全文获取类型
收费全文 | 7851篇 |
免费 | 354篇 |
国内免费 | 49篇 |
专业分类
耳鼻咽喉 | 49篇 |
儿科学 | 97篇 |
妇产科学 | 81篇 |
基础医学 | 1308篇 |
口腔科学 | 106篇 |
临床医学 | 501篇 |
内科学 | 2313篇 |
皮肤病学 | 31篇 |
神经病学 | 769篇 |
特种医学 | 294篇 |
外科学 | 1023篇 |
综合类 | 30篇 |
预防医学 | 164篇 |
眼科学 | 117篇 |
药学 | 542篇 |
中国医学 | 22篇 |
肿瘤学 | 807篇 |
出版年
2023年 | 35篇 |
2022年 | 80篇 |
2021年 | 170篇 |
2020年 | 78篇 |
2019年 | 110篇 |
2018年 | 148篇 |
2017年 | 101篇 |
2016年 | 147篇 |
2015年 | 168篇 |
2014年 | 212篇 |
2013年 | 294篇 |
2012年 | 415篇 |
2011年 | 494篇 |
2010年 | 283篇 |
2009年 | 244篇 |
2008年 | 460篇 |
2007年 | 491篇 |
2006年 | 490篇 |
2005年 | 432篇 |
2004年 | 438篇 |
2003年 | 481篇 |
2002年 | 508篇 |
2001年 | 149篇 |
2000年 | 127篇 |
1999年 | 187篇 |
1998年 | 138篇 |
1997年 | 115篇 |
1996年 | 104篇 |
1995年 | 72篇 |
1994年 | 106篇 |
1993年 | 72篇 |
1992年 | 98篇 |
1991年 | 84篇 |
1990年 | 70篇 |
1989年 | 99篇 |
1988年 | 63篇 |
1987年 | 61篇 |
1986年 | 57篇 |
1985年 | 54篇 |
1984年 | 56篇 |
1983年 | 34篇 |
1982年 | 19篇 |
1981年 | 24篇 |
1980年 | 28篇 |
1979年 | 22篇 |
1978年 | 23篇 |
1977年 | 15篇 |
1975年 | 12篇 |
1974年 | 11篇 |
1973年 | 10篇 |
排序方式: 共有8254条查询结果,搜索用时 0 毫秒
51.
Satoshi Fujishita Noritoshi Shibuya Norio Niikawa Shigenobu Nagataki 《Journal of human genetics》1991,36(4):317-324
Polymerase chain reaction (PCR)-based diagnosis was carried out in 62 patients (57 probands) with Duchenne or Becker muscular dystrophy (DMD or BMD) and 226 members in 57 families. The PCR studies were also performed for carrier detection in 57 mothers and 58 sisters, and prenatal diagnosis of 4 fetuses at risk of DMD. The PCR with 7 sets of primers, which amplify 7 different exon-sequences of the dystrophin gene, detected gene deletion of at least one exon in 49% of the probands. The PCR with the other 4 primer sets, which amplify 3 intragenic loci, and subsequent endonuclease digestion detected in 84% of the mothers a heterozygous pattern in at least one such locus/segment. Using the same primer sets, carrier detection was successful in 5 sisters of familial DMD cases, while recombination between the ERT87 and the 3 end intragenic loci was observed in 11% of family members studied. Prenatal diagnosis was made in all the 4 fetuses; two males were affected, one male fetus non-affected, and the remaining one female fetus a carrier. Thus, the PCR study and the primers used in the present study are useful and convincing for rapid diagnosis of DMD and/or BMD. 相似文献
52.
