。方法对２０３例具有发作性症状的患儿进行连续６小时的包括清醒、睡眠、诱发试验及必要的认知测验的Ｖｉｄｅｏ－ＥＥＧ监测。结果经发作期Ｖｉｄｅｏ－ＥＥＧ监测证实，６０例初诊为癫痫性发作的患儿中，２１例（３５％）为非癫痫性发作；２８例其他症状发作中，２４例（８６％）为非癫痫性发作。１０２例痫样放电患儿中８２例（８０．４％）确定了发作类型，３５例（３４．３％）确定了癫痫类型。Ｖｉｄｅｏ－ＥＥＧ监测可发现短暂轻微的癫痫发作及痫样放电引起的一过性认知损伤的表现。结论Ｖｉｄｅｏ－ＥＥＧ在排除非癫痫性发作、确定癫痫性发作的类型、评价脑电－临床关系方面可提供准确可靠的证据，可进一步提高癫痫的临床诊断水平     

Focal cerebral ischemia alters the spatio-temporal properties, but not the amount of locomotor activity in mice

Cerebral ischemia induces hyperlocomotion in gerbils, and sensorimotor and cognition dysfunctions in rodents ; however little is known about the spatio-temporal organization of locomotor activity after ischemia. In this study, we continuously （22 h） assessed the spatio-temporal organization of locomotor activity in focal cerebral ischemic mice in an open field with feeding and drinking supplies. To observe the spatio-temporal changes,     

后基因组时代的胶质瘤分子生物学研究

在后基因组时代,对胶质瘤组织重构过程中的组织病理学、细胞遗传学及其相应分子改变的深入研究,为胶质瘤的分子病因、恶性进展、分子分型、基因治疗靶点筛选及预后评估研究提供有价值的参考依据.全文介绍了在该领域的相关研究进展.     

Effects of Electroacupuncture on the Number of NK-1R／IL-1β Double Labeled Immunofluorescent Positive Cells in th_e Epidermal and Subcutaneous Tissue Around the Ankle Joint of Adjuvant Monoarthritis in Rats

Adjuvant monoarthritis was induced by the injection of 50μl Complete Freund‘s Adjuvant（CFA) into ankle joint of adult SD rats. Electroacupuncture (EA)was performed on Huantiao (GB30)and Yanglingquan (GB 34) (0.5-1.5 v, 4-16 Hz, 30 min) after the injection.     

计算机网络系统在细菌耐药监测中的应用

目的计算机网络系统应用于医院细菌耐药监测的思路和措施.方法总结国内外细菌耐药监测的实践经验与相关文献报道.结果以局域网为基础,以互联网为目标,分级分批地建立监测系统是计算机网络系统的最终发展目标,而建立分级预警系统和标准化的抗菌药物评判知识库则是其成功运用的关键.结论数字化、电子化、智能化和人性化的细菌耐药监测系统是医院感染监测的必然发展趋势.     

Marked enhancement of anti-allodynic effect by combined intrathecal administration of the adenosine A1-receptor agonist R-phenylisopropyladenosine and morphine in a rat model of central pain

BACKGROUND: There is often no satisfactory treatment for chronic pain after spinal cord injury. We have previously reported that intrathecal (i.t.) administration of the adenosine A1-receptor agonist R-phenylisopropyl-adenosine (R-PIA) or the opioid morphine has anti-allodynic effects in a model of presumed chronic central pain after photochemically induced spinal cord injury in rats. In the present study, we set out to investigate the possible interaction between i.t. R-PIA and morphine in spinally injured rats. METHODS: Sprague-Dawley rats displaying allodynia-like behaviors to mechanical and cold stimuli after photochemically induced spinal cord injury with minor motor deficits were used. R-PIA and morphine, either alone or in combination, were administered i.t. through an implanted catheter to lumbar spinal cord. RESULTS: Cumulative doses of R-PIA or morphine dose-dependently reduced the mechanical allodynia-like behavior, with a threshold of 1 nmol and 1.5 nmol, respectively. When co-administrated, R-PIA and morphine produced marked suppression of mechanical allodynia at doses of 5 pmol and 7.5 pmol, respectively. The effect of i.t. co-administration of R-PIA and morphine on cold allodynia was comparable to i.t. R-PIA alone. The combination of R-PIA and morphine did not increase adverse effects such as motor deficits in comparison to either drug alone. CONCLUSION: These results demonstrate a supra-additive interaction between the adenosine A1-receptor agonist R-PIA and morphine to reduce mechanical allodynia-like behavior in rats with chronic spinal cord injury. The combination of R-PIA and morphine administered spinally may be superior to R-PIA or morphine alone for treating such pain.     

兔蜗神经直接动作电位的特征

目的　探讨新西兰大白兔蜗神经直接动作电位 (directcochlearnerveactionpotential,DCNAP)的记录方法和特征 ,建立术中听觉监护动物模型。方法　纯种新西兰大白兔 6只 (12耳 ) ,乙状窦后进路暴露小脑、脑干及双侧桥脑小脑角区 ,插入针状电极于蜗神经 ,记录DCNAP ,并按常规方法记录听性脑干反应 (auditorybrainstemresponse ,ABR)。结果　用本方法记录的DCNAP具有振幅大、波形稳定、重复性好、记录时间短的特点 ,但个体差异较大。结论　在蜗神经桥脑小脑角区用针状电极可以可靠记录到DCNAP ,是术中听觉监护的可行方法