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971.
Ehrmann IE; Ellis PS; Mazeyrat S; Duthie S; Brockdorff N; Mattei MG; Gavin MA; Affara NA; Brown GM; Simpson E; Mitchell MJ; Scott DM 《Human molecular genetics》1998,7(11):1725-1737
The Delta Sxrb interval of the mouse Y chromosome is critical for
spermatogenesis and expression of the male-specific minor transplantation
antigen H-Y. Several genes have been mapped to this interval and each has a
homologue on the X chromosome. Four, Zfy1 , Zfy2 , Ube1y and Dffry , are
expressed specifically in the testis and their X homologues are not
transcribed from the inactive X chromosome. A further two, Smcy and Uty ,
are ubiquitously expressed and their X homologues escape X-inactivation.
Here we report the identification of another gene from this region of the
mouse Y chromosome. It encodes the highly conserved eukaryotic translation
initiation factor eIF-2gamma. In the mouse this gene is ubiquitously
expressed, has an X chromosome homologue which maps close to Dmd and
escapes X-inactivation. The coding regions of the X and Y genes show 86%
nucleotide identity and encode putative products with 98% amino acid
identity. In humans, the eIF-2gamma structural gene is located on the X
chromosome at Xp21 and this also escapes X-inactivation. However, there is
no evidence of a Y copy of this gene in humans. We have identified
autosomal retroposons of eIF-2gamma in both humans and mice and an
additional retroposon on the X chromosome in some mouse strains. Ark blot
analysis of eutherian and metatherian genomic DNA indicates that X-Y
homologues are present in all species tested except simian primates and
kangaroo and that retroposons are common to a wide range of mammals. These
results shed light on the evolution of X-Y homologous genes.
相似文献
972.
Disruption of the clathrin heavy chain-like gene (CLTCL) associated with features of DGS/VCFS: a balanced (21;22)(p12;q11) translocation 总被引:5,自引:2,他引:5
973.
Ali RR; Reichel MB; Thrasher AJ; Levinsky RJ; Kinnon C; Kanuga N; Hunt DM; Bhattacharya SS 《Human molecular genetics》1996,5(5):591-594
Gene transfer to photoreceptor cells may provide a means for arresting the
retinal degeneration that is characteristic of many inherited causes of
blindness, including retinitis pigmentosa (RP). However, transduction of
photoreceptors has to date been inefficient, and further limited by
toxicity and immune responses directed against vector-specific proteins. An
alternative vector system based on adeno- associated virus (AAV) may
obviate these problems, and may be useful for transduction of neuronal
cells. In this study we have demonstrated successful transduction of all
layers of the neuroretina as well as the retinal pigment epithelium (RPE)
following subretinal injection of recombinant AAV particles encoding lac Z.
Furthermore, the efficiency of transduction of photoreceptors is
significantly higher than that achieved with an equivalent adenoviral
vector. This is the first report showing that AAV is capable of transducing
photoreceptor cells and supports the use of this vector system for gene
therapy of retinal diseases such as RP.
相似文献
974.
Anoop T. M. MD DM DNB Rona Joseph P. MD DM Anju Anna Abraham MD DM Mintu Mathew MD Bhavya S. Kumar MD DNB 《The breast journal》2021,27(11):811-816
FinHer regimen is considered a relatively cardiac safe regimen for Her 2 positive breast cancer in resource-limited settings. There is limited data on cardiotoxicity of this regimen. Out of 1200 patients diagnosed with carcinoma breast during the study period, three hundred Her2-positive early–breast cancer patients received FinHer protocol were included. Among the 300 patients, a total of 71 patients (24%) experienced cardiac toxicity including asymptomatic EF loss in 62 patients (21%) and symptomatic LVEF loss in nine patients (3%). Among patients with symptomatic LVEF loss, six patients had symptomatic cardiac toxicity, one patient (0.3%) had symptoms with fall in EF after completion of treatment, one patient (0.3%) had Congestive cardiac failure (CHF); one patient (0.3%) had non-ST elevation myocardial infarction (NSTEMI). Later, trastuzumab was rechallenged in all 62 patients (24%) with asymptomatic LVEF loss and six patients (2%) with symptomatic LVEF loss. One patient with CHF and NSTEMI was not rechallenged. Hypertension and diabetic mellitus which were the two factors found to have risk on univariate logistic regression analysis although it was not statistically significant. None of these patients further experienced cardiac toxicity at 24 months follow-up except one patient. Although FinHer protocol is considered a cardiac safe protocol, cardiotoxicity associated with trastuzumab which can manifest as an asymptomatic decline in LVEF is more than usually expected in a real-world scenario. 相似文献
975.
In the new NHS those who provide services for disabled children need to measure and demonstrate their effectiveness, but there are no easily available outcome measures for use by child development centres and teams. The development of an alternative approach, using a series of statements of good practice, is described. Parents of children with cerebral palsy were asked to participate in semistructured interviews, to ascertain the value and relevance of these quality statements. Parents were most concerned about the standard of news breaking and early follow up, the sharing of information, and the supply and repair of equipment. The findings were used to modify the quality checklist and it is proposed that this should form the basis of a "charter for disabled children and their families'. 相似文献
976.
