宜宾市翠屏区“红层找水”水质卫生状况分析

目的了解宜宾市翠屏区"红层找水工程"农村生活饮用水卫生质量状况,为解决农村安全饮用水问题提供科学依据。方法对2007-2008年翠屏区修建的9 612口红层水井进行数量不少于3%的抽样检测,按照GB/T5750-2006《生活饮用水标准检验方法》进行检测,(GB5749-2006)《生活饮用水卫生标准》进行评价。结果共检测"红层找水工程"生活饮用水水样409份,合格率为19.3%,各项指标中合格率最低的是总大肠菌群,合格率仅为32.5%。各乡镇红层水井合格率差异有统计学意义(χ2=45.0,P﹤0.05),菜坝镇合格率最低。结论宜宾市翠屏区"红层找水工程"井水的水样合格率较低,水质较差,农村饮水安全问题十分突出,影响水质的主要因素是以微生物污染为主。     

正常眼压性青光眼诊断中存在的问题

患者具有典型的青光眼视盘损害、视野缺损、前房角开放、眼压正常，并排除眼部或全身性疾病引起的视神经病变，即可诊断为正常眼压性青光眼。准确的眼压测量、两次以上24小时眼压曲线描记以及对眼部和全身可能原发疾病的排除在诊断过程中至关重要。     

Effect of bee venom peptide-copolymer interactions on thermosensitive hydrogel delivery systems

The objectives of this study were to investigate the potential interactions between the model protein drug (bee venom peptide, BVP) and thermosensitive poly(dl-lactide-co-glycolide-b-ethyleneglycol-b-dl-lactide-co-glycolide) (PLGA-PEG-PLGA) copolymers and to examine the drug-copolymer interactions on the in vitro drug release and hydrogel degradation. The PLGA-PEG-PLGA copolymers were synthesized by ring-opening copolymerization of dl-lactide and glycolide with PEG as an initiator. Drug-copolymer co-precipitate blends were prepared and analyzed by Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) to characterize the specific interactions between drug and copolymer. For the better understanding the drug-copolymer interactions on drug release, insulin was selected for comparison. The release of the two protein drugs from the copolymer-based hydrogels and hydrogel degradation was studied at 37 degrees C under agitation. The results of FTIR and XRD indicated that the hydrogen bonding interactions existed between the NH group of BVP and CO group of the copolymers. The insulin and BVP released from the copolymer hydrogel over 15 and 40 days, respectively. The BVP-copolymer interactions retarded the BVP release rate and degradation of hydrogel, but did not significantly affect the biological activity of BVP. These results indicate that the drug-copolymer interactions need to be considered when attempting to use PLGA-PEG-PLGA hydrogels as sustained delivery carriers of protein or peptide drugs.     

硅油填充下牵引性视网膜脱离的手术治疗

目的总结硅油填充眼牵引性视网膜脱离在硅油下视网膜复位手术的疗效，探讨该手术的手术技巧及并发症的处理。方法观察19例(19只眼)硅油填充术后发生牵引性视网膜脱离患者，在保留硅油的条件下，做常规睫状体平坦部巩膜三切口，原灌注口接硅油灌注管，在导光纤维的导引下行膜剥离、视网膜增殖疤痕切开松解、内排液、补充硅油、眼内光凝等手术使视网膜复位的手术治疗。结果19只眼中，术后视网膜基本复位，术后发生一过性高眼压14例，前房、玻璃体出血2例，视网膜创伤疤痕增殖病灶过大，周边局部牵引性脱离复发2例。结论有硅油眼牵引性视网膜脱离在硅油填充下的膜剥离松解手术，具有方法简便、节省时间、剥膜止血容易等优点，是严重增殖性复发性视网膜脱离手术最终获得成功的关键过程。     

麦考酚酸产生菌液体发酵条件的优化

研究了短密青霉菌L产生麦考酚酸的发酵工艺条件，包括斜面培养基种类、初始pH、培养温度、碳氮源种类等，并通过均匀设计法优化了发酵培养基的组成。在优化条件下，摇瓶发酵单位达8565μg／ml，比优化前提高了89％。     

天冬混淆品(山文竹)的生药学检定

目的:对天冬混淆品(山文竹)进行生药学鉴定。方法:对山文竹原植物、生药性状、显微特征及理化特性等进行分析;并列表将天冬与山文竹进行对比鉴定。结果:二者原植物不同,生药性状、显微特征有明显区别,但化学成分定性鉴别基本相同。结论:山文竹能否作为天冬的代用品,有待进一步研究。     

