修复前关节囊在"肘关节恐怖三联征"手术治疗中的疗效观察

目的探讨修复前关节囊在"肘关节恐怖三联征"手术治疗中的疗效。 方法自2015年5月至2017年12月苏州大学附属瑞华医院手外科采用手术修复前关节囊治疗8例肘关节恐怖三联征患者，根据影像学评价观察骨折愈合情况。采用Mayo肘关节功能评分评估肘关节功能情况。 结果所有患者均获得6~36个月随访，平均18个月。切口均Ⅰ期愈合。骨折均愈合，时间为8~12周，平均10周。根据术后6个月随访，肘关节屈位0°~15°，伸位130°~145°，平均活动范围为115°，旋前60°~90°，旋后40°~70°，平均旋转范围120°。术后无骨折块移位、内固定失效、锁定接骨板螺钉松动或断裂、切口感染、异位骨化等并发症发生。肘关节功能恢复良好，采用Mayo肘关节功能评分：优6例，良2例。 结论在肘关节恐怖三联征时修复前关节囊，恢复肘关节的稳定性，并发症少，骨折愈合快，及早配合正规的康复锻炼，肘关节功能恢复好，疗效确切。     

Effect of C60 on the phase transition behavior of a lipid bilayer under high pressure

Interactions between fullerenes and cells and effects on the main transition of lipid bilayers have attracted much attention in biophysics in recent years. By employing coarse-grained molecular dynamics simulations, we obtained the temperature–pressure phase diagrams of a dipalmitoylphosphatidylcholine bilayer, which exhibits a gel phase and a fluid phase, with variation of the C₆₀versus lipid ratios. The simulation results show that the critical area per lipid at the fluid–gel main phase transition boundary increases with the increasing ratios of C₆₀. A critical area per lipid of 0.594 ± 0.002 nm² is obtained when the ratio of C₆₀ reaches 6.4% while that of the pure bilayer case is 0.572 ± 0.002 nm². The main transition temperature, T_m, remains almost unchanged with the addition of C₆₀ below a ratio of 4.7%, while a 2 K decrease of T_m is observed at a ratio of 6.4% under various pressures. Consequently, the presence of C₆₀ in the bilayer, with the ratio of C₆₀ less than 4.7%, will not influence the main transition behavior of the bilayer even under pressure as high as 1500 bar. The radial distribution function analyses suggest that the presence of C₆₀ produces no impact on the radial distribution of the lipids in the bilayers. The lateral density profiles show that the incorporation of C₆₀ with relatively high ratios stabilizes the thickness of the bilayer.

By employing coarse-grained molecular dynamics simulations, we obtained the temperature–pressure phase diagrams of a dipalmitoylphosphatidylcholine bilayer, which exhibits a gel phase and a fluid phase, with variation of the C₆₀versus lipid ratios.      

In vivo detection of salicylic acid in sunflower seedlings under salt stress

Salicylic acid (SA) is an important phytohormone. It plays an essential role in regulating many physiological processes of plants. Most of the conventional methods for SA detection are based on in vitro processes. More attention should be paid to develop in vivo methods for SA detection. In this work, Pt nanoflowers and GO were simultaneously electrodeposited and reduced on a Pt wire microelectrode in one step. The Pt nanoflowers/ERGO modified Pt microsensor demonstrated high sensitivity and selectivity for SA. SA could be detected from 100 pM to 1 μM with a detection limit of 48.11 pM. Then this microsensor was used to detect SA in the stem of sunflower seedlings under different salt stresses in vivo. The result showed that with the increasing concentration of salt, SA levels decreased. Our result was also confirmed by UPLC-MS and gene expression analysis. To the best of our knowledge, this is the first report of in vivo detection of SA in plants using the Pt nanoflowers/ERGO modified Pt microelectrode. It is foreseeable that our strategy could pave the way for the in vivo detection of phytohormones in plants.

A Pt nanoflowers/ERGO modified Pt microelectrode was proposed to detect salicylic acid in plants under salt stress in vivo.     

磁共振动脉自旋标记全脑容积灌注成像对短暂性脑缺血发作的诊断价值

探讨动脉自旋标记全脑容积灌注成像（3D-ASL)对短暂性脑缺血发作(TIA)的诊断价值。对48例临床诊断短暂性脑缺血发作的患者据最后一次发作24 h内行磁共振常规扫描（包含T1WI、T2WI、T2-FLAIR、DWI序列)、磁共振血管成像（3D-TOF-MRA)及3D-ASL扫描,并根据扫描结果对48例短暂性脑缺血发作患者进行分析。常规平扫检出异常14例（29.17%）;3D-MRA检出异常18例(37.50%);3D-ASL检出异常37例(77.09%)。动脉自旋标记全脑容积灌注成像对短暂性脑缺血发作的检出率远高于常规平扫及血管成像,有显著性差异,且简便易行,可以作为诊断短暂性脑缺血发作的常规检查方法予以推广。     