PS SUKTHANKAR BN BORGHAIN DM PARIKH RS RAO VH DESHMANE HK PARIKH 《Medical Journal Armed Forces India》1998,54(1):3-5
One hundred and sixty five patients, treated with carbondioxide (CO2) laser for benign and malignant lesions of the head and neck were studied. Alveolo-buccal complex (68/165) had the majority of benign and malignant tumors followed by larynx (23/165) and tongue (21/165). All lesions were widely excised, none were reconstructed and all defects healed well with minimal scarring. The post-operative morbidity was minimal and the hospitalization period was 1 to 5 days. Complications were not serious and could be managed easily. This study confirms the usefulness of CO2 laser surgery for both benign and malignant conditions in head and neck.KEYWORDS: Carbondioxide laser, Head and neck, Malignant neoplasm, Premalignant lesions 相似文献
977.
Modern use of clomiphene citrate in induction of ovulation 总被引:11,自引:1,他引:11
Clomiphene citrate is the treatment of first choice in the managementof infertility in normally oestrogenized, anovulatory women(WH0 group II). The majority of women with 'pure' anovulatoryinfertility respond to treatment with clomiphene citrate. Therates of pregnancy and miscarriage are close to those expectedin a normal fertile population. Basal hormone concentrationsdo not predict outcome. An increased body mass index is theonly factor which is consistently associated with a decreasedresponse to clomiphene citrate; it follows therefore, that weightreduction should be an important part of therapy in anovulatorywomen. According to our data, only an increased luteinizinghormone value immediately post clomiphene citrate predictedan adverse pregnancy outcome in women who conceived. Clomiphenecitrate, along with other ovulation induction therapies, cancause multiple follicular development, with a risk of ovarianhyperstimulation and multiple pregnancy. Ultrasound monitoringof treatment is important in order to choose the appropriatedose of clomiphene citrate in subsequent cycles and to minimizethe risks of hyperstimulation and multiple pregnancy. When coupleswith other factors contributing to subfertility are excluded,the cumulative conception rate continues to rise after 6 monthsof treatment with clomiphene citrate, reaches a plateau by treatmentcycle 12 and approaches that of the normal population. It hasbeen reported that prolonged use of clomiphene citrate may beassociated with an increased risk of a borderline or invasiveovarian tumour. Taking into consideration these observations,we recommend that anovulatory women responsive to clomiphenecitrate should be treated for at least 6 cycles before consideringmore complex or invasive methods of ovulation induction, andthat treatment should probably be limited to a maximum of 12cycles. 相似文献
978.
Kelsell RE; Evans K; Gregory CY; Moore AT; Bird AC; Hunt DM 《Human molecular genetics》1997,6(4):597-600
We have performed genetic linkage analysis on a four generation British
family with cone-rod dystrophy. Significant linkage to the disease gene was
obtained with eight marker loci situated on chromosome 17p12-p13. A maximum
two-point lod score of 5.93 with no recombination was obtained with marker
locus D17S1844. Critical recombinants identified with flanking marker loci
placed the disease gene between D17S796/D17S938 and D17S954, an interval
estimated to be 8 cM in size. This new localisation for autosomal dominant
cone-rod dystrophy (CORD6) overlaps with regions attributed previously to
Leber's congenital amaurosis, central areolar choroidal dystrophy and
dominant cone dystrophy. Given their differences in phenotype, the most
plausible explanation would be that these different retinal disorders are
caused by mutations in different genes mapping close together within the
genome.
相似文献
979.
目的 建立并评价电子束CT (EBT)两种方法在冠状动脉搭桥术后 (CABG)随访应用中的价值。方法 本组共 2 14例CABG患者进行了EBT两种方法的扫描 :(1)电子束CT血管造影 (EBA) ,以完成冠状动脉及搭桥血管的三维重建 ;(2 )电子束CT血流扫描 ,以得到冠状动脉及其搭桥血管的血流曲线。结果 本组共分析了 589条冠状动脉搭桥血管 (10条血管因图像伪影而除外 )。 133条动脉材料的血管桥通畅率超过静脉桥 (P <0 0 0 1)。三维重建方法诊断的敏感性、特异性和准确性分别是 97 7%、94 1%和 96 7% ,均高于血流扫描方法 (分别是 88 4 %、82 4 %和 85 2 % )。动脉与静脉血管桥内的平均血流量分别为 4 9± 2 2ml·min 1·g 1和 6 9± 2 8ml·min 1·g 1(P <0 0 0 1)。结论 电子束CT血管造影配合三维重建方法有利于观察冠状动脉及其搭桥血管的解剖 ,而血流扫描方法有利于观察并定量评估搭桥血管内的血流 ,两种方法的结合是理想的冠状动脉搭桥术后随访的无创影像方法 相似文献
980.
Recent epidemiological studies have identified plasma triglyceride as a
risk factor for atherogenesis. We have generated a mouse transgenic line
that carries a recessive mutation designated lpd (lipid defect). Homozygous
lpd mice develop as runts and die by age 10-15 days with striking liver
pathology characterized by the presence of numerous lipid-containing
vacuoles and extensive accumulation of triglycerides. Cloning of the mutant
insertion locus and the wild-type lpd locus have revealed a duplication of
host genomic sequences at the site of integration. Mapping of the lpd locus
with the Jackson Laboratory BSS interspecific backcross panel of
(C57BL/6JEi x SPRET/Ei) F1 x SPRET/Ei placed the lpd locus to the distal
part of chromosome 16. These observations suggest that the transgene
disrupts a putative gene at the lpd locus and that lpd is a novel locus
related to triglyceride metabolism. The lpd mutant mice may serve as models
for human disorders of fatty livers or hypertriglyceridemia.
相似文献