Han-Xiang Deng Jia-Hui Xia Mutsuo Ishikawa Norio Niikawa 《Journal of human genetics》1990,35(3):245-251
Parental origin and mechanism of formation of X chromosome structural anbormalities were studied in one each case of dup(X)(pter p11.4::p22.1qter), del(X)(qterp11:), i(X)(qtercenqter), and inv dup(X) (pterq22::q22pter) using various X-linked RFLPs as genetic markers. Segregation and densitometric analyses on polymorphic DNAs revealed that the dup(Xp) and the del(Xp) are both of paternal origin and the i(Xq) and i dic(X) are of maternal origin. The dup(Xp) had arisen by an unequal sister chromatid exchange and the del(Xp) had occurred through an intrachromosomal breakage-reunion mechanism, both in the paternal X chromosome. The i(Xq) had arisen either through centromere fission of a maternal X chromosome, followed by duplication, of its long-arm, or through a translocation between two maternal X chromosomes after meiotic crossing-over. The inv dup(X) arose through sister chromatid breakage and reunion in a maternal X chromosome. These results, together with those of previous studies, suggest that thede novo abnormalities due to events involving centromere disruption arise predominantly during oogenesis, while those due to simple breakage-reunion events occur preferentially during spermatogenesis. 相似文献
53.
K. Haukipuro N. Keränen E. Koivisto R. Lindholm R. Norio L. Punto 《Clinical genetics》1978,13(6):471-476
In a Finnish kindred consisting of 192 descendants from two marriages of a male ancestor born in 1868, the lumbar spines of 105 of the 170 living members were X-rayed. Spondylolysis was found in 22 individuals. In addition, six of them had spondylolisthesis, four had spina bifida occulta, and two had a transitional lumbar/sacral vertebra. Seven members of the kindred without spondylolysis had spina bifida occulta and 10 had transitional lumbar vertebrae.
The pedigree is consistent with autosomal dominant inheritance and incomplete (about 75 %) penetrance for spondylolysis. It raises the question of a common aetiology for several congenital disturbances in the formation of lumbar vertebrae and possibly supports the concept of a variable expressivity of a "spondylolysis gene". 相似文献
The pedigree is consistent with autosomal dominant inheritance and incomplete (about 75 %) penetrance for spondylolysis. It raises the question of a common aetiology for several congenital disturbances in the formation of lumbar vertebrae and possibly supports the concept of a variable expressivity of a "spondylolysis gene". 相似文献
54.
55.
Hoshino S Ohkoshi N Ishii A Shoji S 《Journal of muscle research and cell motility》2002,23(2):139-145
We investigated the expression of neuronal nitric oxide synthase (nNOS) and dystrophin in the regenerating skeletal muscles
of rats after cardiotoxin-induced myonecrosis by immunohistochemical studies and western blot analysis. In normal muscles,
nNOS was moderately immunostained on type 2B fibers, but was faintly immunostained on type 2A or type 1 fibers. In immunohistochemical
studies of regenerating muscles, nNOS was first observed at the sarcolemma of type 2B fibers on day 10, when the type discrimination
between types 2A and 2B was first detected by ATP reactions. Subsequently, the immunostaining of nNOS grew progressively stronger
in type 2B fibers, with faint staining in type 2A and type 1 fibers until day 28. Meanwhile, the immunostaining of dystrophin
grew stronger equally in all three fibers until day 21. In western blot analysis of regenerating muscles, nNOS regenerated
more slowly than dystrophin. The present data suggest that the expression of nNOS is related to the muscle fiber type differentiation,
and that the role of nNOS is related to the function of the type 2B fibers of the muscle.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
56.
57.
The dopamine agonist cabergoline provides neuroprotection by activation of the glutathione system and scavenging free radicals 总被引:4,自引:0,他引:4
Yoshioka M Tanaka Ki Miyazaki I Fujita N Higashi Y Asanuma M Ogawa N 《Neuroscience research》2002,43(3):259-267
Free radicals are involved in the pathogenesis and/or progression of Parkinson's disease (PD). Several ergot derivative dopamine (DA) agonists have been reported to scavenge free radicals in vitro and to show a neuroprotective effect in vivo. We investigated the in vitro free radical scavenging and antioxidant activities of cabergoline, a long-acting ergot DA agonist, as well as its ability to activate glutathione (GSH), catalase (Cat) and superoxide dismutase (SOD) activating effects and its in vivo neuroprotective properties against 6-hydroxydopamine (6-OHDA) intracerebroventricularly (i.c.v.) in mice. The striatal DA turnover induced by i.c.v. injection of 6-OHDA was completely normalized by pretreatment with cabergoline. Moreover, cabergoline scavenged free radicals in vitro and significantly reduced lipid peroxidation in vitro and in vivo. Furthermore, daily administration of cabergoline to mice significantly increased striatal GSH levels by activation of RNA expressions of GSH-related enzymes, although striatal Cat and SOD activities did not change. In addition, our present results suggest that repeated administration of cabergoline attenuates both 6-OHDA-induced nigrostriatal DAergic dysfunction and DA neuronal cell death, since cabergoline also had a neuroprotective effect in the immunohistochemical experiment. In conclusion, our findings indicate that the multiple antioxidant mechanisms of cabergoline, such as activation of the GSH system and the direct free radical scavenging activity, may explain the neuroprotective effect of this ergot DA agonist. 相似文献
58.
