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91.
Effect of low-dose prednisone (with calcium and calcitriol supplementation) on calcium and bone metabolism in healthy volunteers 总被引:2,自引:0,他引:2
Lems WF; Van Veen GJ; Gerrits MI; Jacobs JW; Houben HH; Van Rijn HJ; Bijlsma JW 《Rheumatology (Oxford, England)》1998,37(1):27-33
The administration of moderate to high doses of corticosteroids is
associated with bone loss. This probably results from the uncoupling of
bone formation (decreased) and bone resorption (unchanged or increased). We
examined the effect of low-dose (10 mg/day) prednisone (LDP) and the
possible mitigating effects of calcium and 1.25 (OH)2 vitamin D
(calcitriol) on calcium and bone metabolism in eight healthy, young male
volunteers. The study consisted of four observation periods: in the first
period, LDP was prescribed during 1 week; in the second, third and fourth
periods, calcium (500 mg/day), calcitriol (0.5 micrograms b.i.d.) and
calcium in combination with calcitriol, respectively, were added to LDP.
Bone formation was measured by means of serum osteocalcin, carboxy-terminal
propeptide of type 1 procollagen (P1CP) and alkaline phosphatase, bone
resorption by means of urinary excretion of calcium, hydroxyproline, (free
and total) pyridinoline, (free and total) deoxypyridinoline and serum
carboxy-terminal cross- linked telopeptide of type 1 collagen (1CTP).
Dietary calcium and sodium intake were maintained at a stable level during
the entire study period. Treatment with LDP led to a decrease in
osteocalcin, P1CP and alkaline phosphatase (all P < 0.01). Urinary
excretion of pyridinolines, hydroxyproline and serum 1CTP did not increase,
but remained unchanged or slightly reduced (P < 0.05), depending on the
time of measurement and the marker of bone resorption. Parathyroid hormone
(PTH) (insignificantly) increased during LDP (+19%) and LDP plus calcium
(+14%), but decreased during supplementation with calcitriol (-16%) and
calcium/calcitriol (-44%; P < 0.01). Urinary excretion of calcium
increased during treatment with LDP and calcitriol (P < 0.05) and
calcium/calcitriol (P < 0.05). It is concluded that LDP has a negative
effect on bone metabolism, since bone formation decreased while bone
resorption remained unchanged or decreased slightly. The increase in PTH
during LDP could be prevented by calcitriol combined with calcium
supplementation.
相似文献
92.
Early recurrence or persistence of autoimmune diseases after unmanipulated autologous stem cell transplantation 总被引:20,自引:2,他引:20
Euler HH; Marmont AM; Bacigalupo A; Fastenrath S; Dreger P; Hoffknecht M; Zander AR; Schalke B; Hahn U; Haas R; Schmitz N 《Blood》1996,88(9):3621-3625
Autologous stem cell transplantation with or without in vitro lymphocyte depletion has been suggested as a new treatment option for severe autoimmune diseases. We describe five patients with autoimmune diseases (CREST syndrome, myasthenia gravis and Hashimoto's thyroiditis, systemic lupus erythematosus, atopic dermatitis, and rheumatoid arthritis) who underwent autologous bone marrow (n = 1) or peripheral blood progenitor cell (n = 4) transplantation with unmanipulated grafts as treatment for the autoimmune disease in one case or as treatment for a malignant disorder with a concomitant autoimmune disorder in four cases. In all patients serological and clinical signs of the autoimmune disease recurred early or persisted. These observations should be regarded as a cautionary note concerning the efficacy of high-dose therapy followed by transplantation of unmanipulated autologous stem cells for treatment of severe autoimmune diseases. 相似文献
93.
Mohammad-Khani S Otremba B Klein R Capelle HH Logemann F Bange FC Schmidt RE Stoll M 《European journal of medical research》2010,15(11):504-506
Cryptococcus neoformans is the most common cause of life threatening meningoencephalitis in HIV-infected patients. Diagnosis is based on tests for cryptoccocal antigen in serum and cerebrospinal fluid, and on culture of the organism. We present a case of AIDS-related cryptococcal meningoencephalitis unresponsive to antifungal combination therapy, despite of evidence of fungal susceptibility in vitro. Significant decreases in cryptococcal antigen titers in serum and cerebrospinal fluid did not correlate with progress in disease and fatal outcome. 相似文献
94.
Variation in the composition of chromatin has been proposed to generate a 'histone code' that epigenetically regulates gene expression in a variety of eukaryotic systems. As a result of the process of X chromosome inactivation, chromatinon the mammalian inactive X chromosome (Xi) is marked by several modifications, including histone hypoacetylation, trimethylation of lysine 9 on histone H3 (H3TrimK9) and substitution of core histone H2A with the histone variant MacroH2A. H3TrimK9 is a well-studied marker for heterochromatin in many organisms, but the distribution and function of MacroH2A are less clear. Cytologically, the Xi in human cells comprises alternating and largely non-overlapping approximately 10-15 Mb domains marked by MacroH2A and H3TrimK9. To examine the genomic deposition of MacroH2A, H3TrimK9 and acetylated histone H4 modifications on the Xi at higher resolution, we used chromatin immunoprecipitation in combination with a SNP-based assay to distinguish the Xi and active X (Xa) in a diploid female cell line and to determine quantitatively the relative enrichment of these histone code elements on the Xi relative to the Xa. Although we found a majority of sites were enriched for either MacroH2A or H3TrimK9 in a manner consistent with the cytological appearance of the Xi, a range of different histone code types were detected at different sites along the X. These findings suggest that the nature of the heterochromatin histone code associated with X inactivation may be more heterogeneous than previously thought and imply that gene silencing can be achieved by a variety of different epigenetic mechanisms whose genomic, evolutionary or developmental basis is now amenable to investigation. 相似文献
95.
