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11.
12.
Rapid chemical kinetic techniques for investigations of
neurotransmitter receptors expressed
in Xenopus oocytes 下载免费PDF全文
Li Niu RaymondW. Vazquez Georg Nagel Thomas Friedrich Ernst Bamberg RobertE. Oswald GeorgeP. Hess 《Proceedings of the National Academy of Sciences of the United States of America》1996,93(23):12964-12968
Xenopus laevis oocytes have been used extensively during the past decade to express and study neurotransmitter receptors of various origins and subunit composition and also to express and study receptors altered by site-specific mutations. Interpretations of the effects of structural differences on receptor mechanisms were, however, hampered by a lack of rapid chemical reaction techniques suitable for use with oocytes. Here we describe flow and photolysis techniques, with 2-ms and 100-μs time resolution, respectively, for studying neurotransmitter receptors in giant (≈20-μm diameter) patches of oocyte membranes, using muscle and neuronal acetylcholine receptors as examples. With these techniques, we find that the muscle receptor in BC3H1 cells and the same receptor expressed in oocytes have comparable kinetic properties. This finding is in contrast to previous studies and raises questions regarding the interpretations of the many studies of receptors expressed in oocytes in which an insufficient time resolution was available. The results obtained indicate that the rapid reaction techniques described here, in conjunction with the oocyte expression system, will be useful in answering many outstanding questions regarding the structure and function of diverse neurotransmitter receptors. 相似文献
13.
Action of the drug Herba Epimedii on testosterone of the mouse plasma and its accessory sexual organ before and after processing 总被引:1,自引:0,他引:1
In this report, the traditional idea that sheep fat processed Herba Epimedii warms the kidney and thus invigorate "Yang" has been tested and verified by modern pharmacological methods. Experimental findings indicate that raw Herba Epimedii does not help improve the sexual function, while well processed Herba Epimedii produces apparent effects on the improvement of sexuality. 相似文献
14.
甲襞、球结膜微循环的应急观测方法是在甲襞、球结膜微循环加权积分综合定量评价方法的基础上,保留权值为4、3的指标,同时观察血管清晰度和红细胞聚集二项指标而形成。同时提供其应急观测积分表、异常分度积分值、诊断标准,并与常规方法进行比较,证明其可以反映绝大部分流态类指标和大部分形态类指标及重要的袢周改变,没有丢失微循环重度异常的信息,保证了重度异常诊断的正确。但在大致正常、轻度异常和中度异常的诊断中应急方法部有判重的倾向,说明应急方法漏掉了一些信息,不能全面地了解微循环的改变,造成了判重的倾向。因此应急方法不能代替常规方法,只能在特定条件下应用。 相似文献
15.
Helen Pineo Camilla Audia Daniel Black Matthew French Emily Gemmell Gina S. Lovasi James Milner Felipe Montes Yanlin Niu Carolina Prez-Ferrer Jos Siri Ruzka R. Taruc 《Journal of urban health》2021,98(3):442
Anthropogenic environmental change will heavily impact cities, yet associated health risks will depend significantly on decisions made by urban leaders across a wide range of non-health sectors, including transport, energy, housing, basic urban services, and others. A subset of planetary health researchers focus on understanding the urban health impacts of global environmental change, and how these vary globally and within cities. Such researchers increasingly adopt collaborative transdisciplinary approaches to engage policy-makers, private citizens, and other actors in identifying and evaluating potential policy solutions that will reduce environmental impacts in ways that simultaneously promote health, equity, and/or local economies—in other words, maximising ‘co-benefits’. This report presents observations from a participatory workshop focused on challenges and opportunities for urban planetary health research. The workshop, held at the 16th International Conference on Urban Health (ICUH) in Xiamen, China, in November 2019, brought together 49 participants and covered topics related to collaboration, data, and research impact. It featured research projects funded by the Wellcome Trust’s Our Planet Our Health (OPOH) programme. This report aims to concisely summarise and disseminate participants’ collective contributions to current methodological practice in urban planetary health research. 相似文献
16.