Kiyoshi Imaizumi Junko Kimura Mari Matsuo Kenji Kurosawa Mitsuo Masuno Norio Niikawa Yoshikazu Kuroki 《American journal of medical genetics》2002,107(1):58-60
We describe a de novo balanced reciprocal translocation between the long arms of chromosomes 5 and 8 [46,XX,t(5;8)(q35;q24.1)] in a 15-month-old girl with a typical Sotos syndrome phenotype. Involvement of the 5q35 region was previously reported (Maroun et al. [1994: Am J Med Genet 50:291-293]) as one of translocation breakpoints in the present patient. We suggest that the gene responsible for Sotos syndrome is located to a distal long-arm region of chromosome 5. 相似文献
59.
Involvement of Rho-kinase in inflammatory and neuropathic pain through phosphorylation of myristoylated alanine-rich C-kinase substrate (MARCKS) 总被引:2,自引:0,他引:2
Tatsumi S Mabuchi T Katano T Matsumura S Abe T Hidaka H Suzuki M Sasaki Y Minami T Ito S 《Neuroscience》2005,131(2):491-498
Myristoylated alanine-rich C-kinase substrate (MARCKS) is a major in vivo substrate for protein kinase C in the brain and has been implicated in cellular processes associated with cytoskeletal restructuring such as synaptic trafficking and neurotransmitter release. A phosphorylation-site specific antibody against Ser159-phospho-MARCKS (pS159-Mar-Ab) revealed that MARCKS is phosphorylated at Ser159 by Rho-kinase and that its phosphorylation is inhibited by the Rho-kinase specific inhibitor H-1152. Since the function of MARCKS is regulated by phosphorylation at multiple sites, here we examined the involvement of Rho-kinase in relation to phosphorylation of MARCKS at Ser159 in inflammatory and neuropathic pain by H-1152. When intrathecally administered 10 min before s.c. injection of formalin, H-1152 at 10 and 100 ng attenuated the second-phase, but not the first-phase, pain-like behaviors in the formalin test. Neuropathic pain induced by selective L5 spinal nerve transection was also relieved by intrathecal injection of H-1152. Nitric oxide synthase activity visualized by NADPH diaphorase histochemistry increased in the superficial layer of the spinal cord 30 min after formalin injection and 7 days after nerve transection, which were blocked by H-1152. Phosphorylation of MARCKS at Ser159 was detected in the spinal cord by pS159-Mar-Ab and the level of phosphorylation increased in the superficial layer after nerve transection. In contrast, immunoreactivities of neuronal nitric oxide synthase and MARCKS did not change significantly in the spinal cord before and after nerve transection. Taken together, the present study demonstrates that Rho-kinase is involved in inflammatory pain and the maintenance of neuropathic pain through phosphorylation of MARCKS at Ser159. 相似文献
60.
Roger Sciammas Y. Tatsumi A. I. Sperling K. Arunan Jeffrey A. Bluestone PhD 《Immunologic research》1994,13(4):268-279
γδ cells participate in pathogenic infections and autoimmune conditions, yet, almost a decade after their discovery, little
is known regarding their TCR repertoire or effector functions. Unlike MHC-restricted antigen recognition employed by TCRαβ
cells, TCRγδ cells can recognize whole unprocessed antigens in an MHC-independent manner. The nature of positive and negative
selection used to shape the repertoire of TCRγδ cells is unclear, especially in the nonlymphoid tissues where these cells
predominate. While TCRγδ cells express an activated phenotype and are present in pathological conditions, their roles in immunological
protection is unknown. This review will focus on our efforts to study these issues of TCRγδ biology. 相似文献