十二指肠Brunner腺腺瘤的CT表现（附7例病例报道）

目的：探讨十二指肠Brunner腺腺瘤的CT影像学表现。方法回顾性分析7例经病理证实的十二指肠Brunner腺腺瘤CT表现。结果十二指肠Brunner腺腺瘤表现为密度均匀或不均匀的类圆形或长条形肿块,直径1．5～5．1 cm不等,平均4．1 cm,边缘清晰;CT平扫示肿瘤实体部分密度均与临近肠壁密度相似,增强扫描动脉期均呈中度不均匀强化,门静脉期均呈渐进性强化,密度仍欠均匀。结论十二指肠Brunner腺腺瘤的CT表现具有一定的特点,对其影像特征的认识可减少误诊率,但最终确诊仍有赖于病理诊断。     

腹腔镜辅助下胃癌根治术 71例临床报告

目的探讨腹腔镜辅助下胃癌根治术的安全性及可行性.方法 71例胃癌患者行腹腔镜辅助下根治性胃切除术,其中根治性全胃切除术 8例,近端胃大部切除术 16例,近端胃大部联合脾脏切除术 3例,远端胃大部切除术 44例.结果 71例患者,除 2例中转开腹外,其余 69例均成功进行腹腔镜手术.手术用时全胃切除 (343± 52)min,近端胃切除 (268± 62) min,近端胃切除联合脾脏切除 (312± 64) min,远端胃切除 (283± 44)min.术中出血量全胃切除 (267± 220)ml,近端胃切除 (150± 103)ml,近端胃联合脾脏切除 (333± 116)ml,远端胃切除 (139± 84)ml.清扫淋巴结 (34.3± 11.8)枚 /例.术后患者平均胃肠功能恢复时间 (4.1± 1.1)d,下床活动时间 (3.5± 1.0)d,进流质时间 (5.0± 1.2)d.术后近期效果良好.结论 腹腔镜胃癌根治术安全、可行,能够达到与开腹手术相当的根治效果,且具有创伤小、术后恢复快等优点.     

Fasudil inhibits tissue factor and plasminogen activator Inhibitor-1 secretion by peripheral blood mononuclear cells in CAPD patients

Disturbances in hemostasis are common complications of kidney diseases and correlate well with cardiovascular mortality. Little is known about the effects of fasudil on tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1) expression in peripheral blood mononuclear cells (PBMCs) in CAPD patients. PBMCs were isolated from 13 individuals with CAPD and 13 healthy subjects. After 4?h of incubation with or without LPS (10?ng/mL), TF and PAI-1 mRNA of PBMCs were detected by RT-PCR. The levels of TF and PAI-1 in culture supernatants of PBMCs were determined by ELISA. Compared with healthy controls, CAPD patients had increased TF, PAI-1 protein and mRNA expression by PBMCs at baseline and after stimulated by LPS (10?ng/mL) [p?<?0.001]. The fasudil treatment resulted in a significant effect in decreasing TF and PAI-1 [p?<?0.05] synthesis in PBMCs. TF and PAI-1 mRNA expression and activities in PBMCs were increased in CAPD patients. Fasudil reduced LPS-mediated TF and PAI-1 expression and activity in PBMCs. These effects may partially be relevant to the clinical benefits of fasudil in the treatment of CAPD patients.     

双侧睾丸间质细胞瘤1例报告并文献复习

目的 探讨睾丸间质细胞瘤的诊断和治疗经验.方法 报告1例双侧睾丸间质细胞瘤患者的临床资料并文献复习.结果 根据患者临床表现及相关检查,术前诊断考虑为双侧睾丸间质细胞瘤,术中快速冰冻病理检查示双侧睾丸间质细胞瘤,行睾丸肿瘤剜除术.术后随访6个月未见肿瘤复发或转移.结论 睾丸间质细胞瘤是一种较为罕见的睾丸肿瘤.术前诊断较为困难,睾酮、雌二醇等性激素水平升高及男性乳房发育等症状对睾丸间质细胞瘤的诊断有重要意义,确诊仍需依赖病理组织学检查.传统的手术方法采用根治性睾丸切除术,然而对于青春期前患者、双侧睾丸间质细胞瘤等患者,可以施行保留睾丸的肿瘤剜除术.