Activity of lymphocytes and antibodies derived from aging and adult rats bearing a MC-induced tumor in the syngeneic ADCC test

The purpose of the present paper was to demonstrate the antitumor activity of lymphocytes and antibodies in the antibody-dependent cell-mediated cytotoxicity test (ADCC) in relation to the tumor growth in adult and aging rats bearing a methylcholanthrene-induced sarcoma (MC-Sa). In the syngeneic system using the ADCC test effector spleen lymphocytes, 51Cr labeled MC-Sa target cells and anti-MC-Sa serum were derived from inbred male Wistar rats. It was found that adult and aging tumor-bearing rats possessed lymphocytes (LSa) and antibodies (Ab) which were active in ADCC. In comparative studies between the groups of adult and aging rats a reverse relationship between tumor growth and activity of LSa and Ab in the ADCC test has been found. Thus, the larger weight of tumors in the group of adult rats was followed by lower LSa and Ab activities in the ADCC test. On the other hand, the smaller tumor growth in the group of aging rats was followed by higher activities of LSa and Ab in this test. We suggest that the ADCC phenomenon could be one of the antitumor mechanisms especially effective in aging rats.     

Pseudostenokardie-Symptomkomplexe bei hämodynamischer Diagnostik bei Herzischämie

The purpose of this study was to determine the frequency of pseudocoronary syndromes in persons undergoing cardiac diagnostic tests because of suspected ischaemic heart disease. The study involved 384 subjects, 114 women and 270 men aged 21–75 years, who had been referred for precise cardiac diagnosis with a tentative preliminary diagnosis of ischaemic heart disease. In each subject an exercise stress test was performed, as was an ECG both at rest and during exercise and a coronary angiogram. Subjective sensations (e.g. pain in the chest, dyspnoea) experienced during the tests were also taken into account. Of the 384 patients suffering from pain in the chest area, ischaemic heart disease was excluded in 180 cases. In this subgroup the target HR (submaximal HR) was achieved during the stress test without any significant alterations in the ECG trace (raising or lowering of the ST segment). The subsequent coronary angiography definitively excluded any cardiogenic origin of the pain in the chest.     

ACTH in the plasma and cerebrospinal fluid in patients with multiple sclerosis

In 1984 and at the beginning of 1985 the authors carried out radioimmunoassays (SORIN-CIS kit) the plasma levels of ACTH in 116 multiple sclerosis patients (m-52, f-64) and in 10 cases this radioimmunoassay was done in the cerebrospinal fluid (m-5, f-5). The control group comprised 90 patients with ischialgia and neuroses. The normal value in the plasma was from 0 to 80.86 pg/ml, and in the fluid it was from 0 to 77.08 pg/ml. In multiple sclerosis patients the plasma ACTH level was from 0 to 286.9 pg/ml, in the cerebrospinal fluid from 0 to 89 pg/ml. The values of ACTH were significantly higher in multiple sclerosis patients, mainly in males. In the fluid the level of ACTH was significantly higher in the studied patients. No significant differences in ACTH levels were found between males and females with multiple sclerosis, and in the control group this level was higher in females. Raised ACTH level was found mainly in multiple sclerosis with lung duration of the disease (10 years) at the time of exacerbations. The authors continue studies on the axis hypothalamus-hypophysis-adrenals, on various hormones, prostaglandins, beta-endorphin, biochemical markers, cAMP, cCMP, arylosulphatase A and B MBC etc.     

Bromodeoxyuridine Labeling Index as an Indicator of Early Tumor Response to Preoperative Radiotherapy in Patients with Rectal Cancer

Purpose Assessment of tumor proliferation rate using Bromodeoxyuridine labeling index (BrdUrdLI) as a possible predictor of rectal cancer response to preoperative radiotherapy (RT). Methods and material Ninety-two patients were qualified either to short RT (5 Gy/fraction/5 days) and surgery about 1 week after RT (schedule I), or to short RT and 4–5 weeks interval before surgery (schedule II). Tumor samples were taken twice from each patient: before RT and at the time of surgery. The samples were incubated with BrdUrd for 1 h at 37°C, and the BrdUrdLI was calculated as a percentage of BrdUrd-labeled cells. Results Thirty-eight patients were treated according to schedule I and 54 patients according to schedule II. Mean BrdUrdLI before RT was 8.5% and its value did not differ between the patients in the two compared groups. After RT tumors showed statistically significant growth inhibition (reduction of BrdUrdLI). As the pretreatment BrdUrd LI was not predictive for early clinical and pathologic tumor response, prognostic role of the ratio of BrdUrdLI after to BrdUrdLI before RT was considered. The ratios were calculated separately for fast (BrdUrd LI > 8.5%) and slowly (BrdUrd LI ≤ 8.5%) proliferating tumors and correlated with overall treatment time (OTT, i.e., time from the first day of RT to surgery). One month after RT, accelerated proliferation was observed only in slowly proliferating tumors. Conclusions Pretreatment BrdUrdLI was not predictive for early clinical and pathologic tumor response. The ratio after/before RT BrdUrdLI was correlated to inhibition of proliferation in responsive tumors. The paper was presented at ECCO 13, October 30 to November 03, 2005 in Paris, France     

Tau isoform expression in frontotemporal dementia without tau deposition

In many cases of sporadic frontotemporal dementia (FTD) and in FTD caused by tau mutations (FTDP-17) there is disruption of the normal splicing of tau leading to the aberrant expression of tau isoforms and neurodegeneration. This suggests a central role for tau in the pathogenesis of FTD. However, more than half the cases of sporadic FTD show no tau deposition. We question whether altered expression is also involved in the pathogenesis of tau-negative FTD. Real-time polymerase chain reaction was used to investigate tau isoform expression in tau-negative FTD and age-matched controls. There were no differences in total tau mRNA or 4R versus 3R isoform expression. Our study suggests that perturbed tau mRNA expression is unlikely to be involved in the pathogenesis of tau-negative FTD.     

头孢克洛在健康人体内的药物动力学研究

采用ＨＰＬＣ测定健康志愿者血浆中头孢克洛的浓度。方法简便快速，结果准确。平均回收率为１０２．１８±４．０１％，日间及日内ＲＳＤ均小于６％。血药浓度－时间曲线符合一室模型。