目的 了解郑州市新型冠状病毒肺炎患者定点收治医院护理人员的心理状况,从而为应对突发公共卫生事件医务人员的心理卫生、心理干预咨询提供参考的理论依据及指导.方法 选择本市新型冠状病毒肺炎疑似及确诊患者定点救治医院护理人员,共收集到462名护理人员信息,采用填写网络问卷SCL-90量表的方法进行调查,并与常模数据做对比,用SPSS19.0统计软件进行统计分析.结果 最终完成436名护理人员调查统计与分析,SCL-90量表总得分为(144.51±36.20),高于1986年全国常模(129.96±38.26)分以及2006年全国常模(130.021±33.626)分.其中,躯体化、强迫、抑郁、焦虑、恐怖因子分高于护士常模(P<0.05)及全国常模(P<0.05).结论 新型冠状病毒肺炎疫情期间,本市护理人员存在一定的心理压力.我们应注意到护理人员的心理压力以及引起压力的主要危险因素,并采取积极有效的方式应对,采取针对性措施进行疏导,减轻护理人员的心理压力,保证护理人员工作有效顺利进行. 相似文献
17.
18.
目的:研究低功率氦氖激光对小鼠小脑信息传递物质的影响。方法:采用雄性BALB/C小鼠,随机分为对照组,辐射后即刻组、1h组、2h组,注入氮氖合酶(NOS)抑制剂的辐射后即刻组、副射后1h组,用波长632.8nm、功率密度5mW/cm^2连续氦氖激光每天局部辐射1次,每次1h,连续5d。对照辐射功率密度为0。结果:辐射组分别于辐射后即刻、1h小脑组织中一氧化氮(NO)代谢产物亚硝酸根(NO2^-)及环磷酸鸟苷(cGMP)含量均升高(P<0.01),辐射2h二者均降至正常,此增高效应可同时被NOS抑制剂阻断。结论:低功率氦氖激光可使小鼠小脑NO代谢产物NO2^-及cGMP含量短暂性升高,在氦氖激光辐射产生的生物学效应中涉及NO/cGMP信息传递机制。 相似文献
19.
Acute leukemia is a clonal malignancy ofhematopoietic tissue characterized by proliferation ofabnormalblastcellsin the bone marrow and impairedproduction of normal blood cells.Magnetic resonanceimaging( MRI) isuseful in characterizing normal andabnormal bone marrow ( BM) because of its ability todistinguish fatfrom denser,more cellulartissuessuchas leukemia.MRIalso presentsa more global view ofthe BM than biopsy materials and,therefore,mayprovide a better understanding of diffuse hematopoi-… 相似文献
20.
尿液结合性胆红素的纯化 总被引:2,自引:1,他引:1
OBJECTIVES: To develop a simple, practical method with fine yield and purity for separating conjugated bilirubin(Bc) from a sustainable and facile material in order that it could serve as a calibrator for total and direct bilirubin determination. METHODS: Isolating Bc from urine of patients with obstructive jaundice by CHCl3: CH3CH2OH(1:1, V/V) followed by CNBr activated Sepharose 4B-HAS affinity chromatography, we analyzed the products by the use of Doumas J-G diazo method and HPLC, respectively. RESULTS: Determined by J-G diazo method, the crude product extracted by CHCl3: CH3CH2OH(1:1, V/V) yields 90.2 +/- 5.4%(n = 3) of urine Bc, the ratio of Bc to total dry weight being 67.9 +/- 6.4%(n = 3). The final product purified by affinity chromatography with the ratio of separation of 1.5, 1.737 and analyzed by HPLC has the yield of 46.9% and purity of 94.7%(BDG of 76.2%, BMG of 18.5%). CONCLUSION: The above data demonstrate that urine conjugated bilirubin can be successfully purified by CHCl3: CH3CH2OH extraction followed by Sephrose-HSA affinity chromatography with sufficient purity and output. 相似文献