用SCGE法观察砷、氟、黄绿青霉素和T-2毒素引起的SGC-7901细胞基因组DNA损伤

目的观察砷、氟、黄绿青霉素和T-2毒素对SGC-790l细胞基因组DNA的损伤作用及特点。方法染毒剂量lOμmol／L，染毒后4h应用单细胞凝胶电泳技术结合“彗星图像分析系统”检测DNA损伤。结果NaAsO2组尾长明显大于对照组；NaF组拖尾率显著高于对照组；CIT组总拖尾率、尾长和尾动量均高于对照组；而T-2毒素组各指标均高于对照组。结论NaAsO2、NaF、CIT和T-2毒素均可引起DNA损伤，但各有特点，说明它们引起DNA损伤的机制可能是不同的。     

多聚物型可吸收冠状动脉支架的研究进展

新一代药物洗脱支架由可吸收多聚物为平台,具有与金属支架相当的的支撑力,以及良好的生物相容性.既可以暂时支撑管壁,抑制早期血栓形成及晚期新生内膜增生,还可作为良好的药物载体实现局部控制释放,达到有效防止支架置入后的血管急性闭塞和降低再狭窄率.     

舒芬太尼靶控输注对非体外循环冠状动脉旁路移植术患者麻醉效果的影响

目的:比较不同效应室浓度的舒芬太尼靶控输注对非体外循环冠状动脉旁路移植术(OPCAB)患者麻醉效果的影响。方法:42例择期OPCAB的患者随机分为3组,分别在术中使用0.4ng/mL(组Ⅰ)、0.6ng/mL(组Ⅱ)和0.8ng/mL(组Ⅲ)固定效应室浓度的舒芬太尼靶控静脉麻醉。观察患者术中血流动力学及脑电双频普指数(BIS)值,记录术中舒芬太尼、丙泊酚、多巴胺用量,术后恢复时间及围手术期心肌酶谱[肌酸激酶(CK)与肌酸激酶同工酶(CK-MB)]。结果:3组患者术中都能维持满意的血流动力学。术中丙泊酚、多巴胺的用量,术后恢复时间和围手术期心肌酶谱差异无显著性(P>0.05)。但Ⅱ组与Ⅲ组患者舒芬太尼用量多于Ⅰ组(P<0.01),Ⅲ组患者心脏指数(CI)在手术开始时低于Ⅰ组(P<0.05),心率(HR)自搭桥开始后增快(与搭桥前比较P<0.01),Ⅰ组患者术中BIS值高于Ⅱ组与Ⅲ组(P<0.05)。结论:采用0.4ng/mL、0.6ng/mL或0.8ng/mL固定效应室浓度的舒芬太尼靶控静脉麻醉对OPCAB患者术中均可以维持满意的血流动力学,但0.6ng/mL为最合适的靶控浓度。     

前列腺癌CK5^+CK8^+细胞的分选及其分子生物学特性

目的在人前列腺癌细胞系中分选CK5^+CK8^+细胞,了解其分子生物学特性。方法流式细胞术在人前列腺癌细胞系PC3和LNCaP中分选CK5^+CK8^+细胞,用实时荧光定量聚合酶链反应(RT-PCR)和蛋白质印迹法方法检测LNCaP细胞分选的CK5^+CK8^+细胞CK5、CK8、雄激素受体(AR)和前列腺特异抗原(PSA)的表达,细胞迁移实验检测CK5^+CK8^+细胞迁移能力,琼脂糖凝胶克隆形成实验检测CK5^+CK8^+细胞成瘤能力。采用t检验进行统计学分析。结果采用流式细胞术可以在PC3中分选出(21.3±4.6)%的CK5^+CK8^+细胞,在LNCaP中分选出(1.2±0.4)%的CK5^+CK8^+细胞。与非CK5^+CK8^+细胞相比,LNCaP中分选的CK5^+CK8^+细胞CK5 mRNA表达差异有统计学意义(t=10.435,P<0.001),CK8 mRNA表达差异无统计学意义(t=1.974,P=0.121),AR和PSA mRNA表达差异有统计学意义(t=4.317,3.232;P=0.016,0.037)。蛋白质印迹法检测得到类似结果。细胞培养7 d后,CK5^+CK8^+细胞和非CK5^+CK8^+细胞均可以在Transwell小室迁移生长,迁移细胞数分别为(60±7)个和(32±5)个,2者比较差异有统计学意义(t=6.031,P=0.004)。细胞培养14 d后,CK5^+CK8^+细胞和非CK5^+CK8^+细胞均可以在软琼脂糖凝胶中克隆性生长,阳性克隆数分别为(71±5)个和(27±3)个,差异有统计学意义(t=13.009,P<0.001)。结论人前列腺癌细胞系LNCaP和PC3都可以分选出CK5^+CK8^+细胞,LNCaP中CK5^+CK8^+细胞AR和PSA均有一定程度表达,迁移和成瘤能力增强。