Renée J Detollenaere Jan den Boon Jelle Stekelenburg Akeel HH Alhafidh Robert A Hakvoort Mark E Vierhout Hugo WF van Eijndhoven 《BMC women's health》2011,11(1):4
Background
Pelvic organ prolapse is a common health problem, affecting up to 40% of parous women over 50 years old, with significant negative influence on quality of life. Vaginal hysterectomy is currently the leading treatment method for patients with symptomatic uterine prolapse. Several studies have shown that sacrospinous fixation in case of uterine prolapse is a safe and effective alternative to vaginal hysterectomy. However, no large randomized trials with long-term follow-up have been performed to compare efficacy and quality of life between both techniques. 相似文献96.
Bruysters M Pertz HH Teunissen A Bakker RA Gillard M Chatelain P Schunack W Timmerman H Leurs R 《European journal of pharmacology》2004,487(1-3):55-63
Histaprodifens constitute a new class of histamine H(1)-receptor agonists. These ligands can be regarded as hybrid molecules, consisting of a histamine moiety linked at the two-position of the imidazole ring by a propyl chain to two phenyl rings, one of the characteristic features of several H(1)-receptor antagonists. To delineate the binding site of various histaprodifen-like ligands, we generated mutant histamine H(1) receptors, in which various amino acids, involved in the binding of either histamine or H(1)-receptor antagonists, were replaced by alanine. Wild-type and mutant H(1) receptors were transiently expressed in African green monkey kidney cells (COS-7) and evaluated for their interaction with histamine and various histaprodifens by [(3)H]mepyramine radioligand-binding studies and by nuclear factor kappaB (NF-kappaB) reporter-gene assays. Our data show that, within the histamine H(1)-receptor binding pocket, histaprodifens interact with both agonist and antagonist binding sites, resulting in high affinity histamine H(1)-receptor agonists. 相似文献
97.
进展期胃癌淋巴结转移与临床病理特征的关系 总被引:3,自引:1,他引:3
目的:探讨进展期胃癌淋巴结转移与临床病理特征的关系,为临床上进行合理的淋巴结清扫提供依据。方法:对55例进展期胃癌资料进行回顾性分析,术后常规解剖原发灶及各组淋巴结,并标记和计数,分析肿瘤部位、肿瘤大小、浸润深度、分化程度及Lauren分型与淋巴结转移率的关系。结果:进展期胃癌淋巴结转移率为74.5%;U、M、L区及全胃癌淋巴结转移率为85.7%、87.5%、67.6%和83.3%,各区和全胃癌淋巴结转移率差异无统计学意义(P〉0.05);浆膜受侵的胃癌淋巴结转移率为82.5%,明显高于浆膜未受侵者(53.3%)(P〈0.05);弥漫型胃癌淋巴结转移率为83.3%,明显高于肠型(64.0%)(P〈0.05);直径〉5cm癌灶淋巴结转移率为90.0%,明显高于直径≤5cm的胃癌患者(65.7%)(P〈0.05);浸润深度、Lauren分型和肿瘤大小是影响淋巴结转移率的主要因素,其中浸润深度为独立影响因素。结论:术中淋巴结清扫范围应结合肿瘤部位、肿瘤大小、浸润深度、分化程度及Lauren分型做出判断,并考虑患者的全身情况,合理选择淋巴结清扫范围。 相似文献
98.
Synergism of atenoloi and nitrendipine on hemodynamic amelioration and organ protection in hypertensive rats 下载免费PDF全文
OBJECTIVE: This study was designed to investigate the possible synergism of atenolol and nitrendipine on blood pressure (BP) and blood pressure variability (BPV) reductions, baroreflex sensitivity (BRS) amelioration, and organ protection in hypertensive rats. METHOD: The dose was 20 mg/kg for atenolol, 10 mg/kg for nitrendipine and 20 + 10 mg/kg for the combination of these two drugs. In an acute study, a single dose was given via a catheter previously inserted into the stomach in spontaneously hypertensive rats (SHR). 相似文献
99.
建立测定复方红甲凝胶中乳糖酸红霉素的含量测定方法。方法:以一阶导数光谱的谷一零位值法测定乳糖酸红霉素的含量,测定波长λ谷=490±lnm。结果:回收率100.6%,RSD为3.1%,线性范围40~120μg/ml。结论:方法简便,结果准确,重现性好,适合于该制剂的含量测定。 相似文献
100.